• The Korean Journal of Physiology and Pharmacology
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• v.27 no.5
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• pp.457-470
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• 2023

The aim of this study was to investigate the role of kinesin superfamily member 15 (KIF15) in nasopharyngeal carcinogenesis (NPC) and explore its underlying mechanisms. We employed various assays, including the CCK-8 assay, flow cytometry, the Transwell and scratch assay, Western blotting, and nude mice transplantation tumor, to investigate the impact of KIF15 on NPC. Our findings demonstrate that KIF15 plays a critical role in the proliferation, apoptosis, migration, and invasion of NPC cells. Furthermore, we discovered that silencing KIF15 inhibits cell proliferation, migration, and invasion while promoting apoptosis, and that KIF15's effect on NPC cell growth is mediated through the PI3K/AKT and P53 signaling pathways. Additionally, we showed that KIF15 promotes nasopharyngeal cancer cell growth in vivo. Our study sheds light on the significance of KIF15 in NPC by revealing that KIF15 knockdown inhibits NPC cell growth through the regulation of AKT-related signaling pathways. These findings suggest that KIF15 represents a promising therapeutic target for the prevention and treatment of NPC.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2022.05a
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• pp.141-143
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• 2022

This paper presented a new technology to identify traffic violations of motorbike riders by detecting the traffic signal using You Only Look Once (YOLO) object detection. The hardware module that is mounted on the front of the motorbike consists of Raspberry Pi with a camera to run the YOLO object detection, a GPS module to acquire the motorcycle's coordinate, and a LoRa communication module to send the data to a cloud DB. The main goal of the software is to determine whether a motorbike has violated a traffic signal. This paper proposes a function to recognize the red traffic signal colour with its movement inside the camera angle and determine that the traffic signal violation happens if the traffic signal is moving to the right direction (the rider turns left) or moving to the top direction (the riders goes straight). Furthermore, if a motorbike rider is violated the signal, the rider's personal information (name, mobile phone number, etc), the snapshot of the violation situation, rider's location, and date/time will be sent to a cloud DB. The violation information will be delivered to the driver's smartphone as a push notification and the local police station to be used for issuing violation tickets, which is expected to prevent motorbike riders from violating traffic signals.

• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.452-462
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• 2021

Pretilachlor (PRE) is a common acetanilide herbicide used worldwide. However, its effects on aquatic organisms, particularly marine photosynthetic life, are not sufficiently known. Herein, we evaluated the toxic effects of PRE by physiological and molecular parameters in the photosynthetic dinoflagellate Prorocentrum minimum. The cell density, pigment content, and photosynthetic parameters (F_v/F_m and PI_ABS) were considerably decreased with increased PRE exposure time and doses. In addition, photosynthesis-related genes, PmpsbA, PmpsaA, and PmatpB, were significantly upregulated when exposed to 1.0 mg L^-1 of PRE for 24 h (p<0.001). In 72 h treatment, the relative gene expression was significantly increased (0.1 and 0.5 mg L^-1; p<0.01). In contrast, PmrbcL was decreased or little changed compared to the controls. Reactive oxygen species (ROS) increased after 24 h exposure (p<0.001). However, the transcriptional fold-changes in glutathione S-transferase (GST) were significantly increased (0.5 and 1.0 mg L^-1; p<0.001) at 72 h. These findings suggested that the PmGST might be involved in PRE detoxification in P. minimum. In addition, PRE may affect the photosystem function in phytoplankton similar to other acetanilides, causing severe damage or cell death.

• Korean Journal of Animal Reproduction
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• v.23 no.2
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• pp.133-139
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• 1999

This study was carried out to investigate in vitro/in vivo development of vitrified-thawed immature mouse oocytes. Immature mouse oocytes were vitrified with EFS40 (40% ethylene glycol, 18% ficoll and 0.5 M sucrose). Thawed oocytes were matured for 16 hr in vitro. Matured oocytes with the first polar body were fertilized with the concentration of 1~2$\times$10$^{6}$ $m\ell$ of epididymal sperm. After fertilization, cleavage ($\geq$ 2-cell) and in vitro/in vivo development rates were examined. $\pi$ Ie results were summarized as follows: in vitro maturation rate of immature mouse oocytes in vitrified-thawed group was similar to that in exposed group (67.5%) and control (66.3%), but cleavage rate of vitrified-thawed oocytes (64.9 %) and blastocyst formation rate (59.0%) were significantly different compared to those of exposed group (83.7 and 74.7%) and control (90.7 and 83.7%) (p＜0.05). However, when the blastocysts derived from immature mouse oocytes vitrified-thawed were transferred to pseudopregnant mouse, total implantation (31.3%) was slightly lower than that in control (40.8%), but live fetus formation rate (66.7%)was slightly higher than that in control (58.1%), there was not significantly different. Therefore, when the blastocyts produced in vitro were transferred into recipients, although the development in vitro of oocytes vitrified-thawed was decreased, live fetus formation rate was similar to that of control group. The present results indicate that immature mouse oocytes can be frozen successfully by vitrification with EFS40.

• The Journal of Korean Medicine
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• v.44 no.2
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• pp.106-118
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• 2023

Objectives: Network pharmacology is a method of constructing and analyzing a drug-compound-target network to predict potential efficacy and mechanisms related to drug targets. In that large-scale analysis can be performed in a short time, it is considered a suitable tool to explore the function and role of herbal medicine. Thus, we investigated the potential functions and pathways of Chongmyunggongjin-dan (CMGJD) on Alzheimer's disease (AD) via network pharmacology analysis. Methods: Using public databases and PubChem database, compounds of CMGJD and their target genes were collected. The putative target genes of CMGJD and known target genes of AD were compared and found the correlation. Then, the network was constructed using Cytoscape 3.9.1. and functional enrichment analysis was conducted based on the Gene Ontology (GO) Biological process and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathways to predict the mechanisms. Results: The result showed that total 104 compounds and 1157 related genes were gathered from CMGJD. The network consisted of 1157nodes and 10034 edges. 859 genes were interacted with AD gene set, suggesting that the effects of CMGJD are closely related to AD. Target genes of CMGJD are considerably associated with various pathways including 'Positive regulation of chemokine production', 'Cellular response to toxic substance', 'Arachidonic acid metabolic process', 'PI3K-Akt signaling pathway', 'Metabolic pathways', 'IL-17 signaling pathway' and 'Neuroactive ligand-receptor interaction'. Conclusion: Through a network pharmacological method, CMGJD was predicted to have high relevance with AD by regulating inflammation. This study could be used as a basis for effects of CMGJD on AD.

• Journal of Digital Convergence
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• v.20 no.3
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• pp.333-341
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• 2022

Despite rapidly increasing demand for fishing, there is a lack of studies and information related to fishing, and there is a limit to obtaining the data on the global distribution of fish resources. Since the existing method of investigating fish resource distribution is designed to collect the fish resource information by visiting the investigation area using a throwing net, it is almost impossible to collect nation-wide data, such as streams, rivers, and seas. In addition, the existing method of measuring the length of fish used a tape measure, but in this study, a FishingTAG's smart measure was developed. When recording a picture using a FishingTAG's smart measure, the length of the fish and the environmental data when the fish was caught are automatically collected, and there is no need to carry a tape measure, so the user's convenience can be increased. With the development of a global fishing application using a FishingTAG's smart measure, first, it is possible to collect fish resource samples in a wide area around the world continuously on a real time basis. Second, it is possible to reduce the enormous cost for collecting fish resource data and to monitor the distribution and expansion of the alien fish species disturbing the ecosystem. Third, by visualizing global fish resource information through the Google Maps, users can obtain the information on fish resources according to their location. Since it provides the fish resource data collected on a real time basis, it is expected to of great help to various studies and the establishment of policies.

• Communications of Mathematical Education
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• v.36 no.1
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• pp.171-199
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• 2022

In this study, we review contents to supplement the descriptions of the history of mathematics in the 2015 mathematics textbooks and teacher guides for the elementary school level and offer our opinion on them. For this purpose, we conducted a literature review on 24 types of 2015 mathematics textbooks and teacher guides for the elementary school level. The results of this study are as follows: A total of 10 topics were found whose contents were supplemented with descriptions. They were the "Arithmetic of the Ancient Egyptians," the "A'h-mosè Papyrus in Mathematics Textbooks of the Ancient Egyptians," "The Old Akkadian Square Band in Mesopotamia," "The Relationship of the Old Babylonians in Mesopotamia with the Angle," "The Pi of the Ancient Egyptians and the Old Babylonians," "The Square Roots 2 of the Ancient Egyptians and the Old Babylonians," "The Relationship of the Islamites with the Decimal Fraction," "Two Arguments for the Roots of the Golden Ratio," "The Relationship of Archimedes with the Exhaustion Method," and "The Design of Flats." Then, their specific supplements were suggested. It is expected that this will overcome the perspective of the history of the Axial Age and acknowledge and accept the perspective evidencing the transfer of mathematical culture from Ancient Egypt and Old Babylonia to Ancient Greece and Hellenism, and then through Central Asia to Europe.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.43-43
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• 2021

Sesan강과 Srepok강은 베트남, 캄보디아, 라오스가 공유하는 3S강 유역 (Sesan강, Srepok강, Sekong강)의 일부로 국제 공유하천으로 관리되고 있다. 3S강 유역은 Mekong강의 중요한 지류이며 Mekong강 유역의 상당 부분을 구성한다(Mekong강 유역 면적의 10%, 연간 총 유출량의 20%). 베트남에 속해 있는 Sesan강 유역면적은 11,255km², Srepok강 유역면적은 18,162km²이다. Sesan강과 Srepok강의 상류는 베트남 중부 고원의 긴 산맥에 위치하고 있으며, 하류는 캄보디아에 위치해 있어 상·하류간 긴밀한 협력이 필요하다. Sesan강과 Srepok강 유역은 기후변화에 따른 홍수, 가뭄, 수력발전소 건설로 인한 유출량 변동에 따른 상·하류 분쟁, 사면침식 및 퇴적 등 많은 문제와 도전에 직면할 것으로 예측되고 있다. 본 연구에서는 World Bank의 "Viet Nam Mekong Integrated Water Resources Management (M-IWRM) Project의 일환으로 베트남 정부 차원에서 처음으로 구축한 수자원관리 의사결정지원시스템인 "DSS-2S"를 활용하여, Sesan-Srepok강 유역의 수자원 계획을 수립하였다. DSS-2S는 MIKE Hydro Basin을 기반으로 SWAT모델 등과 연계 하여 구축되었다. DSS-2S는 2S 유역의 모든 주요 하천과 지류를 반영하였으며. 여기에는 17개의 수력발전 댐과 주요 지류에서 용량이 3백만 m³ 이상인 기타 저수지가 포함되었다. 이 보다 작은 용량의 저수지는 대표적인 저수지로 그룹화 되어 반영되었다. 기후변화 및 사회-경제적 발전계획 등을 반영하여, 2030년과 2050년을 목표연도로 생활, 공업, 농업, 관광, 유지용수 등 용수 수요를 추정하였다. 50% 및 85% 빈도의 공급 가능성을 고려하여 물 배분은 물 수요를 충족하고 지하수 개발 최소화를 기준으로 고려되었다. 분석 결과에 의하면 2S강 유역의 총 수자원은 32.2억 m³으로 그중 지표수자원은 29.2억 m³, 안정적으로 이용 가능한 지하수자원은 2.97억 m³으로 분석 되었으며, 지표수와 지하수 연계를 고려하면 전체 2S 강 유역에 물 부족하지는 않으나, 개별 공급 지점을 고려할 때 4월과 5월에 일부 지역에서 물 부족이 나타날 것으로 예측 된다. 장래 물 부족 해결을 위한 대안들을 제시하였으며, 본 성과는 베트남 중앙 정부의 장기수자원 종합계획 수립의 기본 자료로 활용 될 예정이다.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.453-453
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• 2021

도시지역 비점오염원관리, 물순환 회복, 침투 및 증발산량 증가, 열섬현상 저감을 위한 주요한 방안으로 저영향개발(low impact development, LID)과 그린인프라 기법의 적용되고 있다. LID 시설은 소규모 분산형 시설로써 넓은 지역에 많고 다양한 시설들이 적용되어 시설의 개수가 많으며, 수질 및 토양 내 기성제품에 대한 센서들의 가격은 고가로 형성되어 있어 기기의 경제성 및 유지관리 등 적용하는데 제한적이다. 따라서 과거 모니터링 자료를 기반으로 오염물질들과의 상관성 분석을 통하여 계측이 어려운 항목들을 계측가능한 항목들로부터 예측 가능하며, 선정된 항목들에 대한 비용효율적인 센서를 개발하여 실시간 LID 모니터링이 가능한 비용효율적 모니터링을 개발하였다. 공주대학교 천안캠퍼스의 LID 시설들은 2013년에 조성되어 현재까지 시설이 운영되고 있으며, 5년이상의 과거 강우시 모니터링 자료들을 이용하여 오염물질 상관성 분석을 수행가능 하기에 대상지로 선정하였다. 오염물질 상관성 분석은 2013년부터 2017년도에 침투도랑에서 수행된 강우시 모니터링 자료를 활용하여 각 오염물질들의 상관성을 분석을 수행하였다. 침투도랑 내 유입되는 평균 유입수는 TSS 286.1±318.3 mg/L, BOD 22.6±39.5 mg/L, TN 8.96±5.85 mg/L, TP 1.01±1.11 mg/L로 나타났다. 겨울철에 비해 여름철에서의 오염물질의 유입농도가 높은 것으로 분석되었다. 이는 여름철 고온건조로 인한 노면 내 차량의 주행으로 인한 중금속, 폐타이어 등과 장마철 강우 시 유출된 토사로 인하여 유입수의 농도가 높은 것으로 분석되었다. 오염물질 부하량은 TSS와 COD 0.66으로 유의성이 높은 것으로 나왔으며, COD와 TSS, TP, TN 등 유의성이 높은 것으로 분석되었다. Arduino와 Raspberry PI를 활용하여 저비용 센서와 LTE 모뎀통신과 데이터 베이스 연결하여 개발된 프로그램을 통해서 무선으로 LID 시설에 대한 모니터링을 침투화분2와 식생체류지에 조성하였다. 전력공급이 어려운 식생체류지의 경우 태양열(Solar system) 시스템과 보조 전력 배터리를 조성하여 장마철이나 장기적인 악천후로 인한 전력을 생산하지 못할 경우 보조전력배터리에서 전력을 제공하여 지속적인 모니터링이 이루어지도록 설계하였다. 토양함수량, 토양온도와 Conductivity 등 3종류의 센서를 적용하였으며, 프로그램은 현재 2단계를 통한 2차수정을 통하여 프로그램을 구축하였다. 오차, 오작동, 계측값에 대한 검·보정 작업이 필요하다. 또한 대기자료의 구축을 통해 보다 토양과 LID 시설에 대한 영향분석이 필요한 것으로 사료된다.

• Korean Journal of Heritage: History & Science
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• v.56 no.1
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• pp.118-130
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• 2023

Decay or large cavities inside trees are the main causes of trees overturning and broken branches, and structurally weakened trees are more vulnerable to strong winds and heavy snowfall. Recently, as strong winds and typhoons increase due to climate change, the damage to human life and property due to trees overturning continues to increase, and cultural assets are in a similar situation. In particular, old big trees are structurally vulnerable to external shocks such as strong winds and heavy snowfall. This study was aimed at providing a scientific basis for preventive protection measures by conducting a structural stability diagnosis of seven retusa fringe trees designated as natural monuments. For the structural stability diagnosis, tree risk assessment and internal tree defect measurements were performed. As a result of the tree risk assessment, the Retusa Fringe Trees in Sinjeon-ri, Yangsan and Gwangyangeupsu had the highest risk of broken branches due to weak branch attachment strength. As a result of the diagnosis of internal defects of cross sections of measured trees, there were suspected cavities or severe decay in all except two trees of the population of Retusa Fringe Trees in Pyeongji-ri. Natural disasters due to climate change are increasing, and the scale is getting larger, so it is very important to preemptively manage large old trees through scientific structural safety diagnosis to manage trees that are vulnerable to environmental changes.