• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.12
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• pp.426-433
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• 2020

This study aimed to compare the pretreatment methods of phytoplankton for type approval of the Ballast Water Treatment System (BWMS). The International Maritime Organization (IMO) and the United States Maritime Police (USCG) use two different test methods for this purpose. To compare the two methods, a test for concentration and non-concentration was performed with cultured and natural phytoplankton, and samples from the land-based BWMS test. The extent of damages caused by the process of concentration varied between cultured and natural species, indicating differences depending on the physiological and morphological characteristics of the species. In the land-based test, in the control water with a high biological population, the number of non-concentrated samples was about twice as high as that of the concentrated samples. There was no distinct difference between the two methods in the treated water with a low biological population. Thus, although there is a difference between concentration and non-concentration for phytoplankton sampling, the concentration method can be applied as a method of evaluating BWMS performance. However, a method for evaluating whether live species in treated water may be lost or damaged during the concentration process of sampling should be developed and validated.

• Journal of Life Science
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• v.12 no.3
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• pp.250-255
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• 2002

Fluorescent species-specific DNA probe (AT1) of toxic dinoflagellate Arexandrium tamarense was tested on several other species, on comparison of binding activity at different preservatives for fixation of the cells, at different culture age and estimation of cell density by light microscope or epifluorescent microscope using whole cell hybridization. Th AT1 probe specifically bound to Alexandrium tamarense, whereas it did not bind to other phytoplankton, in particular Alexandrium catenella, morphologically similar to Alexandrium tamarense, could not react to AT1 probe. When cells were fixed with all three preservatives, labeling cells of Alexandrium tamarense emitted strong fluorescent signal intensity. In addition, regardless culture days, binding activity with AT1 probe was strong. The tell densities estimated by epifluorescent microscope were than those estimated by light microscope. The enumeration and identifying of Arexandriurn tamarense using DNA probe method will be contributed to a new biotoxin monitoring and prediction system in field.