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http://dx.doi.org/10.5352/JLS.2002.12.3.250

Application of Species-specific DNA Probe to Field Samples of Alexandrium tamarense (Lebour) Balech  

Cho, Eun-Seob (Harmful Algal Research Division, National Fisheries Research and Development Institute)
Kim, Gi-Young (Department of Microbiology, Pusan National University)
Park, Hyung-Sik (Department of Microbiology, Pusan National University)
Kim, Hak-Gyoon (Harmful Algal Research Division, National Fisheries Research and Development Institute)
Moon, Sung-Ki (Department of Biology, College of Science, Kyungsung University)
Lee, Jae-Dong (Department of Microbiology, Pusan National University)
Publication Information
Journal of Life Science / v.12, no.3, 2002 , pp. 250-255 More about this Journal
Abstract
Fluorescent species-specific DNA probe (AT1) of toxic dinoflagellate Arexandrium tamarense was tested on several other species, on comparison of binding activity at different preservatives for fixation of the cells, at different culture age and estimation of cell density by light microscope or epifluorescent microscope using whole cell hybridization. Th AT1 probe specifically bound to Alexandrium tamarense, whereas it did not bind to other phytoplankton, in particular Alexandrium catenella, morphologically similar to Alexandrium tamarense, could not react to AT1 probe. When cells were fixed with all three preservatives, labeling cells of Alexandrium tamarense emitted strong fluorescent signal intensity. In addition, regardless culture days, binding activity with AT1 probe was strong. The tell densities estimated by epifluorescent microscope were than those estimated by light microscope. The enumeration and identifying of Arexandriurn tamarense using DNA probe method will be contributed to a new biotoxin monitoring and prediction system in field.
Keywords
DNA probe; Alexandrium tamarense; identification; PSP; toxication;
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