• BMB Reports
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• 제40권2호
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• pp.135-141
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• 2007

Conjugation of the methyl group at the fifth carbon of cytosines within the palindromic dinucleotide 5'-CpG-3' sequence (DNA methylation) is the best studied epigenetic mechanism, which acts together with other epigenetic entities: histone modification, chromatin remodeling and microRNAs to shape the chromatin structure of DNA according to its functional state. The cancer genome is frequently characterized by hypermethylation of specific genes concurrently with an overall decrease in the level of 5-methyl cytosine, the pathological implication of which to the cancerous state has been well established. While the latest genome-wide technologies have been applied to classify and interpret the epigenetic layer of gene regulation in the physiological and disease states, the epigenetic testing has also been seriously explored in clinical practice for early detection, refining tumor staging and predicting disease recurrence. This critique reviews the latest research findings on the use of DNA methylation in cancer diagnosis, prognosis and staging/classification.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1992년도 추계학술대회
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• pp.51-54
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• 1992

In this paper, we quantitatively evaluated the anxiety data from humans in an anxiety state. It has been reported that the electric signals of human can be correlated with their emotional state. We chose heart rate, respiration rate, temperature and skin conductance as the anxiety parameters. For experiment protocol, the subject was given exercising load to induce the anxiety state and the exercise was done using the FITRON Cycle Ergometer. We divided the data into three stages: rest period, exercise period, and recovery period. During evaluation, We counted the heart rate, slope of heart rate, temperature change, and the skin conductance.

• 사상체질의학회지
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• 제8권2호
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• pp.11-39
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• 1996

The purpose of this investigation is to seek the concepts of the Nature and the Emotion in the Sasang Constitutional Medicine. The results summerized as following. 1. When feelings like joy, anger, sorrow and happiness are kept in heart this state is the Nature. When they are expressed this state is the Emotion. In this point Confucianism and the Sasang Constitutional Medicine are same each other. 2. The Nature and Emotion of Confucianism are the psychic concepts. But the Nature and Emotion of the Sasang Constitutional Medicine are the psychosomatic concepts. 3. QI(氣) of joy, anger, sorrow and happiness, each of them share with qi(氣) of the Nature and Emotion. Qi(氣) of the Nature and Emotion directly influence the formation of Sasang Constitution, physiological and pathological state. As mentioned above, though the Nature and Emotion of the Sasang Constitutional Medicine come from Confucianism they are used the more opened conception.

• 한국자기공명학회논문지
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• 제8권1호
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• pp.55-61
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• 2004

Biosupramolecular systems such as biological membranes usually fluid under physiological conditions$^1$. Therefore, solid-state NMR has been used to investigate biosupramolecular systems. But solid-state NMR spectra contain a large number of overlapping resonances and are rather difficult to analyze. These problem has to be overcome by selective isotope labeling. We constructed a deuterium NMR probe for AM400 NMR spectrometer, which is mainly used for liquid samples. To overcome the fluidity problem, a saddle type coil was designed. The efficiency was systematically investigated for two kinds of coil geometry, solenoid and saddle types. Our results suggest that solenoids are superior to saddle type coils in the sensitivity. However, the letter fits better to fluid samples such as biosupramolecular systems.

• 한국지능시스템학회논문지
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• 제23권3호
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• pp.262-267
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• 2013

The recognition of human emotional state is one of the most important components for efficient human-human and human- computer interaction. In this paper, four emotions such as fear, disgust, joy, and neutral was a main problem of classifying emotion recognition and an approach of visual-stimuli for eliciting emotion based on physiological signals of skin conductance (SC), skin temperature (SKT), and blood volume pulse (BVP) was used to design the experiment. In order to reach the goal of solving this problem, half-against-half (HAH) multi-class support vector machine (SVM) with Gaussian radial basis function (RBF) kernel was proposed showing the effective techniques to improve the accuracy rate of emotion classification. The experimental results proved that the proposed was an efficient method for solving the emotion recognition problems with the accuracy rate of 90% of neutral, 86.67% of joy, 85% of disgust, and 80% of fear.

• 한국감성과학회:학술대회논문집
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• 한국감성과학회 2001년도 춘계학술대회 논문집
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• pp.225-230
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• 2001

Intensity of background noise is a factor significantly affecting both subjective evaluation of experienced stress level and associated electroencephalographic (EEG) responses during mental load in noisy environments. In the study on 27 subjects we analyzed the influence of the background white noise (WN) intensity on psychophysiological responses during a word recognition test. Electrocortical activity were recorded during baseline resting state and 40 s long performance on 3 similar Korean word recognition tests with different intensities of background WN (55, 70 and 85 dB).. An important finding in terms of physiological reactivity was similarity of all physiological response profiles between 55 and 70dB WN, i.e., none of physiological variables differentiated the two conditions, while 85dB WN resulted in a significantly different profile of reactions (higher fast beta power in EEG spectra). This condition was characterized by highest subjective rating of experienced stress, had more fast beta activity and had tendency of right hemisphere dominance, emphasizing the role of brain lateralization in negative affect control.

• 한국정밀공학회지
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• 제31권5호
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• pp.459-466
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• 2014

An index was developed that estimates cardiovascular health degree with easily available physiological information such as survey and noninvasive measurement. The survey score was calculated by utilizing questions related to personal disease history, self-feeling, and management state. The measurement score was calculated using physiological parameters such as blood pressure, accelerated plethysmograph(APG), and heart rate variability(HRV), and augmentation index(AI). In order to evaluate effectiveness of the cardiovascular index and modify weighting factors used in each item, a clinical trial was done in a general hospital. The cardiovascular index showed a clear correlation of 0.685 with the doctor's score on the cardiovascular health degree. The correlation between the self-estimated score and doctor's score was as low as 0.217. The large gap between these two scores demonstrated necessity of more objective tools like the cardiovascular index. The cardiovascular score showed a significant difference between normal persons and patients suffering hypertension or diabetes. (p=0.000).

• Journal of Microbiology and Biotechnology
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• 제25권2호
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• pp.152-161
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• 2015

CodY is a highly conserved protein in low G+C gram-positive bacteria that regulates genes involved in sporulation and stationary-phase adaptation. Bacillus thuringiensis is a grampositive bacterium that forms spores and parasporal crystals during the stationary phase. To our knowledge, the regulatory mechanism of CodY in B. thuringiensis is unknown. To study the function of CodY protein in B. thuringiensis, BMB171codY^- was constructed in a BMB171 strain. A shuttle vector containing the ORF of cry1Ac10 was transformed into BMB171 and BMB171codY^-, named BMB171cry1Ac and BMB171codY^-cry1Ac, respectively. Some morphological and physiological changes of codY mutant BMB171codY^-cry1Ac were observed. A comparative proteomic analysis was conducted for both BMB171codY^-cry1Ac and BMB171cry1Ac through two-dimensional gel electrophoresis and MALDI-TOF-MS/MS analysis. The results showed that the proteins regulated by CodY are involved in microbial metabolism, including branched-chain amino acid metabolism, carbohydrate metabolism, fatty acid metabolism, and energy metabolism. Furthermore, we found CodY to be involved in sporulation, biosynthesis of poly-β-hydroxybutyrate, growth, genetic competence, and translation. According to the analysis of differentially expressed proteins, and physiological characterization of the codY mutant, we performed bacterial one-hybrid and electrophoretic mobility shift assay experiments and confirmed the direct regulation of genes by CodY, specifically those involved in metabolism of branched-chain amino acids, ribosomal recycling factor FRR, and the late competence protein ComER. Our data establish the foundation for in-depth study of the regulation of CodY in B. thuringiensis, and also offer a potential biocatalyst for functions of CodY in other bacteria.

• 국제학술발표논문집
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• The 9th International Conference on Construction Engineering and Project Management
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• pp.1242-1242
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• 2022

Annually, several construction workers fall ill, are injured, or die due to heat-related exposure. The prevalence of work-related heat illness may rise and become an issue for workers operating in temperate climates, given the increase in frequency and intensity of heatwaves in the US. An increase in temperature negatively impacts physical exertion levels and mental state, thereby increasing the potential of accidents on the job site. To reduce the impact of heat stress on workers, it is critical to develop and implement measures for monitoring physical exertion levels and mental state in hot conditions. For this, limited studies have evaluated the utility of wearable biosensors in measuring physical exertion and mental workload in hot conditions. In addition, most studies focus solely on male participants, with little to no reference to female workers who may be exposed to greater heat stress risk. Therefore, this study aims to develop a process for objective and continuous assessment of worker physical exertion and mental workload using wearable biosensors. Physiological data were collected from eight (four male and four female) participants performing a simulated drilling task at 92oF and about 50% humidity level. After removing signal artifacts from the data using multiple filtering processes, the data was compared to a perceived muscle exertion scale and mental workload scale. Results indicate that biosensors' features can effectively detect the change in worker physical and mental state in hot conditions. Therefore, wearable biosensors provide a feasible and effective opportunity to continuously assess worker physical exertion and mental workload.

• BMB Reports
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• 제46권2호
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• pp.113-118
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• 2013

TTRAP is a multi-functional protein that is involved in multiple aspects of cellular functions including cell proliferation, apoptosis and the repair of DNA damage. Here, we demonstrated that the lentivirus-mediated overexpression of TTRAP significantly inhibited cell growth and induced apoptosis in osteosarcoma cells. The ectopic TTRAP suppressed the growth and colony formation capacity of two osteosarcoma cell lines, U2OS and Saos-2. Cell apoptosis was induced in U2OS cells and the cell cycle was arrested at G2/M phase in Saos-2 cells. Exogenous expression of TTRAP in serum-starved U2OS and Saos-2 cells induced an increase in caspase-3/-7 activity and a decrease in cyclin B1 expression. In comparison with wild-type TTRAP, mutations in the 5'-tyrosyl-DNA phosphodiesterase activity of TTRAP, in particular $TTRAP^{E152A}$, showed decreased inhibitory activity on cell growth. These results may aid in clarifying the physiological functions of TTRAP, especially its roles in the regulation of cell growth and tumorigenesis.