• 한국감성과학회:학술대회논문집
• /
• 한국감성과학회 1999년도 추계학술대회 논문집
• /
• pp.38-42
• /
• 1999

문명의 발달과 함께 인간은 사회생활의 증가와 부족한 수면으로 인한 스트레스와 병이 증가하고 있다. 따라서 수면에 대한 관심이 증가하면서 편안하고 쾌적한 수면을 위한 수면환경에 대한 연구가 진행되어지고 있다. 본 연구는 쾌적한 온열환경 제시를 위한 방법으로서 여름철 실내환경이 수면에 미치는 영향을 알아보기 위해 22$^{\circ}C$, 26$^{\circ}C$, 3$0^{\circ}C$의 3가지 온도조건을 제시하고 5명의 피험자를 대상으로 수면다원검사를 실시하여 EEG, EOG, ECG, EMG 등의 생리신호를 측정하였다. 측정된 생리신호를 통해 수면단계분석과 수면효율을 분석한 결과 총 수면시간, SWS latency, 총 수면시간에 대한 SWS 시간의 비율이 26$^{\circ}C$의 조건에서 가장 좋은 결과를 나타내었으며, 22$^{\circ}C$, 3$0^{\circ}C$의 순서로 나타났다. 이러한 분석을 통해 온도차에 따라 수면상태가 달라짐을 관찰할 수 있었고, 여름철에 26$^{\circ}C$ 정도의 실내온도가 편안하고 쾌적한 수면을 위한 실내온열환경임을 알 수 있었다.

• 한국정밀공학회지
• /
• 제27권6호
• /
• pp.82-89
• /
• 2010

Ankle-foot orthosis with an artificial pneumatic muscle which is intended for the assistance of plantarfelxion torque was developed. In this study, power pattern of the device in the various pneumatics and the effectiveness of the system were investigated. The pneumatic power was provided by ankle-foot orthosis controlled by user‘s physiological signal, that is, muscular stiffness in soleus muscle. This pneumatic power can assist plantarflexion torque of ankle joint. The subjects performed maximal voluntary isokinetic plantarflexion motion on a biodexdynamometer in different pneumatics, and they completed three conditions: 1) without wearing the orthosis, 2) wearing the orthosis with artificial muscles turned off, 3) wearing the orthosis activated under muscular stiffness control. Through these experiments, we confirmed the effectiveness of the orthosis and muscular stiffness control using the analyzing isokinetic plantarflexion torque. The experimental results showed that isokinetic torques of plantarflexion motion of the ankle joints gradually increased in incremental pneumatic. The effectiveness of the orthosis was -7.26% and the effectiveness of the muscular stiffness control was 17.83% in normalized isokinetic plantarflexion torque. Subjects generated the less isokinetic torques of the ankle joints in wearing the orthosis with artificial muscles turned off, but isokinetic torques were appropriately reinforced in condition of wearing the orthosis activated under muscular stiffness control(17.83%) compared to wearing the orthosis(-7.26%). Therefore, we respect that developed powered orthosis is applied in the elderly that has weak muscular power as the rehabilitation equipment.

• 동의생리병리학회지
• /
• 제20권6호
• /
• pp.1664-1671
• /
• 2006

Cisplatin is a anti-neoplastic agent which is commonly used for the treatment of solid tumor. Cisplatin activates multiple signal transduction pathways involved in the stress-induced apoptosis in a variety of cell types. Cytotoxicity of cisplatin was detected in rat mesangial cells and the value of $IC_{50}$ is about 20 ${\mu}M$. The treatment of cisplatin to rat mesangial cells showed the apoptotic bodies and DNA fragmentation. The activation of caspase-3, -8, and -9 and proteolytic cleavage of PARP were observed in the rat mesangial cells treated time-dependently with cisplatin. The activation of ERK, p38 and JNK was also observed in the apoptosis induced by cisplatin in rat mesangial cells. The ethanol extract of Bojungbangam-tang (EBJT), a new hergal prescription composed of nine crude drugs, inhibited cisplatin-induced apoptosis in rat mesangial cells. EBJT reduced sub-G1 peak (apoptotic peak) in cisplatin-treated rat mesangial cells. The cisplatin-induced ERK and JNK activation in rat mesangial cells were blocked by EBJT, but EBJT had no effect on p38 activation. Taken together, these results con suggest that EBJT prevents cisplatin-induced apoptotic cell death in rat mesangial cells through inhibition of ERK and JNK activation.

• 동의생리병리학회지
• /
• 제30권3호
• /
• pp.150-156
• /
• 2016

Extract of Broussometia kazinoki Rhizodermatis has been traditionally used for geopoong, diuresis, hwalhyeol. In the present study, the apoptotic effect of methanol extract of Broussometia kazinoki (MBK) were investigated. Cell viability of A549 cells was measured by MTT assay. Apoptosis-related protein and MAPK protein levels were measured by Western blot. Chromatin condensation of A549 cells was stained with DAPI. MBK inhibited cell proliferation of A549 cell. Based on DAPI staining, MBK-treated cells manifested nuclear shrinkage, condensation and fragmentation. Treatment of A549 cells with MBK resulted in activation of the caspase-3, -8, -9 and cleavage of poly ADP-ribose polymerase (PARP). In the upstream, MBK increased the expressions Bax and Bak, decreased the expression of Bcl-2, and augmented the Bax/Bcl-2 ratio. MBK-induced apoptosis was accompanied by sustained phosphorylation of JNK, p38 MAPK and apoptosis signal-regulating kinase (ASK)-1. These results suggest that MBK induced apoptosis in A549 cells through Bcl-2 family protein-mediated mitochondria/caspase-3 dependent pathway. In addition, MBK increased the activation of ASK-1, which are critical upsteam signals for JNK/p38 MAPK activation in A549 cancer cells.

• 해양환경안전학회지
• /
• 제23권6호
• /
• pp.684-689
• /
• 2017

본 연구는 참굴(Crassostrea gigas)의 패각운동을 이용하여, 연안역에서 발생하는 저염수에 대한 조기경보가능성을 살펴보았다. 30 psu와 20 psu에서 패각운동은 각각 $7.32{\pm}3.21$회/hr와 $7.11{\pm}3.90$회/hr였으며, 파형과 횟수는 차이가 없었다(t-test, p>0.001). 하지만 10 psu와 5 psu에서는 모든 개체가 폐각상태를 지속하였다. 수온과 염분의 복합실험결과, Group 1(수온 $15^{\circ}C$ ${\times}$ 염분 15 psu)은 20~30 psu에서 보인 패각운동 후(약 2~3시간), 장시간 폐각을 하였다. Group 2(수온 $30^{\circ}C$ ${\times}$ 염분 15 psu)에서는 Group 1의 패각 개폐운동보다 더 빠르고 자주 나타나, 참굴의 생리적인 위기상황에 대한 신호를 나타내었다. 따라서 이러한 파형은 하계 저염수 출현 시 나타낼 수 있는 조기경보 신호로 충분히 활용될 수 있을 것으로 보인다.

• Journal of Plant Biotechnology
• /
• 제48권3호
• /
• pp.148-155
• /
• 2021

Gibberellins (GAs) are essential phytohormones for plant growth that influence developmental processes and crop yields. Recent functional genomic analyses of model plants have yielded good characterizations of the canonical GA signaling pathways and related genes. Although Panax ginseng has long been considered to have economic and medicinal importance, functional genomic studies of the GA signaling pathways in this crucial perennial herb plant have been rarely conducted. Here, we identified and performed functional analysis of the GA signaling-related genes, including PgGID1s, PgSLY1s, and PgRGAs. We confirmed that the physiological role of GA signaling components in P. ginseng was evolutionarily conserved. In addition, the important functional domains and amino acid residues for protein interactions among active GA, GID1, SCF^SLY1, and RGA were also functionally conserved. Prediction and comparison of crystallographic structural similarities between PgGID1s and AtGID1a supported their function as GA receptors. Moreover, the subcellular localization and GA-dependent promotion of DELLA degradation in P. ginseng was similar to the canonical GA signaling pathways in other plants. Finally, we found that overexpression of PgRGA2 and PgSLY1-1 was sufficient to complement the GA-related phenotypes of atgid1a/c double- and rga quintuple-mutants, respectively. This critical information for these GA signaling genes has the potential to facilitate future genetic engineering and breeding of P. ginseng for increased crop yield and production of useful substances.

• 한국군사과학기술학회지
• /
• 제22권2호
• /
• pp.287-297
• /
• 2019

Monitoring for the physiological state of a solider is essential to the realization of individual combat system. Despite all efforts over the last decades, there is no report to point out the optimal location of the wearable biosensors considering both monitoring accuracy and operational robustness. In response, we quantitatively measure body temperature and heartrate from 34 body parts using 2 kinds of biosensor arrays, each of which consists of a thermocouple(TC) sensor and either a photoplethysmography(PPG) sensor or an electrocardiography(ECG) sensor. The optimal location is determined by scoring each body part in terms of signal intensity, convenience in use, placement durability, and activity impedance. The measurement leads to finding the optimal location of wearable biosensor arrays. Thumb and chest are identified as best body parts for TC/PPG sensors and TC/ECG sensors, respectively. The findings will contribute to the successful development of individual combat system.

• 동의생리병리학회지
• /
• 제36권1호
• /
• pp.1-6
• /
• 2022

The tuber of Pinellia ternata (Thunb.) Brei (TPT) used in traditional Oriental medicine for the treatment of cough, sputum, vomiting, and insomnia, possesses antioxidant, antibacterial, and anti-inflammatory effects. Although recent studies have reported the anticancer effects of TPT in several cancer cells, it is still unclear whether TPT regulates tumor-associated macrophage (TAM) characterized by the immunosuppressive M2 macrophage phenotype. Our results showed that the ethanol extract of TPT (ETPT) suppressed the migration of RAW264.7 mouse macrophage cells and THP-1 human monocytes differentiated into macrophages towards the conditioned media (CM) collected from lung cancer cells, suggesting that ETPT would attenuate the recruitment of macrophages into tumors. In addition, ETPT suppressed the interleukin (IL)-4 or IL-6-induced M2 macrophage polarization in RAW264.7 cells. ETPT treatment not only downregulated the mRNA expression of M2 macrophage markers including arginase-1, mannose receptor C type 1 (MRC-1), and IL-10, but also inhibited the phosphorylation of signal transducer and activator of transcription 3 (STAT3) and STAT6, general regulators of M2 macrophage polarization. Finally, the transwell assay results showed that the CM from M2-polarized RAW264.7 cells increased the migration of mouse lewis lung carcinoma (LLC) cells, while those from RAW264.7 cells co-treated with ETPT and IL-6 significantly reduced the migration of LLC cells. Taken together, our observations clearly demonstrate that ETPT suppressed the cancer cell migration by regulating macrophage recruitment and M2 macrophage polarization.

• Journal of information and communication convergence engineering
• /
• 제20권3호
• /
• pp.174-180
• /
• 2022

Emotion recognition is an essential component of complete interaction between human and machine. The issues related to emotion recognition are a result of the different types of emotions expressed in several forms such as visual, sound, and physiological signal. Recent advancements in the field show that combined modalities, such as visual, voice and electroencephalography signals, lead to better result compared to the use of single modalities separately. Previous studies have explored the use of multiple modalities for accurate predictions of emotion; however the number of studies regarding real-time implementation is limited because of the difficulty in simultaneously implementing multiple modalities of emotion recognition. In this study, we proposed an emotion recognition system for real-time emotion recognition implementation. Our model was built with a multithreading block that enables the implementation of each modality using separate threads for continuous synchronization. First, we separately achieved emotion recognition for each modality before enabling the use of the multithreaded system. To verify the correctness of the results, we compared the performance accuracy of unimodal and multimodal emotion recognitions in real-time. The experimental results showed real-time user emotion recognition of the proposed model. In addition, the effectiveness of the multimodalities for emotion recognition was observed. Our multimodal model was able to obtain an accuracy of 80.1% as compared to the unimodality, which obtained accuracies of 70.9, 54.3, and 63.1%.

• Journal of Veterinary Science
• /
• 제23권5호
• /
• pp.72.1-72.14
• /
• 2022

Background: The treatment of acute kidney injury (AKI), a common disease in dogs, is limited. Therefore, an effective method to prevent AKI in veterinary clinics is particularly crucial. Objectives: Hydrogen sulfide (H₂S) is the third gaseous signal molecule involved in various physiological functions of the body. The present study investigated the effect of H₂S on cisplatin-induced AKI and the involved mechanisms in dogs. Methods: Cisplatin-injected dogs developed AKI symptoms as indicated by renal dysfunction and pathological changes. In the H₂S-treated group, 50 mM sodium hydrosulfide (NaHS) solution was injected at 1 mg/kg/h for 30 min before cisplatin injection. After 72 h, tissue and blood samples were collected immediately. We performed biochemical tests, optical microscopy studies, analysis with test kits, quantitative reverse-transcription polymerase chain reaction, and western blot analysis. Results: The study results demonstrated that cisplatin injection increased necroptosis and regulated the corresponding protein expression of receptor interacting protein kinase (RIPK) 1, RIPK3, and poly ADP-ribose polymerase 1; furthermore, it activated the expressions of inflammatory factors, including tumor necrosis factor-alpha, nuclear factor kappa B, and interleukin-1β, in canine kidney tissues. Moreover, cisplatin triggered oxidative stress and affected energy metabolism. Conversely, an injection of NaHS solution considerably reduced the aforementioned changes. Conclusions: In conclusion, H₂S protects the kidney from cisplatin-induced AKI through the mitigation of necroptosis and inflammation. These findings provide new and valuable clues for the treatment of canine AKI and are of great significance for AKI prevention in veterinary clinics.