• BMB Reports
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• v.56 no.6
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• pp.335-340
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• 2023

During normal physiological and abnormal pathophysiological conditions, all cells release membrane vesicles, termed extracellular vesicles (EVs). Growing evidence has revealed that EVs act as important messengers in intercellular communication. EVs play emerging roles in cellular responses and the modulation of immune responses during virus infection. EVs contribute to triggering antiviral responses to restrict virus infection and replication. Conversely, the role of EVs in the facilitation of virus spread and pathogenesis has been widely documented. Depending on the cell of origin, EVs carry effector functions from one cell to the other by horizontal transfer of their bioactive cargoes, including DNA, RNA, proteins, lipids, and metabolites. The diverse constituents of EVs can reflect the altered states of cells or tissues during virus infection, thereby offering a diagnostic readout. The exchanges of cellular and/or viral components by EVs can inform the therapeutic potential of EVs for infectious diseases. This review discusses recent advances of EVs to explore the complex roles of EVs during virus infection and their therapeutic potential, focusing on HIV-1.

• Korean Journal of Remote Sensing
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• v.36 no.5_1
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• pp.667-677
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• 2020

Crop water stress can be detected based on soil moisture content, crop physiological characteristics and remote-sensing technology. The detection of crop water stress is an important issue for the accurate assessment of yield decline. The crop water stress index (CWSI) has been introduced based on the difference between leaf and air temperature. In this paper, drone-based thermal infrared image was used to map of crop water stress in water control plot (WCP) and water deficit plot (WDP) over spring chinese cabbage fields. The spatial distribution map of CWSI was in strong agreement with the abnormal growth response factors (plant height, plant diameter, and measured value by chlorophyll meter). From these results, CWSI can be used as a good method for evaluation of crop abnormal growth monitoring.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.3
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• pp.715-722
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• 2009

The aim of this study was to investigate the safety of short-term use of Korean herbal medicine (KHM; prescribed herbal medicine by doctors of traditional Korean medicine) on liver function. Three hundred eighty four outpatients who took KHM for various conditions were enrolled for multi-center, prospective observational study. Of them 237 patients completed questionnaire and were checked liver function (aspartic aminotransferase;AST, alanine aminotransferase;ALT, alkaline phosphatase;ALP, total bilirubin;t-Bil, direct bilirubin;d-Bil, gamma glutamyltranspeptidase;GGT, protein and albumin) before and after taking KHM (duration; 21.4${\pm}$10.0 days), and data were analysed statistically. Of the 213 patients showing normal liver function test (LFT) at baseline, 209 (98.1%) remained within the normal range at the second test while 3 (1.4%) revealed slight increase of LFT. Only one subject had raised LFT regarding level of liver injury without perceived symptoms. Twenty-four of 237 patients were abnormal at baseline, and 16 at the second testing. Of the patients taking KHM, only 4 changed from normal to abnormal while 12 from abnormal to normal in their LFT. There were no significant increase in LFT level between the first and second test, except in the t-Bil level, however, the change of t-Bil was small and within normal range. The current study showed that the use of KHM did not increase the frequency of abnormal LFTs, at least in the short term.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.3
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• pp.390-400
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• 2010

This study is aimed to test physiological and hematological actions of Rheum palmatum through clinical pilot study optimized for usual oriental medicine prescription. Thirty-one cases were finally collected and the sample extract 100 $m{\ell}$ of Rheum palmatum (ERP) was administered two times in a day during 3 days and checked with blood CBC test, urinalysis, liver function test, abdominal X-ray as well as general diagnostic process of oriental medicine; pattern identification, assessment of shapes and constitution. The total toxic effective rate of ERP was 9.68% in 3 cases of the whole in case of consistent uneasy state in the subjective symptomatic assessment and aggravation of blood and urine examination. The relevant diagnostic factors were so-eum constitution and essence type subject, carapaces species, lung type in the aspect of Jisan shape theory. On the other hand, the positive effective rate of ERP was 29.03% manifesting improvement of vital signs or present illness and no abnormal changes of blood chemistry and urinalysis and simple abdomen radiology. The relevant diagnostic factors were tae-eum constitution, energy type subject, aves species, heart and liver type in Jisan's shape theory. And the other cases were manifesting no specific change through the administration of ERP. The ratio of the numbers of decrease and increase was 44:5 in the tenderness and hardness of abdominal palpation. But if the pattern identification doesn't comply with the aim of rhubarb application despite of constipation and abdominal pain, the tendeness and biochemical report was shown in abnormal change. The mean number of diarrhea by ERP was $16.77{\pm}6.95$ during 3 days after administration, and the frequent areas of abdominal pain were lower>middle>upper in order, and it meant the target site of ERP too. Besides, the toxic reaction against ERP was expressed highly in case of decrease in blood cell count and hemoglobin, hematocrit having blood deficiency syndrome. Likewise, the toxicity of ERP was influenced by pattern identification manifesting present disease condition and diagnostic factors of four constitutions, Jisan's shape theory collaterally. In conclusion, evaluation of herbal toxicity in order for using as a clinical guideline, various diagnostic pattern information and shape features like the above should be studied together with other pharmacologic toxicology test for the future.

• Investigative Magnetic Resonance Imaging
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• v.18 no.3
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• pp.244-252
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• 2014

Purpose : We proposed a multi-physiological signals based real-time intelligent triggering system(MITS) for Cardiac MRI. Induced noise of the system was analyzed. Materials and Methods: MITS makes cardiac MR imaging sequence synchronize to the cardiac motion using ECG, respiratory signal and second order derivative of $SPO_2$signal. Abnormal peaks due to arrhythmia or subject's motion are rejected using the average R-R intervals and R-peak values. Induced eddy currents by gradients switching in cardiac MR imaging are analyzed. The induced eddy currents were removed by hardware and software filters. Results: Cardiac MR images that synchronized to the cardiac and respiratory motion are acquired using MITS successfully without artifacts caused by induced eddy currents of gradient switching or subject's motion or arrhythmia. We showed that the second order derivative of the $SPO_2$ signal can be used as a complement to the ECG signals. Conclusion: The proposed system performs cardiac and respiratory gating with multi-physiological signals in real time. During the cardiac gating, induced noise caused by eddy currents is removed. False triggers due to subject's motion or arrhythmia are rejected. The cardiac MR imaging with free breathing is obtained using MITS.

• Horticultural Science & Technology
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• v.17 no.6
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• pp.737-741
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• 1999

We surveyed the physiological disorders of fruit vegetables grown in the greenhouse in Kyungbuk province in 1998. Greenhouses used for cultivation were mostly single or multi-span iron pipe houses covered with polyethylene film. Single span greenhouses were used for strawberry, oriental melon and watermelon. Fifty six percent of the surveyed farms was a mono-cropping system for oriental melon and tomato. There were greenhouses used for successive cultivation for 10 years or more for strawberry and oriental melon in Koryeong and Seongju. Varieties of fruit vegetables cultivated were diverse, especially in cucumber and watermelon. In strawberry, malformed fruits were observed most frequently in March and the small fruits at late harvest period. Leaf chlorosis, stunt plants and runner outbreak were also found during the growing season. In tomato, occurrence of malformed fruits was severe from March to May, and occurrence of cracked fruits and blossom- end rot was also severe in October and November. The self topping and abnormal stem in tomato were problem in hydroponic cultures in August and November, respectively. Malformed cucumber fruits, such as curved, club shaped, irregular shaped and narrow necked, occurred at late season. Umbrella-shaped leaf in cucumber in summer were caused by calcium deficiency. Most serious disorders were fermented and malformed fruits occurring from March to May in oriental melon, and cracked fruits occurring from April to May in watermelon. At late growing stage of melons the leaf chlorosis occurred with complex symptoms of leaf disease. Growers had little knowledge on physiological disorders, and also on diagnose and measures to cure the disorders. Most growers pointed out that poor soil environment and temperature management in the greenhouse as the main causes of physiological disorders.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.5
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• pp.1035-1040
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• 2009

In this work, we investigate the effect of blood hematocrit level on the radial pulse wave to study the clinical application of the pulse analyzer. For this purpose, we measured the radial pulse wave at the left Gwan for 15 males with abnormal high hematocrit level and 47 males with normal hematocrit level at the age of thirties and forties. Various variables of the radial pulse wave between two groups were analyzed by Student's T test. We found significant differences in several characteristic variables in the amplitude, time-span and the integrated area of the amplitude and time of the pulse wave. The systolic peak in the amplitude of the radial pulse wave was higher in abnormal high hematocrit group. In contrast, the third peak from the second incisure was higher and longer in normal hematocrit group. Our study suggests that the radial pulse wave can be useful in distinguishing the patient group with high hematocrit level and thus with high blood viscosity. Our finding may motivate research activities towards diverse clinical applications of the pulse wave.

• Journal of the Korea Society of Computer and Information
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• v.20 no.4
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• pp.69-76
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• 2015

We present microscopy based hyperspectral imaging system that successively shows high spatial (micrometer) and temporal resolutions (milisecond), and acquired pseudocolor hemoglobin saturation map a result of various image processing techniques can provide additional information such as oxygen transport, abnormal vascularity and therapeutic effects besides structural and physiological measurements in various diseases. To increase understanding of vascular defects several optical methods of imaging for preclinical/clinical assessment have been developed so far. However, they have some limitations for outcoming resolution and user satisfaction level compared to its cost. A hyperspectral imaging system has shown a wide range of vascular characteristics associated with hypervascularity, aberrant angiogenesis or abnormal vascular remodeling in many diseases. This vascular characteristic is considered as a key component to diagnose and detect a type of disease as evidenced by them.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.174-174
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• 2017

To cope with phosphate (Pi) deficient stress, plants modulate various physiological and developmental processes, such as gene expression, Pi uptake and translocation, and root architecture changes. Here, we report the identification and characterization of novel activation-tagged mutant involved in Pi starvation signaling in Arabidopsis. The hpd (${\underline{h}ypersensitive}$ to ${\underline{P}i}$ $ {\underline{d}eficiency}$) mutant exhibits enhanced phosphate uptake and altered root architectural change under Pi starvation compared to wild type. Expression analysis of auxin-responsive DR5::GUS reporter gene in hpd mutant indicated that auxin translocation in roots under Pi starvation are suppressed in hpd mutant plants. Impaired auxin translocation in roots of hpd mutant was attributable to abnormal root architecture changes in Pi starvation conditions. Our results indicated that abnormal auxin translocation in hpd mutant might be due to mis-regulation of auxin efflux carrier proteins, PIN-FORMED (PIN) 1, and 2 under Pi starvation conditions. Not only expression levels but also expression domains of PIN proteins were altered in hpd mutant in response to Pi starvation. Molecular genetic analysis of hpd mutant revealed that the mutant phenotype is caused by the lesion in ENHANCED SILENCING PHENOTYPE4 (ESP4) gene whose function is proposed in mRNA 3'-end processing. The results suggest that mRNA processing plays crucial roles in Pi homeostasis as well as developmental reprograming in response to Pi deprivation in Arabidopsis.

• Journal of Environmental Science International
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• v.24 no.6
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• pp.769-776
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• 2015

This study was conducted to determine the effects of high temperature and deficit irrigation on growth and yield of hot pepper. Hot pepper was subjected to four irrigation treatments: fully irrigation (FI), 10, 20, and 30 days deficit irrigation (DI) combination with high temperature treatment. Control plants were grown natural environment and conventional culture methods. The plant height treated with high temperature was significantly higher than that of control plant. At FI combination with high temperature treatment, growth parameters such as stem diameter, leaf area, fresh and dry weight were the greatest. The yield was the greatest (2,036 kg/10a) under control, DI combination with high temperature treatment decreased by approximately 42% compare with FI combination with high temperature treatment. The number of abnormal fruits was approximately 38/plant under control, which was the smallest and that of 30 days DI combination with high temperature was higher 3.3 times compare with control. Flower abscission and calcium deficiency induced by DI treatments, especially those physiological disorder promoted by increasing DI treatments period. Results indicated that yield of hot pepper reduced by DI treatments, these results suggest that the growers should irrigate to proper soil moisture for preventing reduction of total fruit yield.