• Asian-Australasian Journal of Animal Sciences
• /
• 제11권5호
• /
• pp.498-502
• /
• 1998

The present study was designed, fIrst to develop the new methodology to measure the bioluminescence activity easily in live developing bovine embryos by photoncounting, and secondly to compare the expression efficiency of four luciferase reporter genes in bovine embryos at four- to 16-cell stages. In experiment 1, equimolar pSVlacZ and pSVEluc were microinjected into the pronucleus of fertilized bovine oocytes. At 2 days after micro injection, bioluminescence activity of these embryos was measured by photoncounting with a luminometer for 1 min, and lacZ gene expression in the same embryos was assayed by X-gal staining. All the luciferase-positive oocytes showed some bacterial ${\beta}$-galactosidase activity irrespective of the intensity. In experiment 2, four firefly luciferase genes (pTKEluc, pTK6WEluc, pSVEluc and pMiwluc) were introduced by micro injection, and the injected embryos were cultured for the following 2 days. Detection of the luciferase gene expression was done by photoncounting at 5 to 55 min. Over the measurement period, the luciferase activity was almost constant irrespective of the transgenes microinjected. The luciferase activity and expression efficiency at 2 days after microinjection were not significantly affected by the difference in the microinjected transgenes. The present results demonstrated that the bioluminescence activity in live developing bovine embryos could be measured quickly by photoncounting.

• 대한원격탐사학회지
• /
• 제30권2호
• /
• pp.251-258
• /
• 2014

본 연구는 에어로졸의 광학적 특성 분석을 위한 라이다 시스템의 정확성 향상을 위해 새롭게 개발한 신호 수신 시스템을 설명하고자 한다. 광주과학기술원의 라이다에 아날로그 방식과 광자계수 방식을 통합한 시스템을 활용하여 에어로졸 후방산란 신호를 동시에 관측 가능한 수신단을 개발하였다. 관측된 두 신호 결합을 위해 접합 알고리즘을 고안하였고, 신호 결합에 앞서 광자계수 방식 신호의 Pile up효과를 보정하기 위해 부동시간(Dead time)을 계산하여 보정하였다. 관측 신호 분석을 통해 아날로그 방식 신호, 광자계수 방식 신호, 접합신호 그리고 부동시간 보정에 따른 차이점을 설명하고, 최종적으로 에어로졸 후방산란계수를 산출하여 상호 비교 및 정확성 향상을 확인하였다.