• Proceedings of the KIPE Conference
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• 2014.07a
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• pp.173-174
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• 2014

본 논문에서는 저 대기전력 구현이 가능하며 정확한 출력전압 제어가 가능한 SSR(Secondary Side Regulator) 플라이백 컨버터를 제안하였다. 제안 SSR 플라이백 컨버터는 2차 측에 control IC를 사용하여 별도의 제어기(TL431) 및 포토커플러를 제거하여 구조가 간단하고 대기모드 시 TL431의 바이어스 전류에 의한 전력소모를 줄일 수 있으므로 대기전력을 최소화 할 수 있으며 출력전압을 직접 검출하여 정확하게 출력을 제어할 수 있다. 한편 1차 측의 위치한 게이트 구동을 위해 절연된 1-2차 측간 신호를 전송하는 PET(Pulse Edge Transmitter)를 제안하였으며 제안 방식은 IC로의 직접화가 매우 용이하여 1-2차 측 IC와 제안 PET를 one-chip화 할 수 있다. 제안 회로의 타당성 검증을 위해 10W급 Adaptor의 시작품을 제작하였고, 이를 이용한 실험결과를 바탕으로 제안 시스템의 타당성을 검증한다.

• Nutrition Research and Practice
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• v.10 no.4
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• pp.371-376
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• 2016

BACKGROUND/OBJECTIVES: Chronic ultraviolet (UV) exposure-induced reactive oxygen species (ROS) are commonly involved in the pathogenesis of skin damage by activating the metalloproteinases (MMP) that break down type I collagen. Adenophora remotiflora (AR) is a perennial wild plant that inhabits Korea, China, and Japan. The present study investigated the protective effects of AR against UVB-induced photo-damage in keratinocytes. MATERIALS/METHODS: An in vitro cell-free system was used to examine the scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and nitric oxide (NO). The effect of AR on ROS formation, antioxidant enzymes, elastase, MMP-1 level, and mRNA expression of MMP-1 were determined in UVB-irradiated human keratinocyte HaCaT cells. RESULTS: AR demonstrated strong DPPH free radical and NO scavenging activity in a cell-free system exhibiting $IC_{50}$ values of 1.88 mg/mL and 6.77 mg/mL, respectively. AR pretreatment dose-dependently attenuated the production of UVB-induced intracellular ROS, and antioxidant enzymes (catalase and superoxide dismutase) were enhanced in HaCaT cells. Furthermore, pretreatment of AR prevented UVB-induced elastase and collagen degradation by inhibiting the MMP-1 protein level and mRNA expression. Accordingly, AR treatment elevated collagen content in UVB-irradiated HaCaT cells. CONCLUSION: The present study provides the first evidence of AR inhibiting UVB-induced ROS production and induction of MMP-1 as a result of augmentation of antioxidative activity in HaCaT human keratinocytes. These results suggest that AR might act as an effective inhibitor of UVB-modulated signaling pathways and might serve as a photo-protective agent.

• Proceedings of the KIEE Conference
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• 2005.11a
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• pp.125-129
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• 2005

Mobile display는 사용자가 이동하면서 화면을 보기 때문에 주변 luminance의 변화에 따라서 contrast ratio 변화가 심하다. 특히 햇빛 아래에서는 Mobile display의 화면을 알아보기 어렵다. 본 연구에서는 주변 조도가 증가할수록 TFT LCD의 black 휘도의 변화율이 white 휘도 변화율보다 크게 작용하여 전체 contrast ratio(이하 CR)가 크게 감소함올 확인하였다. 또한, 반투과형 LCD는 반사창을 통해서 외부 빛올 반사시키기 때문에 주변 조도가 높아도 일정 수준의 CR은 유지할 수 있다. 그러나 투과형 LCD보다 CR이 낮았다. 본 논문에서는 투과형 TFT LCD에 back light 광원으로 LED를 사용하고, photo diode와 LED drive IC를 이용하여 주변 조도의 광량에 따라서 LCD의 back light LED의 휘도를 3단계 조절할 수 있는 회로를 구성하였다. 이렇게 함으로써 주변 조도에 따라 자동으로 back light 휘도 조절이 가능한 결과을 얻었다.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.14 no.6
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• pp.37-44
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• 2005

This paper presents fabrication techniques of the optical fiber position sensor (or spindle state monitoring. These include selection of components such as optical fibers, a laser-diode, a photo-diode, and op-amp IC of the signal process circuit. We also describe electric runout problem. The fabricated sensor has a linearity of $1.7\%$ FSO in the air gap range $0.1\~0.6mm$, a resolution of $0.37{\mu}m$ and a bandwidth of 6.3kHz. Finally, we have successfully operated a magnetic bearing spindle system using the sensors.

• Proceedings of the KIPE Conference
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• 2014.07a
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• pp.277-278
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• 2014

본 논문은 저 대기전력의 구현이 가능하며 출력전압을 정확하게 제어할 수 있는 플라이백 컨버터 제어방식을 제안한다. 제안 방식은 모든 절연 형 컨버터에 적용 가능하고, 2차 측에 PFM(Pulse Frequency Modulation) IC를 위치시켜 정확한 출력전압 제어가 가능하다. 또한, 1,2차 측간에 신호 전달을 위하여 기존에 사용되는 TL431과 포토커플러가 아닌 Y-capacitor와 Low-Pass-Filter를 적용하여, 저 대기전력 구현이 가능하다. 제안방식의 타당성을 검증하기 위하여 이론적 해석과 시뮬레이션을 통하여 가능성을 확인하였다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.1
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• pp.42-46
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• 2010

This study was performed to investigate anti-wrinkle effects of Acanthopanax senticosus (AS) on ultraviolet B (UVB)-induced photoaging with wrinkle formation. AS extract showed higher DPPH radical scavenging activity (3 ${\mu}g/mL$ as $IC_{50}$) and collagenase inhibition (1.52 mg/mL as $IC_{50}$) than those of ascorbic acid (50 ${\mu}g/mL$ and 2.17 mg/mL, respectively). Cell proliferation and type I pN collagen synthesis were increased by 11.4% and 96.4%, respectively, compared with non treatment control. In vivo, SKH-1 hairless mice were administrated AS 400 mg/kg for 10 weeks with UVB irradiation three times a week. After 10 weeks, a visual assessment and replica assay were performed on each mouse. According to visual assessment of close-up photos and skin replica, oral administration of A. senticosus affected on inhibition of wrinkle formation caused by UVB irradiation on the skin of mice as compared to the vehicle treated control mice. These results indicated that A. senticosus could protect skin wrinkle formation caused by collagen synthesis of fibroblast cells and photo-irradiation of UVB in hairless mice.

• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.346-353
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• 2014

The chlorophyll-related compound pheophorbide a (Pa) was successively purified from an edible red seaweed, Grateloupia elliptica, using silica, octadecyl silica column chromatography and reversed phase-high-performance liquid chromatography, as well as the cell cycle inhibitory and apoptotic effects of Pa being investigated in U87MG glioblastoma cells. The Pa exhibited strong anticancer effects in the absence of direct photo-irradiation against various cancer cell lines, including U87MG, SK-OV-3, and HeLa cells. Among the cancer cells, the strongest anticancer activity of Pa exhibited on U87MG cells with $IC_{50}$ values of 2.8 ${\mu}g/ml$. In addition, Pa specifically had cytostatic activity on glioblastoma cells rather than human umbilical vein endothelial cells. Analysis of the cell cycle distribution showed that Pa induced G0/G1 arrest of U87 MG cells. In addition, arrested cells induced late apoptosis and DNA degradation under dark condition. These results suggest that Pa isolated from G. elliptica is a potential glioblastoma-specific anticancer agent without side effects on normal cells.

• Mycobiology
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• v.40 no.2
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• pp.117-123
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• 2012

Nuruk contributes to the unique characteristics of Korean alcoholic beverages. In this study, the effects of nuruk extracts (NE) on anti-oxidant characters, melanogenesis, and anti-photoaging activity were investigated. NEs were obtained from the 70% ethanol extracts of six types of nuruk, which have been used in brewing of fermented alcohol beverages in Korea. First, various antioxidant characteristics were identified in terms of 2,2'-azino-bis(3-ethylbenzthiozoline-6-sulphonic acid) (ABTS) radical scavenging activity, superoxide dismutase (SOD) expression, and inhibition of xanthine oxidase activity. NE#4 exhibited potent ABTS radical scavenging activity ($IC_{50}$ = 19.51 ${\mu}g$/mL). Compared with NE#4, relatively lower levels of activity were observed for NE#3 and NE#6, with $IC_{50}$ values of 90.99 and 76.88 ${\mu}g$/mL, respectively. According to results of western blot analysis for determination of SOD expression in $H_2O_2$-treated HepG2 cells, NE#5 and NE#6 induced a dramatic increase in the expression ratio of SOD, compared to the group treated with $H_2O_2$ only. Activity of xanthine oxidase, which converts xanthine into uric acid, generating superoxide ions, was inhibited by NE#4 and NE#6 in a dose-dependent manner. NE#4 induced significant inhibition of mushroom tyrosinase activity. A reduction in cellular melanin contents of 80% was observed in B16F1 melanocytes treated with NE#5 and NE#6; these effects were similar to those of arbutin at 100 ${\mu}M$. In addition, gelatin zymography and reverse transcription-PCR analysis were performed for assessment of anti-photoaging activity of Nuruk. Treatment with NE#6 resulted in dramatically inhibited activities of matrix metalloproteinase (MMP)-2/-9, suppressed expression of MMP-1, and increased expression of type-1 procollagen. Results of gelatin zymography for NE#4 and NE#5 were similar, to a slightly lesser degree. These results suggest the potential of NE#4 and NE#6 as natural ingredients for use in functional foods and cosmetics.

• Food Science and Preservation
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• v.20 no.5
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• pp.684-690
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• 2013

The antioxidant and photoprotective effects of various extracts from the roots of Rumex crispus L. were evaluated. The concentrations ($IC_{50}$) of various extracts required to exert a 50% reducing effect on a DPPH radical were found to be 0.005~0.093 mg/mL. The ethyl acetate extract showed a more remarkable effect than the positive control ascorbic acid. The concentrations ($QC_{50}$) of the butanol and ethyl acetate extracts required to exert a 50% reducing effect on the singlet oxygen $^1O_2$ were found to be 0.464 and 0.365 mg/mL, respectively. Both extracts were also found to protect the in vitro biological system from the detrimental effect of a singlet oxygen $^1O_2$ on type II photosensitization in E. coli and genomic DNA. Among all the tested extracts, the ethyl acetate and butanol extracts contained higher amounts of total phenolic contents. The results suggest that our study may contribute to the development of new bioactive products with potential applications to the reduction of photo-produced oxidative stress involving reactive oxygen species in living organisms.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.1 s.49
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• pp.103-109
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• 2005

UV irradiation leads to distinct changes in skin connective tissue, which is degradation of collagen. Many of these alterations in the extracellular matrix are mediated by matrix metalloproteinases. In this study, to develop a new anti-aging agent, we screened the antioxidant activity of solvent fractions from ethanolic extract of Melothria Heterophylla. Among the four solvent fractions tested, the EtOAc fraction exhibited the highest antioxidant activity. It was investigated the inhibitory effect of the EtOAc fraction on the expression and activity of MMP-1 in UVA-irradiated human dermal fibroblasts. The EtOAc fraction inhibited the activity of MMP-1 in a dose dependent manner with the $IC_{50}$ values of $9{\mu}g/mL$. Also, UVA-induced MMP-1 expression was reduced about $90\%$ by $100{\mu}g/mL$ of the EtOAc fraction but MMP-1 mRNA expression was not inhibited. Therefore, we conclude that the EtOAc fraction significantly inhibits MMP-1 expression at the protein level. From these results, we suggest that the EtOAc fraction from M. heterophylla could be used as a new anti-aging agent for the photo-damaged skin.