• Microbiology and Biotechnology Letters
• /
• v.33 no.2
• /
• pp.136-141
• /
• 2005

A bench-scale feasibility study was conducted with solid farming feed to evaluate a treatment process for microbiological removal of nitrogen (N) and phosphorus (P). Strains, Bacillus sp. CK-10 and Bacillus sp. CK-13, were originally isolated from water samples of shrimp farming pond. Simultaneous removal of N/P in marine media was monitored in the co-cultures, CK-10 and CK-13. As the results, $400\;{\mu}M\;NH^{+}_4$ and $400\;{\mu}M\;NO^{-}_2$ were eliminated within 12 hours and $NO^{-}_3$ within 36 hours, and $500\;{\mu}M\;PO^{-3}_4$ was completely disappeared within 36 hours from the media. Cultures of CK-10 and CK-13 were applied for removal of N/P leached from shrimp farming fred. HPAEC-PAD system was used to analyze sugars in farming feed, resulting in resolution of various sugars including glucose, galactose, galatosamine, mannose, and fucose. $0.2\%$ (w/v) Pulp densities of the farming feed contained approximately $33.3\;{\mu}M\;NH^{+}_4,\;12.9\;{\mu}M\;NO^{-}_2.\;81.5\;{\mu}M\;NO^{-}_3\;and\;248\;{\mu}M\;PO^{-3}_4$ which could dissolved within 72 hours of leaching in aqueous solution followed by bacterial removal. Complete bacterial removal of N/P was achieved within 84 hours at $0.2\%$ of the feed in co-cultures, whereas single cultures removed to incompletion of N/P during the incubation period. This work demonstrated that test cultures, CK-10 and CK-13 showed effective removal of N/P derived from shrimp farming feed.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.11 no.1
• /
• pp.132-137
• /
• 2003

This study was carried out to investigate removal character of nitrogen and phosphorus with injection time of acetic acid on SBR, which is one of the biological treatment process. Wastewater used in experiment period was swine wastewater with character, relatively lower organic material concentration than nitrogen concentration. In the experiment with injection time of acetic acid, run 1 wasn't injected acetic acid during the anoxic period, and run 2 was injected intermittently acetic acid during the anoxic period of 15 hours. And run 3 was injected intermittently during the anoxic period of 3hours from end of wastewater filling. And filing time of the wastewater was 20hour from run 1 to run 3. In the study, the highest removal efficiency of organic and nitrogen were obtained by the operating condition of Run 2(the ratio of mixing/aeration time : 16.5/5.5, injection time of acetic acid : 15hours) and T-P was obtained by the operation condition of Run 3(the ratio of mixing/aeration time : 16.5/5.5, injection time of acetic acid : 3hours),and removal efficiency of $BOD_5$, $COD_{Mn}$, $COD_{Cr}$, T-N and T-P in the treated water was 96.1%, 87.7%, 90.6%, 86.6% and 84.5%, respectively.

• The Journal of Korean Academy of Prosthodontics
• /
• v.43 no.3
• /
• pp.363-378
• /
• 2005

Statement of problem. Hydroxyapatite(HA) coated titanium surfaces have not yet showed the reliable osseointegration in various conditions. Purpose. This study was aimed to investigate microstructures, chemical composition, and surface roughness of the surface coated by the hydrothermal method and to evaluate the effect of hydrothermal coating on the cell attachment, as well as cell proliferation. Material and Methods. Commercially pure(c.p.) titanium discs were used as substrates. The HA coating on c.p. titanium discs by hydrothermal method was performed in 0.12M HCl solution mixed with HA(group I) and 0.1M NaOH solution mixed with HA(group II). GroupⅠ was heated at 180 $^{\circ}C$ for 24, 48, and 72 hours. GroupⅡ was heated at 180 $^{\circ}C$ for 12, 24, and 36 hours. And the treated surfaces were evaluated by Scanning electron microscopy(SEM), Energy dispersive X-ray spectroscopy(EDS), X-ray photoelectron spectroscopy(XPS), X-ray diffraction method(XRD), Confocal laser scanning microscopy(CLSM). And SEM of fibroblast and 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assay were used for cellular responses of the treated surfaces. Results. The color of surface changed in both groups after the hydrothermal process. SEM images showed that coating pattern was homogeneous in group II, while inhomogeneous in group I. H72 had rosette-like precipitates. The crystalline structure grew gradually in group II, according to extending treatment period. The long needle-like crystals were prominent in N36. Calcium(Ca) and phosphorus(P) were not detected in H24 and H48 in EDS. In all specimens of group II and H72, Ca was found. Ca and P were identified in all treated groups through the analysis of XPS, but they were amorphous. Surface roughness did not increase in both groups after hydrothermal treatment. The values of surface roughness were not significantly different between groups I and II. According to the SEM images of fibroblasts, cell attachments were oriented and spread well in both treated groups, while they were not in the control group. However, no substantial amount of difference was found between groups I and II. Conclusions. In this study during the hydrothermal process procedure, coating characteristics, including the HA precipitates, crystal growth, and crystalline phases, were more satisfactory in NaOH treated group than in HCl treated group. Still, the biological responses of the modified surface by this method were not fully understood for the two tested groups did not differ significantly. Therefore, more continuous research on the relationship between the surface features and cellular responses seems to be in need.

• Journal of Animal Environmental Science
• /
• v.15 no.2
• /
• pp.161-170
• /
• 2009

This experiment was conducted to investigate the effects of compost leachate and concentrated pig slurry on growth of pepper in substrate hyrdoponic culture. In process of composting, compost leachate was was through a saturated compost heap. Pig slurry was filtered by ultra filtration and concentrated by reverse osmosis process. The pig slurry was mixed with chemical nutrient solution and byproduct based on nitrogen content. Peppers were grown in the seven different hydroponic solutions; compost leachate (CL), concentrated pig slurry (CS), compost leachate + byproduct (CL+BP), concentrated pig slurry + byproduct (CS+BP), compost leachate 50% + nutrient solution 50% (CL+NS), concentrated pig slurry 50% + nutrient solution 50% (CS+NS) and chemical nutrient solution for pepper. The chemical nutrient solution was the standard solution of National Horticulture Research Station for the growth of pepper. The concentration of nutrient solution was adjusted $1.6{\sim}2.0mS/cm$ in EC. The compost leachate and concentrated pig slurry were low in phosphorus (P), calcium (Ca), magnesium (Mg), but rich in potassium (K). Growth characteristics as affected by the different nutrient solution were significantly different. Growth and fruit characteristics treated with CL 50+NS 50%CS and 50+NS 50% were similar with NS 100% control plot. The dry weight of stem and leaf were 107.4, 104.2g in plot of NS 100% and CS 50%+NS 50%, respectively. The fruit of pepper showed lowest in the plot treated with 100% concentrated pig slurry, and the growth of pepper severely decreased after application of 100% CS treatment. The yield of pepper was not significantly different between the plots treated with mixture of CS50 + NS50% and 100% nutrient solution treatment. Fruit yield of the compost leachate concentrated pig slurry plot were 59, 14% compared to control, repectively. In conclusion, the mixture solution of 50% of pig slurry and 50% of nutrient solution could be used as a nutrition solution of pepper in hydroponic culture.

• Journal of the Korean GEO-environmental Society
• /
• v.8 no.6
• /
• pp.21-28
• /
• 2007

The objective of this study was to investigate the effect of hydraulic retention time (HRT) on removal efficiencies of organic matter, nitrogen and phosphorus in MBR-RO process for treating synthetic sewage. In MBR process, turbidity was less than average 2 NTU and average removal efficiency showed more than 99% during the operation period(MBR 105 day). As a result of HRT variation, average removal efficiencies of $COD_{Cr}$ on HRT 6, 12, 18 and 24hour were about 72.4, 84, 88.6 and 92.5%, respectively. The $NH_4{^+}-N$ removal efficiency was about 60.2 85.5, 91.3 and 92.2%, respectively. T-N and T-P removal efficiencies increased from 53.7 and 56.8 to 82.5 and 86.4%, respectively as the HRT increased from 6 hour to 24 hour. In RO process, average removal efficiencies of color and $COD_{Cr}$ in RO permeate were about 99.9% and 96.8%, respectively. Also, removal efficiencies of T-N, $NH_4{^+}-N$, $NO_3{^-}-N$ and T-P were all above average 90%.

• Journal of Korean Society of Environmental Engineers
• /
• v.29 no.2
• /
• pp.229-234
• /
• 2007

In the present work, the sequencing batch reactor process of two-sludge type was optimized. The effects of solid retention time, hydraulic retention time, length of biosorption phase and temperature variation were investigated, respectively. In the T-N removal, the long solid retention time was favored. It was speculated that SCOD biosorption efficiency was higher in long solid retention time than in short solid retention time. In the comparison of hydraulic retention time, the removal efficiency of $NH_4^+-N$ and T-N were almost same in all applied hydraulic retention times which were 8 hr, 10 hr and 15 hr. It was observed that there was no need to have the hydraulic retention time more than 20 min in biosorption phase for enhancement of T-N removal efficiency. An experimental comparison of removal efficiencies with different temperature conditions was carried out. Decrease of temperature didn't affect the performance of the process, however, phosphorus removal efficiency was a little higher at low temperature than high temperature. Consequently, the process developed in this study was much amenable to wastewater treatment which was conducted in the low temperature and high loading rate.

• Journal of Korean Society of Environmental Engineers
• /
• v.30 no.10
• /
• pp.992-998
• /
• 2008

Recently, a new concept for nitrogen removal that is simultaneous nitrification and denitrification(SND) has been studied for wastewater treatment process. The DMR(Daiho Microbic Revolution) process that used in this study consists of two suspended anoxic, anaerobic reactors and an aerobic biofilm reactor. The function of aerobic environment and the intensity of air flow rate(2.0, 1.0, 0.5, 0.4, 0.2 L/min) were studied in the biofilm reactor; also SND and nutrient removal efficiencies were investigated. Experimental results indicated that the change in air flow did not affect COD$_{Cr}$ removal significantly. Thus sustained at 93%. The lower the air flow rate, the higher T-N removal efficiency was attained(i.e.80% at 0.2 L/min). SND efficiency was 62, 65, 72 and 78% corresponding to each air flow rate. T-P removal was sensitive to aeration intensity and removal enhanced from 75% to 96% when the air flow rate was changed from 2.0 to 0.5 L/m; however second release occured in the clarifier at 0.2 L/min. Phosphorus content of activated sludge was 5.0%, as P releases and acetate uptake a ratio of 0.75 mg P/ mg HAc.

• Clean Technology
• /
• v.19 no.3
• /
• pp.295-299
• /
• 2013

This study was purposed and performed to evaluate removal efficiency of non-point source pollution in the process and system based on bio-retention design criteria regulated by EPA. Basic Column Reactors (BCR) were prepared for optimal determinations of inflow rate of first rainfall runoff and composition and ratio of soil layers. Removal efficiencies of non-point source pollution from synthetic runoff and real first rainfall runoff, directly sampled from motor way and parking lot, were analyzed, respectively. Removal efficiency of SS, BOD, COD, T-N, and T-P were all shown to be more than 80%.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2002.10a
• /
• pp.1033-1036
• /
• 2002

Wafer bonding methods such as fusion and anodic bonding suffer from high temperature treatment, long processing time, and possible damage to the micro-scale sensor or actuators. In the localized bonding process, beating was conducted locally while the whole wafer is maintained at a relatively low temperature. But previous research of localized heating has some problems, such as non-uniform soldering due to non-uniform heating and micro crack formation on the glass capsule by thermal stress effect. To address this non-uniformity problem, a new heater configuration is being proposed. By keeping several points on the heater strip at calculated and constant potential, more uniform heating, hence more reliable wafer bonding could be achieved. The proposed scheme has been successfully demonstrated, and the result shows that it will be very useful in hermetic packaging. Less than 0.2 ㎫ contact Pressure were used for bonding with 150 ㎃ current input for 50${\mu}{\textrm}{m}$ width, 2${\mu}{\textrm}{m}$ height and 8mm $\times$ 8mm, 5mm$\times$5mm, 3mm $\times$ 3mm sized phosphorus-doped poly-silicon micro heater. The temperature can be raised at the bonding region to 80$0^{\circ}C$, and it was enough to achieve a strong and reliable bonding in 3minutes. The IR camera test results show improved uniformity in heat distribution compared with conventional micro heaters. For gross leak check, IPA (Isopropanol Alcohol) was used. Since IPA has better wetability than water, it can easily penetrate small openings, and is more suitable for gross leak check. The pass ratio of bonded dies was 70%, for conventional localized heating, and 85% for newly developed FP scheme. The bonding strength was more than 30㎫ for FP scheme packaging, which shows that FP scheme can be a good candidate for micro scale hermetic packaging.

• Journal of Korean Society on Water Environment
• /
• v.33 no.6
• /
• pp.722-727
• /
• 2017

Livestock manure is known to be the main cause of non-point pollution in agricultural areas. The pollutant reduction ratio of livestock manure recycling to fertilizers was measured in order to analyze the effect on the water quality of the Total Maximum Daily Load (TMDL) system in Korea. The reduction ratio has been applied by theoretical consideration without a survey, and there is no value for Total Organic Carbon (TOC) newly introducing any organic items. The reduction ratio of each pollutant from this study was revealed as follows: TOC, BOD, T-N and T-P were 0.34, 0.60, 0.37, and 0.42 for individual farm and 0.38, 0.61, 0.45 and 0.44 for entrustment facilities, respectively. The reduction ratio of individual farm was surveyed as TOC 0.63, BOD 0.62, T-N 0.42 and T-P 0.32 for liquid fertilizer, and TOC 0.30, BOD 0.64, T-N 0.40 and T-P 0.48 for compost. The total reduction ratio was derived by multiplying the ratio for liquid fertilizer and compost by the respective load. Compared to the pollutant reduction ratio of the individual farm with entrustment facilities marking the higher in liquid fertilizer and the lower in compost. Through this study, we found the difference of pollutant reduction ratio between a livestock manure recycling process and facilities. Although phosphorus is known as a preservative matter, the treatment efficiency of T-P is analyzed to decrease by chemical precipitation.