• Asian-Australasian Journal of Animal Sciences
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• v.21 no.8
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• pp.1178-1182
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• 2008

The present study was designed to assess the influence of caeca on the availability of calcium, phosphorus, magnesium, manganese and copper from soybean, sunflower, rapeseed, sesame, fish and meat cum bone meal in adult roosters. The excretion of endogenous origin minerals viz., copper, magnesium, manganese and calcium was significantly (p<0.001) higher in caecectomized than in normal roosters. The difference in the endogenous excretion was 50; 60.45; 40.35 and 29.63 per cent for copper, magnesium, manganese and calcium, respectively, in caecectomized roosters. The caeca played a pivotal role in the reabsorption of endogenous origin calcium, magnesium, manganese and copper. The mechanism of phosphorus absorption by the caecal epithelium was negligible. The caecectomized roosters underestimated the bioavailability of copper in sunflower meal and manganese in almost all the test feedstuffs. The present investigation revealed that the caeca played a critical role in the absorption of minerals from vegetable protein feedstuffs which escape digestion and absorption in the small and large intestinal segments.

• Journal of Nutrition and Health
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• v.28 no.11
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• pp.1049-1055
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• 1995

This study was designed to investigate the effects of calcium supplementation on calcium metabolism in seven healthy college women, aged from 19 to 21 years old. For this purpose, metabolic studies were conducted for two weeks. During the first week, the subjects ate experimental diet which nutrients composition was similar to their usual intake. And during the consecutive second week, they ate the same experimental diet supplemented with 500mg of calcium daily. The results were summarized as follows ; 1) Fecal excretion of calcium increased significantly (P<0.05), but urinary excretion of that did not show any change after supplementary intake of calcium. 2) Mean apparent calcium absorption was 28.5% and retention was 182mg/day when subjects ate the experimental diet without calcium supplementation. Calcium retention was significantly ate the experimental diet without calcium supplementation. Calcium retention was decreased to 24.1% by additional intake of calcium. 3) Phosphorus balance did not show any change after additional intake of calcium. 4) Serum calcium level was also not changed by additional intake of calcium. 5) Serum calcium level increased significantly(P<0.05) but serum phosphorus level did not show any change after additional intake of calcium. The above results showed that supplementation of 500mg calcium daily can be helpful to increase calcium retention as well as the peak bone mass in young women eating usual Korean diets.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.4
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• pp.393-402
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• 1995

Experiments were conducted to evaluate the nutritive values of supplemental L-lysine, liquid and powder type, and DL-methionine in weanling pigs. For feeding trial, 165 weanling pigs were treated in 2 controls; 18 and 16% CP, 6 supplementations of lysine alone to 16% CP diets; 0.1, 0.2 and 0.4% of liquid and powder type each, and 3 supplementations of lysine + methionine to 15% CP diets; 0.05 + 0.025, 0.1 + 0.05 and 0.2 + 0.1%. Pigs were fed for 5 week to investigate the protein sparing effect of supplemental amino acid, and the optimal supplemental level. A metabolic trial included the measurements of digestibilities of dry matter, crude protein, crude fat, crude fiber, energy, phosphorus and amino acids. The liver acinar cell culture was conducted for the protein synthesis activity of the pigs fed each experimental diet. Supplementation of both type of L-lysine in 16% CP diet showed improved daily weight gain and feed efficiency which were compatible with those of pigs fed 18% CP diet. Groups fed liquid lysine did not differ from those fed powder type in growth performance. Supplementation of lysine and methionine to 15% CP diet did not improve growth performance of pigs to the extent that 18% CP diet was fed. In nutrient digestibility, 16% CP control diet showed significantly (p < 0.05) lower crude protein digestibility than any other treatments. Digestibilities of 16% CP diets with lysine supplementation were equal to that of 18% CP control, while digestibilities of 15% CP diets with the supplementation of lysine + methionine was inferior to that of 18% CP control. Supplementation of lysine alone reduced the nitrogen excretion compared to the none supplemented control groups. However, addition of lysine + methionine excreted more nitrogen than controls. Pigs fed diet supplemented with lysine alone, or lysine + methionine excreted less fecal phosphorus than those fed none supplemetation. Retained protein from liver tissue of pigs fed 18% diet was significantly (p < 0.05) greater than those fed 16% CP diet. A significant difference (p < 0.05) was observed in physical type of lysine. Feeding of powder type showed less secreted protein and greater retained protein in the culture of liver acinar cell. It is concluded that supplementation of lysine at the level of 0.1 to 0.2% can spare 2% of dietary protein and reduce nitrogen excretion by 19.3%. Also, no difference in nutritional values was observed between liquid and powder lysine in weanling pigs.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.5
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• pp.673-680
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• 2000

An experiment was conducted with day-old 300 commercial male broiler chicks (Arbor Acres$^{(R)}$) to evaluate the efficacy of crude phytase preparerations produced from a culture of Aspergillus ficcum. The experiment consisted of five dietary treatments; T1, com-soy control diet with 0.45% non-phytate phosphorus (NPP) for starter period and 0.35% NPP for grower period; T2, control - 0.1% NPP; T3, control 0.2% NPP; T4, T3+600 U of crude phytase (broth+cell); and T5, T3+600 U of crude phytase (broth). The body weight gain, feed intake, and feed/gain of chickens fed T1 diet was highest (p<0.01) among treatments. BW gain and feed intake of T4 and T5 were greater than those of T3 but were less than those of T1 and T2. T3 was highest in mortality among treatments. Decreasing the NPP level lowered availability of DM, crude ash, ether extract, crude fiber, Zn, and Fe but supplementation of crude phytase preparations improved the availability of these nutrients as well as those of Ca, P and Cu. Excretion of P and Cu significantly decreased as the NPP level in the diet decreased. Further reduction of P and Cu excretion and reduction of Ca, Mg and Fe excretion were achieved by supplementation of crude phytase preparations. The serum concentrations of Ca, P, Mg, Zn, Fe, and Cu were significantly increased by crude phytase supplementation. The weight and length of tibia, and contents of crude ash, Ca, P, Mg, and Zn were adversely affected by lowering NPP level but partially recovered by supplementation of crude phytase preparations. In conclusion, lowering NPP level in the broiler diet significantly depressed the performance. Supplementation of crude phytase preparations produced from Aspergillus ficuum could partially recover the depression.

• Korean Journal of Poultry Science
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• v.27 no.1
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• pp.19-23
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• 2000

The effects of microbial phutase on laying performance and phosphorus utilization were examined at different levels of dietary nonphosphorus(NPP) in 320 23-week-old Hy-line brown hens for 12weeks. Diets were formulated 0.275%(T1), 0.220%(T2), 0.165%(T3) of NPP levels, and supplemental microbial phytase was 300DPU/kg diet constantly. Conventional diet(C) was formulated 0.275% NPP level without microbial phytase. Egg production and egg mass were higher in T2 than the others (p<0.05), and average egg weight was higher in T1 than the other (p<0.05). Egg productivity was tended to increase with supplemental phytase compared to conventional diet. Daily feed intake a hen also increased in T2 (p<0.05). Feed conversion ratio was improved slightly without significant difference. Eggshell breaking strength and thickness were not different significantly among the treatments. Haugh unit and yolk color were also not different. Calcium and phosphorus retention in body increased in T2 (p<0.05), but dry matter and nitrogen retention were not different significantly. Differences in nitrogen and calcium excretions were not found among the treatments. But phosphorus excretion decreased in order of dietary phosphorus levels with supplement phytase compared to C (p<0.05). Tibial ash, calcium and phosphorus were similar among the treatments. In conclusion, supplemental microbial phytase in laying diet may help to utilize phytase phosphorus, and could decrease NPP intake.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.2
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• pp.197-202
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• 1999

Three barrows weighing 45.0 kg, fitted with simple T-cannulas in both the duodenum and terminal ileum, were assigned to diets in a $3{\times}3$ Latin Square design experiment to determine the effect of two calcium levels (0.8% vs 0.4%) on phytase activity and nutrient balance in growing pigs. The control diet contained 0.8% calcium, with no added inorganic phosphorus (0.45% total phosphorus) and no added phytase. The two additional experimental diets contained microbial phytase (750 phytase units/kg) and supplied either 0.8% or 0.4% calcium. With added microbial phytase, ileal and total tract digestibility of rotal phosphorus were improved by 20.9 and 13.8 percentage units, respectively (p=0.01). The apparent duodenal and ileal digestibility of phytate phosphorus were increased by 51.8 and 49.7 percentage units (p=0.01). Lowering dietary calcium in the presence of microbial phytase increased the digestibility of phytate phosphorus by an additional 10.9 (p=0.001) and 5.7 percentage units for duodenal and ileal digestibility, respectively. Supplementation with microbial phytase significantly reduced fecal excretion of nitrogen and phosphorus and increased the percentage of these nutrients retained by the pig. Lowering dietary calcium further increased the percentage of dietary phosphorus retained. Overall, reducing dietary calcium appeared to increase the effectiveness of added microbial phytase in degrading phytate phosphorus. As a result, care should be taken to avoid high levels of dietary calcium when supplementing swine diets with microbial phytase.

• Journal of Animal Environmental Science
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• v.6 no.3
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• pp.141-151
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• 2000

Over-grown seaweeds in Donghae(east sea of Korea peninsula) may impedes ocean environment, however, they can be a good nutrient resources for poultry feeds if they are utilized properly. In this study, seaweeds powder were tested for laying hens\` ration to investigate the effects on egg production rate, egg quality and calcium phosphorus excretion. One hundred 65wks-old brown layers were fed for 5 weeks alotted with seaweeds powder addition to experimental diet by 0(control), 0.5, 1.0 and 2.0%, respectively, and obtained following results; 1. Seaweeds proved a mid-protein low-energy feed resources with planty of K, Na, Ca, Mg, Sr and Fe. 2. Seaweeds addition by 0.5% and 1.0% improved egg production rate and egg-mass output markedly(p<0.05) than control. Seaweeds addition did not alter cholesterol level of yolk and yolk index, however egg shell thickness showed increasing trend by increasing seaweeds addition level. 3. Protein absorption and digestibility in seaweed addition treatments were significantly higher(p<0.05) than control group and protein contents of excreta in 0.5% and 1.0% treatments were reduced(p<0.05), which suggests effective protein metabolism for egg production. Increasing seaweeds addition reduces Ca and P contents in rectum and excreta, suggesting Ca and P utilization improvement in laying hens and lessening soil pollution. 4. In conclusion, seaweeds addition in layers' diet by 1.0% level improves egg-mass production and might be egg quality by increasing metabolism of protein, calcium and phosphorus.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.6
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• pp.1006-1015
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• 1996

This study was carried out to investigate the effects of dietary calcium-salt, estrogen-treatment, and estrogen/calcium treatment on calcium, phosphorus and nitrogen metabolism. Female Sprague-Dawley rats with a body weight of 250~280g were underwent ovariectomy or sham-operation. The ovariectomized rats were divided into 9 different experimental groups including the saline-treated group, the estrogen-treated group, the high calcium salt-treated group, and the estrogen/calcium treated groups and fed for 6 weeks. Each group daily intake and excretion of calcium, phosphorus and nitrogen were measured and apparent digestibility and balance were also studied. The results were as follows: The excretion level of winn calcium was significantly higher the ovariectomized rats than the sham-operation group(p<0.01) and reduced with estrogen treatment but this difference was not statistically significant. Fecal loss of calcium was higher the ovariectomized rats than the sham-operation group(p<0.001). When the estrogen was treated, fecal loss was decreased and then apparent digestibility of calcium was increased. Calcium balance was significantly higher the high calcium treated groups than the control diet groups. The excretion level of urinary Phosphorus was higher the ovariectomized rats than sham-operation group, while these showed to be decreased in the calcium salt, the estrogen and the estrogen/calcium treated groups(p<0.01). Fecal loss of phosphrous was higher in the ovariectomized rats. When the estrogen was treated, the fecal loss was decreased in the avariectomized rat than that of other groups. The excretion level of urinary nitrogen was higher the ovariectomized rats than the sham-operation, while these showed to be decreased in the estrogen, the estrogen/calcium, and the estrogen gradually reduction/calcium intensification. Fecal loss of nitrogen was decreased in tile estrogen treated group. The results in this study showed that high calcium, estrogen/calcium and estrogen gradually reduction/calcium intensification in the ovariectomized rats enhanced calcium balance compared to the ovariectomized rats without calcium treatment, but little effects on the phosphorus and nitrogen balance.

• Journal of Animal Environmental Science
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• v.17 no.sup
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• pp.35-42
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• 2011

This work was conducted to investigate the effect of dietary phytase on growth performance and excreta excretion of growing-finishing pigs for 7 days. Eighty three crossbreeds (Yorkshire ${\times}$ Landrace ${\times}$ Duroc) of growing-finishing pigs were used in this work, and divided into 6 treatments. Six treatments were compared in a $2{\times}2$ factorial arrangement with 2 groups (5 replications/group, 8 head/replications) with the additive phytase and 3 groups(growing I, II and finishing phase) with growing phases. Initial weights with growing phases were $58.6{\pm}3.9$. $83.2{\pm}3.8$ and $111.4{\pm}5.4kg$, respectively. Body weight gain was high in phytase treatment (P<0.01) and low at finishing phase. Feed conversion ratio was high in no phytase treatment and at finishing phase (P<0.05). Feed and water intakes have no significant difference with phytase existence and growing phases. Feces excretion decreased with growing phase (P<0.05), and was low at phytase treatment (P<0.05). There was no significant difference on urine excretion (P>0.05). Nitrogen (N) and phosphorus (P) intake was not found significant difference with phytase and growing phases. N excretion had no significant difference on phytase existence and growing phase (P>0.05), but P excretion decreased at phytase treatment (P<0.05). N and P excretion ratio was low at phytase treatment (P<0.05). Finally, dietary phytase resulted in improvement of growth performance and reduction of excreta excretion of growing-finishing pigs.

• Journal of Animal Science and Technology
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• v.44 no.1
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• pp.55-60
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• 2002

Two experiments were conducted to investigate the effect of dietary supplementation of plant phytase (Phytazyme$^{(R)}$) in corn-soybean meal based diets on utilization of phytase-bound phosphorus in laying hens and evaluate nitrogen(N) digestibility and phosphorus(P) availability in breeders. In the experiment one, three levels of the Phytazyme$^{(R)}$(0.05, 0.1, and 0.2% of diet) were added to diets containing a half of control TCP level(0.96%) for 4 wks. Feed consumption, egg production rate, egg weight and eggshell quality were recorded weekly. At the end of experiment, 8 birds per treatment were sacrificed, liver weight were weighed and right tibiae were removed for determination of P content. The second experiment was conducted to evaluate the P availability and nitrogen digestibility in breeders fed same diets for 2 wks. Feed and excreta were collected to determine the P and N contents for the last three days of experiment two. Addition of Phytazyme$^{(R)}$ resulted in no effects on feed intake, egg product rate, egg weight and egg shell quality. P excretion decreased and its availability enhanced as phytase supplementation increased in diets. Dietary supplementation of Phytazyme$^{(R)}$ above 0.1% level in corn-soybean meal based diets did not have an adverse effect on production and decreased level of phosphorus in excreta.