DOI QR코드

DOI QR Code

Influences of Supplemental Plant Phytase (Phytazyme®) on Performances and Phosphorus Excretion in Laying Hens

사료내 식물성 Phytase (Phytazyme®) 첨가가 산란계의 생산성 및 인 이용성에 미치는 영향

  • Kwon, S.K. (Animal Resources Research Center, Konkuk University) ;
  • Kim, S.K. (Animal Resources Research Center, Konkuk University) ;
  • An, B.K. (Animal Resources Research Center, Konkuk University) ;
  • Yang, U.M. (Animal Resources Research Center, Konkuk University) ;
  • Nam, K.T. (Dairy Extension Service Center, Hankyong National University) ;
  • Kang, C.W. (Animal Resources Research Center, Konkuk University) ;
  • Kang, S.J. (Woogene Co., Ltd)
  • 권순관 (건국대학교 동물자원연구센터) ;
  • 김성권 (건국대학교 동물자원연구센터) ;
  • 안병기 (건국대학교 동물자원연구센터) ;
  • 양운목 (건국대학교 동물자원연구센터) ;
  • 남기택 (국립한경대학교 낙농기술지원센터) ;
  • 강창원 (건국대학교 동물자원연구센터) ;
  • 강석진 (주식회사 우진)
  • Published : 2002.02.28

Abstract

Two experiments were conducted to investigate the effect of dietary supplementation of plant phytase (Phytazyme$^{(R)}$) in corn-soybean meal based diets on utilization of phytase-bound phosphorus in laying hens and evaluate nitrogen(N) digestibility and phosphorus(P) availability in breeders. In the experiment one, three levels of the Phytazyme$^{(R)}$(0.05, 0.1, and 0.2% of diet) were added to diets containing a half of control TCP level(0.96%) for 4 wks. Feed consumption, egg production rate, egg weight and eggshell quality were recorded weekly. At the end of experiment, 8 birds per treatment were sacrificed, liver weight were weighed and right tibiae were removed for determination of P content. The second experiment was conducted to evaluate the P availability and nitrogen digestibility in breeders fed same diets for 2 wks. Feed and excreta were collected to determine the P and N contents for the last three days of experiment two. Addition of Phytazyme$^{(R)}$ resulted in no effects on feed intake, egg product rate, egg weight and egg shell quality. P excretion decreased and its availability enhanced as phytase supplementation increased in diets. Dietary supplementation of Phytazyme$^{(R)}$ above 0.1% level in corn-soybean meal based diets did not have an adverse effect on production and decreased level of phosphorus in excreta.

본 연구는 식물성 phytase 처리수준이 산란계의 생산성에 미치는 영향 및 인 이용률에 미치는 영향을 검토하기 위하여 실시하였다. NRC 사양표준의 유효 인 요구량을 충족시킨 대조구와 무기태인 사용량을 50%로 제한한 처리구로 나누고, 각각의 처리구에 식물성 phytase를 각각 0.05%, 0.1% 및 0.2% 첨가하였다. 58주령의 갈색 산란계(ISA-Brown) 240수를 공시하여 총 6주간 사양실험을 실시하였고 산란종계를 이용하여 인 및 질소의 이용성을 측정하였다. 그 결과를 요약하면 다음과 같다. 1. 50% 무기태인 수준에서 식물성 phytase의 첨가에 따른 일당증체량, 산란율의 저하는 나타나지 않았다. 2. 50% 무기태인 수준에서 식물성 phytase 0.05% 첨가수준에서 일시적으로 난각질 저하 현상이 나타났으나 0.1% 이상 첨가수준에서는 난각질의 변화가 관찰되지 않았다. 3. 효소의 첨가수준이 증가함에 따라 P 배설량의 감소와 P 이용률이 개선되었다(P<0.05). 4. 식물성 phytase 첨가는 산란계의 성적 저하없이 사료내 무기태인의 사용량을 줄일 수 있음이 시사되었다.

Keywords

References

  1. AOAC. 1990. Official Methods of Analysis, 15th ed. Association of Official Analytical Chemists, Arlington, VA., USA.
  2. Bailey, C. A., Linton, S., Brister, R. and Creger, C. R. 1986. Effects of graded level of dietary phosphorus on bone mineralization in the very young poult. Poultry Sci. 65:1018-1020. https://doi.org/10.3382/ps.0651018
  3. Berka, R. M., Rey, M. W., Brown, K. M., Byun, T. and Klotz, A. V. 1998. Molecular characterization and expression of a phytase gene from the thermophilic fungus thermomyces lanugionosus. Appl. Environ Microbiol. 64:4423-4427.
  4. Duncan D. B., 1955. Multiple range and multiple F test. Biometric. 11:1-4.
  5. Keshavarz, K., 2000. Nonphytate phosphorus requirement of laying hens with and without phytase on a phase feeding program. Poultry Sci. 79:748-763.
  6. NRC. 1994. Nutrient Requirements of Poultry. 9th rev. ed. National Academic Press, Washington, D.C., U.S.A.
  7. Orban, J. I., Roland, D. A. Sr. and Bryant, M. M. 1993. Factors influencing bone mineral content density, breaking strength, and ash as response criteria for assessing bone quality in chickens. Poultry Sci. 72:437-446. https://doi.org/10.3382/ps.0720437
  8. Ravindran, V., Kornegay, E. T., Potter, L. M., Ogunabameru, B. O., Welten, M. K., Wilson, J. H. and Potchanakorn, M. 1995. An evaluation of various response criteria in assessing biological availability of phosphorus for broilers. Poultry Sci. 74:1820-1830.
  9. Roberson, K. D. and Edward, H. M. Jr. 1994. Effect of 1,25-dihydroxy cholecalciferol and phytase on zinc utilization in broiler chicks. Poultry Sci. 73:1312-1326.
  10. SAS. 1996. SAS User's Guide: Statistics. Version 6.12 ed. SAS Institute Inc., Cary, NC.
  11. Um, J. S. and Paik, I. K. 1999. Effects of microbial phytase supplementation on egg production, eggshell quality, and mineral retention of laying hens fed different levels of phosphorus. Poultry Sci. 78:75-79. https://doi.org/10.1093/ps/78.1.75
  12. Van der Klis, J. D., Versteegh, H. A. J., Simons, P. C. M. and Kies, A. K. 1997. The efficacy of phytase in corn-soybean meal based diets for laying hens. Poultry Sci. 76:1535-1542. https://doi.org/10.1093/ps/76.11.1535
  13. Waldroup, P. W. 1999. Nutritional approaches to reducing phosphorus excretion by poultry. Poultry Sci. 78:683-691. https://doi.org/10.1093/ps/78.5.683
  14. Yi, X., Kornegay, E. T. and Denbow, D. M. 1996. Effects of microbial phytase on nitrogen and amino acid digestibility and nitrogen retention of turkey poults fed corn-soybean meal diets. Poultry Sci. 75:979-990. https://doi.org/10.3382/ps.0750979
  15. 이선재, 엄재상, 남궁환, 백인기. 1999. 식물성 사료원료내 피틴태 인의 함량과 phytase activity 및 그 특성에 관한 연구. 한국영양사료학회지. 23: 501-506.