• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.94.2-95
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• 2003

E. intermedium 60-2G possessing a strong ability to solubilize insoluble phosphate, has plant growth-promoting activity, induced systemic resistance activity against scab pathogen in cucumber, and antifungal activity against various phytopathogenic fungi. The phosphate solubilizing activity of 60-2G may be mainly accomplished by production of gluconic acid through a direct extracellular oxidation of glucose by glucose dehydrogenase that required a PQQ cofactor for its activation. A pqq gene cluster conferred Phosphate-solubilizing activity in E. coli DH5${\alpha}$ was cloned and sequenced. The 6,783 bP pqq sequence had six open reading frames (from A to F) and showed 50-95％ homology to pqq genes from other bacteria. The E. coli strain expressing the pqq genes solubilized phosphate from hydroxyapatite after a pH drop to 4.0, which paralleled in time the secretion of gluconic acid. To study the role of PQQ in biocontrol traits of E. intermedium, PQQ mutants of 60-2G were constructed by marker exchangee mutagenesis. The PQQ mutants of E. intermedium were lost activities of solubilizing phosphate, growth inhibition of phytopathogenic fungi, and plant growth promotion. These findings suggest that PQQ plays an important role, possibly activation of certain enzymes, in several beneficial bacterial traits of E. intermedium by as yet an unknown mechanism.

• Molecules and Cells
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• 제40권10호
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• pp.697-705
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• 2017

The maintenance of inorganic phosphate (Pi) homeostasis is essential for plant growth and yield. Plants have evolved strategies to cope with Pi starvation at the transcriptional, post-transcriptional, and post-translational levels, which maximizes its availability. Many transcription factors, miRNAs, and transporters participate in the Pi starvation signaling pathway where their activities are modulated by sugar and phytohormone signaling. Environmental stresses significantly affect the uptake and utilization of nutrients by plants, but their effects on the Pi starvation response remain unclear. Recently, we reported that Pi starvation signaling is affected by abiotic stresses such as salt, abscisic acid, and drought. In this review, we identified transcription factors, such as MYB, WRKY, and zinc finger transcription factors with functions in Pi starvation and other environmental stress signaling. In silico analysis of the promoter regions of Pi starvation-responsive genes, including phosphate transporters, microRNAs, and phosphate starvation-induced genes, suggest that their expression may be regulated by other environmental stresses, such as hormones, drought, cold, heat, and pathogens as well as by Pi starvation. Thus, we suggest the possibility of cross-talk between Pi starvation signaling and other environmental stress signaling pathways.

• Tribology and Lubricants
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• 제17권2호
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• pp.124-129
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• 2001

The electrical and rheological behaviors of the cellulose phosphate ester suspension in the silicone oil were investigated. Cellulose phosphate ester suspension showed a typical ER response (Bingham flow behavior) upon application of an electric field. The shear stress for the cellulose phosphate ester suspension exhibited a linear dependence on the volume fraction of particles and a square power of the electric field. On the basis of the experimental results, cellulose phosphate ester suspension correlated with the conduction model of Tang et al, and found to be an ER fluid.

• Biomolecules & Therapeutics
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• 제26권1호
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• pp.29-38
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• 2018

During cancer progression, cancer cells are repeatedly exposed to metabolic stress conditions in a resource-limited environment which they must escape. Increasing evidence indicates the importance of nicotinamide adenine dinucleotide phosphate (NADPH) homeostasis in the survival of cancer cells under metabolic stress conditions, such as metabolic resource limitation and therapeutic intervention. NADPH is essential for scavenging of reactive oxygen species (ROS) mainly derived from oxidative phosphorylation required for ATP generation. Thus, metabolic reprogramming of NADPH homeostasis is an important step in cancer progression as well as in combinational therapeutic approaches. In mammalian, the pentose phosphate pathway (PPP) and one-carbon metabolism are major sources of NADPH production. In this review, we focus on the importance of glucose flux control towards PPP regulated by oncogenic pathways and the potential therein for metabolic targeting as a cancer therapy. We also summarize the role of Snail (Snai1), an important regulator of the epithelial mesenchymal transition (EMT), in controlling glucose flux towards PPP and thus potentiating cancer cell survival under oxidative and metabolic stress.

• 한국환경성돌연변이발암원학회:학술대회논문집
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• 한국환경성돌연변이발암원학회 2002년도 Current Trends in Toxicological Sciences
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• pp.48-64
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• 2002

The primary recognized health risk from common deficiencies in glucose-6-phosphate dehydrogenase (G6PD), a cytoprotective enzyme for oxidative stress, is red blood cell hemolysis. Here we show that litters from untreated pregnant mutant mice with a hereditary G6PD deficiency had increased prenatal (fetal resorptions) and postnatal death. When treated with the anticonvulsant drug phenytoin, a human teratogen that is commonly used in pregnant women and causes embryonic oxidative stress, G6PD-deficient dams had higher embryonic DNA oxidation and more fetal death and birth defects. The reported G6PD gene mutation was confirmed and used to genotype fetal resorptions, which were primarily G6PD deficient. This is the first evidence that G6PD is a developmentally critical cytoprotective enzyme for both endogenous and xenobiotic-initiated embryopathic oxidative stress and DNA damage. G6PD deficiencies accordingly may have a broader biological relevance as important determinants of infertility, in utero and postnatal death, and teratogenesis.-Nicol, C. J., Zielenski, J., Tsui, L.-C., Wells, P. G. An embryoprotective role for glucose-6-phosphate dehydrogenase in developmental oxidative stress and chemical teratogenesis.

• 한국식품과학회지
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• 제19권4호
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• pp.366-370
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• 1987

쌀 전분 호화액 (5%)의 리올로지 특성을 Brookfield 점도계로 조사하였다. 전분 호화액은 Herschel-Bulkley의 유동모형식으로 설명할 수 있었으며, 항복응력을 갖는 의가소성 유체(즉, 빙햄의가소성 유체)의 거동을 보였다. 인산염의 첨가시 쌀 전분 호화액의 항복응력 값은 현저히 감소하였다. 멥쌀 전분 호화액의 경우 인산염에 의하여 의가소성이 강해지는 현상을 보였으나, 찹쌀 전분 호화액의 경우에는 반대의 경향을 보였다. 전분 호화액을 $90^{\circ}C$에서 1시간 유지한 경우 멥쌀 전분 호화액의 항복 응력 값은 다소 감소하였으나, 찹쌀 전분 호화액은 10배 정도 감소하였다. 그러나 인산염의 첨가시에는 시료 전분 모두 항복응력 값이 감소하였으며, 그 정도는 아끼바레 전분 호화액이 가장 현저하였다. 또 인산염은 쌀 전분 호화액의 유동 거동지수 값에는 영향을 주지 않았으나, 점조도 지수값을 감소시켰다.

• 자연과학논문집
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• 제9권1호
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• pp.53-56
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• 1997

Cyanobacteria Chlorogloea fritschii를 여러 생장조건(인산 충분 혹은 결핍)에서 배양한 후 poly-$\beta$-hydroxybutyrate(PHB)를 추출하여 그의 구조를 적외선 분광법에 의해 분석하였다. 이들은 공통적으로 C=O의 신축 진동에 의한 1700-1800 $cm^-1$영역에서 대단히 강한 흡수 피이크를 나타내고 또한 2900$cm^-1$에서 C-H의 비대칭 및 대칭 신축 진동흡수 피이크를 나타냄으로써 PHB의 특징을 잘 나타내고 있었다. 그러나 생장조건에 따라 C-H의 신축 진동흡수 피이크 세기에 변화를 관측할 수 있었으며 인산 결핍 생장조건에서 C-H의 신축 진동흡수 피이크의 세기는 나머지 피이크에 비해 증가된 양상을 나타냄으로 인산의 공급여하에 따른 PHB구조의 변화를 시사하였다.

• 한국토양비료학회지
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• 제45권3호
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• pp.428-434
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• 2012

A fungal strain, capable of solubilizing insoluble phosphate under diverse temperature, pH and salt conditions was isolated from Waste Mushroom bed of Agaricus bisporus in South Korea. Based on 18S rRNA analysis, the strain was identified as Aspergillus awamori bxq33110. The strain showed maximum phosphate solubilization in AYG medium (525 ${\mu}g\;mL^{-1}$) followed by NBRIP medium (515 ${\mu}g\;mL^{-1}$). The strain solubilized $Ca_3(PO_4)_2$ to a greater extent and rock phosphate and $FePO_4$ to a certain extent. However $AlPO_4$ solubilizing ability of the strain was found to be very low. Glucose at the rate of 2% ($561{\mu}g\;mL^{-1}$) was found be the best carbon source for Aspergillus awamori bxq33110 to solubilize maximum amount of phosphate. However, no significant difference ($P{\leq}0.05$) in phosphorus solubilization was found between 1% and 2% glucose concentrations. $(NH_4)_2SO_4$ was the best nitrogen source for Aspergillus awamori bxq33110 followed by $NH_4Cl$ and $NH_4NO_3$. At pH 7, temperature $30^{\circ}C$ and 5% salt concentration (674 ${\mu}g\;mL^{-1}$) were found to be the optimal conditions for insoluble phosphate solubilization. However, strain Aspergillus awamori bxq33110 was shown to have the ability to solublize phosphate under different stress conditions at $30-40^{\circ}C$ temperature, pH 7-10 and 0-10% salt concentrations indicating it's potential to be used as bio-inoculants in different environmental conditions.

• 한국초지조사료학회지
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• 제42권2호
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• pp.114-119
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• 2022

The objective of this study was to determine phosphorus effects on drought stress-induced oxidative stress in Kentucky bluegrass. Drought stress was induced by reducing of water to plants in pots. Two types of phosphorus were applied as potassium phosphate (P) or potassium phosphonate (PA). Application of phosphorus was efficient to ameliorate the adverse effects of drought. Osmotic potential, total chlorophyll and carotenoid content were significantly decreased by drought stress, but was relieved by P or PA application. Superoxide (O₂^•-) concentration was significantly increased more than 14-fold under drought-stressed plants, was accompanied with increase of hydrogen peroxide (H₂O₂) and lipid peroxidation (MDA). However, malondialdehyde (MDA) was much less in P or PA applied plants under drought stress condition. Activities of catalase (CAT), ascorbate peroxidase (APX) and guaiacol-peroxidase (GPX) were largely increased by drought stress and its increase rate was much higher in P or PA applied plants except APX. These results indicate that drought stress-induced oxidative stress is alleviated by P or PA application due to the increase of activities of antioxidant enzymes.

• KSTLE International Journal
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• 제3권1호
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• pp.23-25
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• 2002

The electrorheological (ER) behavior of amino coated chitosan phosphate suspension in silicone oil is investigated. Amino coated chitosan phosphate suspension shows a typical ER response (Bingham flow behavior) upon application of an electric field due to the polarizability of phosphoryl polar group. The shear stress for the suspension exhibits a linear dependence on an electric field power of 1.94. The values of structure factors $A_s$obtains 2 far mine coated chitosan phosphate suspension and it may be due to the formation of multiple chains upon application of the electric field. Throughtout the experimental results, amino coated chitosan phosphate suspension shows good dispersion stability and found to be an anhydrous ER fluid.