• Journal of The Korean Society of Grassland and Forage Science
• /
• v.28 no.3
• /
• pp.165-170
• /
• 2008

Timothy (Phleum pratense L.) is an important grass species as forage. In order to optimize tissue culture conditions of timothy, the effects of plant growth regulators on callus induction and plant regeneration was investigated with mature seeds of colt cultivar. The optimal concentration of 2,4-D for the induction of primary callus from mature seeds was 3 mg/L. The highest embryogenic callus frequenc (25%) was observed when the mature seed were cultured on MS medium supplemented with 3 mg/L 2,4-D and 0.1 mg/L BA. The highest plant regeneration frequency was observed when type B callus was transferred to N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Regenerated plants were grown normally when shoots were transplanted to the soil. A short tissue culture period and regeneration system would be beneficial for molecular breeding of timothy by the production of transgenic plant.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.23 no.3
• /
• pp.211-218
• /
• 2003

This study was conducted to select the promising varieties of timothy (va.) Climax, Itasca, Alma, Comtal, Liphlea, Erecta, Argus and Kunpu at Daekwanryong(altitude 800m a.s.l.) and Namwon(altitude 450m a.s.l.) branch, and orchard grass (va.) Potomac, Warrior, Ambassador, Hapsung 2, 20, 93E and Kitamidori at Daekwanryong branch of National Livestock Research Institute, from 1999 to 2002. Among the timothy varieties, leafness of Liphlea, Erecta and Kunpu was greatest. Heading time of Liphlea and Kunpu was earliest and plant length of Kunpu was longest. Also, winter survival of Erecta and Kunpu was greatest with 94.0%. Among the orchardgrass varieties, although heading time of Hapsung 20 was late, it's leafness was the best and plant length was the longest in all varieties. Also, winter survival of Hapsung 20 was the highest in all varieties. In Daekwanryong and Namwon, dry matter yield of timothy(va.), Kunpu was highest(P<0.05) among the timothy varieties with 9,493kg and 13,890kg/ha, respectively. Dry matter yields of orchardgrass(va.), Hapsung 2 and Hapsung 20 were greatest among the varieties with 8,690kg and 8,818kg/ha, respectively. In two areas, average ADF concentration of Climax was lowest with 33.4%, and NDF concentration of Argus was lowest with 65.4% in all varieties. Average crude protein content of Alma was the highest with 16.5% and that of Kunpu was the lowest with 13.9%. Average crude protein yield of Kunpu was the highest with 1,710.5kg/ha in all timothy varieties. The ADF concentration of Hapsung 20 was lowest (33.4%), and NDF concentration of 93E was lowest (67.5%). Crude protein content (13.5%) and crude protein yield (1,173.2kg/ha) of Hapsung 2 were highest. These results indicate that Kunpu and Liphlea would be the promising varieties of timothy in Daekwanryong and Namwon, and also, Hapsung 2 of orchardgrass would be the promising varieties in Daekwanryong.