• Journal of the Optical Society of Korea
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• v.19 no.1
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• pp.22-28
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• 2015

We propose a new method for improving the phase contrast of a multiphase digital holographic microscope using a spatial light modulator (SLM). Using the SLM as the annulus, our method improves the light contrast of the object edge to achieve higher accuracy. We demonstrate a digital holographic microscopy technique that provides a 30% improvement in the phase contrast compared to conventional microscopy, which utilizes a mechanical annulus. The phase-contrast improvement allows the 3D reconstructed hologram to be determined more precisely.

• Polymer(Korea)
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• v.27 no.5
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• pp.413-420
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• 2003

Fluorescence microscope technique was employed for the characterization of phase separation behavior of a 4-chloro-7-nitrobenzofurazan labeled polystyrene (PS) / polybutadiene (PB) blend in dioctyl phthalate under steady shear. It was confirmed that the fluorescence microscope images reflect the real phase morphology by comparing with the images of phase contrast microscope. Comparing the fluorescence intensities from the phase separated domain (PS rich) and continuous phase (PB rich), the composition difference between these two phases were deduced. The observed shear dependence of compositional change is then used to confirm that the phase diagram is indeed shifted under the steady shear.

• Progress in Medical Physics
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• v.22 no.3
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• pp.117-123
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• 2011

Synchrotron radiation (SR) imaging enables us to observe internal structures of biologic samples without staining. In this study, we obtained X-ray microscopic images of human breast tissues with 11.1 KeV hard X-ray microscope of the Pohang light source and used zone plates and phase-contrast technique to get high resolution X-ray images. Hard X-ray microscopic images of fibrocystic change and breast cancer tissues with a spatial resolution of 60 nm were obtained and from these images, we could observe the micro-structures of human breast tissue. Also we analyzed and compared these images, which revealed distinct features of each condition. In conclusion, SR imaging with phase-contrast hard X-ray microscope for medical application, especially in breast disease can give some useful information for clinical research.

• The Journal of the Acoustical Society of Korea
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• v.18 no.3
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• pp.55-61
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• 1999

In this study, we have constructed the acoustic microscope using quadrature technique and analyzed the relative variation of image intensity and the quality of image by reconstructing the amplitude and phase image for surface defects with tiny hight variation. In this experiment, we have constructed the scanning acoustic microscope using the focused transducer with 3㎒ center frequency and the quadrature detector. And we have fabricated aluminum samples with round defects whose depth is different and reconstructed the amplitude and phase images for the samples. One sample has round defects with 2㎜ diameter and 100㎛ depth and the other has round defects with 4㎜ diameter and 5㎜ depth. In the result of line scanning for the sample with 100㎛ round defects, it has been shown that the variation rate of amplitude image intensity is 7% and the variation rate of phase image intensity is 89%. The phase image has better contrast than amplitude image for the sample. In contrast to this, the amplitude image has better contrast than phase image for the sample with 5㎜ depth's defects. Accordingly there is big difference between amplitude image and phase image for depth variation of defects whose boundary is 1 wavelength. Consequently the acoustic microscope using quadrature detector can be evaluated efficiently more than using envelope detector, for detecting defects which have height variation less than 1 wavelength. And also the phase image and the amplitude image can be used for detecting defects of tiny height variation with complimentary relation.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2000.05b
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• pp.323-326
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• 2000

In this study, we used Brewster-Angle Microscope(BAM) to study on the molecular orientation of monolayer on the water surface. The behaviors of molecules on three different subphase have been observed. Reproducible $\pi$-A isotherm have been obtained only on information about phase transition by molecular area. BAM facilitates the observation of morphology by optical anisotropy and thickness in monolayer and multilayers as BAM is shown to be sensitive to anisotropy of film. Every transition was found by BAM technique, either as a dramatic change in degree of contrast or as a sudden alteration of molecular action and $\pi$-A isotherm.

• Macromolecular Research
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• v.14 no.1
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• pp.45-51
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• 2006

We conducted simultaneous, small-angle, X-ray scattering/differential scanning calorimetry (SAXS/DSC) and simultaneous, wide-angle, X-ray scattering (WAXS)/DSC measurements for a polymer blend of poly($\varepsilon$-caprolactone)/poly(ethylene glycol)(PCL/PEG). The time-dependent SAXS/DSC and WAXS/DSC results, measured while the system was quenched below the melting temperature of PCL from a melting state, revealed the competitive behavior between liquid-liquid phase separation and crystallization in the polymer blend. The time-dependent structural evolution extracted from the SAXS/WAXS/DSC results can be characterized by the following four stages in the PCL crystallization process: the induction (I), nucleation (II), growth (III), and late (IV) stages. The influence of the liquid-liquid phase separation on the crystallization of PCL was also observed by phase-contrast microscope and polarized microscope with 1/4$\lambda$ compensator.

• Proceedings of the KSME Conference
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• 2008.11a
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• pp.322-326
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• 2008

In this paper, we have studied the images which have been reconstructed by using combination of images acquired by the variation of operating frequency. When inner images have been reconstructed, they have been superposed by the surface state effect. In this case, the images of the phase object can be enhanced by the contrast of inner images. There is a kind of specimen, one is a reference block having 1/4T, 1/2T, 3/4T side drilled holes as main run piping material of the steam generator in nuclear power plants. It has been shown that the two results of defect shapes have better than before in this processing and phase contrast grow about twice. And we have constructed the acoustic microscope by using a quadrature detector that enables to acquire the amplitude and phase of the reflected signal simultaneously. Further more we have studied the reconstruction method of the amplitude and phase images, the enhancement method of the defect images' contrast.

• Proceedings of the KSME Conference
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• 2001.06b
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• pp.176-180
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• 2001

In this paper, we have studies the images have been reconstructed by using combination of images which has been acquired by the variation of operating frequency. When inner images has been reconstructed, inner image has been superposition by surface state effect. In this case, image enhancement the phase object and enhance the contrast of inner image. In the result of the specimen for the round defect with 2mm diameter, for the types of the depth are 1.5mm, 2mm, 2.5mm, and 3mm, it has been show that the shape of defect has better than before this processing and phase contrast grow large twice. And we have constructed the acoustic microscope by using quadrature detector that is able simultaneously to acquired the amplitude and phase of the reflected signal. Father more we have studied the reconstruction method of the amplitude and phase images and the enhancement method of the defect images' contrast.

• KSTLE International Journal
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• v.1 no.2
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• pp.69-75
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• 2000

The paper compares topography, phase contrast and force spectroscopy in atomic force microscopy data for evaluating the microheterogeneity of surface layer. The worn surface of ion-plated TiN coating was measured using both a laboratory-built and a commercial AFM. The results of analysis revealed structural and micromechanical heterogeneity of the worn surfaces. We demonstrated that the phase image allows relatively qualitative estimation of elastic modulus of the sample surface. The tribolayer formed in the worn surface possessed much lower stiffness than the original coating. It is shown that the most stable phase imaging is provided with a stiff cantilever. In this case, phase contrast is well conditioned, first of all, by microheterogeneity of elastic properties of the investigated surfaces. In this study an attempt was also made to correlate the results of phase imaging with that of the farce spectroscopy. The joint analysis of information on the surface properties obtained by the phase imaging and quantitative data measured with the force spectroscopy methods allows a better understanding of the nature of the surface micromechanical heterogeneity.

• Journal of the Korean Ceramic Society
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• v.30 no.11
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• pp.919-924
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• 1993

Domain structure and the spatial arrangement of tetragonal and rhombohedral phases in PZT ceramics at MPB composition were investigated with a transmission electron microscope. Fringe Contrast and doublet spot splitting were observed in the image mode and the selected area diffraction pattern, respectively. Besides, triplet spot splitting was also observed in the other part of the specimen. These observations indicate that both the single phase regions and the regions which are comosed of alternatively arranged tetragonal and rhombohedral domains coexist in a PZT ceramics at the MPB composition.