• The Plant Pathology Journal
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• 제15권5호
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• pp.302-307
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• 1999

Occurrence of soft rots was observed on Dendrobium phalaenopsis and Phalaenopsis sp. that were grown at the greenhouses in Sunchon and Kwangyang areas, Chonnam province of Korea in 1997 and 1998. Typical soft rot symptom appeared frequently on young plants of D. phalaenopsis and Phalaenopsis sp. Soft rot symptom usually appeared on old leaves of D. phalaenopsis, and extended into whole leaves, accompanying blighting of whole plants. Symptom began as a small water-soaked lesion on old leaves of Phalaenopsis sp., which enlarged rapidly on the leaves and eventually resulted in soft rots of whole plants. The causal organism isolated from the infected lesions was identified as Erwinia chrysanthemi based on its pathogenicity, physiological and biochemical characteristics, and the results of the BIOLOGTM program. The bacterial soft rot caused by e. chrysanthemi was firstly describe din D. phalaenopsis and Phalanopsis sp. in Korea.

• Journal of Plant Biotechnology
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• 제39권4호
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• pp.225-234
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• 2012

This report describes recent advances in tissue culture and genetic transformation of commercial Phalaenopsis. Recently, an importance of Phalaenopsis has been increased due to its popularity with beautiful flowers and is widely used for pot plants as well as cut-flower. Its use is rapidly enlarging in worldwide. Thus, demands for the release of new elite cultivars in Phalaenopsis have been increased. During the last several decades, some critical progresses have been made in tissue culture and genetic transformation in Phalaenopsis species. Cooperation with these biotechnological methods are supposed to promote the release of commercial Phalaenopsis cultivars in the near future. Until now, no technical review on tissue culture and genetic transformation in Phalaenopsis has been reported in Korea. Therefore, we inquired the brief history and techniques of tissue culture system in Korea.

• Current Research on Agriculture and Life Sciences
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• 제32권4호
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• pp.179-183
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• 2014

Optimization of the protocorm-like body (PLB) formation of Phalaenopsis and Dendrobium hybrids was performed by determining the effects of plant growth regulators (PGRs) and different parts and division sizes of the PLB. For both genera, the base part was the best for the proliferation of PLBs, yielding the highest number of PLBs on a PGR-free medium for Phalaenopsis and medium containing $0.1{\mu}M$ NAA and $10.0{\mu}M$ BAP for Dendrobium. As regards the division size, four-division sections resulted in a higher PLB formation efficiency for Phalaenopsis, while two-division sections produced a higher PLB formation efficiency for Dendrobium. It is expected that these findings will be applicable to efficient PLB formation of other Phalaenopsis and Dendrobium orchids.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.173-173
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• 2017

Phalaenopsis orchid is one of the most important flowers in flower industry. We conducted an experiment to find out the response of different concentration of chlorocholine chloride (CCC) for the in vitro regeneration of protocorm like bodies (PLBs) of Phalaenopsis 'Fmk02010'. We used five different concentrations of CCC and these were 0 (control), 0.01, 0.1, 1 and $10mgL^{-1}$ in modified MS medium and cultured for 42 days. We added two major salts ammonium nitrate ($412.5mgL^{-1}$) and potassium nitrate ($950.0mgL^{-1}$) to the MS medium for the modification. Maximum numbers of PLBs were found from media with $0.01mgL^{-1}$ of CCC (15.667) and maximum fresh weight (0.211 g) as well. The 100% PLB formation rate was also found from $0.01mgL^{-1}$ of CCC. We found 58.83% variation in number of PLBs ($R^2=0.5883$) and 47.44% variation in fresh weight ($R^2=0.4744$) to the different CCC concentrations. Our study suggested that increase in the CCC concentration negatively affect the PLBs organogenesis of Phalaenopsis. We can suggest that the addition of very low concentration of CCC in plant culture medium can increase the number, formation rate and fresh weight of PLBs of Phalaenopsis.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.177-177
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• 2017

Phalaenopsis orchid is one of the most important flowers in flower industry. We conducted an experiment to find out the response of different concentration of chlorocholine chloride (CCC) for the in vitro regeneration of protocorm like bodies (PLBs) of Phalaenopsis 'Fmk02010'. We used five different concentrations of CCC and these were 0 (control), 0.01, 0.1, 1 and $10mgL^{-1}$ in modified MS medium and cultured for 42 days. We added two major salts ammonium nitrate ($412.5mgL^{-1}$) and potassium nitrate ($950.0mgL^{-1}$) to the MS medium for the modification. Maximum numbers of PLBs were found from media with $0.01mgL^{-1}$ of CCC (15.667) and maximum fresh weight (0.211 g) as well. The 100% PLB formation rate was also found from $0.01mgL^{-1}$ of CCC. We found 58.83% variation in number of PLBs ($R^2=0.5883$) and 47.44% variation in fresh weight ($R^2=0.4744$) to the different CCC concentrations. Our study suggested that increase in the CCC concentration negatively affect the PLBs organogenesis of Phalaenopsis. We can suggest that the addition of very low concentration of CCC in plant culture medium can increase the number, formation rate and fresh weight of PLBs of Phalaenopsis.

• 식물병연구
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• 제9권3호
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• pp.149-152
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• 2003

전남 광양읍 봉강면의 양란 재배농가에서 재배되고 있는 덴파레에 검은점무늬병이 발생하였다. 자연 발병한 덴파레의 잎에는 초기에 검은색의 작은 점무늬가 형성되어 점차 진전되면서 잎 전체가 누렇게 변하면서 고사해 버리며 소엽병의 마디가 약화되어 낙엽이 지고 심하면 식물체 전체가 말라죽었다. 검은점무늬병에 감염된 덴파레의 병환부로부터 분리된 병원균은 감자한천배지 상에서 초기에 흰색을 띠며, 점차 시간이 지남에 따라 진한 보라색을 띠었으며, 균사생장 적온은 $25^{\circ}C$였다. 덴파레 잎의 검은 점무늬 병반으로부터 분리한 병원균은 균학적 특성 및 병원성 검정 결과 Fusarium moniliforme로 동정되었다. 병원균은 상처 접종에 의해서만 덴파레에 병원성을 나타내었고, 호접란과 심비디움에 대해서도 모두 병원성을 나타내었다. 따라서 이 병을 Fusarium moniliforme에 의한 덴파레 검은점무늬병으로 명명할 것을 제안한다.

• 화훼연구
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• 제17권3호
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• pp.165-171
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• 2009

팔레높시스 화경의 모든 부위를 사용한 화경절편조직을 기내 배양하여 효율적이고 변이 발생률이 낮은 균일한 식물체를 일시에 대량생산하기 위하여 Hyponex와 생장조절물질 TDZ, BA의 적정 농도를 규명하고자 하였다. 두 공시 품종은 Hyponex, TDZ, BA 농도별로 floral stem, shoot, 다신초의 기관형성 발생에서 차이를 보였다. 팔레높시스의 화경절편조직 배양에 적절한 Hyponex의 농도는 $4g{\cdot}L^{-1}$ 가 가장 효과적이었으며, 다신초 형성에 효과적인 TDZ의 농도는 $0.3mg{\cdot}L^{-1}$이고, BA의 농도는 $5mg{\cdot}L^{-1}$ 이었다. 화경절편조직 배양 시 Hyponex $4g{\cdot}L^{-1}$을 기본배지로 하여 TDZ와 BA의 효과를 비교한 결과 TDZ가 더 효과적이었다.

• BMB Reports
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• 제40권6호
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• pp.845-852
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• 2007

The highly evolved flowers of orchids have colorful sepals and fused columns that offer an opportunity to discover new genes involved in floral development in monocotyledon species. In this investigation, we cloned and characterized the homologous PISTALLATA-like (PI-like) gene PhPI15 ($\underline{Ph}alaenopsis$ $\underline{PI}$ STILLATA # $\underline{15}$), from the Phalaenopsis hybrid cultivar. The protein sequence encoded by PhPI15 contains a typical PI-motif. Its sequence also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Southern analysis showed that PhPI15 was present in the Phalaenopsis orchid genome as a single copy. Furthermore, it was expressed in all the whorls of the Phalaenopsis flower, while no expression was detected in vegetative organs. The flowers of transgenic tobacco plants ectopically expressing PhPI15 showed male-sterile phenotypes. Thus, as a Class-B MADS-box gene, PhPI15 specifies floral organ identity in orchids.

• 원예과학기술지
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• 제35권1호
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• pp.21-29
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• 2017

This study was conducted to determine the effects of artificial lighting sources on vegetative growth of Phalaenopsis and Doritaenopsis (an intergeneric hybrid of Doritis and Phalaenopsis) orchids. One - month - old plants were cultivated under fluorescent lamps, cool - white light - emitting diodes (LEDs), or warm - white LEDs at 80 and $160{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. The blue (400 - 500 nm) : green (500 - 600 nm) : red (600 - 700 nm) : far - red (700 - 800 nm) ratios of the fluorescent lamps, cool-white LEDs, and warm-white LEDs were 1 : 1.3 : 0.8 : 0.1, 1 : 1.3 : 0.6 : 0.1, and 1 : 2.7 : 2.3 : 0.4, respectively. Each light treatment was maintained for 16 weeks in a closed plant-production system maintained at $28^{\circ}C$ with a 12 h photoperiod. The longest leaf span, as well as the leaf length and width of the uppermost mature leaf, were observed in plants treated with warm-white LEDs. Plants grown under fluorescent lamps had longer and wider leaves with a greater leaf span than plants grown under cool-white LEDs, while the maximum quantum efficiency of photosystem II was higher under cool-white LEDs. The vegetative responses affected by different lighting sources were similar at both 80 and $160{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Leaf span and root biomass were increased by the higher light intensity in both cultivars, while the relative chlorophyll content was decreased. These results indicate that relatively high intensity light can promote vegetative growth of young Phalaenopsis plants, and that warm - white LEDs, which contain a high red-light ratio, are a better lighting source for the growth of these plants than the cool-white LEDs or fluorescent lamps. These results could therefore be useful in the selection of artificial lighting to maximize vegetative growth of Phalaenopsis plants in a closed plant - production system.

• 화훼연구
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• 제17권1호
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• pp.29-35
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• 2009

분화 호접란의 이산화탄소 시비 농도와 공급시간을 구명하고자, Phalaenopsis 'Brother Spring Dance', 'Brother Girl' 및 'Queen Bear' 품종을 2개월, 7개월 그리고 12개월 생장된 묘에 이산화탄소 농도 1,000 및 $2,000{\mu}mol{\cdot}mol^{-1}$을 06:00~12:00시, 00:00~06:00시와 18:00~24:00시에 99일간 처리하였다. 호접란 모든 처리 품종은 이산화탄소 농도 $1,000{\mu}mol{\cdot}mol^{-1}$ 보다 $2,000{\mu}mol{\cdot}mol^{-1}$ 에서 엽수, 엽장, 엽폭, 뿌리길이 및 생체중 등의 생육이 촉진되었고, $CO_2$ 공급시간은 00:00~06:00시가 가장 효과적이었다.