• Journal of Nutrition and Health
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• v.50 no.1
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• pp.25-31
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• 2017

Purpose: Neuroinflammation is mediated by activation of microglia implicated in the pathogenesis of neurodegenerative disorders such as Alzheimer's disease and Parkinson's disease. Inhibition of neuroinflammation may be an effective solution to treat these brain disorders. Petalonia binghamiae is known as a traditional food, based on multiple biological activities such as anti-oxidant and anti-obesity. In present study, the anti-neuroinflammatory potential of Petalonia binghamiae was investigated in LPS-stimulated BV2 microglial cells. Methods: Cell viability was measured by MTT assay. Production of nitric oxide (NO) was examined using Griess reagent. Expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) was detected by Western blot analysis. Activation of nuclear factor ${\kappa}B$ ($NF-{\kappa}B$) signaling was examined by nuclear translocation of $NF-{\kappa}B$ p65 subunit and phosphorylation of $I{\kappa}B$. Results: Extract of Petalonia binghamiae significantly inhibited LPS-stimulated NO production and iNOS/COX-2 protein expression in a dose-dependent manner without cytotoxicity. Pretreatment with Petalonia binghamiae suppressed LPS-induced $NF-{\kappa}B$ p65 nuclear translocation and phosphorylation of $I{\kappa}B$. Co-treatment with Petalonia binghamiae and pyrrolidine duthiocarbamate (PDTC), an $NF-{\kappa}B$ inhibitor, reduced LPS-stimulated NO release compared to that in PB-treated or PDTC-treated cells. Conclusion: The present results indicate that extract of Petalonia binghamiae exerts anti-neuroinflammation activities, partly through inhibition of $NF-{\kappa}B$ signaling. These findings suggest that Petalonia binghamiae might have therapeutic potential in relation to neuroinflammation and neurodegenerative diseases.

• ALGAE
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• v.17 no.3
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• pp.135-144
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• 2002

Scytosiphonaceae is an acetocarpalean brown algal family, that is a recent focus of synstematics and marine biodiversity. We describe Petalonia zosterifolia and Scytosiphon gracilis from Korea for the first time. P. zosterifolia occurred on the East coast, and had flat, linear and solid thalli. S. gracilis was found in Jeju, and had cylindircal to flat and hol-low thalli. However, these two species are so similar that it is difficult to identify by morphology alone. In order to determine if the nuclear DNA reveals the distinctness of both species and to know their phylogenies, the ITS region sequences were newly detrmined in 22 samples of P. zosterifolia, Scytosiphon gracilis, and other three members of the genera from Korea. We found 0.12% variation among samples of P. zosterifolia from different locations, and no variation between S. gracilis samples from diferent years, but extensive interspecific divergences (13.62-22.83%) of each species to other members in Petalonia and Scytosiphon . The ITS sequence dta consistently showed a close relationship between P. zosterifolia and S. gracilis. This result is congruent with morphology and with the published data of plastid rbc and partial nrDNA large subunit gene sequences, and suggests that P. zosterifolia and S. gracilis might have diverged from the most recent common ancestor.

• ALGAE
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• v.18 no.4
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• pp.333-339
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• 2003

To establish a cultivation method of Petalonia fascia, seeds and seedlings cultures and growth tests were performed at the Daeri aquafarm in Haeui, Shinan, Jeollanamdo, Korea. Gametes were easily released from the mature plurilocular sporangia. They developed to crustose discoidal stolons and grew to filamentous and discoidal stolons. The indoor seeding was performed by using the 100-150 ${\mu}m$long fragments of stolons on Porphyra nets and the erect thalli developed from the cuttings when the seawater temperatures were 10-15$^{\circ}C$. In the experimental cultivation in the sea, 1-2mm long plantlets were found after 15 days of cultivation; after two months thalli grew to their maximal size of 215-355 mm long blades; after three months the length of thalli began to decrease due to distal disintegration and the plant color changed to yellow and epiphytic diatoms were attached on the thalli, which deteriorated the quality of products. The cultivation of P. fascia by the regeneration of filamentous-discoidal complexes was carried out successfully for the first time in Korea.

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2002.05a
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• pp.23-23
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• 2002

• Journal of Plant Biology
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• v.35 no.2
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• pp.125-134
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• 1992

Taxonomic account is given to the five species of Scytosiphonaceae, Colpomenia peregrina (Sauvageau) Hamel, Endarachne binghamiae J. Agardh, Hydroclathrus clathratus (C. Agardh) Howe, Petalonia fascia (O. F. Muller) Kuntze, and Scytosiphon lomentaria (Lyngbye) Link, collected from Ullungdo Island, Korea. C. peregrina, introduced for the first time in Korea, is similar to C. sinuosa (Roth) Derbes et SolieI in outer appearance but is distinguished by absence of cuticle over the plurilocular sporangial sori. E. binghamiae accords well with American plants but varies in height and width of the thallus according to seasons. H. clathratus has small cortical cells and large clear medullary cells. P fascia is characterized by one to two celled small cortical layer and two to four celled large medullary layer. S. lomentaria shows variation in gross morphology, especially in height and width of thallus according to seasons.easons.

• ALGAE
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• v.21 no.2
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• pp.175-183
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• 2006

Recent phylogenetic studies of scytosiphonacean brown algae show many conflicts with current classification. In order to clearly define the phylogenetic relationships of the family, we newly sequenced the photosystem I coding psaA gene (1488 base pairs) from 13 taxa (15 samples), of the family, and, for comparison, rbcL from four taxa. The psaA region has more informative sites (17.9%) than the rbcL (13.1%) and the number of nodes supported by over 50% bootstrap values is more in the psaA phylogeny (53 /57 nodes; 93%) than in the rbcL (47/63 nodes; 74.6%). The psaA phylogenies are basically congruent with the rbcL trees, recognizing two major groups in the monophyletic Scytosiphonaceae. The first group included Myelophycus, Petalonia, Scytosiphon, and elongate sack-shaped species of Colpomenia, primarily cold temperate elements with unilocular zoidangia on sporophytes. The second group, although not resolved, consisted of Hydroclathrus, Chnoospora, Rosenvingea, and ball-shaped Colpomenia, primarily warm-temperate taxa with both unilocular and plurilocular zoidangia on sporophytes. Chnoospora is not monophyletic, as was previously shown the paraphyly of Colpomenia, Petalonia, and Scytosiphon. Hydroclathrus clathratus from Korea and Japan was not monophyletic. Our studies show that gametophytic characters are the main source of conflict for the present taxonomy of the family. The psaA region is a useful tool for resolution of phylogenetic relationships within the Scytosiphonaceae.

• ALGAE
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• v.34 no.3
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• pp.229-236
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• 2019

Despite the abundance of seaweeds from Ulleungdo Island, genetic diversity and distribution of edible brown algae from the island remain unstudied. We analyzed mitochondrial cox3 sequences from 86 specimens collected in the island and from the nearby Korean Peninsula. Our cox3 phylogeny for the first time confirmed the occurrence of fives species from Ulleungdo Island; Petalonia binghamiae, P. fascia, Planosiphon zosterifolius, and two cryptic species previously identified as Scytosiphon lomentaria. P. binghamiae was relatively homogeneous with three haplotypes. P. fascia comprised four haplotypes, which were grouped into two genetic lineages. S. lomentaria was heterogeneous with nine haplotypes and was divided into two cryptic species; one species clustered with taxa from cold waters while the other clustered with taxa from temperate and cold waters. Low genetic diversity in P. binghamiae while high genetic diversity in S. lomentaria from Ulleungdo Island are comparable to patterns observed from other species from the Korean peninsula. Ulleungdo Island, although small in size, is an ideal field laboratory to investigate genetic diversity and distributions of economic marine algae.

• Journal of Life Science
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• v.26 no.5
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• pp.555-562
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• 2016

In this study, we investigated the effects of Undaria pinnatifida (UP), Petalonia binghamiae (PB) and Punctaria latifolia (PL) extracts on the inhibition of proliferation and apoptosis in human gastric and breast cancer cells. AGS, MDA-MB-231 and SK-BR-3 cells were treated with 0, 50, 100, and 200 μg/ml concentrations of the extracts to determine their anti-proliferative effects, using the MTT assay. The UP, PB and PL extracts inhibited proliferation of AGS, MDA-MB-231 and SK-BR-3 cells in a dose-dependent manner, and the PL extract was found to be the most effective. DAPI staining was also performed to determine changes in the cell nucleus. Further, the AGS, MDA-MB-231 and SK-BR-3 cells were treated with 0, 50, 100, and 200 μg/ml of only the PL extract. DAPI staining showed increased chromatin condensation, which is indicative of apoptosis, in the 200 μg/ml group. The expression of the Bax, Bcl-2, and PARP proteins in AGS, MDA-MB-231 and SK-BR-3 cells treated with the PL extract was also determined by western blot analysis. The expression of Bax (a pro-apoptotic protein) and cleaved-PARP was increased, whereas the expression of Bcl-2 (an anti-apoptotic protein) was decreased compared with the control. These findings indicate that the PL extract may have potential as an alternative anticancer drug and nutraceutical.

• Journal of Plant Biology
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• v.34 no.1
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• pp.45-51
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• 1991

Morphological variability was quantified for Endarachne binghamiae J. Agardh from five local populations collected in December, 1989 and in March, 1990. The sampling sites were chosen at intervals of 50'N along the east coast of Korea. Fifty individuals were haphazardly collected for the relationship between the blade length and reproduction, among which twenty five individuals were randomly selected for morphological variation. The Korean plants of E. binghamiae had the capacity of reproduction at an early stage of growth. The holdfast diameter and the plurangial length showed clinal variation of increment from the north to the south whereas the cryptostomata from the south to the north. The holdfast diameter, the blade thickness, the number of plurangial blades and the cryptostomata increased from December to March, when the blade length decreased. Endarachne is distinguished by the densely interwoven hypha I cells in the medulla, [our to eighteen rows of the plurangial compartments, and absence of paraphyses between plurangia. Therefore E. binghamiae should not be combined into Petalonia, but rather the Soviet plants of P. zosterijolia may be renamed as a taxon belonging to the genus Endarachne.rachne.

• Natural Product Sciences
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• v.18 no.2
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• pp.130-136
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• 2012

In the present work, we have collected 19 species of macroalgae (9 Phaeophta and 10 Rhodophyta) f rom all around of Korea: Dictyopteris divaricata, D. prolifera, Myelophycus cavus, Papenfussiella kuromo, Petalonia zosterifolia, Petrospongium rugosum, Rugulopteryx okamurae, Sargassum fulvellum, S. muticum, Callophyllis japonica, Gloiopeltis tenax, Gracilaria longissima, Gracilaria vermiculophylla, Grateloupia asiatica, Grateloupia lanceolata, Grateloupia sparsa, Grateloupia turuturu, Grateloupia sp, and Polyopes affinis. The macroalgal species were extracted by 70% ethanol (EtOH) for 24 h and evaluated its inhibitory effects on ${\alpha}$-glucosidase. Among ethanol extracts, Myelophycus cavus showed the most effectively inhibitory activity ($IC_{50}$, 2.17 ${\mu}g/ml$) against ${\alpha}$-glucosidase, followed by Sargassum fulvellum (<$IC_{50}$, 8.13 ${\mu}g/ml$), Dictyopteris prolifera ($IC_{50}$, 16.66 ${\mu}g/ml$), Rugulopteryx okamurae ($IC_{50}$, 50.63 ${\mu}g/ml$), and Petrospongium rugosum ($IC_{50}$, 101.62 ${\mu}g/ml$). Furthermore, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay showed no cytotoxicity on mouse pre-adipocytes cell line (3T3-L1). These results suggest that some edible macroalgae merit further evaluation for clinical usefulness as anti-diabetic functional foods.