• 한국응용곤충학회지
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• 제57권1호
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• pp.33-38
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• 2018

호두는 경제적으로 중요한 유실수 중 하나이지만, 호두나무 포장 내에서 해충에 의한 피해 특성에 대한 정보는 매우 부족하다. 본 연구에서는 복숭아명나방과 굴피가는나방에 의한 호두열매와 잎의 피해를 조사하였다. 2017년 충남 부여군에 위치한 포장에서 복숭아명나방에 의한 호두열매 피해율의 변화를 조사하였다. 또한 김천, 부여, 영동 및 화성의 포장 내에서 2종의 나방에 의한 호두열매 및 잎의 피해율을 조사하였다. 부여 포장 내 호두열매의 피해율은 시간이 흐름에 따라 지속적으로 증가하였고, 피해율은 늦여름에 가장 높았다. 호두열매의 최종 피해율은 부여, 김천 및 화성에서 각각 22.1%, 20.5%, 11.7%로 조사되었다. 굴피가는나방에 의한 잎 피해는 성목의 신초 가지(평균 11.2%)에 비해 묘목의 신초 가지(평균 58.5%)에서 더 높은 것으로 조사되었다. 본 연구 결과, 호두나무 포장 내에서 복숭아명나방과 굴피가는나방에 의한 열매와 잎 피해가 심각한 것으로 확인되었으며, 향후 이들에 대한 방제기술 개발이 필요할 것으로 판단된다.

• The Plant Pathology Journal
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• 제35권1호
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• pp.11-18
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• 2019

Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. $TUB{\beta}$ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of $TUB{\beta}$ expression. The expression profile of ExoPG assessed using $TUB{\beta}$ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.

• The Plant Pathology Journal
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• 제37권5호
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• pp.437-445
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• 2021

Tilletia laevis Kuhn (syn. Tilletia foetida (Wallr.) Liro.) causes wheat common bunt, which is one of the most devastating plant diseases in the world. Common bunt can result in a reduction of 80% or even a total loss of wheat production. In this study, the characteristics of T. laevis infection in compatible wheat plants were defined based on the combination of scanning electron microscopy, transmission electron microscopy and laser scanning confocal microscopy. We found T. laevis could lead to the abnormal growth of wheat tissues and cells, such as leakage of chloroplasts, deformities, disordered arrangements of mesophyll cells and also thickening of the cell wall of mesophyll cells in leaf tissue. What's more, T. laevis teliospores were found in the roots, stems, flag leaves, and glumes of infected wheat plants instead of just in the ovaries, as previously reported. The abnormal characteristics caused by T. laevis may be used for early detection of this pathogen instead of molecular markers in addition to providing theoretical insights into T. laevis and wheat interactions for breeding of common bunt resistance.

• The Plant Pathology Journal
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• 제35권3호
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• pp.208-218
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• 2019

Here, we reported a novel secreted protein elicitor PeBL2 from Brevibacillus laterosporus A60, which can induce hypersensitive response in tobacco (Nicotiana benthamiana). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein expressed in Escherichia coli induced the typical hypersensitive response (HR) and necrosis in tobacco leaves. Additionally, PeBL2 also triggered early defensive response of generation of reactive oxygen species ($H_2O_2$ and $O_2{^-}$) and systemic resistance against of B. cinerea. Our findings shed new light on a novel strategy for biocontrol using B. laterosporus A60.

• Journal of Forest and Environmental Science
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• 제36권2호
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• pp.173-174
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• 2020

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2005년도 한국식물병리학회 임시총회 및 춘계학술발표회
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• pp.68.1-68.1
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• 2005

• Journal of Applied Biological Chemistry
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• 제50권3호
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• pp.173-174
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• 2007

• International Journal of Industrial Entomology and Biomaterials
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• 제3권1호
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• pp.97-99
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• 2001

Seven vegetables were planted in green houses to compare their yields and damages by insect pests based on three treatments, green music (GM), ultra sonic (US) and control. The vegetable yields of GM treatment were increased by an average of 17.81% than that of control for two years. There was a remarkable statistic difference in the yield between GM and respective controls such as radish, young radish, cucumber, spinach and Brassica oleracea var. acephala. There was no notable yield difference between US and controls for the semen vegetables within two years. The population densities of the insect pests in GM and US treatments were lower than controls. Only a few insect pests of some vegetables in GM and US treatments injured more seriously than in control.

• The Plant Pathology Journal
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• 제39권2호
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• pp.234-234
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• 2023

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2005년도 춘계 학술발표회
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• pp.121-121
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• 2005