• Journal of Ginseng Research
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• 제20권2호
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• pp.173-178
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• 1996

Aerobic cells are normally protected from the damage of free radicals by antioxidative on , zymes such as superoxide dismutase (SOD), catalase, glutathione (GSH) peroxidase, GSH S- transferase and GSH reductase which scavenge free radicals as well as nonenzymatic antioxidants such as ceruloplasmin, albumin and nonprotein-SH including GSH. The effects of each component (ginsenoside $Rb_1$, $Rb_2$, Rc, Rd, Re, $Rb_1$, Rf, $Rh_1$ and $Rh_2$) of red ginseng on the antioxidative enzyme activities were investigated in the liver in order to screen antioxidative components of red ginseng. Ginsenoside $Rb_1$ and Rc showed a tendency to increase GSH peroxidase activity, while ginsenoside Rc significantly decreased Cu,Zn-SOD activity. Especially, ginsenoside $Rh_2$ significantly increased catalase activity. These results suggest that ginsenoside $Rh_2$ is an important active component among total saponins of red ginseng.

• Bulletin of the Korean Chemical Society
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• 제22권4호
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• pp.362-366
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• 2001

To show the effects of metallothionein (MT) on the activity of enzymes involved in the removal of reactive oxygen species, MT has been added to the assay systems of superoxide dismutase (SOD), catalase and peroxidase. We have used assay systems of SOD based on NADPH oxidation and nitrite formation from hydroxylammonium chloride as an assay of superoxide breakdown rate. The two assay systems showed different results at the high concentration of MT. MT showed the scavenging of superoxide in the SOD assay system in the presence and absence of SOD. MT added to the SOD assay system behaved as an activator of SOD, but apo-MT behaved as an inhibitor. When MT was added to the assay system in the presence of a fixed amount of SOD, the breakdown rate of superoxide increased. The effects of MT on the decomposition of hydrogen peroxide and the activity of catalase and peroxidase decomposing hydrogen peroxide were evaluated. MT decreased the activities of catalase and peroxidase. We have concluded that the function of MT as an antioxidant might effect the level of superoxide scavenging and not the level of hydrogen peroxide.

• KSBB Journal
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• 제6권1호
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• pp.27-33
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• 1991

감자(Solannum tuberosum) 괴경에 A. rhizogenes를 접종하여 암소에서 배양한 결과 2-4주 후에 hairy root가 유도되었다. 유도된 hairy root는 opine 분석을 통해 agropine과 manopine이 검출되어 형질이 전환되었음을 확인하였으며, 발생이 진행됨에 따라 단백질은 전차 감소 되었고, 전분은 점차 증가함을 보였으며, GS.II의 활성 또한 발생단계에 따라 증가함을 보였다. $Ca^{2+}$ channel blocker 인 verapamil 처리구에서는 약 60% 정도 GS II의 활성이 감소 되었으며, calmodulin inhibitor인 chloropromazine 처리구에서는 GS II의 활성에 별 영향을 미치지 않았다. 한편 peroxidase 활성은 3주 동안 배양한 callus에서 가장 높았으며, peroxidase 양상은 hairy root에서는 pI 5.4, 5.9의 band가 나타난 반면, 3주 동안 배양한 callus에서는 pI 6.4, 7.1, 7.4의 새롱룬 band를 얻었다.

• 한국식품영양과학회지
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• 제24권5호
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• pp.651-657
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• 1995

모유성분을 고분자분획(20KD 이상 분자)과 저분자분획(20KD 이하 분자)으로 나누어 항산화력을 검토한 결과, 고분자분획이 저분자분획 보다 더 강력한 항산화력을 나타내었다. 이 모유의 항산화력은 주로 20KD 이상에 존재하는 항산화 효소에 기인할 가능성이 시사되어 모유의 채유 기간에 따른 항산화 효소를 비롯하여 protein-SH와 nonprotein-SH를 검토한 결과, catatase는 7일째 현저히 저하한데 비해 GSH peroxidase, GSH S-transferase는 7일째를 전후로 현저히 증가한 후 감소하는 경향을 나타내었다. Protein-SH는 채유 기간에 따라 차츰 감소하였으나 nonprotein-SH는 20일째 peak를 이루고 그 후 감소하였다. 이상의 결과로 부터 모유의 고분자분획은 강력한 항산화력을 나타내었으며, 이 항산화력은 catalase, GSH peroxidase 및 GSH S-transferase 등의 항산화 효소 활성에 기인할 것으로 사료된다.

• 미생물학회지
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• 제45권1호
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• pp.82-85
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• 2009

내분비장애물질은 분해가 매우 어려워 자연계에서 먹이그물을 통하여 사람에게 농축 전달된다. 이들은 정상적인 내분비계에 혼란을 일으키며, 특히 성호르몬의 작용에 많은 피해를 준다. 이를 효율적으로 분해하고 이들의 에스트로겐 활성을 제거하고자 백색부후균의 하나인 아교버섯(Phlebia tremellosa)을 활용하여 4가지 내분비계 장애물질의 분해에 대한 실험을 수행하였다. 아교버섯의 manganese peroxidase (MnP) 활성을 높이기 위하여 구름버섯의 MnP 유전자를 아교버섯에 도입하여 형질전환체를 확보하였으며 이들은 유전적으로 MnP 활성을 안정되게 나타냈다. 내분비 장애물질을 분해하는 조건에서 내분비장애 물질에 따라 30${\sim}$45%의 분해율을 보인 야생형에 비하여 이 형질전환체들 중 T5는 70${\sim}$88%의 분해율을 보였으며 에스트로겐 활성의 제거에도 약 2배 향상된 능력을 보였다.

• Journal of Microbiology and Biotechnology
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• 제12권6호
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• pp.972-978
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• 2002

Streptomyces sp. AD001 is a Gram-positive soil actinomycetes secreting an uncharacterized 2,4-dichlorophenol (DCP) oxidizing enzyme, whose activity is similar to the previously known Actinomycetes lignin-peroxidase (ALiP). This extracellular peroxidase was purified from Streptomyces sp. AD001 as a single protein band on an SDS-PACE by ammonium sulfate fractionation, Q-sepharose, concanavalin A, and Bio-Gel HTP column chromatographies. The molecular mass of the purified peroxidase was determined by SDS-PAGE to be 45.2 kDa, and 49.7 kDa with MALDI-TOF-MS, respectively. The highest level of peroxidase activity was observed at pH 7.5 and $30^{\circ}C$. The amino terminal sequence of the purified peroxidase (G-E-P-E-E-G-N-V-D-G-T-L) showed no significant homologies to my known proteins, suggesting that Streptomyces sp. AD001 may secrete a novel kind of bacterial peroxidase Initial rate kinetic data of the 2,4-DCP oxidation were best modeled with a random-binding bireactant system.

• 한국환경농학회지
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• 제3권2호
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• pp.37-42
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• 1984

화학물질(化學物質)에 의(依)한 서양고추냉이와 사과의 peroxidase 및 정제된 대두(大豆) lipoxygenase 역가저해(力價沮害)를 조사(調査)하였다. 서양고추냉이와 사과의 peroxidase 역가(力價)에 저해효과(沮害效果)를 주는 농약중(農藥中) maneb가 zineb의 효과(效果)보다 컸으며 10,20,30ppm의 처리농도(處理濃度)에서 maneb는 12.5,23.0,34.5%, zineb는 9.5, 17.5,27.5%의 역가저해(力價沮害)를 보였다. Maneb와 zineb의 혼합체(混合體)인 mancozeb 를 500 ppm의 농도(濃度)로 사과나무에 철포(撤布)했을때 사과의 peroxidase 역가변화(力價變化)는 무처리구(無處理區)와 별 차이(差異)가 없었다. 대두(大豆)의 lipoxygenase 역가(力價)에 미치는 영향(影響)은 zineb의 경우 가장 저해도(沮害度)가 컸으며 phosphamidon과 carbofuran 이 또한 저해효과(沮害效果)가 있었다. 그러나 maneb는 저해효과(沮害效果)가 없었다. 10, 20, 30 ppm의 처리농도하(處理濃度下)에서의 lipoxygenase 역가저해도(力價沮害度)는 zineb가 14.0, 27.0, 40.0%, carbofuran이 6.5, 12.5, 20.0% 그리고 phosphamidon이 4.5, 9.0, 13.0%였다. 또한 이들 세농약(農藥)은 $100{\sim}200\;ppm$에서 100% lipoxygenase 역가저해(力價沮害)를 보였다.

• 한국환경성돌연변이발암원학회지
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• 제21권2호
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• pp.67-71
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• 2001

Effects of exposure to a neurotoxicant, fenitrothion on antioxidant enzyme activities in Chironomus riparius Mg. (Diptera, Chironomidae) larvae were evaluated under laboratory conditions. Exposure to this chemical led to an increase of cupper, zinc type superoxide dismutase and manganese type superoxide dismutase activities and to a decrease of glutathion peroxidase activity. An activation of catalase was observed in the larvae exposed to high fenitrothion concentration. The response of superoxide dismutase was rapid and sensitive to low chemical concentrations, but changes in catalase, total peroxidase and glutathion peroxidase were less sensitive. In this study, antioxidant enzyme activities in Chironomus riparius larvae were identified as pertinent biomarkers for environmental monitoring.

• BMB Reports
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• 제32권2호
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• pp.168-172
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• 1999

We previously reported that a novel thiol peroxidase (p20) from Escherichia coli is a distinct periplasmic peroxidase that detoxifies hydroperoxides together with glutathione or thioredoxin. Until now, there was no experimental evidence for the presence of thioredoxin (Trx) in the periplasmic space. In an attempt to confirm the physiological function of p20 as a thiol peroxidase supported by Trx in the periplasmic space, we have purified a Trx activity from the periplasmic space of Escherichia coli and identified the Trx as the same protein as the cytoplasmic Trx. The presence of Trx in the periplasmic space of Escherichia coli suggests that p20 is a unique extracellular Trx-linked thiol peroxidase.

• 한국식품영양과학회지
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• 제19권3호
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• pp.224-228
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• 1990

Cabbage contained high peroxidase activity among tested plant sources. The cabbage peroxi-dase can replace horseradish peroxidase to assay glucose with glucose oxidase. The amount of glucose can be determined quantitatively by glucose oxidase-cabbage peroxidase. The opti-mum pH and temperature for enzymatic glucose determination by glucose oxidase-cabbage peroxidase were 6.0 and 35-45$^{\circ}C$ respectively. The glucose assay was inhibited by addition of various metal salts such as mercuric chloride lead acetate silver nitrate ammonium molyb-date sodium tunstate and cupric sulfate. The relationship between absorbance and amount of glucose was linear up to 8.33 mM glucose in the assay mixture under the assay conditions.