Inhibition of Peroxidase and Lipoxygenase by Pesticides

Peroxidase와 Lipoxygenase에 대한 농약의 저해작용

The effect on the activities of peroxidase and lipoxygenase by chemicals was determined. Peroxidase activities of horseradish and apple were inhibited strongly by maneb and comparativelyby mancozeb, zineb. The inhibitory rate of enzyme activity was ranged in $12.5{\sim}34.5%$ by maneb, $11.0{\sim}30.5%$ by mancozeb, and $9.7{\sim}27.5%$ by zineb in the level of $10{\sim}30\;ppm$ at the final concentration. No signification was in the peroxidase activity of apple during ripening between control and plot, treated with 500 ppm mancozeb. On the activity of purified soybean lipoygenase, zineb inhibited it more strongly than carbofuran or phosphamidon, but maneb did not have inhibitory effect on that. The inhibition ranges of $14.0{\sim}40.0%$ by zineb, $6.5{\sim}20.0%$ by carbofuran and $4.5{\sim}13.0%$ by phosphamidon were shown in the final pesticide concentration of $10{\sim}30$ ppm. But in $100{\sim}200$ ppm, lipoxygenase activity was almost inhibited by carbofuran and phosphamidon.

화학물질(化學物質)에 의(依)한 서양고추냉이와 사과의 peroxidase 및 정제된 대두(大豆) lipoxygenase 역가저해(力價沮害)를 조사(調査)하였다. 서양고추냉이와 사과의 peroxidase 역가(力價)에 저해효과(沮害效果)를 주는 농약중(農藥中) maneb가 zineb의 효과(效果)보다 컸으며 10,20,30ppm의 처리농도(處理濃度)에서 maneb는 12.5,23.0,34.5%, zineb는 9.5, 17.5,27.5%의 역가저해(力價沮害)를 보였다. Maneb와 zineb의 혼합체(混合體)인 mancozeb 를 500 ppm의 농도(濃度)로 사과나무에 철포(撤布)했을때 사과의 peroxidase 역가변화(力價變化)는 무처리구(無處理區)와 별 차이(差異)가 없었다. 대두(大豆)의 lipoxygenase 역가(力價)에 미치는 영향(影響)은 zineb의 경우 가장 저해도(沮害度)가 컸으며 phosphamidon과 carbofuran 이 또한 저해효과(沮害效果)가 있었다. 그러나 maneb는 저해효과(沮害效果)가 없었다. 10, 20, 30 ppm의 처리농도하(處理濃度下)에서의 lipoxygenase 역가저해도(力價沮害度)는 zineb가 14.0, 27.0, 40.0%, carbofuran이 6.5, 12.5, 20.0% 그리고 phosphamidon이 4.5, 9.0, 13.0%였다. 또한 이들 세농약(農藥)은 $100{\sim}200\;ppm$에서 100% lipoxygenase 역가저해(力價沮害)를 보였다.