• Title/Summary/Keyword: Permeability, ${\mu}$

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Phosphorylation of AQP4 Water Channel Regulates Water Permeability (Aquaporin 4 water channel 인산화에 의한 수분 투과도의 조절)

  • 박권희;정동근;정진섭;이재숙;예운해;서덕준;배혜란
    • Journal of Life Science
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    • v.10 no.5
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    • pp.456-466
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    • 2000
  • Aquaperin 4 (AQP4) is the mercurial water channel expressed abundantly in brain, especially the region related with cerebrospinal fluid reabsorption and osmoregulation. The primary structure of AQP4 water channel was elucidated but the molecular mechanism of AQP4 channel regulation is still unknown. To investigate the possible regulation of AQP4 water channel by phosphorylation via various protein kinases, osmotic water permeability of AQP4 expressed in Xenopus oocytes was measured by videomicroscopy technique. Forskolin (10 $\mu$M) did not affect osmotic water permeability of oocytes injected with AQP4 cRNA, excluding the regulation of AQP4 water cnannel by protein kinase A. Osmotic water permeability (P아래첨자) of AQP4-expressed oocytes was ingibited by the pretreatmeat of BAPTA/AM (up to 500$\mu$M), an intracellular Ca윗첨자 chelator, and calmidazolium (100$\mu$M), a specific Ca윗첨자/calmodulin antagonist, in a dose-dependent manner. The inhibition of osmotic water permeability (P아래첨자) by the calmidazolium treatment was completely reversed by the addition of calyculin A (0.1$\mu$M), a nonspecific phosphatase inhibitor. Phorbol 12-myristate 13-acetate (PMA), a protein kinase C activator, had biphasic effects on osmotic water permeability in AQP4 cRNA injected oocytes depending on its concentration; 21% increase by 100 nM PMA, 35% decrease by 1$\mu$M PMA. These effects were reversed with 2$\mu$M staurosporine, a nonspecific PKC inhibitor. These results suggest that phosphorylation of AQP4 water channel by Ca윗첨자/calmodulin kinase and protein kinase C might regulate the osmotic water permeability.

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The Effects of Microstructures and Some Additives (CoO and $Al_2O_3$) on the Magnetic Properties of Mn-Zn Ferrite (미세조직 및 첨가성분 (CoO와 $Al_2O_3$)이 Mn-Zn Ferrite의 자기적 성질에 미치는 영향)

  • 변수일;장승현
    • Journal of the Korean Ceramic Society
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    • v.16 no.3
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    • pp.142-154
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    • 1979
  • The effects of microstructures and some additives $(CoO and Al_2O_3$) on the magnetic properties such as initial permeability, $\mu$-T curve, coercive force, and magnetic induction of MnZn ferrites have been studied. The powder was prepared by Hot Petroleum Drying Method. The basic composition of MnZn ferrites was 25.5mole % MnO, 22.0 mole% ZnO, 52.5 mole% $Fe_2O_3$. CoO in a concentration range from 0.05 to 0.5 mole% and $Al_2O_3$ from 2.5 to 7.5 mole% were added. Sintered density increased up to 97.5% of theoretical density. Permeability increased as average grain size increased, and that coercive force decreased as average grian size increased. Magnetic induction increased as sintered density increased. The variation of initial permeability with temperature in a temperature range from 0$^{\circ}$ to $60^{\circ}C$ was lowered (a flatter $\mu-T$ curve) as sintering temperature decreased. The compensation temperature To ofmagnetocrystalline anisotropy constant K1 and initial permeability varied with the species and amount of additives. When 0.05 mole% CoO was added to the basic composition, initial permeability at $15^{\circ}C$ increased from 5200 to 5900. The variation ofinitial permeability with temperature in a temperature range from 0^{\circ}to $60^{\circ}C$ was smaller (a flatter $\mu$-T curve) than that of the basic composition of Mn Zn ferrites. When 2.5 mole% $Al_2O_3$ was added, initial permeability at $15^{\circ}C$ decreased from 5200 to 3000. But the variation of initial permeability with temperature in a temperature range from 0$^{\circ}$to $60^{\circ}C$ was smaller (a flat ter $\mu-T$ curve) than when 0.05 mole% CoO was added. Experimental results showed that the conditions necessary for the occurrence of a very high permeability and a flat $\mu$-T curve were controversial even in a temperature range from $0^{\circ}$to $60^{\circ}C$.

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Measurement of Longitudinal Liquid Permeability Using Pressure Bomb Method (Pressure Bomb법을 이용한 섬유방향 액체투과성 측정)

  • Hur, Jong-Yun;Kang, Ho-Yang
    • Journal of the Korean Wood Science and Technology
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    • v.25 no.3
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    • pp.66-74
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    • 1997
  • Liquid permeabilities of red oak and several softwoods were measured by the pressure bomb method and a modified liquid permeability method in order to investigate their efficacy. The effect of preboiling and prefreezing on wood permeability were also examined for both green and resaturated specimens. Regardless of some disadvantages these two methods were revealed as a handy tool for quick evaluation of the permeability of an unknown species. The permeabilities of the resaturated specimens increased when preboiled. but decreased when prefrozen. For green specimens, however, pre freezing increased permeability. The discrepancy of the pre freezing effect on two specimens partially attributes to their difference of initial permeabilities. For all species except radiata pine heartwood, the radii of the effective capillary pores, derived from the water potential equation, distribute from $0.42{\mu}m$ to $7.2{\mu}m$. Those of radiata pine heartwood are below $0.46{\mu}m$.

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Air Permeability of Softwoods Imported from the Russian Far East (북양(北洋) 침엽수재(針葉樹材)의 공기(空氣) 투과성(透過性))

  • Jee, Woo-Kuen;Kim, Gyu-Hyeok
    • Journal of the Korean Wood Science and Technology
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    • v.24 no.4
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    • pp.22-31
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    • 1996
  • Air permeabilities for both sapwood and heartwood of the three softwoods(Picea jezoensis, Larix gmelini, Pinus sylvestris), imported from the Russian Far East, in the three different structural directions were measured using steady-state rotameter method. After measuring permeability, the liquid absorption into the longitudinal permeability specimens was measured, and then related with the measured permeability. The longitudinal permeability was overwhelmingly greater than the transverse permeability, with the radial direction showing higher permeability than the tangential direction. There appear to be species differences in permeability of the three softwoods. The mean longitudinal sapwood permeability of P. jezoensis(3.300darcy) and P. sylvestris(3.028darcy) were considerably greater(ca. 25times) than that of L. gmelini(0.134darcy). Also the average longitudinal heartwood permeability of P. jezoensis(0.300darcy) was about 10 times as great as that of P. sylvestris(0.029darcy) and L. gmelini(0.024darcy). The average calculated radius of pit openings in the heartwood was $0.101{\mu}m$ for L. gmelini, $0.152{\mu}m$ for P. sylvestris for and $0.703{\mu}m$ for P. jezoensis. Heartwood permeability increases with the increase in radius of pit pore and the decrease in ring width and in extractive content, even though the correlation between permeability and its affecting factors was dependent on species. However, there was an inverse relationship between specific gravity and permeability, showing poor correlation between them. The correlation between air permeability and liquid retention was fairly high, so it was revealed that prediction of liquid absorption into the wood by using permeability was feasible.

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The Effect of the Thermal Stress on the Transport Property of Pocheon Granite (열응력이 포천화강암의 투수성에 미치는 영향)

  • Yoon, Yong-Kyun
    • Tunnel and Underground Space
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    • v.7 no.3
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    • pp.238-245
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    • 1997
  • A transient pulse methos has been used to measure the permeability of Pocheon granite pre-heated from $25^{\circ}C$ to $600^{\circ}C$ at effective pressure up to 32MPa. The permeability of whole rock ranged from 0.72 $\mu$d at 10MPa to 0.20 $\mu$d at 32MPa. The permeability of rock heated to $600^{\circ}C$ ranged from 18.07$\mu$d at 10MPa to 6.39$\mu$d at 32MPa. Confining pressure has greater effects on the rocks thermally treated to lower thermal-cycle temperatures than on the higher thermally treated rocks. The increase of permeability is most pronounced between 40$0^{\circ}C$ and $600^{\circ}C$. Below 40$0^{\circ}C$, permeability increase is expected to be associated with the formation of new cracks and widening of preexisting cracks, whereas above 40$0^{\circ}C$, permeability increase is expected to reflect widening of cracks. Using the equivalent channel model, author shows that the exponent n in the relationship relating the permebility(k) to porosity($\phi$) by k∝$$\phi$^n$ falls in the range 2.7$\leq$n$\leq$3.0.

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Variation of the Relaxation Time for NiCuZn Ferrites with Magnetic Properties

  • Nam, Joong-Hee;Oh, Jae-Hee
    • Journal of Magnetics
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    • v.1 no.1
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    • pp.37-41
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    • 1996
  • The frequency dependence of complex permeability for various NiCuZn ferrites was investigated. The variation of complex permeability for NiCuZn ferrites can be presented as a form of a semi-circle, so called the Cole-Cole plot, and the relaxation phenomena were explained with various shapes of the plots. The relaxation time $\Upsilon$ was calculated from $f_rx$, which is a relaxation frequency at ${\mu"}_{max}$. Relations between anisotropy field $H_A$ and relaxation time $\Upsilon$, initial permeability $\mu_i$ and $H_A$ were plotted to identify the frequency dependence of complex permeability.lity.

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The Properties of Permeability and Ash-Removal of Sintered Fail Safety Filter of Dust Collector in High Temperature (고온 집진용 Fail Safety 소결 필터의 통기도와 분진제거 특성)

  • Bae S-Y;Ahn I-S;Jung W-H;Choi J-H
    • Korean Journal of Materials Research
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    • v.14 no.7
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    • pp.470-476
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    • 2004
  • The fail safety filter is an assistant filter element to be mounted in order to intercept the particles leaked when the main filter elements are broken. So it should have two contrary functions of being plugged easily to meet the purpose of dust sealing and a high permeability to save the space. The permeability of the metal filter elements were effectively controlled by the following factor: powder size(53-840 ${\mu}m$) and applied pressure(1000-2000 $kgf/cm^2$), and then the compact were sintered for 1 hour at $1200^{\circ}C$ in vacuum sintering furnace. The sintered metal filters was evaluated for the function of the fail safety filter in an experimental unit. The maximum allowable particle size was 420-840 ${\mu}m$, when a CIP pressure of 1500 $kgf/cm^2$ was applied reveals a permeability of about $1.2{\times}10^{10}m^2$ and pore size of about 60 ${\mu}m$. The metal filter produced with stainless steel powder of 480-840 ${\mu}m$ size, which presented excellent permeability than commercial ceramic filter element and plugged with in 3 minutes with the leak of the maximum particle size less than 3 ${\mu}m$.

Preparation of Ceramic Foam Filter and Air Permeability (집진용 세라믹 필터의 제조 및 공기 투과 특성)

  • 박재구
    • Journal of Korean Society for Atmospheric Environment
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    • v.16 no.4
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    • pp.381-388
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    • 2000
  • Ceramic foam prepared with cordierite as a starting material by foam method was tested to evaluate the feasibility as a filter for the dust collection in hot gas. Two different types of agents Benzethonium chloride (BZTC, C27H42NO2Cl) and Sodium Lauryl Sulfate(SLS, CH3(CH2)11OSO3Na) were used as foaming agents in foaming process. Porosityof ceramic foam was about 80% and mean pore size were 100${\mu}{\textrm}{m}$ for SLS agent and 200 ${\mu}{\textrm}{m}$ for BZTC. It was observed that ceramic foam was composed of continuous macro-pore structure with opening windows interconnecting macro-pores. The surface of ceramic foam support of was coated with cordierite particles ranged from 20${\mu}{\textrm}{m}$ to 50${\mu}{\textrm}{m}$ Meso-pore size in the coating layer on ceramic foam was below 10${\mu}{\textrm}{m}$. While air permeability of the support increased with increasing macro-pore size coated ceramic filters showed a constant permeability without regard to the macro-pore size of the support. The permeabuilities of support varied in the range of 600$\times$10-13m2 to 1000$\times$10-13m2. For the case of coated ceramic filter it was about 200$\times$10-13m2. As a result of particle trapping test by using fly ash the particle removal efficiency was over the 99.9%.

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The Effect of Chemical Vapor Infiltrated SiC Whiskers on the Change in the Pore Structure of a Porous SiC Body

  • Joo, Byoung-In;Park, Won-Soon;Choi, Doo-Jin;Kim, Hai-Doo
    • Journal of the Korean Ceramic Society
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    • v.43 no.4 s.287
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    • pp.199-202
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    • 2006
  • In this study, SiC whiskers were grown on a porous SiC diesel particulate filter for nanoparticle filtering. To grow the whiskers at the inner pore without closing the pores, we used chemical vapor infiltration with a solution source and a dilute. As the deposition time increased, the whiskers grew and formed a network structure. After 180 min of deposition, the mean diameter of the whiskers was 174 nm and the compressive strength was 58.4 MPa. The pores shrank from $10{\mu}m\;to\;0.4{\mu}m$ and, because the whiskers filed the inner pores, the gradient of permeability decreased as the deposition time increased. However, by using the network structure of whiskers deposited for 120 min and 180 min, we obtained a diesel particulate filter with pores of $0.98{\mu}m\;and\;0.4{\mu}m$, respectively. Furthermore, the filter shows better permeability than a porous body with pores of $1{\mu}m$. In short, by filtering the nanoparticulate materials, the network structure of whiskers improves the strength, reduces the pore size and minimizes the permeability drop.

Skin Permeability of Porcine Placenta Extracts and Its Physiological Activities

  • Han, JeungHi;Kim, Mi-Ryung;Park, Yooheon;Hong, Yang Hee;Suh, Hyung Joo
    • Food Science of Animal Resources
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    • v.33 no.3
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    • pp.356-362
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    • 2013
  • We investigated the skin permeability and various biological activities of porcine homogenate of placenta (HP) with the highest protein contents (452.89 ${\mu}g/mg$). The content of protein in subcritical extract of HP (SPE) was decreased from the initial content of 452.9 ${\mu}g/mg$ to 262.7 ${\mu}g/mg$ at 3 h subcritical extract. The contents of amino type nitrogen (A-N) were sharply increased from 35.1 ${\mu}g/mg$ of initial content to 305.9 ${\mu}g/mg$ at 3 h subcritical extract. The HP showed a noticeable activity in terms of antioxidant capacity for ferric reducing antioxidant power (FRAP) assay and especially for 2,2'-Azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) method. HP, SPE-0.5, SPE-2 and SPE-3 showed inhibitory effect on elastase activities with an $IC_{50}$ of 46.1, 42.9, 31.6 and 34.7 ${\mu}g/mL$, respectively. SPEs showed more significantly inhibitory effect than HP (p<0.05). The skin permeability of the SPEs was higher than that of the HP. SPE-3 showed highest skin permeation and the permeability was significantly higher than that of HP. SPE-2 also showed significantly higher permeation than HP after 4 h. As expected, increase of extraction time significantly increased skin permeability in the subcritical extract of HP (SPE). From these results, in terms of cost and source availability, porcine placenta extracted with subcritical extraction has advantages over untreated PE and have potential as a cosmetic ingredient.