• Journal of Periodontal and Implant Science
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• 제45권3호
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• pp.101-110
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• 2015

Purpose: Sclerostin, an inhibitor of Wnt/${\beta}$-catenin signaling, exerts negative effects on bone formation and contributes to periodontitis-induced alveolar bone loss. Recent studies have demonstrated that serum sclerostin levels are increased in diabetic patients and that sclerostin expression in alveolar bone is enhanced in a diabetic periodontitis model. However, the molecular mechanism of how sclerostin expression is enhanced in diabetic patients remains elusive. Therefore, in this study, the effect of hyperglycemia on the expression of sclerostin in osteoblast lineage cells was examined. Methods: C2C12 and MLO-Y4 cells were used in this study. In order to examine the effect of hyperglycemia, the glucose concentration in the culture medium was adjusted to a range of levels between 40 and 100 mM. Gene expression levels were examined by quantitative reverse transcription-polymerase chain reaction and Western blot assays. Top-Flash reporter was used to examine the transcriptional activity of the ${\beta}$-catenin/lymphoid enhanced factor/T-cell factor complex. Tumor necrosis factor-alpha ($TNF{\alpha}$) protein levels were examined with the enzyme-linked immunosorbent assay. The effect of reactive oxygen species on sclerostin expression was examined by treating cells with 1 mM $H_2O_2$ or 20 mM N-acetylcysteine. Results: The high glucose treatment increased the mRNA and protein levels of sclerostin. High glucose suppressed Wnt3a-induced Top-Flash reporter activity and the expression levels of osteoblast marker genes. High glucose increased reactive oxygen species production and $TNF{\alpha}$ expression levels. Treatment of cells with $H_2O_2$ also enhanced the expression levels of $TNF{\alpha}$ and sclerostin. In addition, N-acetylcysteine treatment or knockdown of $TNF{\alpha}$ attenuated high glucose-induced sclerostin expression. Conclusions: These results suggest that hyperglycemia increases sclerostin expression via the enhanced production of reactive oxygen species and $TNF{\alpha}$.

• Journal of Periodontal and Implant Science
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• 제42권5호
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• pp.173-178
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• 2012

Purpose: The main purpose of this study was to investigate bone thickness on the buccal and palatal aspects of the maxillary canine and premolars using cone-beam computed tomography (CBCT). The differences between left- and right-side measurements and between males and females were also analyzed. Methods: The sample consisted of 20 subjects (9 males and 11 females; mean age, $21.9{\pm}3.0$) selected from the normal occlusion sample data in the Department of Orthodontics, The Catholic University of Korea. The thickness of the buccal and palatal bone walls, perpendicular to the long axis of the root were evaluated at 3 mm and 5 mm apical to cemento-enamel junction (CEJ) and at root apex. Results: At the canines and first premolars regions, mean buccal bone thickness of at 3 mm and 5 mm apical to CEJ were less than 2 mm. In contrast, at the second premolar region, mean buccal bone thickness at 3 mm and 5 mm apical from CEJ were greater than 2 mm. Frequency of thick bone wall (${\geq}2mm$) increased from the canine to the second premolar. Conclusions: This result should be considered before tooth extraction and planning of rehabilitation in the canine and premolar area of maxilla. Careful preoperative analysis with CBCT may be beneficial to assess local risk factors and to achieve high predictability of success in implant therapy.

• Journal of Periodontal and Implant Science
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• 제41권3호
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• pp.149-156
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• 2011

Purpose: Globular adiponectin (gAd) is a type of adipocytokine, which is mainly produced by adipose tissue. It has been reported that gAd acts as a pro- as well as an anti-inflammatory factor. Interleukin (IL)-6 and IL-8 are pro-inflammatory cytokines. To investigate the role of gAd on periodontal tissues, the expression of adiponectin receptor 1 (AdipoR1) and the effect of gAd on the expression of IL-6 and IL-8 were investigated in periodontal ligament (PDL) and gingival fibroblasts. Methods: PDL and gingival fibroblasts were cultured from human periodontal tissues. gAd derived from Escherichia coli and murine myeloma cells were used. The expression of AdipoR1 was estimated by reverse transcription-polymerase chain reaction and western blot The expression of cytokines was measured by enzyme-linked immunosorbent assay. Results: PDL and gingival fibroblasts expressed both mRNA and protein of AdipoR1. gAd derived from E. coli increased the production of IL-6 and IL-8, but polymyxin B, an inhibitor of lipopolysaccharide (LPS), inhibited IL-6 and IL-8 production induced by gAd in both types of cells. gAd derived from murine myeloma cells did not induce IL-6 and IL-8 production in those cells. gAd derived from E. coli contained higher levels of LPS than gAd derived from murine myeloma cells. LPS increased production of IL-6 and IL-8 in PDL and gingival fibroblasts, but pretreatment of cells with gAd derived from murine myeloma cells did not inhibit LPS-induced IL-6 and IL-8 expression. Conclusions: Our results suggest that PDL and gingival fibroblasts express AdipoR1 and that gAd does not act as a modulator of IL-6 and IL-8 expression in PDL and gingival fibroblasts.

• 구강회복응용과학지
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• 제30권1호
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• pp.1-8
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• 2014

목적: 이 연구는 파노라마방사선사진 촬영 시 환자의 머리 위치가 상방, 하방, 좌측 및 우측으로 회전됨에 따른 수평 및 수직 확대율의 변화를 비교 평가하는 것이었다. 연구 재료 및 방법: 직경 4 mm인 금속구 5개를 건조 두개골의 하악 전치부(2개)와 우측 대구치부(3개)의 치조골 상방에 위치시켰다. 상하 및 좌우 회전량을 조절할 수 있는 두개골 고정장치에 건조 두개골을 고정시킨 후, 기준 위치와 회전된 위치에서 파노라마방사선사진을 쵤영하였다. 결과: 기준 위치에서 평균 수평 확대율은 $1.22{\pm}0.01-1.44{\pm}0.01$이었고, 평균 수직 확대율은 $1.29{\pm}0.00-1.35{\pm}0.02$였다. 전치부와 구치부 비교시 수평 확대율은 전치부에서 $1.24{\pm}0.02-1.31{\pm}0.03$, 구치부에서 $1.40{\pm}0.03-1.33{\pm}0.02$로 유의한 차이가 있었고(P < 0.05), 수직 확대율은 차이가 없었다. 위치 변화에 따른 수평 확대율은 $0.88{\pm}0.03-3.73{\pm}0.16$으로 기준 위치와 비교 시 통계적으로 유의한 차이를 보인 반면 (P < 0.05), 수직 확대율은 $1.24{\pm}0.01-1.37{\pm}0.02$로 차이가 없었다. 결론: 회전된 머리위치에서 파노라마방사선사진 촬영 시 수평 확대율의 변화가 크다.

• 구강회복응용과학지
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• 제23권4호
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• pp.327-336
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• 2007

Fixture의 안정도를 평가하는 장비인 Osstell Mentor와 기존 Osstell과의 비교 시험 및 측정환경에 따른 노이즈 발생 시험 결과를 통해 다음과 같은 결과를 얻었다. 1. US II Fixture와 SS II Fixture의 ISQ 값을 측정한 1차 검증에서는 Osstell과 Osstell Mentor의 ISQ 값 경향의 차이가 크게 나타나지 않음을 확인하였다. 2. SS II Fixture를 얕게 매식한 것과 정상적인 매식한 것의 ISQ 값 비교를 한 2차 검증에서는, Osstell과 Osstell Mentor 모두 매식 깊이에 따른 동일한 경향의 결과를 보였다. 3. 3차 검증에서는 골질별로 Osstell과 Osstell Mentor의 ISQ 값이 일정한 경향을 보이는 것을 확인하였고, 특히 Osstell Mentor 장비는 Osstell 장비보다 사용함에 있어서 편리성이 뛰어나다고 판단된다. 또한 ISQ 값을 100% 신뢰하기는 어렵지만 Osstell Mentor를 임상 및 실험에 적용하는데 있어서는 문제가 없을 것으로 판단된다. 4. 측정환경에 따라 전자파를 발생시키는 환경이 존재하면 노이즈가 발생할 수 있으나, 실제 측정결과 휴대폰이 작동 중인 환경을 제외하고는 노이즈가 발생하지 않았다. 실제 치과 내에서의 사용에서도 노이즈는 발생하지 않음을 확인하였다. 5. 비교적 안전하게 장치를 장착 및 탈착 할 수 있어 측정과정에 의해 발생되는 임플란트와 주위골에 대한 잠재적 위험도가 낮고 측정위치에 따른 오차가 적어 임상적으로 불리한 상황과 다양한 환경에서의 임플란트 안정성 측정이 가능해 임상적 활용도가 높다고 판단된다.

• 구강회복응용과학지
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• 제30권2호
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• pp.131-137
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• 2014

목적: 이 연구는 다양한 거칠기를 가진 알루미늄 옥사이드 연마석으로 치주기구 날 세우기를 시행시 연마석의 거칠기에 따른 치주기구 날 세우기의 효율성 및 마모도를 비교 평가하였다. 연구 재료 및 방법: 30개의 No. 9 - 10 그레이시 큐렛이 사용되었고, 모든 큐렛은 치석제거술 및 치근활택술을 시행함으로써 날을 무디게 만들고 3가지 그룹(240, 600, 800 grit)으로 임의로 나누었다. 치주기구의 예민도는 연마석을 최대 20회 기구에 적용한 후 평가되었고, 마모도는 최대 80회 후 기구 날의 끝 1 mm와 2 mm에서 기구 날의 폭으로 평가되었다. 결과: 치주기구 예민도는 5회와 10회 상하 운동시 240 grit에서 유의하게 높게 나타났으며(P < 0.001), 15회, 20회에서는 유의한 차이는 없었다(P > 0.05). 마모도는 240 grit의 경우 600 grit과 800 grit에 비해 약간 높게 나타났지만 통계적 유의성은 없었다. 결론: 알루미늄 옥사이드 연마석의 입자가 클수록 치주기구 날 세우기의 효율성은 증가하였다. 하지만 기구의 마모에 유의해야 한다.

• 구강회복응용과학지
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• 제29권3호
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• pp.249-258
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• 2013

이 연구의 목적은 환자의 머리 위치에 따른 파노라마방사선사진의 수평 및 수직 확대율을 비교 평가하는 것이었다. 직경 4 mm인 금속구를 건조 두개골의 하악 전치부와 우측 대구치부의 치조골 상방에 위치시켰다. 수평 및 수직적 위치의 변화를 재현하기 위해 수직, 수평 이동량을 조절할 수 있는 두개골 고정장치를 이용하여, 전방, 후방, 좌측 및 우측으로 이동시킨 후 파노라마방사선사진을 OP-100D를 이용하여 획득하였다. 촬영된 영상은 DICOM 형식으로 저장되었고, INFINITT PACS software를 이용하여 금속구의 폭과 높이의 평균값을 구하였다. 적절한 위치에서 촬영된 파노라마방사선사진에서 금속구의 수평 확대율은 1.224-1.439였고, 수직 확대율은 1.286-1.345였다. 건조 두개골의 위치 변화에 따른 수평 확대율은 0.798-6.297로 통계적으로 유의한 차이를 보인 반면(P<0.05), 수직 확대율은 1.245-1.418 정도로 수평 확대율에 비해 차이를 보이지 않았다.

• Imaging Science in Dentistry
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• 제42권3호
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• pp.175-182
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• 2012

Purpose: The aim of this study was to provide sex-matched three-dimensional (3D) statistical shape models of the mandible, which would provide cephalometric parameters for 3D treatment planning and cephalometric measurements in orthognathic surgery. Materials and Methods: The subjects used to create the 3D shape models of the mandible included 23 males and 23 females. The mandibles were segmented semi-automatically from 3D facial CT images. Each individual mandible shape was reconstructed as a 3D surface model, which was parameterized to establish correspondence between different individual surfaces. The principal component analysis (PCA) applied to all mandible shapes produced a mean model and characteristic models of variation. The cephalometric parameters were measured directly from the mean models to evaluate the 3D shape models. The means of the measured parameters were compared with those from other conventional studies. The male and female 3D statistical mean models were developed from 23 individual mandibles, respectively. Results: The male and female characteristic shapes of variation produced by PCA showed a large variability included in the individual mandibles. The cephalometric measurements from the developed models were very close to those from some conventional studies. Conclusion: We described the construction of 3D mandibular shape models and presented the application of the 3D mandibular template in cephalometric measurements. Optimal reference models determined from variations produced by PCA could be used for craniofacial patients with various types of skeletal shape.

• Journal of Periodontal and Implant Science
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• 제43권4호
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• pp.153-159
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• 2013

Purpose: The level of the implant above the marginal bone and flap design have an effect on the bone resorption during the healing period. The aim of this study is to detect the relationship between the level of the implant at the implant placement and the bone level at the healing period in the mesial and distal side of implants placed with flapless (FL) and full-thickness flap (FT) methods. Methods: Twenty-two nonsubmerged implants were placed with the FL and FT technique. Periapical radiographs were taken of the patient at implant placement, and at 6 and 12 weeks. By using computer software, bone level measurements were taken from the shoulder of the healing cap to the first bone implant contact in the mesial and distal side of the implant surface. Results: At 6 weeks, the correlation between the crestal bone level at the implant placement and crestal bone level of the FT mesially was significant (Pearson correlation coefficient=0.675, P<0.023). At 12 weeks, in the FT mesially, the correlation was nonsignificant (Spearman correlation coefficient=0.297, P<0.346). At 6 weeks in the FT distally, the correlation was nonsignificant (Pearson correlation coefficient=0.512, P<0.107). At 12 weeks in the FT distally, the correlation was significant (Spearman correlation coefficient=0.730, P<0.011). At 6 weeks in the FL mesially, the correlation was nonsignificant (Spearman correlation coefficient=0.083, P<0.809). At 12 weeks in the FL mesially, the correlation was nonsignificant (Spearman correlation coefficient= 0.062, P<0.856). At 6 weeks in the FL distally, the correlation was nonsignificant (Spearman correlation coefficient=0.197, P<0.562). At 12 weeks in the FL distally, the correlation was significant (Pearson correlation coefficient=0.692, P<0.018). Conclusions: A larger sample size is recommended to verify the conclusions in this preliminary study. The bone level during the healing period in the FT was more positively correlated with the implant level at implant placement than in the FL.

• 대한소아치과학회지
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• 제33권2호
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• pp.304-310
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• 2006

본 연구는 치주질환 주요 병인균주 중의 하나인 P. intermedia를 대상으로 하여, 이 균주에서의 hemin 결합 단백질 유전자를 분리하고 염기서열을 결정하기 위하여 수행되었다. 본 연구에서는 약 5,000개의 recombinant colony들을 스크리닝하여 hemin 결합 단백질 유전자를 포함하고 있는 것으로 여겨지는 1개의 클론(pHem1)을 확인하였다. Restriction enzyme mapping 결과 pHem1의 insert DNA의 크기는 약 2.5kb이었으며 P. intermedia chromosomal DNA 내에는 hemin 결합 단백질 유전자가 single copy로 존재하였고, transcript의 크기는 약 1.8kb이었다. 분리한 pHem1 유전자는 1개의 ORF를 가지고 있으며, ORF의 크기는 2,550bp로 약 850개의 아미노산 폴리펩타이드로 구성되어 있다. 또한, pHem1 유전자는 이미 밟혀진 다른 유전자들과 상동성을 보이지 않았다. 본 연구는 P. intermedia에서의 porphyrin생리 및 hemin 획득기전을 분자생물학적으로 규명하는데 있어 중요한 의의가 있으리라 사료된다. 향후 pHem1 유전자의 특성 분석 등이 수행되어야 할 것으로 여겨진다.