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Possibility of Repeated Use of Elite Donor Cows for Mass Production of OPU-Derived Embryos (OPU 유래 수정란의 대량생산을 위한 고능력 공란우 반복사용 가능성에 관한 연구)

  • Jin, Jong-In;Choi, Byung-Hyun;Kim, Seong-Su;Park, Bun-Young;Lee, Jung-Gyu;Kong, Il-Keun
    • Journal of Embryo Transfer
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    • v.30 no.3
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    • pp.149-159
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    • 2015
  • This study was designed to know the possibility in repeat uses of elite donor cows for getting mass production of OPU-derived embryo production (OPU-IVP). Ultrasound transvaginal ovum pick-up (OPU) performed in 6 Korean native cows was aged 4 to 10 years old. The aspiration of immature oocytes for OPU derived embryo was carried out 2 times per week, and OPU-IVP of $1^{st}$ period was carried out 22~48 sessions from each donors. And the break time for OPU-IVP of $2^{nd}$ period after $1^{st}$ OPU from each donors were 2~25 months. The OPU-IVP of $2^{nd}$ period each donors conducted total 15~65 times for 2~8 months by an ultrasonographic, was guided follicular aspiration system. The average numbers of collected oocytes, grade 1 + grade 2(G1+G2) oocytes and cleavage embryo from $1^{st}$ period OPU-IVP were significantly differences between donors (p<0.05). Total collected oocytes of donor D were significantly higher compared with donors of A, B, C, E and F (average 17.0 per session vs. 11.2, 10.1, 8.5, 10.2 and 9.6; p<0.05) and also oocytes of G1+G2 were significantly higher compared with r A and D and subsequently to donors of B, C, E and F (average 7.9 and 8.5 per session vs. 5.0, 2.7, 6.0 and 1.6; p<0.05). Cleavage rate of donor D was significantly higher compared with donors of A, B, C, E and F (average 13.1 per session vs. 10.1, 9.1, 6.9, 8.9 and 6.7; p<0.05). The average numbers of OPU-IVP for $1^{st}$ period was significantly higher from donors of B, D and E than those from donors of A, C and F (average 6.5, 7.1 and 6.5 per session vs. 3.5, 4.2 and 2.8; p<0.05). The possibility investigation of $2^{nd}$ OPU-IVP was carried out after 2~25 months rest periods from $1^{st}$ period OPU session. Total average numbers of collected oocytes, cleavages and blastocyst development rates were significantly higher from $1^{st}$ period OPU compared with $2^{nd}$ period one (p<0.05). The OPU-IVP efficiency by break for more embryo production from elite cow was analysis comparing without rest of donor A, under 6 months rest period as B and over 6 months rest period as C and then the average numbers of collected oocytes, cleavages and blastocysts were significantly higher from A group (11.8, 9.5 and 5.2 per session) than those from B and C groups (7.9, 6.2 and 2.6 vs. 9.2, 7.5 and 3.9, p<0.05), and also C group was significantly higher than B group. In conclusion, $1^{st}$ period OPU-IVP was more efficient compared with $2^{nd}$ period repeated uses of donor, and the break times for additional production of embryo on donor were needed more than over 6 months after $1^{st}$ period OPU-IVP. This repeating uses of elite donor cows given more emphasis for getting the opportunity on mass production of elite cow OPU-IVP embryo should be increased G1+G2 possibility of genetic improvement of livestock within short period.

A Study on the Effect of Water Level and Inundation Period on the Plant Coverage of Revegetation Methods of Stream Bank in the Coastal Reclaimed Land - Focus on a Case of the Test-bed of the Simgok Stream in Incheon - (해안매립지 하천의 수위 및 침수기간이 저수하안 식생공법의 식생피도에 미치는 영향 - 인천광역시 심곡천 실험구의 사례를 중심으로 -)

  • Shin, Beom-Kyun;Kim, Hyea-Ju;Choi, Gye-Woon;Han, Man-Shin
    • Korean Journal of Environment and Ecology
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    • v.26 no.3
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    • pp.437-445
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    • 2012
  • The study performed the implementation monitoring of the revegetation methods applied to the test-bed of Simgok Stream in Incheon to identify the effect of water level and inundation period on the vegetation coverage of the stream bank revegetation methods. The categories of monitoring included the plant species and plant coverage for each method, physicochemical property of soil, water level and water quality. The result of monitoring revealed that plant growth conditions of all revegetation methods of each stream bank were good in the first survey as of May 2010. However, in the second survey of June 2011 when inundation period was less than a week, plant growth conditions and coverage of revegetation methods were partially bad. In the third survey as of August 2011 when inundation period was longest as 8 weeks during survey period, most vegetations did not survive except for Phragmites communis. But plant species number and plant coverage were increased gradually in the forth survey as of October 2011 when inundation period was less than 2 weeks so water level decreased more than that of third survey. Accordingly, the correlation analysis among number of plant species and plant coverage on stream bank, which applied revegetation method, water level and inundation period was performed for quantitative analysis. The result revealed that number of plant species and plant coverage has a negative correlation with water level and inundation period, but inundation period had higher correlation with plant occurrence than water level.

A Study on Improving Pitch Search for Vocoder (보코더에서 피치검색 성능개선에 관한 연구)

  • Baek, Geum-Ran;Bae, Myung-Jin
    • The Journal of the Acoustical Society of Korea
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    • v.31 no.7
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    • pp.419-426
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    • 2012
  • The pitch searching is a vital process in a vocoder. Generally, the method of pitch searching is employed after highlighting the periodicity, where a correlation is identified with the signal by changing the interval of two pulses. When the correlation value reaches the peak, the pitch can be found by the pulse interval because it is the repetition interval with most striking period. However if the identified period happens to be one of half period, double period or triple period, this cannot be considered as the pitch period. Many methods were suggested to solve this problem. An inaccurate pitch could be obtained as well, when there is an interval where signal amplitude is not constant but varies abruptly in the frame. To solve this matter, searching the pitch by dividing a frame into various subframes is adopted, but too much calculation has to be followed while it leads the correct value. This paper suggests an algorithm to resolve these two problems. First, to search the pitch after advance correction of the signal energy level with an estimated overall energy change ratio in the frame before pitch search to reduce half period, double period and triple period is suggested. Second, to vary the number of subframes by predicting the amplitude change rate in the frame by the energy ratio obtained by the above-mentioned method is advised. If these two methods are applied, the pitch searching time can be reduced and the general pitch searching performance can be improved without affecting the sound quality in the synthesized signal.

STATISTICAL STUDY ON HABITUAL METHODS Of TOILET TRAINING FOR INFANT AND TODDLER (한국 영.유아의 대소변 가리기 훈련습성에 관한 일 연구)

  • 최경자
    • Journal of Korean Academy of Nursing
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    • v.3 no.3
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    • pp.119-132
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    • 1973
  • This study observed on habitual methods of toilet training for infants and toddlers in Korea thru 300 mothers at four medical institutions Ewha Woman′s University Hospital, Ewha Maternal and Child Health Center, Severance Hospital and Seoul National University Hospital) with a design to analyze beginning time, duration of period, methods employed, motives, equipments used, special terminology used for the subject training. The main purpose of this study was to generalize the proper methods of toilet training, and also to contribute a better psychological education for the mother and child. The results obtained from this study were as follows; 1. Majority of (67.3%) observed were in the age category between 30 and 40 years, and dominant numbers (64.7%) were housewives with high level of education and from middle class family background. 2. In the most cases (85%), toilet training was carried out by mothers(including wives who had a job) while more than half of mothers (53.7%) maintained their important motive for the training was "due to having high regard for cleanliness" 3. As for the time of beginning toilet training: finding indicated that starting period was decided (70%) at inconsiderate desertion of each mother. whereas, only minority group (30%) represents the cases where mother started the training when they consider tile child was physically and psychologically randy. Also greater number (77.7%) started bladder training prior to that of bowel. 4. It is noticeable that in course of training a large number of mothers (48.3%) applied strict training method when the child proper talenting, and the more rigid and strict in tile training. the more malformation of personality of the infant and toddler were seen after the training period (P<0.01). 5. Over the half of the total cases denoted (bowel 54.3%, bladder 67.7%) starting period before one year and in most cases (bowel 79.3%, bladder 729)the training was accomplished within 12 month, and therefore it was noted that earlier start(before 1 year) shortened the training period (with 12 month) . There was no significant difference between male and female infants in both starting period and duration of period in bladder training, however, in bowel training there was a tendency that female started earlier(7-12 months needed, 51.4%) than tile male (13-18 months needed, largest number 41.4%), and also in cases of female the period for needed for training were shorter than the cases of male. 6. Many a number (bowel 50.3%, bladder 97.7%) employed the method of continuous talenting at regular interval in accordance with that of child′s habit formed before training. Equipment used were various kinds, however, pieces of paper for male (45.5%) and piss pot or bedpost (42.3%) for female were common, on the other hand, "Eung-ga" for defecation (52.3%) and "Shii" for the urination (95.3%) were most standard expression that used during the training period.

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Development of Model for Optimal Concession Period in PPPs Considering Traffic Risk (교통량 위험을 고려한 도로 민간투자사업 적정 관리운영기간 산정 모형 개발)

  • KU, Sukmo;LEE, Seungjae
    • Journal of Korean Society of Transportation
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    • v.34 no.5
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    • pp.421-436
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    • 2016
  • Public-Private-Partnerships tend to be committed high project development cost and recover the cost through future revenue during the operation period. In general, long-term concession can bring on more revenue to private investors, but short-term concession less revenue due to the short recovering opportunities. The concession period is usually determined by government in advance or by the private sectors's proposal although it is a very crucial factor for the PPPs. Accurate traffic forecasting should be most important in planing and evaluating the operation period in that the forecasted traffic determines the project revenue with user fees in PPPs. In this regards, governments and the private investors are required to consider the traffic forecast risk when determining concession period. This study proposed a model for the optimal concession period in the PPPs transportation projects. Monte Carlo simulation was performed to find out the optimal concession period while traffic forecast uncertainty is considered as a project risk under the expected return of the private sector. The simulation results showed that the optimal concession periods are 17 years and 21 years at 5.5% and 7% discount level, respectively. This study result can be applied for the private investors and/or any other concerned decision makers for PPPs projects to set up a more resonable concession period.

The Diurnal Change of Accommodative Functions by Near Work (일과 중 근거리 작업에 따른 조절기능의 변화)

  • Lee, Jeong-Yun;Yu, Dong-Sik;Son, Jeong-Sik;Cho, Hyun Gug;Moon, Byeong-Yeon
    • Journal of Korean Ophthalmic Optics Society
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    • v.16 no.1
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    • pp.75-81
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    • 2011
  • Purpose: The daily changes of accommodation at period 1 (08:00~10:00), period 2 (13:00~15:00) and period 3 (18:00~20:00) were surveyed with fifty two incipient presbyopia of from 38 to 49 years old. Methods: 52 incipient presbyopia subjects (30 men, 22 women) were classified into 4 groups (groupI: ${\geq}$ 7 hrs~group IV: < 3 hrs) according to near working amount. Their amplitude of accommodation, accommodative facility and relative amplitude of accommodation were inspected, respectively. Results: The amplitude of accommodation was measured to be the lowest for the groupI that near working time was the most during daily work, and the amplitude of accommodation of oculus dexter, oculus sinister and oculus uterque were the highest at period 2 in case of all the group I~IV. In the accommodative facility, it appeared that the oculus dexter, oculus sinister and oculus uterque of group I, oculus dexter and oculus sinister of group II, and oculus dexter, oculus sinister and oculus uterque of group III were the highest at period 2. The oculus uterque of group II and oculus dexter, oculus sinister and oculus uterque of group IV were the highest accommodative facility at period 3. It appeared that the negative relative accommodation was the highest at period 1 in case of all the group I~IV, and the positive relative accommodation was the highest at period 2 in case of the group I~III and was the highest at period 3 in case of the group IV. Conclusions: Because the difference of near working time affects the accommodation, it would become a help in solving the asthenopia symptom of the incipient presbyopia that opticians consider their near working time during daily work and take a checkup on their accommodation.

A Study on Diurnal Variations of Accommodation (일과 중 조절기능 변화에 관한 연구)

  • Lee, Jeong-Yun;Yu, Dong-Sik;Son, Jeong-Sik;Moon, Byeong-Yeon
    • Journal of Korean Ophthalmic Optics Society
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    • v.15 no.1
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    • pp.73-78
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    • 2010
  • Purpose: The purpose of this study was to examine diurnal variation of accommodation on people whose age are between 35 and 50 years old with frequent alternation of accommodation at period 1 (8:00-10:00), period 2(13:00-15:00) and period 3 (18:00-20:00). Methods: All \04 subjects were separated to nonpresbyopia and incipient presbyopia. Tests were done for amplitude of accommodation, accommodative facility and relative amplitude of accommodation. Results: Both nonpresbyopia and incipient presbyopia had the highest amplitude of accommodation in oculus dexter, oculus sinister and oculus uterque at period 2. In addition, the incipient presbyopia showed the statistical significance of daily change. The nonpresbyopia had the most accommodative facility at period 3 and incipient presbyopia had the most accommodative facility at period 2. Their daily variations were statistically significant. Both nonpresbyopia and incipient presbyopia had the most negative relative accommodation of daily change at period I. Both of them had the most positive relative accommodation at period 2. Conclusions: The incipient presbyopia had the highest amplitude of accommodation, accommodative facility. and positive relative accommodation (except the negative relative accommodation) at period 2.

The Effect of Level and Period of Fe-methionine Chelate Supplementation on the Iron Content of Boiler Meat

  • Seo, S.H.;Lee, H.K.;Lee, W.S.;Shin, K.S.;Paik, I.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.10
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    • pp.1501-1505
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    • 2008
  • A broiler experiment was conducted to compare the effects of duration and level of iron-methionine chelate (Fe-Met) supplementation on the iron, copper (Cu) and zinc (Zn) content of broiler meat. Two hundred and fifty hatched Ross broiler chickens were randomly assigned to 5 dietary treatments. Each treatment had 5 replicates of 10 birds (5 males and 5 females) each. Birds were housed in raised floor batteries and fed traditional broiler diets ad libitum for 5 weeks. Dietary treatments were as follows: Control and two levels of Fe-Met (100 or 200 ppm in Fe) supplemented for either the whole period (0-5 wk) or grower period (4-5 wk). Production performance was not significantly affected by treatments. Iron content in the muscles (breast, leg and wing) and organs (liver and spleen) were significantly (p<0.05) increased as the level and duration of Fe-Met supplementation increased. The highest concentration of iron was shown in Fe-Met 200 fed for the whole period. Liver contained the highest amount of iron followed by spleen, leg muscle, wing muscle and breast muscle. Supplementation of Fe-Met 200 for the grower period resulted in higher iron concentration in liver and spleen than supplementation of Fe-Met 100 for the whole period. However, the same treatment resulted in lower iron concentration in muscles (breast, leg and wing) than the treatment of Fe-Met 100 for the whole period. In order to achieve the highest iron enrichment in the muscles, Fe-Met should be supplemented at 200 ppm in Fe for the whole period (5 wks). Fe-Met supplementation increased copper concentration in all muscles and organs except wing muscle. Zinc concentration decreased in breast and wing muscle but tended to increase in leg muscle, liver and spleen by Fe-Met 200 supplementation. Color of muscle was not significantly affected by Fe-Met treatments. However, redness of leg and breast muscle, and yellowness of leg and breast muscle tended to increase by supplementation of Fe-Met for the whole period. It was concluded that iron content of broiler meat can be effectively enriched by supplementation of 200 ppm of Fe as Fe-Met for 5 wks.