• Journal of Microbiology and Biotechnology
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• v.20 no.8
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• pp.1243-1250
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• 2010

A cDNA encoding a cysteine protease inhibitor (CPI) was isolated from the cDNA library of clamworm Perinereis aibuhitensis Grube. The deduced amino acid sequence analysis showed that the protein had 51%, 48%, and 48% identity with Zgc:153129 from Danio rerio, cystatin B from Theromyzon tessulatum, and the ChainA, stefin B tetramer from Homo sapiens, respectively. The gene was cloned into the intracellular expression vector pET-15b and expressed in Escherichia coli. The recombinant CPI (PA-CPI) was purified by affinity chromatography on Ni-charged resin and ion-exchange chromatography on DEAE-Sepharose FF. The relative molecular mass of PA-CPI was 16 kDa as deduced by SDS-PAGE. Activity analysis showed that the recombinant protein could inhibit the proteolytic activity of papain. A constitutive and secretive expression vector was also constructed, and the cDNA encoding CPI was subcloned into the vector for extracellular expression. Western blotting analysis results showed that the PA-CPI was secreted into the medium. Bioassay demonstrated that E. coli DH5${\alpha}$ harboring pUC18ompAcat-CPI showed a significant difference in mortality to the Asian longhorned beetle Anoplophora glabripennis compared with untransformed E. coli DH5${\alpha}$ and control.

• Ocean Science Journal
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• v.40 no.4
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• pp.221-227
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• 2005

The architecture of macrofaunal burrows and the total area of the sediment-water interface created by biogenic structure were investigated in the Donggeomdo tidal flat on the west coast of Korea. Resin casting methods were applied to recover burrows of four dominant species, Macrophthalmus japonicus, Cleistostoma dilatatum, Perinereis aibuhitensis, and Periserrula leucophryna, and whole burrows within the casting area at three sites in different tidal levels. P. leucophryna excavated the largest burrow in terms of a surface area among them. In the case of whole burrow casting, the space occupied by the biogenic structure was extended into deeper and expanded more greatly at the higher tidal level. In the uppermost flat, the burrow wall surface area within sediment was more extensive than the sediment surface area. Increased oxygen supply through the extended interface could enhance the degradation rates of organic carbon and also change the pathways of degradation. Quantifying the relationship between the extended interface and mineralization rate and pathway requires more extensive study.

• Journal of Marine Life Science
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• v.7 no.2
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• pp.113-120
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• 2022

Despite concerns about the significant toxicity of copper pyrithione (CuPT) at environmental concentrations, effects of CuPT on benthic organisms have received little attention. Here, we analyzed the detrimental effects of CuPT at sublethal concentrations (1/50, 1/20, and 1/10 of the 96 h-LC50 value) for 14 days in the marine polychaete Perinereis aibuhitensis. Reduced burrowing activity and significantly decreased the acetylcholinesterase activity in response to relatively high concentrations of CuPT were identified as CuPT-triggered cholinergic inhibition. The lipid peroxidation marker, malondialdehyde levels were dose-dependently increased, whereas intracellular glutathione was depleted by relatively high concentrations. In the CuPT-treated polychaete, significant fluctuations in the enzymatic activities of the antioxidant defense system (catalase, superoxide dismutase, glutathione reductase, and glutathione peroxidase) were observed with significantly modulated glutathione 𝘚-transferase activity. These results indicate that even sublethal levels of CuPT would have detrimental effects on the health status of the marine polychaete.

• Ocean and Polar Research
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• v.24 no.1
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• pp.63-71
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• 2002

This preliminary study on the changes of macrobenthic assemblages in experimental sediment fences was conducted as a part of tidal marsh restoration project. Intertidal sediment fences were designed to increase the efficiency of trapping sediments on unvegetated tidal flats in order to raise sediment elevation and to allow colonization of intertidal vegetation. Although increment of soil surface level was not observed over the first three months of the study, it was possible to obtain some effects of the sediment fence. Three months later, the particle sizes of the surface sediment at experimental plots became much finer compared to unfenced areas on the natural mudflats located in the same tide level as that of the plots. The difference was much greater on the plot with drainage canals than on the plot without ones. Species diversity of the experimental plots became much higher than that of natural sites. Perinereis aibuhitensis and Glauconome chinensis which were absent from initial community appeared with high density in the plot with drainage canals. Those species were significantly different in abundance between the experimental plot and the natural mudflat. Changes in species composition were not detected in another experimental plot without drainage canals.

• Journal of fish pathology
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• v.22 no.1
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• pp.15-21
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• 2009

White spot syndrome virus (WSSV) is a strong causative agent for high mortality in cultured and wild shrimps. From this study, the WSSV prevalence in marine organisms around shrimp farm as well as live feed-mediated transmission of WSSV to farmed shrimps were investigated. Based on nested-PCR method, WSSV was detected in wide array of marine organisms including Perinereis aibuhitensis (81.3% of prevalence rate, 13/16), Enedrias fangi (100%, 16/16), Ruditapes philippinarum (20%, 2/10), crab larvae (100%, 10/10), copepoda (30%, 3/10), Periophthalmus modestus (50%, 5/10), Pachygrapsus crassipes (10%, 1/10), Helice tridens (20%, 2/10) and Neomysis sp. (70%, 7/10). On the other hand, WSSV was not detected in Bullacta exarata, Uca arcuata, and Reishia clavigera. The percent prevalence of WSSV in wild shrimps, Fenneropenaeus chinensis was only 6%, but markedly increased up to 56% after a feeding trial using polychaete worms for one month, indicating that the live feed is one of significant carriers of WSSV to shrimps under practical farming conditions.

• Korean Journal of Environment and Ecology
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• v.16 no.1
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• pp.34-45
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• 2002

Of the four tidal flat areas(Yeocha-ri, Dongmak-ri, Sundu-ri and Donggum-ri) on Southern Ganghwa Island, the distribution of migratory waterbirds showed a difference in the numbers of individuals and species. Higher numbers of individuals and species were observed on the Yeocha-ri tidal flat. The dominant waterbirds species at the four areas was the Dentin(Calidris alpina). This species showed an occupancy rate(42.0-79.1%) on the Yeocha-ri tidal flat, which was much higher relative to the other areas. The Yeocha-ri tidal flats were higher relative to MSL than the other tidal flats. When the Dongmak-ri tidal flat was covered by sea water, the Yeocha-ri tidal flat was 0.0317$\textrm{km}^2$ in area and on this space we observed many resting waterbirds. The biomass of the waterbirds' potential prey on the Yeocha-ri tidal flat was higher(1.6~2.5$\times$) than other tidal flats. Twenty-three species and 266 individuals were found on the Yeocha-ri tidal flat, which was higher than the tidal flats. The food of Dunlin, which was the dominant species in these four areas, was mainly Reticunassa festiva and Umbonium thomasi(Gastropoda) and Perinereis aibuitensis(Polychaeta). A Dunlin(38.11g) used to eat 1,295 individuals of these food items for daily basal energy. Gastropod, its density was highest on Yeocha-ri tidal flats. The distribution of waterbirds seemed to be closely related with the distribution of the benthos, space and safety of resting site. We suggest that the environments of tidal flats, which are rich foraging habitats, are important factors for conservation of waterbirds.