• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.12 no.4
• /
• pp.261-266
• /
• 1979

This experiment was carried out to evaluate the sanitary quality of cultured vegetables and to check the removing rate of bacteria by treating methods such as washing with tap water or commercial detergent, or blanching. Samples collected from farm land located at Busan suburbs and markets were Fragaria chiloensis var. ananasa, Lycopersicum esculentum, Capsium longum, Cucumis sativus, Lactuca scariola var. sativa leaf of Perilla frutescens var, japonica, Oananthe stolonifera and Allium odoium. Fecal coliform MPN was ra god from less than 30 to 430,000 per 100 grams of samples examined while plate count was $7.2\times10^3\;to\;2.2\times10^7$ per gram. Usually contamination rate of fecal coliform of leaf vegetal)les was much higher than that of fruit vegetables. Removing rate of bacterial density of vegetables by washing three times with tap water was about $70\%$ in fruit vegetables, about $20\%$ in leaf vegetables but it was about $80\%$ in leaf vegetables by washing with a commercial detergent. Sirvival rate of viable cell count of leaf vegetables was less than $0.1\%$ after blanching for one minutes in boiling tap water, Composition of coliform was $18\%$ Escherichia coli group, $22\%$ Citrobacter freundii group and $60\%$ Klebsiella aerogenes group, among Escherichia coli, type I being $16\%$ in total. The coliform detection from BGLB gas positive tubes being planted with various vegetables was about $80\%$ in the result of IMViC reaction.

• The Korean Journal of Mycology
• /
• v.43 no.3
• /
• pp.200-205
• /
• 2015

Perilla frutescens var. japonica Hara was collected from farmland in Seongju-gun. Fifteen endophytic fungal strains with different colony morphologies were isolated from roots of P. frutescens. Waito-c rice seedlings were treated with the concentrated culture filtrates (CF) of endophytic fungi for observation of their plant growth-promoting activities. In the results, the CF of Y2H001 fungal strain promoted the growth of the waito-c rice seedlings. The phylogenetic tree of Y2H001 strain was analyzed by the combined sequences of the partial internal transcribed spacer region (ITS) and partial betatubulin gene. Molecular and morphological studies identified the Y2H001 strain as belonging to Aspergillus flavus. In gas chromatography mass spectrometry (GC/MS) analysis of the CF of Y2H001 strain, gibberellic acid (GA) was detected and quantified. Therefore, we describe Y2H001 strain as a new $GA_3$-producing A. flavus based on morphological, molecular characteristics and analysis of secondary metabolite.

• Korean Journal of Food Science and Technology
• /
• v.24 no.6
• /
• pp.610-615
• /
• 1992

The difference in content and composition of total lipid, lipid fractions and fatty acids of perilla leaves being used fresh vegetable was investigated in relation to the color of undersurface, i.e. green perilla leaves(GPL) and violet perilla leaves(VPL), by column- and thin layer- and gas chromatography. Total lipid(TL) content was of little difference between green leaves(GPL) (5.24%dw) and violet one (VPL) (5.02%dw), while neutral lipid(NL) content was higher In VPL(36.4% of TL) than GPL(34.7%). The major components were sterol ester and hydrocarbons(58.5%) and trigylcerides(14.9%) in NL, $mono-(42{\sim}45%)$ and $di-(13{\sim}15%)$ galactosyl digylceride in glycolipids(GL) and phosphatidyl ethanolamine$(40{\sim}45%)$ and phosphatidyl glycerol(13%) in phospholipids(PL) for both GPL and VPL. The number of component was 10 in all three fractions. The similarity of component between GPL and VPL was in decreasing order of NL(r=1.00), GL(r=0.997) and PL(r= 0.968). Major fatty acids were linolenic $(62{\sim}64%)$, palmitic$(10{\sim}12%)$ and linolic$(9{\sim}10%)$ for TL, linolenic, palmitic, myristic(43, 15, 14%) for NL, linolenic, oleic, palmitic(79, 11, 8%) for GL and linolenic, linoleic, palmitic(36, 25, 23%) for PL. Unsaturated fatty acid percentage was higher only in GL of VPL than GPL. The similarity of fatty acid composition between GPL and VPL was least in PL and so it was among other fraction with PL.

• Korean Journal of Food Science and Technology
• /
• v.47 no.1
• /
• pp.103-110
• /
• 2015

In this study, we evaluated the fatty acid composition and physiological activities of oils extracted from eight types of seeds, pepper (Capsicum annuum L.), green tea (Camellia sinensis L.), perilla (Perilla frutescens var. japonica Hara), peanut (Arachis hypogaea L.), cotton (Gossypium indicum LAM.), sesame (Sesamum indicum L.), walnut (Juglans regia L.), and safflower (Carthamus tinctorius L.). The composition and quality analysis showed that the oils were potentially suitable for foo-grade applications. The composition analysis showed that the oils were mostly composed of unsaturated fatty acids including linoleic acid and oleic acid. In 3T3-L1 adipocytes, green pepper, perilla, and peanut seed oils inhibited lipid accumulation, and green pepper, perilla, peanut, sesame, walnut, and safflower seed oils induced leptin secretion. These results show that the inhibitory effect of edible seed oils on lipid accumulation, and induction of leptin secretion may be useful for obesity management.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.37 no.2
• /
• pp.231-238
• /
• 2008

Quality attributes of fresh-cut sesame leaf (Perilla frutescens var. japonica) as affected by hydrocooling and packing were investigated in terms of weight loss, respiration, vitamin C content, total chlorophyll content, microbial load and sensory properties during storage at 4 and $10^{\circ}C$. Fresh sesame leaf was trimmed and washed with cold water (1, $5^{\circ}C$) as well as tap water ($13^{\circ}C$) for 30 sec 3 times and then packaged in PP (polypropylene) film bag and PETE (polyethylene terephthalate) tray, and stored for 9 days at 4 and $10^{\circ}C$. Weight loss was decreased by washing and packing. Respiration rate was increased slowly over the storage at $4^{\circ}C$. Vitamin C content and total chlorophyll contents of sesame leaf packaged within PETE tray decreased gradually during storage. Hydrocooling and packing within PETE tray treatments resulted in approximately $1{\sim}2\;log\;CFU/g$ reduction of microbial load.

• Korean Journal of Environmental Agriculture
• /
• v.15 no.1
• /
• pp.46-69
• /
• 1996

The studies were conducted to obtain the basic information of the effects of pollutants on plant species and to select the plant species showing specific responses to the pollutants. For these purposes, paraquat, ammonium, and cadmium as a source of oxidative stress, nitrogen toxicity, and heavy metal toxicity respectively were treated to the plant species. Among the tested plants, Lamiaceae, Brassicaceae, and Caryophyllaceae were tolerant to paraquat, whereas Poaceae and Asteraceae were sensitive. Especially Mosla dianthera of Lamiaceae, Hemistepta lyrata and Aster pilosus of Asteraceae, and Paspalum thunbergii of Poaceae showed higher tolerance than others. Paraquat resistance was related with life style, overwintering capacity, so perennial and biennial species showed higher tolerance than annual species. In response to ammonium, Poaceae showed higher resistance while Fabaceae and Caryophyllaceae showed sensitiveness. Weed species having tolerance to ammonium were Echinochloa crus-galli var. praticola, Panicum dichotomiflorum, Setaria glauca, Chenopodium album, and Solanum nigrum, while Mosla dianthera, Arenaria serpyllifolia and Perilla frutescens var. japonica showed sensitiveness. In the response of plant species to cadmium, Digitaria sanguinalis, Amaranthus lividus showed higher resistance, whereas Galinsoga parviflora, Plantago asiatica, Ambrosia trifida, and Paspalum thunbergii showed sensitiveness. The injured degree on germination stage by pollutants did not related with injured degree on matured stage. During germination, the root elongation was more sensitive than shoot elongation by pollutants, paraquat, ammonium, and cadmium.