• ALGAE
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• 제20권1호
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• pp.25-30
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• 2005

To clarify some confusions concerning identification of the Korean Peridinium species, genotypic analysis was performed with their SSU rDNA sequences. PCR was used to amplify the partial SSU rDNA of Peridinium isolates collected from three different Korean waters (Juam, Sang-sa and Togyo Reservoirs). The PCR products were allowed directly to sequence, which revealed each 942 bp of rDNA sequence. Analyses of the rDNA sequences showed that all the Korean isolates had the same genotype (100% sequence homology), and they were nearly identical to a Japanese strain of P. bipes f. occultatum (NIES 364; 99.8% sequence similarity). The sequence-based comparisons could clearly resolve P. bipes f. occultatum isolated from three different Korean waters.

• 생태와환경
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• 제38권spc호
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• pp.1-7
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• 2005

To resolve some of the most pressing uncertainties of Peridinium identity, morphological characteristics of Korean Peridinium were examined using light and scanning electron microscopy. The Peridinium samples were collected from three different regions of Juam, Sang-sa and Togyo Reservoirs, when seasonal blooms occurred. Formula of the epithecal plate was recorded with 7 precingular, 3 intercalary, and 4 apical plates (4', 3a, 7"). An apical pore, 3 ${\sim}$ 5 ${\mu}m$ in size, was apparently present. The cingulum was easily observed under light microscope, and was considerably offset by about 15 ${\mu}m$ (2 ${\sim}$ 3 times per cingulum width). The sulcus was straight longitudinally and widened apparently towards the antapex. None of spine was found on the surface of the thecal plates on scanning electron micrographs. The average body length was 50.4 ${\mu}m$ with a range of 29 ${\sim}$ 63 ${\mu}m$. The geometric dimension, as designated to the body length:width ratio, was found from calculation to be 1.12 with a range of 1.00 ${\sim}$ 1.35, therefore, the cell was shown slightly elongated. Based on their morphology, the causative organisms of red tides in three different Korean waters were identified as P. bipes f. occultatum, which was reported for the first time in Korea.

• 환경생물
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• 제23권4호
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• pp.323-327
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• 2005

Two morphologically different species of Peridinium were frequently observed in natural samples collected monthly from Togyo Reservoir. One was previously identified as P. bipes Stein f. occultatum (Lindem.) Lef. (Ki and Han 2005), the other was remained uncertainly so far. We resolved their identity, based on the morphological observations with scanning electron microscopy. The formula of epithecal plate was recorded with 7 precingular, 3 intercalary and 4 apical plates (4', 3a, 7'). An apical pore was apparently present. The conspicuous morphology of the Peridinium was three spines on the hypothecal plates. The average body length and width were $33.6\;{\mu}m$ with a range of $26\~45{\mu}m$, and 25.7 with a range of $19\~31{\mu}m$, respectively; the cell was, therefore, shown slightly elongated. Based on their morphological characteristics, the causative organism was identified as P. aciculiferum (Lemm.), which was reported for the first time in Korean freshwater.

• ALGAE
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• 제23권2호
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• pp.115-118
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• 2008

Cells of the dinoflagellate Peridinium were frequently observed in water samples of Togyo reservoir, and some species were responsible for dense blooms. Recently, we could identify them as P. bipes f. occultatum Lindem. and P. aciculiferum Lemm., considering morphology (Ki et al. 2005a; Ki and Han 2005b): However, some unidentified Peridinium cells with different shapes and body sizes were found among the samples collected during early spring. Here we describe their morphological characteristics such as thecal plate and body size to characterize its taxonomic identity by morphological characters. The formula of epithecal plates was recorded as 4 apical, 2 intercalary and 7 precingular plates (i.e. 4’', 2a, 7’'’') and the epicone in an apical view was symmetric. An apical pore was easy to make out under a light microscope. No cingular displacement was observed. The average body size was 33 $\mu$m in length with a range of 26-36 $\mu$m, and average 26 $\mu$m in width with a range of 21-31 $\mu$m, respectively; the cell was, therefore, shown slightly elongated. This way we identified Peridinium umbonatum Stein, 1883 for the first time from Korean freshwaters.

• 생태와환경
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• 제39권2호통권116호
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• pp.271-283
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• 2006

섬 지역의 초기 담수과정에 있는 상수원 저수지 (절곡저수지)에서 수색 악화와 여과지 폐색의 원인을 분석하기 위해 2005년 8월에 식물플랑크톤을 포함한 다각적인 수환경 조사를 수행하였다. 저수지의 형태는 단순하였고, 평균 수심은 5.5 m로서, 상류에서 얕고 댐 부근에서 가장 깊었다. 환경요인 중에서 수평적 또는 수직적 차이가 큰 인자는 DO, Chl-a이었고,가장 작은 인자는 수온이었다. 수중 투명도는 0.6 ${\sim}$ 0.9 m범위(평균값 0.7 m)이었고, 탁도의 평균값은 9.3 NTU (8.0 ${\sim}$ l2.1 NTU범위)이었다. 후명도와 탁도의 증감요인은 생물 또는 비생물의 복한적인 영향으로 달 수 있었고 공간적인 차이도 탄영되었다. 수색이 탁한 것은 무기입자의 증가와 식물플랑크톤치 과대증식 영향이 주된 원인이었고, 여과지 폐색은 저수지로부터 식물플랑크톤의 과잉 공급에 의한 문제점이었다. 저수지 내 chlorophyll-a농도의 범위와 평균값은 상층에서 31.6 ${\sim}$ 258.9 ${\mu}g\;L^{-1}$, 123.6 ${\mu}g\;L^{-1}$, 저층에서 17.0 ${\sim}$ 37.4 ${\sim}$, 26.5 ${\sim}$이었다. Chlorophyll-a의 증가는 담수적조의 대발생 영향이었고, 주종은 와편모조류 Peridinium bipes f. occultatum이었다. Peridinium의 분포는 chlorophyll-a농도와 밀접한 관련성이 있었다. 담수적조의 현존량은 상류지역에서 $8.5\;{\times}\;10^3\;cell\;mL^L{-1}$로서 많았고, 댐부근의 하류지역($4.4\;{\times}\;10^2\;cell\;mL^L{-1}$)으로 갈수록 감소하는 양상을 보였다. 또한, 담수적조의 원인종으로 규조류 Synedra acus와 남조류 Microcystis aeruginosa도 소량관찰되었다. 저수지의 수질 부영양화 현상은 기존 경사사면형 농경지를 기반으로 조성되었으므로 저층으로부터 풍부한 영양환경을 내재하고 있었고, 담수 초기에 발생할 수 있는 가능성을 포함하고 있었다. 또한 유입수량의 부족과 현재 수질개선을 위해 적용하고 있는 수중폭기시설등의 물리적인 영향도 직 ${\cdot}$ 간접적으로 작용하였을 것으로 추정되었다 따라서 향후 이에 대한 중장기적인 모니터링이 필요하였고 유역과 저수지의 통합적인 수질관리계획 이 요구되었다.