• Asian-Australasian Journal of Animal Sciences
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• 제8권5호
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• pp.433-441
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• 1995

A series of experiments was conducted to determine the influence of various pepsin-HCL pretreatment factor, hereby the factors of duration of washing for the retrieved bags, inherent to the mobile nylon bag technique (MNBT), on apparent ileal digestibility of crude protein (AIDCP) and apparent ileal digestibility of dry matter (AIDDM). At last, the AIDCP and apparent ileal digestibility of amino acids (AIDAA) in maize, barley, wheat, rapeseed meal, cottonseed meal and three mixed diets were determined with the MNBT and ileo-rectal anastomis pigs (IRAT). For the MNBT techniques, bag measuring $25{\times}40$ MM and containing 0.75 g feedstuff samples, after pre-digestion in vitro, were introduced into the ileo-rectal anastomis pigs (IRAT) gastrointestinal tract through a duodenal cannula and recovered in the ileal digesta between 6 and 12 h. later. 1. The apparent ileal digestibility of dry matter (AIDDM) and crude protein (AIDCP) of the tested samples, with the exception of fish meal, determined by MNBT were not affected by the different pepsin-HCL pretreatment times in vitro between 2.5 h. and 4 h. 2. There was no significant (p > 0.05) difference of the AIDCP and AIDDM of maize determined by the MNBT among different pepsin concentration (0.03%, 0.07% and 0.1 %) treatment in vitro. 3. The AIDCP determined with the MNBT was affected by the washed and unwashed recovered bags from the ileal digesta. 4. The AIDCP and AID amino acids (AIDAA) of maize, barley, wheat, rapeseed meal, soya-bean meal, cottonseed meal and three mixed diets from the MNBT, with a solution of 0.01N HCL (PH 2) and 0.1% of pepsin concentration, a pepsin-HCL pretreatment time in vitro or 4h. and a washing time of the recovered bag from the ileal digesta compared well with those from the IRAT. The linear regression analysis showed a significant correlation (p < 0.01) of AIDCP and AIDDA between the IRAT and MNBT.

• 한국축산시설환경학회지
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• 제11권2호
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• pp.125-134
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• 2005

본 연구는 남은 음식물에 수분흡수제 또는 영양 보충제로 유기성 부산물인 제과부산물, 밀기울, 맥강, 육계분 등을 혼합한 후 rotary drum 방식의 반건식 살균처리 및 호기적 숙성 과정 별 혼합사료 내의 미생물 분포 및 사료단백질 pepsin 소화율과 in vitro 영양소 소화율을 측정하여 실제 동물 급여 시의 효능을 사전 예측하고자 실시하였다. 살균 처리 공정($80^{\circ}C$에서 30분간)은 전반적으로 균수를 감소시키는 경향과 곰팡이를 사멸시키는 효과가 있었으며(P<0.05), 연이은 숙성 과정은 살균과정에서 감소된 lactic acid bacteria를 증식시키는 효과가 있었다(P<0.05). 열처리 살균 공정은 혼합사료 단백질의 pepsin 소화율을 감소시켰다(P<0.05). 사료원료 건물 및 유기물의 in vitro 소화율은 제과부산물>밀기울>남은 음식물=맥강 순으로 높았다. 상기한 연구 결과는 반건식 남은 음식물 혼합사료의 동물 급여시 중요한 기초 자료로 활용될 것이다.

• Journal of Nutrition and Health
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• 제28권10호
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• pp.986-994
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• 1995

This study was to examine the effect of phytate on the protein digestibility and calcium, iron and zinc availability in phytase treated soymilks digested with pepsin and pepsin-pancreatin in vitro. Also, the bending between phytate and protein in soymilks was investigated by means of SDS-PAGE. The content of phytate in soymilk was reduced by phytase treatment. As the content of phytate decreased, the protein digestibility increased in soymilk treated with the digest enzymes in vitro. The reduction of phytate content in soymilk improved the availability of all calcium, iron and zinc. Although the availability of calcium increased, the amount of change was small. The phytate reduction increased most the availability of iron. A number of bands of high molecular weight protein in soymilk disappleared in SDS-PAGE by lowering the phytate content with phytase treatement on soymilk.

• Journal of Nutrition and Health
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• 제10권2호
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• pp.27-34
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• 1977

The present study was carried out to evaluate the nutritional quality of rabbit meat protein. The composition of amino acids contained in rabbit meat was compared with those of other animal meats such as beef, pork and chicken. Also included in this study was the question whether the cooking and storage conditions affect the amino acid composition and the pepsindigestibility of rabbit meat protein. The results are summarized as follows: 1. The large variation observed from sample to sample of EAA (essential amino acid) composition in rabbit meat was found to be an interesting but peculiar property of rabbit meat protein. The most limiting amino acid of rabbit meat protein was phenylalanine, whereas methionine was the first limiting amino acid of both beef and pork proteins. Chemical scores of various meat proteins were 68, 65, 66, and 74 for rabbit meat, beef, pork, and chicken respectively. 2. In pan roasting, the EAA damaged most by heat was methionine (15%). When cooked after two months of frozen storage, lysine decreased most. 3. Higher pepsin digestibility was obtained by cooking rabbit meat after seasoned in alcohol, ginger juice, and other spices compared with various other cooking conditions without seasoning. The pepsin digestibility value was even higher for the seasoned meat than for the raw meat. 4. Among various meats tested the rabbit meat showed the lowest pepsin digestibility. 5. A simple measurement of released methionine could be used to determine relative digestibility instead of measuring $NH_2-N$ content after pepsin digestion. From all the results obtained in this study it can be concluded that rabbit meat is a good Protein food item when used fresh and stored properly to prevent rancidity problems. It is suggested to study further the peroxidation effect of unsaturated fatty acids on protein quality. This study was supported by the Ministry of Science and Technology in Korea.

• Asian-Australasian Journal of Animal Sciences
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• 제17권3호
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• pp.379-385
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• 2004

Two trials in vivo and in vitro were conducted, in vivo to determine the apparent digestibility of gross energy, crude protein, dry matter, acid detergent fiber, neutral detergent fiber and apparent digestible energy in 10 corn by-products. In vivo the diets included one basal corn diet, four corn gluten meal diets, four corn distillers dried grains with solubles diets and two corn distillers dried grains diets using the different methods, 12 crossbred barrows weigh $40{\pm}$1.6 kg were allocated into individual metabolic crate, according to a $6{\times}6$ Latin square design. In vitro using flask technique, filter bag technique and dialysis tubing technique, the digestibilities of gross energy, crude protein and dry matter in corn gluten meal and corn distillers dried grains with solubles were investigated. Pepsin, pancreatin, intestinal fluid, rumen fluid and cellulase were used in incubation. The results showed that correlation coefficient was 0.73 in corn distillers dried grains with solubles between the digestibility of crude protein and acid detergent fiber in vivo (p<0.01); and correlation coefficient was 0.68 in corn distillers dried grains with solubles between the digestibility of gross energy and neutral detergent fiber in vivo (p<0.01). Apparent digestible energy (DE) of corn by-products in pig total tract was predicted by the percentage of crude protein (CP) and the content of gross energy (GE) in feedstuff. The equation: DE=5,601.09+26.69$\times$CP %-0.5904$\times$GE, ($R^2=0.72$). In vitro, filter bag technique was more convenient; furthermore, the digestibility for the treatments (pepsin+pancreatin+rumen fluid and pepsin+pancreatin+cellulase) was better.

• 한국식품과학회지
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• 제26권5호
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• pp.603-608
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• 1994

대두식품의 가공 및 발효에 따른 phytate 함량의 변화, 그 함량이 pepsin과 pancreatin에 의한 단백질 소화에 미치는 영향, 그리고 전기영동법에 의한 단백질 분획의 변화를 조사하였다. 황색콩의 phytate 함량은 2.4%이었고 가공 및 발효에 따라 감소하여 두유 0.2%, 두부 0.7%, 비지 0.4%로, 청국장 0.9%, 메주 1.4%, 간장 0.2%, 된장 1.0%로 나타났다. Phytate/protein 비율은 pepsin에 의한 단백질 소화율과 상관성이 없었으나 pancreatin에 의한 단백질 소화율과는 부(負)의 상관관계를 나타냈다(p<0.01, r= -0.73). 전기영동 결과 콩을 물에 불리면 수용성 분획이 변화되었고 두유 제조시 비지에는 주로 저분자량 밴드들이 새로이 나타났다. 발효 과정에서는 단백질이 분해되어 밴드가 거의 나타나지 않았다.

• 한국토양비료학회지
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• 제38권5호
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• pp.253-258
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• 2005

토양에서 작물에게 공급되는 질소를 알아내는 것은 토양과 환경을 보전하면서 농사를 지속적으로 영위하기 위하여 필수적이다. 토양의 질소 공급력은 질소 무기화 포텐셜 등과 같이 직접 측정을 하거나 토양 유기물 등을 이용하여 간접적인 추정으로 정하고 있다. 토양의 질소 공급력을 직접 측정하는 방법이 가장 좋은데 질소 무기화 포텐셜은 장기간에 걸쳐 실험을 해야 하기 때문에 실용적으로 사용하기 어렵고 토양중 무기태 질소는 존재하는 양이 적으면서도 행동이 복잡하여 질소공급력지표로 부적합하여 토양유기물을 이용하여 간접적으로 추정하는 방법이 많이 사용되고 있다. 본 연구는 단기적인 분석방법을 통하여 질소공급력을 알아낼 수 있는 방법을 찾기 위하여 우리나라 논과 밭 6점에 대하여 질소무기화포텐셜을 측정하고, 질소무기화포텐셜과 가장 관계가 갚은 pepsin분해 방법 조건을 설정하고, 이 방법을 토양이 최고 수량을 얻기 위하여 필요한 질소의 양과의 관계를 토양유기물함량과의 비교를 통하여 검증하였다. 논과 밭 토양 6점에 대하여 질소 무기화량을 정량한 결과 토양에서 공급하는 질소의 범위인 $63.1-156.2mg\;N\;kg^{-1}$으로 나타났다. Pepsin을 이용하여 분석하는 방법은 토양 5 g을 0.02% pepsin으로 $30^{\circ}C$에서 30분간 항온하고, 분해된 질소의 침출은 흔합 후의 농도가 2 M KCl이 되도록 하여 30분간 침출하여 아미노태 질소를 정량하는 방법으로 설정하였는데 여러 가지 무기태질소 분석방법보다 질소무기화포텐셜과 가장 높은 상관관계를 나타내었다. 이 방법은 최고수량을 얻기 위하여 필요한 질소량과의 관계에서 토양의 질소공급력 지표로 사용하고 있는 토양유기물보다 더 높은 상관관계를 나타내었다. 따라서 토양의 질소공급력을 정량할 수 있는 방법으로 pepsin을 이용한 토양질소 분해 방법을 이용할 수 있는 것으로 판단되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제15권10호
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• pp.1507-1516
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• 2002

Meat and bone meal is a valuable protein and mineral source in diets of production animals and contributes to the protein, energy and mineral component of diets. The aim of the present study was to more accurately characterise the apparent ileal amino acid digestibility of meat and bone meals produced in New Zealand and evaluate routine in vitro assays used in practise to measure meat and bone meal quality. A total of 94 commercial meat and bone meals from 25 New Zealand rendering plants over a two and a half year period were analysed for proximates, gross energy, gross amino acid content (incl. hydroxyproline, hydroxylysine and lanthionine), apparent ileal amino acid digestibility, pepsin nitrogen digestibility, protein solubility and bone content. The mean crude protein content of the 94 meat and bone meal samples was 56.8% with a range of >35% units and a coefficient of variation of 9.8%. The mean crude fat and ash content were 10.0 and 28.4% respectively. These latter components showed a large range (16 and 43%, respectively) with coefficients of variation above 22%. Amino acid digestibility between samples was highly variable with lysine and sulphur amino acids digestibility ranging between 45.8-89.0 and 38.2-85.5%, respectively. Pearson correlation coefficients are presented between crude protein content and individual gross amino acids, crude protein content and individual digestible amino acid content, and pepsin N digestibility and individual digestible amino acid content. There was a significant relationship between the digestible amino acid nitrogen content and the crude protein content while pepsin nitrogen digestibility was not correlated to ileal amino acid nitrogen digestibility (r=-0.06). Meat meals with a high protein content had relatively low hydroxyproline and hydroxylysine levels something that was attributed to the levels of collagen from bone. The data indicated that lanthionine (formed upon heat treatment of cysteine with a hydroprotein) is not a good indicator of the heat treatment employed to meat and bone meals. Step-wise multiple regression equations to predict the apparent digestible content of amino acids from rapid in vitro assays are presented. The most selected variables included ash and crude fat content. In general the equations derived for the essential amino acids had a higher degrees of fit (R2) compared to the non-essential amino acids. The R2 for the essential amino acids ranged from 0.43 for histidine and 0.68 for leucine. These equations provide a means of more rapidly estimating the apparent ileal digestible amino acid content (protein quality) of meat and bone meal using standard analyses.

• 한국가금학회지
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• 제11권1호
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• pp.13-17
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• 1984

동물성 단백질사료인 가수분해 우피분을 16시간 pepsin-HC$\ell$용액 농도별 (0.2%, 0.1%, 0.05%) 0.025%, 0.0125%과 0.2% pepsin-HC$\ell$ 소화시간별 (4, 8, 12, 16, 20시간)로 45$^{\circ}C$ incubator에서 소화시키고 0.75 N HC$\ell$로 acid blank value를 보정하여 in vitro pepsin-HC$\ell$ 단백질 소화율을 측정하였는데 그 결과는 다음과 같다. 1. 0.2% pepsin-HC$\ell$에서 4, 8, 12, 16, 20시간 소화시 단백질 소화율은 각각 66.31 %, 80.69%, 83.27%, 84.65%, 87.45%로서 80% 이상의 소화시간은 8시간이었고 시간이 증가하면 소화율도 향상되었다. 2. Pepsin-HC$\ell$농도 0.2%, 0.1%, 0.05%, 0.025%, 0.0125% 수준에서 16시간 소화시 단백질 소화율은 각각 85.10%, 82.08%, 76.18%, 74.67%, 64.82%로 pepsin-HC$\ell$농도가 낮아질수록 단백질 소화율도 낮아졌다.

• 한국식품과학회지
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• 제6권2호
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• pp.70-74
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• 1974

한우(韓牛)의 round muscle과 오징어 근육(筋肉)을 여러 가지 조리조건(調理條件)으로 처리(處理)하여 pepsin에 의한 in vitro 소화율(消化率)과 소화과정중(消化過程中)의 amino태(態) N, 유기용매(有機溶媒) 처리(處理)가 소화율(消化率)에 미치는 영향(影響)을 AOAC에 의한 semi-micro Kjeldahl법(法)으로 실험(實驗)한 결과(結果)는 다음과 같다. 1. 소화율(消化率)은 우육(牛肉)에서 autoclaving, frying, raw, freezing, roasting, boiling, dry heating의 순서이며 오징어육(肉)에서는 raw, autoclaving, boiling, freezing, frying, dry heating, roasting의 순서로 높았다. 2. Amino태(態) N는 우육(牛肉), 오징어육(肉) 공(共)히 소화율(消化率)이 높은 순서로 비례해서 증가(增加)한다. 3. 우육(牛肉)과 오징어육(肉)의 raw condition에서 ether처리(處理)가 소화율(消化率)에 거의 영향을 미치지 않는다.