• Title/Summary/Keyword: PepA

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Identification of a mimotope of an infectious bronchitis virus S1 protein

  • Zhou, Jingming;Li, Jianan;Li, Yanghui;Liu, Hongliang;Qi, Yanhua;Wang, Aiping
    • Journal of Veterinary Science
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    • v.22 no.4
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    • pp.49.1-49.6
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    • 2021
  • The S1 protein of the infectious bronchitis virus (IBV) is a major structural protein that induces the production of the virus-neutralization antibodies. The monoclonal antibody against the IBV M41 S1 protein was used as a target for biopanning. After three rounds of biopanning, randomly selected phages bound to the monoclonal antibody. Sequence analysis showed that the dominant sequence was SFYDFEMQGFFI. Indirect competitive enzyme-linked immunosorbent assay showed that SFYDFEMQGFFI is a mimotope of the S1 protein that was predicted by PepSurf. The mimotope may provide information for further structural and functional analyses of the S1 protein.

Changes in Carotene Content of Chinese Cabbage Kimchi Containing Various Submaterials and Lactic Acid Bacteria during Fermentation (배추김치의 숙성중 부재료와 젖산균에 따른 Carotene 의 함량변화)

  • 장경숙;김미정;오영애;강명수;김순동
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.1
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    • pp.5-12
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    • 1991
  • the Chinese cabbage kimchi was fermented with the various submaterials such as hot pep-per garlic ginger leek green onion fermented anchovy juice and sugar according to the average contents of each submaterial described in the 39 kinds of references. And then the effects of each submaterial and lactic acid bacteria such as L. brevis. Leu. mesenteroides. P cerevisiae and L. plantarum on the content of carotenes were investigated, The major carotene in kimchi was $\beta$-carotene. And also $\delta$-carotene and $\alpha$-carotene were detected. Contents of $\beta$-carotene and total carotene were high in the kimchi containing leek red pepper powder green onion and fermented anchovy juice as a submaterial. But the kimchi containing or omitting the other submaterials were litter affected to the contents of carotene. Contents of $\beta$-carotene and total carotene were high in kimchi fermented with Leu. msenteroides L. brevis and P. cerevi-siae as a starter but was low with L plasntarum.

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Modulation of Phosphoenolpyruvate Metabolism of Anaerobiospirillum succiniciproducens ATCC 29305

  • Yoo, Jin Young;J. Gregory Zeikus
    • Journal of Microbiology and Biotechnology
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    • v.6 no.1
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    • pp.43-49
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    • 1996
  • Modulation of the catabolic PEP-pathway of Anaerobiospirillum succiniciproducens was tried using some enzymatic inhibitors such as gases and chemicals in order to enhance succinic acid production. 10$\%$ CO increased the succinic acid/acetic acid (S/A) ratio but inhibited growth as well as production of succinic and acetic acid. Hydrogen gas also increased the S/A ratio and inhibited the synthesis of pyruvate: ferredoxin oxidoreductase when used in mixture with $CO_2$, Catabolic repression by acetic, lactic and formic acid was not recognized and other modulators such as glyoxylate, pyruvate derivatives, arsenic salt, phosphate and sulfate were shown not to be effective. Magesium carbonate was shown effective for repressing acetate production. Palmitic acid, myristic acid and phenylalanine did not affect acetate production but carprylic acid completely inhibited growth.

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Effect of Core Sand on the Properties of System Sands Using Domestic Active Bentonite (국산 벤토나이트를 사용한 시스템사의 성질에 미치는 증자사의 영향에 관한 연구)

  • Cheon, Byoung-Wook;Choi, Chang-Ock
    • Journal of Korea Foundry Society
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    • v.9 no.5
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    • pp.403-412
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    • 1989
  • This study has been carried out to investigate into the properties of molding sands containing various core sands, $CO_2$, Shell, Furan, Pep Set and Cold Box, in the system sands using domestic active bentonite. The properties of system sands with 5% bentonite and 3% moistures containing baked core sands and no-baked core sands were varied by the ratio of core sands. The system sand containing no-baked core sands had been the poor bonding force and rough grain surface than those of the baked core sands. The L. O. I of system sand containing 30% organic binders core sands were more than inorganic binders core sands. It has been found that the no-baked core sands were necessary have to reclaim for using molding sand.

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Purification and characterization of antifungal compounds produced by Bacillus subtilis KS1 (Bacillus subtilis KS1이 생산하는 항진균물질의 정제 및 특성)

  • Ryoo, Sung-Woo;Maeng, Hack-Young;Maeng, Pil-Jae
    • The Korean Journal of Mycology
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    • v.24 no.4 s.79
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    • pp.293-304
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    • 1996
  • A bacterial strain, KSl, possessing strong antifungal activity was isolated from soil samples of ginseng fields and identified as Bacillus subtilis. In greenhouse test, the culture filtrate of B. subtilis KS1 showed strong protective effect against several fungal diseases of agricultural plants such as cucumber gray mold and wheat leaf rust. In addition, the crude butanol fraction of the culture filtrate exhibited antagonistic effect against several fungi including plant or human pathogens, such as Botrytis maydis, Chytridium lagenarium and Candida albicans. The antifungal compound, SW1, produced by B. subtilis KS1 was purified through consecutive chromatographic separations on a pep-RPC column and a ${\mu}$ Bondapak $C_{18}$ reverse phase column. Temperature and pH showed little effect on the stability of the compound in the ranges $-20-121^{\circ}C$ and pH 4.0-10.0, respectively. The composition and structural characteristics of SW1 were analysed by HPLC and by $^1H-,\;^1H-^1H-COSY$, NOESY, COSY-NOESY and HOHAHA NMR spectroscopy, respectively, which revealed that the compound belongs to iturin A, a typical cyclic antifungal compound produced by B. subtilis. In contrast to the previously reported iturin A compounds which have one or no $-CH_3$ side chain in the hydrophobic hydrocarbon chain of ${\beta}-amino$ acids, SW1 was shown to have a ${\beta}-amino$ acid containing 12-carbon skeleton with two $-CH_3$ side chains.

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Distributed Space-Time Coded Non-Orthogonal DF Protocols with Source Antenna Switching

  • Jin, Xianglan;Yang, Jae-Dong;No, Jong-Seon;Shin, Dong-Joon
    • Journal of Communications and Networks
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    • v.12 no.5
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    • pp.492-498
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    • 2010
  • In this paper, a new distributed space-time coded (DS-TCed) non-orthogonal decode-and-forward (NDF) protocol with source antenna switching (SAS) is proposed, where two antennas associated with each radio frequency chain can be alternatively used in the first and second phases. Several DSTC schemes for the NDF with SAS (NDF-SAS) protocol are proposed and their average pairwise error probability for the error-free source-relay (SR) channel is also derived. The simulation results show that the NDF-SAS protocol achieves larger diversity order than the NDF protocol under the error-free and erroneous SR channels.

The composition control of ITO/PET by plasma emission monitor (PEM을 이용한 ITO/PET film의 조성 제어)

  • 한세진;김용환;김영환;이택동
    • Journal of the Korean Vacuum Society
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    • v.8 no.4A
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    • pp.438-444
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    • 1999
  • The characterization of the reactively sputtered ITO layer on the PET film has been studied. The PEM device has been used to determine the optimum stoichimetry through control of the amount of oxygen incorporated into the alloy target and the optimum operation conditions to produce films with the highest electrical conductivity and visible transparency. The PEP film was pre-treated under the plasma discharge condition to remove the adsorbed gases and to modify the surface morphology. The results revealed that by adjusting the flow rate of oxygen with the spectral intensity of indium target, the composition of plasma gas can be kept constant during the entire deposition period. The resistivity of ITO film obtained was fond to be about 37$\Omega\Box$, and the transmittance of visual range was about 86%.

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A Study On Policy Based Network Using COPS Protocol (COPS 프로토콜을 이용한 정책 기반 네트워크 연구)

  • Kim, Young-Ha;Yuk, Dong-Cheol;Park, Seung-Seob
    • Proceedings of the Korea Information Processing Society Conference
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    • 2003.11b
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    • pp.1149-1152
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    • 2003
  • 인터넷의 폭발적인 성장과 함께 미래의 네트저크에서 필수적으로 지원되어야 할 것이 QoS이다. 그러나 인터넷 환경은 현재 새롭게 등장하는 실시간, 멀티미디어, 그리고 멀티캐스팅 서비스를 제공하기 위한 응용 프로그램들을 지원하기에는 적당하지 않다. 또한 양질의 서비스를 받고자 하는 사용자들의 요구 역시 만족시키기 어렵다. 그러므로, 원하는 서비스의 질을 제공하기 위해서는 무엇보다도 네트워크 기반 구조가 이를 제공하여야 한다. 본 논문은 Linux 기반 환경에서의 정책을 적용할 수 있는 COPS의 구현과 정책의 서버에 해당하는 PDP와 클라이언트인 PEP 구현, 서버와 사용자간의 인터페이스 구현에 초점을 두었다.

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A Protocol Conversion Mechanism between Terrestrial and Satellite Communication Links (지상망과 위성망간의 특성 차이를 고려한 프로토콜 변환 방식에 관한 연구)

  • 이병일;원유헌;송하윤;서학금
    • Proceedings of the Korean Information Science Society Conference
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    • 2004.10c
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    • pp.553-555
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    • 2004
  • 본 연구는 정지궤도 위성 환경에서 지상망과 위성망 간의 특성 차이로 인해 필수적으로 행해져야 하는 프로토콜의 변환에 대한 연구이다. 정지궤도 위성 환경은 높은 에러율과 큰 지연시간을 가진다. 또한 기존 지상망과 위성망 간의 높은 전송 지연 차이에 따른 버퍼 혼잡으로 인한 데이터의 손실을 피할 수 없다. 따라서 본 연구에서는 상이한 링크가 혼재된 데이터 네트워크에서 각 링크를 특성에 따라 분할하여 구간별로 독립적인 데이터 전송 및 재전송을 수행하도록 하며 전체적인 데이터 전송 처리량을 향상시키는 Spoofing 기술 및 기존 TCP 프로토콜을 대신하여 위성 프로토콜인 STP(Satellite Transport Protocol)을 적용한 PEP (Performance Enhancing Proxy) 분할 연결을 연구하였다.

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Chemical Modification of Yeast Farnesyl Protein Transferase Expressed in E. coli

  • Kim, Hyun-Kyung;Yang, Chul-Hak
    • Bulletin of the Korean Chemical Society
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    • v.27 no.4
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    • pp.529-534
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    • 2006
  • Chemical modification of the S. cerevisiae farnesyl protein transferase (FPT) with CMC, phenylglyoxal and DEPC resulted in enzyme inactivation, depending upon the reagent concentration. The peptide substrate GST-PEP-I, a GST-fused undecapeptide mimicking the C-terminus of $p21^{Ki-ras}$, protected the enzyme against inactivation by CMC which is specific to either aspartate or glutamate, while the other substrate farnesyl pyrophosphate (FPP) showed protection against phenylglyoxal which is the specific modifier of arginine residues, dependent on the substrate concentrations. Neither of the two substrates protected the enzyme against histidine inactivation by DEPC. It is suggested that there is at least one aspartate or glutamate residue at the peptide substrate binding site, and that at least one arginine residue is located at the binding site of FPP. There also seems to be at least one histidine residue which is critical for enzymic activity and is exposed toward the bulk solution, excluded from the substrate binding sites.