Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Identification of a mimotope of an infectious bronchitis virus S1 protein

  • Received : 2020.07.16
  • Accepted : 2020.10.07
  • Published : 2021.07.31
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Abstract

The S1 protein of the infectious bronchitis virus (IBV) is a major structural protein that induces the production of the virus-neutralization antibodies. The monoclonal antibody against the IBV M41 S1 protein was used as a target for biopanning. After three rounds of biopanning, randomly selected phages bound to the monoclonal antibody. Sequence analysis showed that the dominant sequence was SFYDFEMQGFFI. Indirect competitive enzyme-linked immunosorbent assay showed that SFYDFEMQGFFI is a mimotope of the S1 protein that was predicted by PepSurf. The mimotope may provide information for further structural and functional analyses of the S1 protein.

Keywords

Acknowledgement

This research was supported by funds from the National Key Research and Development Program of China (2016YFD0500800).

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