• Archives of Pharmacal Research
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• 제26권12호
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• pp.1024-1028
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• 2003

A prolyl endopeptidase inhibitor was isolated from the ethyl acetate soluble fraction of Phyllanthus ussurensis. The active compound was identified as an ellagitannin, corilagin. It was shown to non-competitively inhibit prolyl endopeptidase (PEP) with the $IC_{50}$ value of $1.17 \times $10^{-6}\mu$M. The Ki value was $6.70 \times 10^{-7}$ M. Corilagin was less inhibitory to other serine proteases such as chymotrypsin, trypsin, and elastase, indicating that it was relatively a specific inhibitor of PEP. Corilagin also effectively inhibited reactive oxygen species such as hydroxide and superoxide anion radical, hydrogen peroxide, and DPPH. Especially, corilagin showed potent scavel1ging activity on the superoxide anion radical in the ESR method ($IC_{50} =3.79 \times 10^{-6}$M) as well as xanthine oxidase system.

• 약학회지
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• 제41권2호
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• pp.153-160
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• 1997

One hundred and seventy crude drugs were screened for prolyl endopeptidase (PEP) inhibitors. Among them, 80% methanolic extract of 18 medicinal plants such as Polygonum cuspi data, Sanguisorba officinalis, Eugenia caryophyllata, Rubus coreanus, Cinnamomum cassia (Cassiae Cortex and Cinammomi Ramulus), Rheum palmatum, Ulmus pumila, Sorbus commixta, Areca catechu, Uncaria sinensis, Terminalia chebula, Caesalpinia sappan, Nelumbo nucifera, Machilus thunbergii, Paeonia moutan, Elscholtzia patrini and Cynomorium coccineum inhibited more than 70% of PEP activity at a concentration of 40 ppm. The active principles of P. moutan, M. thunbergii, T chebula, A. catechu, S. commixta, R. palmatum, R. coreanus, E. caryophyllata and P. cuspidata were transferred into organic solvents, which showed more than 75% inhibition at 5 ppm.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제10권10호
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• pp.4957-4976
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• 2016

This paper investigates the error performance of the amplify-and-forward (AF) relaying systems in the context of full-duplex (FD) communication. In addition to the inherent self-interference (SI) due to simultaneous transmission and reception, coexistent FD terminals may cause crosstalk. In this paper, we utilize the information exchange via the crosstalk channel to construct a particular distributed space-time code (DSTC). The residual SI is also considered. Closed-form pairwise error probability (PEP) is first derived. Then we obtain the upper bound of PEP in high transmit power region to provide more insights of diversity and coding gain. The proposed DSTC scheme can attain full cooperative diversity if the variance of SI is not a function of the transmit power. The coding gain can be improved by lengthening the frame and proper power control. Feasibility and efficiency of the proposed DSTC are verified in numerical simulations.

• 산업융합연구
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• 제21권7호
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• pp.93-104
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• 2023

본 연구는 농촌에 거주하는 만성 조현병 대상자에게 조현병 환자를 위한 능력강화프로그램(PEPS)을 제공하고 능력강화, 내재화된 낙인, 자존감 및 삶의 질에 미치는 효과를 파악하기 위한 비동등성 대조군 반복측정설계를 이용한 유사실험연구이다. 연구 대상자는 O지역의 정신건강복지센터에 등록된 만성 조현병 대상자 22명(실험군: 10명, 대조군 12명)이다. 실험군에게는 8주동안 주 2회, 총 16회기로 구성된 PEPS를 제공하였다. SPSS/WIN 26.0을 이용하여 χ²-test, independent t-test, Fisher's exact test 및 repeated measures ANOVA로 수집된 자료를 분석하였다. 연구 결과 실험군은 자존감(F=6.09, p=.005) 및 삶의 질(F=3.37, p=.044)이 대조군에 비해 개선된 것으로 나타났지만, 능력강화와 내재화된 낙인에 유의한 차이가 없었다. 이러한 연구 결과는 PEPS가 대상자의 자존감과 삶의 질을 향상하는데 효과적인 개입이 될 수 있음을 나타낸다.

• The Journal of Korean Physical Therapy
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• 제16권1호
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• pp.1-13
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• 2004

Airway clearance technique are used to aid in mucus clearance in a variety of disease states. In this review I discuss airway physiology including airway mucus, action of airways, and airway resistance and review the literature and theory regarding forced expiratory technique, active cycle of breathing technique, and autogenic drainage. Also, I look at the appropriate device such as positive expiratory pressure mask(PEP mask), Flutter, and HFCWO(Vest system) which can be applied in the field of respiratory physical therapy. This study is provided as the basic resource regarding the application method of respiratory physical therapy.

• 천문학논총
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• 제27권4호
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• pp.311-316
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• 2012

Lyman break Galaxies are galaxies selected in the rest-frame ultraviolet. But, one important and missing information for these Lyman break galaxies is the amount of dust attenuation. This is crucial to estimate the total star formation rate of this class of objects and, ultimately, the cosmic star formation density. AKARI, Spitzer and Herschel are therefore the major facilities that could provide us with this information. As part of the Herschel Multi-tiered Extragalactic Survey, we have began investigating the rest-frame far-infrared properties of a sample of more than 4,800 Lyman Break Galaxies in the GOODS-North fiels. Most LBGs are not detected individually, but we do detect a sub-sample of 12 objects at 0.7 < z <1.6 and one object at z = 2.0. The ones detected by Herschel SPIRE have redder observed NUV-U and U-R colors than the others, while the undetected ones have colors consistent with average LBGs at z > 2.5. We have analysed their UV-to-FIR spectral energy distributions using the code cigale to estimate their physical parameters. We find that LBGs detected by SPIRE are high mass, luminous infrared galaxies. They also appear to be located in a triangle-shaped region in the $A_{FUV}$ vs. $logL_{FUV}$ diagram limited by $A_{FUV}$ = 0 at the bottom and by a diagonal following the temporal evolution of the most massive galaxies from the bottom-right to the top-left of the diagram. In a second step, we move to the larger COSMOS field where we have been able to detect 80 Lyman break galaxies (out of ~ 15,600) in the far infrared. They form the largest sample of Lyman break galaxies at z > 2.5 detected in the far-infrared. We tentatively name them Submillimeter Lyman break galaxies (S-LBGs).

• Journal of Plant Biotechnology
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• 제49권3호
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• pp.250-256
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• 2022

This study was conducted to measure changes in polyphenol components and antioxidant effects of Cirsium Lineare (Thunb.) after fermentation by lactic acid bacteria. First, Cirsium Lineare (Thunb.) extract (CE, unfermented) and Cirsium Lineare (Thunb.) extract fermented with Lactobacillus paracasei (FCE) were prepared. Changes in components resulting from fermentation were confirmed through changes in polyphenol compound content and silymarin derivative pattern, and antioxidant activity was confirmed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, and ferric reducing antioxidant power (FRAP) analyses. As a result, polyphenol contents of CE and FCE were confirmed as 21.94 ± 1.15 and 67.90 ± 4.48 mg GAE/g, respectively. Both values were increased approximately three times by fermentation, and there was also a change in the silymarin derivative pattern. In the case of DPPH radical RC₅₀ values in particular, CE and FCE were confirmed to inhibit DPPH radicals by 50% at concentrations of 129.44 ± 5.85 and 50.00 ± 3.47 ㎍/mL, respectively, with the FCE value approximately 2.5 times lower than that of CE. In addition, ABTS radical scavenging and FRAP activity were confirmed to share similar trends as DPPH radical scavenging activity. When CE and FCE were compared, FCE showed a better antioxidant effect overall. In conclusion, this study suggested that FCE prepared through lactic acid bacteria fermentation may be utilized as a powerful antioxidant material.

• 한국해양바이오학회지
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• 제14권1호
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• pp.9-24
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• 2022

Low-molecular weight peptides derived from fish collagen exhibit several bioactivities, including antioxidant, antiwrinkle, antimicrobial, antidiabetic, and antihypertension effects. These peptides are also involved in triglyceride suppression and memory improvement. This study aimed to investigate the optimal processing condition for preparing low-molecular weight peptides from flounder skin, and the properties of the hydrolysate. The optimal processing conditions for peptic hydrolysis were as follows: a ratio of pepsin to dried skin powder of 2% (w/w), pH of 2.0, and a temperature of 50℃. Peptic hydrolysate contains several low-molecular weight peptides below 300 Da. Gly-Pro-Hyp(GPHyp) peptide, a process control index, was detected only in peptic hydrolysate on matrix-assisted laser desorption/ionization-time-of-flight(MALDI-TOF) spectrum. 2,2'-azinobis-(3-3-ethylbenzothiazolline-6- sulfonic acid(ABTS) radical scavenging activity of the peptic hydrolysate was comparable to that of 1 mM ascorbic acid, which was used as a positive control at pH 5.5, whereas collagenase inhibition was five times higher with the peptic hydrolysate than with 1 mM ascorbic acid at pH 7.5. However, the tyrosinase inhibition ability of the peptic hydrolysate was lower than that of arbutin, which was used as a positive control. The antibacterial effect of the peptic hydrolysate against Propionibacterium acne was not observed. These results suggest that the peptic hydrolysate derived from a flounder skin is a promising antiwrinkle agent that can be used in various food and cosmetic products to prevent wrinkles caused by ultraviolet radiations.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1997년도 춘계학술대회
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• pp.119-119
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• 1997

Valacyclovir is a L-valyl ester prodrug of acyclovir which is a highly effective and selective antiviral agent in the treatment of herpes virus diseases. Valacyclovir is rapidly and almost completely converted to acyclovir and increases the oral bioavailability of acyclovir three to five fold. However, the intestinal absorption mechanism of valacyclovir is not clear. If the improved absorption mechanism of valacyclovir is fully understood, it will provide a rationale of designing the amino acid ester prodrugs of polar drugs containing hydroxyl group. The main objective of our present study is to characterize the membrane transport mechanism of valacyclovir. Methods : Intestinal absorption of valacyclovir was investigated by using in-situ rat perfusion study and its wall permeability was estimated by modified boundary layer model. The membrane transport mechanism was also investigated through the uptake study in Caco-2 cells and in CHO-hPepTl cells. Results : In the rat perfusion study, the wall permeability of valacyclovir was ten times higher than acyclovir and showed concentration dependency, Valacyclovir also demonstrated a D,L stereo-selectivity with L-isomer having an approximately five-fold higher permeability than D-isomer. Mixed dipeptides and cephalexin, which are transported by dipeptide carriers, strongly competed with valacyclovir for the intestinal absorption, while L-valine did not show any competition with valacyclovir. This indicated that the intestinal absorption of valacyclovir could be dipeptide carrier-mediated. In addition, the competitive uptake study in Caco-2 cells presented that dipeptides reduced the valacyclovir uptake but valine did not. Also, in IC$\sub$50/ study, valacyclovir showed strong inhibition on the $^3$H-gly-sar uptake in CHO-hPepTl cells over-expressing a human intestinal peptide transporter. Taken together, the result from our present study indicated that valacyclovir utilized the peptide transporter for the intestinal absorption.

• 원예과학기술지
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• 제35권4호
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• pp.465-479
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• 2017

Chili pepper (Capsicum annuum L.) is an economically important horticultural crop in Korea; however, various diseases, including Phytophthora root rot, anthracnose, powdery mildew, Cucumber mosaic virus (CMV), Pepper mild mottle virus (PMMoV), and Pepper mottle virus (PepMoV), severely affect their productivity and quality. Therefore, pepper varieties with resistance to multiple diseases are highly desired. In this study, we developed 20 SNP type assays for three pepper populations using Fluidigm nanofluidic dynamic arrays. A total of 4,608 data points can be produced with a 192.24 dynamic array consisting of 192 samples and 24 SNP markers. The assays were converted from previously developed sequence-tagged-site (STS) markers and included markers for resistance to Phytophthora root rot (M3-2 and M3-3), anthracnose (CcR9, CA09g12180, CA09g19170, CA12g17210, and CA12g19240), powdery mildew (Ltr4.1-40344, Ltr4.2-56301, and Ltr4.2-585119), bacterial spot (Bs2), CMV (Cmr1-2), PMMoV (L4), and PepMoV (pvr1 and pvr2-123457), as well as for capsaicinoids content (qcap3.1-40134, qcap6.1-299931, qcap6.1-589160, qdhc2.1-1335057, and qdhc2.2-43829). In addition, 11 assays were validated through a comparison with the corresponding data of the STS markers. Furthermore, we successfully applied the assays to commercial $F_1$ cultivars and to our breeding lines. These 20 SNP type assays will be very useful for developing new superior pepper varieties with resistance to multiple diseases and a higher content of capsaicinoids for increased pungency.