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A Prolyl Endopeptidase-lnhibiting Antioxidant from Phyllanthus ussurensis  

Chung, Shin-kyo (Department of Food Technology, Kyungpook National University)
Nam, Ji-Ae (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Jeon, So-Young (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Kim, Sang-ln (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Lee, Hee-Ju (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Chung, Tai-Ho (School of Medicine, Kyungpook National University)
Song, Kyung-Sik (Division of Applied Biology and Chemistry)
Publication Information
Archives of Pharmacal Research / v.26, no.12, 2003 , pp. 1024-1028 More about this Journal
Abstract
A prolyl endopeptidase inhibitor was isolated from the ethyl acetate soluble fraction of Phyllanthus ussurensis. The active compound was identified as an ellagitannin, corilagin. It was shown to non-competitively inhibit prolyl endopeptidase (PEP) with the $IC_{50}$ value of $1.17 \times $10^{-6}\mu$M. The Ki value was $6.70 \times 10^{-7}$ M. Corilagin was less inhibitory to other serine proteases such as chymotrypsin, trypsin, and elastase, indicating that it was relatively a specific inhibitor of PEP. Corilagin also effectively inhibited reactive oxygen species such as hydroxide and superoxide anion radical, hydrogen peroxide, and DPPH. Especially, corilagin showed potent scavel1ging activity on the superoxide anion radical in the ESR method ($IC_{50} =3.79 \times 10^{-6}$M) as well as xanthine oxidase system.
Keywords
Phyllanthus ussurensis; Prolyl endopeptidase; Inhibitor; Antioxidant; Corilagin; Alzheimer;
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