• Applied Biological Chemistry
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• 제22권1호
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• pp.39-41
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• 1979

우리나라 재래종(在來種)갓의 anthocyanin 색소(色素)의 구명(究明)을 위(爲)한 실험(實驗)에서 제일보(第一報)의 구조추정(構造推定)에 이어서 이들 색소(色素)의 함량(含量)을 측정(測定)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 채취시기(採取時刻)의 구별(區別)에 의한 전(全) anthocyanin의 함량(含量)은 peonidin-3-glucoside로 표시(表示)하여 봄갓이 175.5mg% 가을갓이 192.7mg%로 가을갓이 약간(若干) 더 많은 함량(含量)을 보였다. 2, 착색정도(着色程度)의 구별(區別)에 의한 전(全) anthocyanin의 함량(含量)은 peonidin-3-glucoside로 표시(表示)하여 잎의 양면착색(兩面着色)갓이 290.2mg%, 한면(面)만의 착색(着色)이 73.6mg%, 양면(兩面)모두 무착색(無着色)갓이 40.0mg%이었다. 3. 신선(新鮮)한 갓에서 peonidin-3-glucoside로 표시(表示)하여 40mg%이하의 anthocyanin 함량(含量)으로는 육안(肉眼)으로서는 전혀 갓특유(特有)의 착색(着色)을 감지(感知)할 수 없었다. 4. 갓에 존재(存在)하는 이종(二種)의 anthocyanin의 조성비(組成比)는 채취시기(採取時期)및 착색(着色)의 정도(程度)에 따라서도 거의 일치(一致)하였으며 그 조성(組成)은 peonidin-3-glucoside가 55.3% 그리고 peonidin-3-galactoside가 44.7%이었다.

• 한국미생물·생명공학회지
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• 제36권1호
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• pp.55-60
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• 2008

유색미는 회색 빛 외에 붉은색 흑은 보라색 계통의 색소를 겨 부분에 함유하고 있다. 특히, 흑미는 예로부터 건강식품으로 여겨져 왔다. 흑미(흑진주벼)는 최근에 anthocyanin포함하는 생리활성 물질원으로서 관심이 증대되고 있다. Anthocyanin은 수용성의 색소로서 flavonoid계통의 대표 색소로 알려져 왔다. 흑미에 함유되어있는 Anthocyanin으로는 cyanidin 3-O-glucoside와 peonidin 3-O-glucoside, malvidin 3-O-glucoside, pelagonidin 3-O-glucoside, delphinidin 3-O-glucoside등이 알려져 있다. 본 연구에서는, 흑미로부터 amberlite XAD7 chromatography방법을 사용해서 anthocyanin을 추출 후 HPLC와 UV-Vis spectrophotometer를 이용하여 정량분석 및 정성분석을 실시하였다. 그 결과 흑미의 anthocyanin 추출물 중 95%가 cyanidin 3-O-glucoside, 5%가 peonidin 3-O-glucoside임을 확인하였다. 그리고 in vitro상의 여러 가지 실험방법을 통하여 anthocyani의 항산화 효과를 측정하였다. 그 결과 100g/ml 농도의 anthocyanin추출물은 90%이상 linoleic acid emulsion의 과산화를 억제하였고, 55.20%의 DPPH 자유 라디칼 제거효과, 54.96%의 superoxide 자유 라디칼 제거효과, 72.67%의 과산화수소 제거효과를 보였다. 그리고 또한 높은 환원 능력을 확인할 수 있었다. 표준 항 산화 물질로는 BHA, BHT, ${\alpha}-tocopherol$을 사용하였다. 이 결과로 보아 anthocyanin추출물은 새로운 항산화 물질로서 이용될 수 있을 것이다.

• Applied Biological Chemistry
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• 제22권1호
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• pp.33-38
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• 1979

우리나라에서 채소(菜蔬)로 또 조미품(調味品)으로 많이 이용(利用)되고 있는 재래종(在來種)갓의 anthocyanin 색소(色素)의 구명(究明)을 위(爲)한 실험(實驗)에서 얻어진 결과(結果)를 요약(要約)하면 다음과 같다. 1. T.L.C.에 의(依)해 이종(二種)의 anthocyanin을 분리(分離)하였다. 2. 이들 anthocyanin의 구조구명(構造究明)을 위(爲)하여 Robinson 시험(試驗), Spectrophotometry, T.L. chromatography, 부분가수분해등(部分加水分解等)의 방법(方法)으로 각각 peonidin-3-glucoside와 peonidin-3-galctoside 임을 확인(確認)하였다.

• Natural Product Sciences
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• 제21권1호
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• pp.30-33
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• 2015

Anthocyanins are water soluble plant pigments which are responsible for the blue, red, pink, violet colors in several plant organs such as flowers, fruits, leaves and roots. In recent years, anthocyanin-rich foods have been favored as dietary supplements and health care products due to diverse biological activities of anthocyanins including antioxidant, anti-allergic, anti-diabetic, anti-microbial, anti-cancer and preventing cardiovascular disease. High-performance countercurrent chromatography (HPCCC) coupled with reversed-phase medium pressure liquid chromatography (RP MPLC) method was applied for the rapid and efficient isolation of cyanidin 3-glucoside (C3G) and peonidin 3-glucoside (P3G) from black rice (Oryza sativa L., Poaceae). The crude black rice extract (500 mg) was subjected to HPCCC using two-phase solvent system composed of tert-butyl methyl ether/n-butanol/ acetonitrile/0.01% trifluoroacetic acid (TBME/B/A/0.01% TFA, 1 : 3 : 1 : 5, v/v, flow rate - 4.5 mL/min, reversed phase mode) to give enriched anthocyanin extract (37.4 mg), and enriched anthocyanin extract was sequentially chromatographed on RP-MPLC to yield C3G (16.5 mg) and P3G (8.7 mg). The recovery rate and purity of isolated C3G were 76.0% and 98.2%, respectively, and those of P3G were 58.3% and 96.3%, respectively. The present study indicates that HPCCC coupled with RP-MPLC method is more rapid and efficient than multi-step conventional column chromatography for the separation of anthocyanins.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.47-47
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• 2021

This study was carried out to obtain a basic information for the improvement of human health and the development of variety through analysis of organic compounds, contents of three CQA(3-caffeoylquinic acid, 4-caffeoylquinic acid and 5-caffeoylquinic acid) and five anthocyanin (petunidin-3-p-cumaroylrutinoside-5-glucoside, pelargonidin-3-p-cumaroylrutin-oside-5-glucoside, peonidin-3-p-cumaroylrutinoside-5-glucoside, pelargonidin-3-p-feruloyl-rutinoside-5-glucoside and peonidin-3-feruloylrutinoside-5-glucoside)to color potatoes is Hong-young(HY) and Ja-young(JY). The analytical results on organic compounds in color potatoes were shown as follow, The contents of CQA and Anthocyanin of JY variety were shown to be higher than HY, while CQA and Anthocyanin were appeared to be highest in peel of JY. Overall, JY had higher amount of physicochemical properties than HY. The results of this study reveal the quantitative analysis of functional compounds seperated from various kind of potatoes, which will enable the acquisition of new bioactive candidates and the establishment of new profit generation models for farmers.

• 한국작물학회지
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• 제45권4호
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• pp.257-260
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• 2000

The content of anthocyanin, cyanidin 3-glucoside (C3G) and the peonidin 3-glucoside (P3G) from 591 rice cultivars and the antioxidative activities of MeOH extract from 8 rice cultivars were evaluated. Among them, C3G content of pigmented rice were ranged from 0 to 451.9mg in 100 g brown rice, while the P3G contents were in the range from 0 to 42.7mg in 100g brown rice. There was no correlation between C3G and P3G content. Total anthocyanin content ranged from 0 to 475.1mg in 100g brown rice. The antioxidative activity, the scavenging activity on DPPH (2,2-diphenyl-picryl-hydrazyl) radical of MeOH extracts from rice grain, were different according to cultivars. The activity of blockish purple pericarp rice cultivars was twice stronger than that of white pericarp cultivar. Especially, the antioxidative activity of Heugjinjubyeo was four times stronger than that of white pericarp cultivar. The scavenging effect on DPPH radical in rice extracts was related to the total anthocyanin contents of the extracts.

• 한국식품영양과학회지
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• 제29권4호
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• pp.555-560
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• 2000

자색고구마 (자미) anthocyanin 색소의 분석을 위하여 1% HCl 함유 methanol로 추출한 자색고구마 색 소액을 Amberlite IRC-50 양이온교환수지관을 통과시켜 정체하고 paper chromatography로 개별색소를 분리, 3개의 band를 얻었다. Paper chromatography에 의해 분리된 각 개별색소 에 대해 부분가수분해, aglycone의 확인, acyl group의 확인, 결합당의 확인, paper chromatography 그리고 HPLC 등을 기초로 한 각종 기기분석 결과 자색고구마의 anthocyanin 색소는 caffeic acid와 ferulic acid로 acylation된 peonidin-3-diglucoside-5-glucoside의 구조를 나타냄을 알 수 있었다.

• 농업과학연구
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• 제44권1호
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• pp.104-113
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• 2017

Anthocyanins contained in Rhododendron schlippenbachii (royal azalea) are expressed in a variety of colors and affect flower colors. R. schlippenbachii flowers of seven colors (white, red group: pink, deep pink, red, purple group: light purple, purple, deep purple) were collected from the garden around KT&G building in the college of agriculture and life science. Seven types of anthocyanins [cyanidin 3-O-diglucoside, cyanidin 3-O-arabinoside-5-O-glucoside, cyanidin 3-O-galactoside, peonidin 3-O-arabinoside-5-O-glucoside, cyanidin 3-O-glucoside, cyanidin 3-O-(6"-O-malonyl) arabinoside, cyanidin 3-O-(6"-O-coumaroyl) glucoside] turned out to be from the cyanidin and peonidin series in R. schlippenbachii flowers. Also, seven types of flavonols [azaleatin 3-O-glucoside, azaleatin 3-O-arabinoside, azaleatin 3-O-rhamnoside, quercetin 3-O-galacatoside, quercetin 3-O-glucoside, quercetin 3-O-arabinoside, quercetin 3-O-rhamnoside] were identified in R. schlippenbachii flowers. Total anthocyanin amounts decreased in R. schlippenbachii flowers in the following order: 'deep pink' (8.07) > 'red' (6.37) > 'pink' (5.35) > 'deep purple' (0.78) > 'purple' (0.43) > 'light purple' ($0.22mg{\cdot}g^{-1}$ dry weight, DW) > 'white' (not detected). Total flavonol amounts decreased in the following order: 'pink' (97.78) > 'deep pink' (63.79) > 'deep purple' (61.98) > 'white' (57.58) > 'light purple' (47.06) > 'purple' (46.76) > 'red' ($7.60mg{\cdot}g^{-1}$ dry weight, DW). This study provided the quantitative and qualitative information for the variation of anthocyanin and flavonol compounds in R. schlippenbachii flowers. Furthermore, this information can contribute to the identification of anthocyanin and flavonol compounds in other Rhododendron flowers.

• 한국식품과학회지
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• 제36권1호
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• pp.134-140
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• 2004

Anthocyanin색소의 추출은 일반적으로 산을 첨가하면 그 추출효율이 증가한다고 보고되어진 바가 있다. 따라서 본 실험에서는 campbell early 포도의 부산물인 과피로부터 항산화 물질을 추출하기 위한 최적추출조건을 확립하기 위하여 ethanol 용매에 산의 종류를 달리하여 추출한 후 anthocyanin, resveratrol, quercetin함량을 측정하고, 이들의 항산화 효과를 분석하였다. 그 결과, anthocyanin색소 중 peonidin-3-glucoside는 0.1% HCl을 첨가한 추출물에서 가장 효과적이었으며, cyanidin-3-glucoside는 오히려 산을 첨가하지 않은 ethanol 용매에서 추출한 campbell early 과피 추출물이 가장 효과적이었다. 또한 항산화 활성을 가지고 있는 페놀성 화합물인 resveratrol과 quercetin의 함량은 0.1% HCl을 첨가한 campbell early 과피 추출물에서 각각 8.1 mg/100g coats로 다른 종류의 산을 처리한 것보다 더 많은 양이 함유되어 있는 것으로 나타났다. DPPH에 의한 항산화 효과 측정에서는 산을 첨가한 추출물 모두 15분 이내에 모든 반응이 정지되었으며, 대조군에 비해 높은 전자공여능을 보였다. Lecithin을 이용한 TBARS는 citric acid와 phosphoric acid, formic acid를 첨가한 경우 각각 59.3, 60.5, 56.8%로 유의적으로 높은 수준을 보였다. 이러한 결과로부터 campbell early 과피에 0.1% HCl가 첨가된 ethanol용매로 추출시 항산화 활성이 높은 추출물을 제조할 수 있었다.

• 한국식품과학회지
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• 제10권2호
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• pp.194-202
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• 1978

In order to evaluate the utility of the anthocyanin pigments in Ganges Amaranth as an edible pigment, this study was designed to isolate and identify the anthocyanins. The anthocyanins present in leaves of Ganges Amaranth were extracted with 0.1% HCl in methanol. The extracted pigments were purified by organic solvent treatment and Amberlite CG-400 Type cation exchanger, and then separated into individual pigments by paper chromatography with n-butanol-formic acid-water(100:25:60, v/v) as a solvent system. The separated pigments were identified by their Rf values, sugar moieties, complete hydrolysis and spectral characteristics in the visible and ultraviolet regions. The amounts of individual anthocyanins were also determined. The results obtained from these experiments were as follows. 1. Chromatograms of the Ganges Amaranth extract developed with BFW yielded three anthocyanin bands. The two of the anothocyanin bands were tentatively identified as malvidin-3-glucoside(acylated with caffeic acid) in band 1 and peonidin-3-glucoside (acylated with caffeic acid) in band 2. But the anthocyanin in band 3 was not identified due to extremly low concentration. 2. The amount of total anthocyanins was 101.57 mg/100g fresh weight of leaves in which 82.15 mg of malvidin-3-glucoside (acylated with caffeic acid) and 27.20 mg of peonidin-3-glucoside(acylated with caffeic acid) were contained per 100g fresh weight. Maividin-3-glucoside acylated with the acid was, therefore, the most abundant pigment in the Ganges Amaranth.