• 한국조경학회지
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• 제31권2호
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• pp.83-93
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• 2003

This study was conducted for an understanding of a threatened plant species,“Penthorum chinense”, which is a vegetation structure and environmental characteristic in the southern part of Ibaraki, Japan. We selected distribution and contiguity area where P. chinense is not distributed and we set up 69 sites by the quardrat method. The surveyed general characters in each site include mean-height, mean-coverage, moisture condition water depth coverage and depth of litter layer. We calculated the degree of succession by the survey data and grasped the main distribution area, vegetation structure, growth environment of P. chinense by TWINSPAN classification and DCA ordination. The results of this study are as follows. First, P. chinense habitat was categorized into the riverside and abandoned paddy fields. The riverside was influenced by natural disturbance caused by flooding and drought. These conditions led to a poor P. chinense growth. The abandoned paddy fields as the artificial disturbance area have high potential value, because the area can preserve P. chinense growth space by succession control and water management. second, we executed TWINSPAN analysis based on vegetation coverage data of survey sites. The vegetation types were classified into the three categories: annual plant flora(P. chinense dominates in wet state), perennial plant flora(Solidago altissima dominates in dry state), and perennial plant flora(Phragmites communis dominates in wet state). Third, the data was ordinated by DCA. The sample sequences along the first and second DCA axes effectively reflected moisture condition, number of species, mean height, coverage of litter layer and degree of succession. Especially, P. chinense flora has the high correlation with moisture condition and number of species.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.100-100
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• 2019

This study attempted to establish a High Performance Liquid Chromatography (HPLC) analysis method for the determination of (-)-epicatechin gallate as a part of the quality control for the development of functional cosmetic materials from Penthorum chinense Pursh extracts. HPLC was performed on a Unison US-C18 column ($4.6{\times}250mm$, $5{\mu}m$) with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and methyl alcohol at a flow rate of 1.0 mL/min at $30^{\circ}C$. The analyte was detected at 280 nm. The HPLC method was performed in accordance with the International Conference on Harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation were 0.11 and 0.33 mg/mL, respectively. Calibration curves showed good linearity (r2 > 0.9999), and the precision of analysis was satisfied (less than 0.6%). Recoveries of quantified compounds ranged from 99.51 to 101.92%. This result indicates that the established HPLC method is very useful for the determination of marker compound in P. chinense Pursh extracts.

• 한국식품위생안전성학회지
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• 제30권4호
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• pp.376-382
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• 2015

Allergic diseases have rapidly increased in recent years. Mast cells play a critical role in IgE-mediated allergy responses and, therefore, closely associated with rhinitis, asthma, and atopic dermatitis. We studied anti-allergic effect of Penthorum chinense extract (PCE) in vitro and in vivo. PCE inhibited the degranulation of mast cells by antigen stimulation and its effect was dose-dependent and reversible in mast cells. PCE also suppressed the production of inflammatory cytokines such as TNF-${\alpha}$ and IL-4 by antigen in mast cells. Mechanistically, PCE inhibited the activation of Syk/LAT pathway which is a key signaling pathway for the activation of mast cells by antigen. Notably, PCE suppressed IgE-mediated allergic responses by antigen in mice. These results strongly suggest that PCE is a potential candidate for anti-allergic treatment.

• 대한화장품학회지
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• 제45권1호
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• pp.87-93
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• 2019

본 연구는 낙지다리(Penthorum chinense Pursh) 추출물을 기능성 화장품소재로 개발하기 위해 (-)-epicatechin gallate를 지표성분으로 선정하고, 품질관리를 위해 High Performance Liquid Chromatography (HPLC)를 이용하여 분석법을 개발하였다. 분석에 사용된 칼럼은 Unison $US-C_{18}$ ($4.6{\times}250mm$, $5{\mu}m$, Imtakt, USA)을 사용하여 0.05% (v/v) trifluoroacetic acid (TFA)와 메탄올을 이동상 조건으로 컬럼 온도는 $30^{\circ}C$ 에서 유속은 1.0 mL/min 로 검출파장은 280 nm에서 검출하였다. International Conference on Harmonization (ICH) 가이드라인(version 4, 2005)을 근거로 하여 특이성, 직선성, 정밀성, 정확성, 검출한계 및 정량한계를 분석하여 분석방법을 검증하였다. 검출한계 및 정량한계는 각각 0.11 mg/mL 및 0.33 mg/mL로 나타났으며, 검량곡선은 상관계수값이 0.9999로 양호한 직선성을 보였고, 정밀성 분석결과도 0.6% 이하로 확인하였다. 또한, 회수율은 99.51 ~ 101.92% 범위로 정확성이 있음을 알 수 있다. 따라서, 본 분석법은 낙지다리 추출물의 지표성분의 분석법은 적합한 시험법임이 검증되었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.34-34
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• 2020

희귀식물(LC)인 낙지다리(Penthorum chinense Pursh.)는 '수택란', '차근채'로 불리며 약용으로 사용되어 왔으나, 최근 하천개수 및 습지매립 등으로 자생지가 파괴되어 개체수가 급감하고 있다. 따라서 본 연구는 낙지다리의 종 보존 및 활용을 위하여 종자의 최적발아조건을 확인하고자 수행하였다. 종자는 2017년 국립백두대간수목원에서 수집되어 연구에 사용되기 전까지 건조 상태(15℃, 15% RH)로 시드뱅크(-20℃, 40% RH)에 저장하고 있었다. 종자의 최적발아조건을 확인하기 위하여, 항온과 변온 66조건에서 발아율과 최종발아율의 50%가 발아하는데 소요되는 일 수(T₅₀)를 조사하였으며, 생리적 휴면 타파를 위해서 GA₃(20~1000 mg·L^-1) 처리 후 발아율과 T₅₀을 조사하였다. 그리고 GA₃ 처리에 따른 유묘 생장에 대한 GA₃의 영향을 확인하기 위하여 종자 치상 30일 후 유근·아 생장에 대한 영향을 조사하였다. 낙지다리 종자는 항온보다 변온에서 발아율 증가와 T₅₀ 감소를 보였다. 특히 낮/밤의 온도 차가 11~25℃일 때, 85%이상의 발아율을 보였고, 그 이상의 온도차이가 나면 발아율은 감소하는 경향을 보였다. T₅₀은 온도차가 증가할수록 감소하여, 발아가 균일하며 빨라지는 것을 확인 할 수 있었다. 또한 GA₃ 처리시 생리적 휴면 타파가 진행되어 발아율(79.8%~100%)과 발아속도가 증가(T₅₀ 감소)함을 확인하였으나, GA₃ 100mg·L^-1 이상의 농도에서는 무처리(21.2m)에 비하여 유근의 길이 생장을 0.11~6.88mm로 지연시켰다.

• 한국환경복원기술학회지
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• 제9권4호
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• pp.24-35
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• 2006

The vascular plants of the studied area was listed 384 taxa of 90 families, 234 genera, 331 species, 1 subspecies, 45 varieties and 7 forms. The flora of each reservoir, 285 taxa of 83 families, 190 genera, 247 species, 1 subspecies, 30 varieties, 7 forms in Osongjae, 117 taxa of 39 families, 87 genera, 100 species, 17 varieties in Ogongjae, 83 taxa of 30 families, 67 genera, 73 species, 8 varieties, 2 forms in Shinyongjae and 86 taxa of 33families, 70 genera, 75 species, 1 subspecies, 10 varieties in Eodujae. Based on the list of the rare plants by the Forest Research Institute, 3 taxa were recorded in the studied areas; Magnolia kobus (Preservation priority order : No. 187), Penthorum chinense (No. 144), Prunus yedoensis (No. 110) and based on the list of Korean endemic plants, 4 taxa were recorded; Populus tomentiglandulosa, Clematis trichotoma, Prunus yedoensis, Paulownia coreana. Specific plant species by floral region were total 15 taxa; Prunus yedoensis in class V, 2 taxa (Carex idzuroei, Magnolia kobus) in class IV, 2 taxa (Monochoria korsakowi, Poncirus trifoliata ) in class III, 10 taxa (Sagittaria aginashi, Salix glandulosa, Clematis brachyura, Nymphoides peltata, etc.) in class I. The naturalized plants in this site were 13 families, 29 genera, 43 species, 1 varieties, 44 taxa and naturalization rate was 11.5% and based on the list of an ecosystem disturbance plants, 3 taxa were recorded in the studied areas : Paspalum distichum, Solanum carolinense, Ambrosia artemisiifolia var. elatior. The hydrophytes was listed 31 taxa of 15 families, 20 genera, 29 species, 2 varieties and Eleocharis mamillata var. cyclocarpa, Penthorum chinense, Soirodela polyrhiza were recorded in Osongjae, Sagittaria aginashi, Eleocharis kuroguwai, Scirpus juncoides, Scirpus triqueter, Monochoria korsakowi, Monochoria vaginalis var. plantaginea were recorded in Ogongjae. Besides, Scirpus fluviatilis, Trapa pseudoincisa were recorded in Shinyongjae and Scirpus triangulatus, Nymphoides peltata, Myriophyllum verticillatum were recorded in Eodujae. This site is located in the urban area and ecosystem-disturbing wild animal and plant has been imported here, therefore ecosystem has been disturbed more and more. The wetland here rapidly has changed into upland, so it needs conservation measures through long-term monitoring.

• 화훼연구
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• 제18권2호
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• pp.117-124
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• 2010

The purpose of this study was to create an artificial wetland in rooftop greening. We monitored species and changes of flora in wetland and rooftop greening. As shown the consideration and possibility of supplying the artificial wetland in rooftop greening through flora in wetland, this study tried to find methods to create a efficient flora space. This results are listed as belows. The species were applied to artificial wetland in rooftop greening and come up to 'General standard for selecting plants' among hydrophyte in wetlands. The plants of Potentilla kleiniana, Penthorum chinense, Scirpus radicans, Scirpus triqueter, Veronica undulata, Mentha arvensis var. piperascens, Salvia plebeian, Sagittaria aginashi, Aneilema keisak, Stachys riederi, Alisma canaliculatum, Eclipta prostrata, Sparganium stoloniferum turned out an appropriate species. This research was expected to create a various environment and component of species by introducing many types of plants in ecological wetland on rooftop greening.

• 환경생물
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• 제30권2호
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• pp.107-120
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• 2012

본 조사는 유구천의 수생식물과 수변계류식물의 목록을 작성하고 관속식물의 다양성을 연구하기 위해 문헌조사를 포함하여, 2009년 8월부터 2010년 9월까지 총 4회에 걸쳐 7개 지점을 조사하였다. 7개 지점의 조사를 통해 유구천의 관속식물은 88과 229속 290종 3아종 30변종 5품종 328분류군으로 확인되었다. 조사지역 내의 한국 특산식물은 개나리(Forsythia koreana (Rehder) Nakai), 오동나무 (Paulownia coreana Uyeki), 병꽃나무 (Weigela subsessilis (Nakai) L.H. Bailey) 등 3분류군이 확인되었다. 산림청 지정 희귀 및 멸종위기 식물은 낙지다리 (Penthorum chinense Pursh), 땅나리 (Lilium callosum Siebold & Zucc.), 꽃창포(Iris ensata var. spontanea (Makino) Nakai), 창포 (Acorus calamus L) 등 4분류군이 확인되었다. 식물구계학적 특정종은 총 27분류군이 확인되었는데, 그 중 중요한 의미를 가지는 III~V등급종은 V등급종이 2분류군, IV등급종이 2분류군, III등급종이 9분류군으로 총 13분류군이 발견되었다. 귀화식물은 10개과 27분류군이 확인되었으며, 이는 조사된 전체 목록 중 약 8.3%를 차지하는 것으로 확인되었다. 유구천은 마을이 하천을 따라 발달하고, 도로나 농경지 등에 따라 인간의 간섭이 지속적으로 증가 할 것으로 여겨지며 이에 따라 귀화식물의 유입도 증가 할 것으로 판단되어진다. 조사지점별 식물들의 집괴분석과 좌표 결정분석 결과 유구천 본류형, 소하천형, 저수지형 등 3개 모둠으로 구별되었다.

• Journal of Plant Biology
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• 제28권1호
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• pp.29-44
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• 1985

돌나무과(Crassulaceae)에 속하는 식물 24종을 대상으로 비정상기공의 유형과 종간 출현빈도를 조사한 결과는 다음과 같다. 비정상기공의 유형은 helico-eumesogenous stomata와 anomo-hemimesoperi-genous stomata에서 총 19개 유형이 관찰되었고 이들 중에서 single guard cell, single-aborted guard cell 및 aborted stomata의 3개 유형은 19∼24종에서 발견되어 가장 높은 빈도를 나타내었고 다른 14개 유형은 1∼5종에서만 발견되어 가장 낮은 빈도를 나타내었다. 종에 따른 비정상기공의 출현빈도는 총 19개 유형중 꿩의 비름(Sedum alboroseum), 낙지다리(Penthorum chinense) 및 돌나물(Sedum sarmentosum)의 3종에서 9∼14개 유형이 발견되어 가장 높은 빈도를 나타내었으며, 여타 종은 7개 유형 이하의 낮은 빈도를 나타내었다. 비정상기공의 유형중 ballooning guard cell, cross cleaved stomata 및 fused pore stomata의 3개 유형은 지금까지 문헌상에서 발표되지 않았고 저자등에 의하여 최초로 발견되었다. 비정상기공은 정상기공의 퇴화의 결과이거나 비정상적 발생의 결과로 형성된다는 것을 관찰할 수 있었다.

• 한국환경복원기술학회지
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• 제14권2호
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• pp.59-81
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• 2011

The flora of the Sinpyeong and Churyeong stream were listed 324 taxa; 87 families, 227 genera, 289 species, 1 subspecies, 31 varieties and 3 forms. The Sinpyeong stream were 249 taxa and Churyeong stream were 221 taxa. Based on the rare plants (IUCN) by the Korea Forest Service and Korea National Arboretum were recorded; Penthorum chinense (Crassulaceae), etc. and endemic plants, 6 taxa; Weigela subsessilis (Caprifoliaceae), Lycoris flavescens (Amarylidaceae), etc. Based on the specific plants by floral region were total 12 taxa (3.7% of all 324 taxa of flora); Monochoria korsakowi (Pontederiaceae), etc. in class III. 10 taxa (Salix glandulosa (Salicaceae), Ulmus parvifolia (Ulmaceae), Impatiens noli-tangere (Balsaminaceae), Grewia biloba var. parviflora (Tiliaceae), Nymphoides peltata (Gentianaceae), Actinostemma lobatum (Cucurbitaceae), Cirsium pendulum (Compositae), Microstegium japonicum (Gramineae), etc.) in class I. Based on the naturalized plants, 51 taxa and ecosystem disturbing wild plants, 6 taxa (Rumex acetosella, Sicyos angulatus, Aster pilosus, Ambrosia artemisiaefolia, Hypochaeris radicata, Paspalum distichum var. distichum) and naturalization rate was 15.7% of all 324 taxa of flora, urbanization index was 18.8% of all 271 taxa of naturalized plants. Based on the hydrophytes, 23 taxa and emergent hydrophytes were 17 taxa, floating-leaved hydrophytes were 3 taxa, submergent hydrophytes were 2 taxa, free-floating hydrophytes was Spirodela polyrhiza.