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http://dx.doi.org/10.15230/SCSK.2019.45.1.87

Analytical Method Development of (-)-Epicatechin gallate in Penthorum chinense Pursh Extract using HPLC  

Kwon, Jin Gwan (Gyeonggido Business and Science Accelerator)
Jung, Yeon Woo (Gyeonggido Business and Science Accelerator)
Seo, Changon (Gyeonggido Business and Science Accelerator)
Hong, Seong Su (Gyeonggido Business and Science Accelerator)
Choi, Chun Whan (Gyeonggido Business and Science Accelerator)
Lee, Ji Eun (Gyeonggido Business and Science Accelerator)
Shin, Hyun Tak (DMZ Botanic Garden, Korea National Arboretum)
Jung, Su Young (DMZ Botanic Garden, Korea National Arboretum)
Kim, Jin Kyu (Gyeonggido Business and Science Accelerator)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.45, no.1, 2019 , pp. 87-93 More about this Journal
Abstract
This study attempted to eatablish a High Performance Liquid Chromatography (HPLC) analysis method for the determination of (-)-epicatechin gallate as a part of the quality control for the development of functional cosmetic materials from Penthorum chinense Pursh. HPLC was performed on a Unison $US-C_{18}$ column ($4.6{\times}250mm$, $5{\mu}m$) with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and methyl alcohol at a flow rate of 1.0 mL/min at $30^{\circ}C$. The analyte was detected at 280 nm. The HPLC method was performed in accordance with the International Conference on Harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation were 0.11 and 0.33 mg/mL, respectively. Calibration curves showed good linearity ($r^2$ > 0.9999), and the precision of analysis was satisfied (less than 0.6%). Recoveries of quantified compounds ranged from 99.51 to 101.92%. This result indicates that the established HPLC method is very useful for the determination of marker compound in P. chinense Pursh extracts.
Keywords
(-)-epicatechin gallate; Functional cosmetic; HPLC; Penthorum chinense Pursh; Validation;
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