• Applied Biological Chemistry
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• 제31권1호
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• pp.33-37
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• 1988

김치에서 분리한 Pediococci를 동정한 결과 Pediococcus pentosaceus, Pediococcus acidilactici 및 Pediococcus halophilus의 세종으로 밝혀졌다. 이 Pediococci의 미생물 생육저해 작용을 조사한 결과 P. pentosaceus는 모두 Streptococcus faecalis와 Pseudomonas sp., P20에 대하여 생육저해작용을 나타내었다. P. acidilatici는 S. faecalis와 Vibrio parahaemolyticus의 생육을 저해하였으나 P. halophilus는 4종의 test organism에 대하여 저해작용이 없었다. 이들 Pedicocci는 균주에 따라 한개로 부터 일곱개의 plasmid DNA를 가지고 있으며 그 분자량은 약 1 Mdal에서부터 약 60 Mdal 사이의 여러 크기의 plasmid DNA가 존재함이 밝혀졌다.

• Journal of Microbiology and Biotechnology
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• 제13권3호
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• pp.429-436
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• 2003

Pediocin K1 is a bacteriocin produced by Pediococcus sp. KCA 1303-10, isolated from traditionally fermented flatfish in Korea. Pediocin K1-dependent antibiosis and pediocin K1-independent antibiosis against Listeria monocyrogenes were investigated by comparing antibiosis potential of the ped＋ wild-type strain of Pediococcus sp. KCA1303-10 with that of the ped- mutant strain in 3 different media at 3 different temperatures. In the synthetic MRS-APT medium, bacteriocin (pediocin K1)-dependent antibiosis (BDA) acted as the major driving force of overall antibiosis at the initial stage before the pH of the media was not sufficiently lowered, while bacteriocin-independent antibiosis (BIA) took over the major role at the late stage of antibiosis by killing otherwise resistant cells in the modium. The role of BDA increased as the temperature of the system decreased. The antibiosis potential of BDA among the overall antibiosis of Pediococcus against Listeria at $37^{\circ}C$ was calculated as 46%, and as 75% at $25^{\circ}C$. In the skim milk medium, antibiosis of Pediococcus against Listeria was weakened more than 4 log cycles compared to that of the synthetic medium; however, BDA worked as the main antibiosis force regardless of the culturing temperature in the skim milk medium. In the bean soup medium, BDA also worked as the major killing mechanism against Listeria, but BIA played as another suppressing mechanism against otherwise pediocin-resistant Listeria population. These results suggest that a large portion of the inhibitory action of the ped+Pediococcus sp. KCA1303-10 was attributable to the bacteriocin produced by the strain and that viable Pediococcus sp. KCA1303-10 was superior to the purified bacteriocin in suppressing the occurrence of the bacteriocin-resistant Listeria monocytogenes in food systems.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.161-168
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• 2010

In this study, the metabolic activities of five strains of Pediococcus spp., in terms of the quantities they produced of lactic acid, hydrogen peroxide, exopolysaccharides, and proteolytic activity, were determined. Lactic acid levels produced by these strains were found to be in the range of 2.5-5.6 mg/ml. All strains produced hydrogen peroxide. The P. pentosaceus Z13P strain produced the maximum amount (0.25 mg/ml) of proteolytic activity. Exopolysaccharide (EPS) production by the Pediococcus strains during growth in MRS (de Man, Rogosa, and Sharpe) medium was in the range 25-64 mg/l. The susceptibility of 10 different antibiotics against these strains was also tested. All strains were found to be resistant to amoxicillin, gentamicin, and vancomycin. Antimicrobial effects of the Pediococcus spp. on pathogens were also determined by an agar diffusion method. All of the strains were able to inhibit L. monocytogenes. The tolerance of the strains to low pH, their resistance to bile salts of strains, and their abilities to autoaggregate and coaggregate with L. monocytogenes were also evaluated.

• Preventive Nutrition and Food Science
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• 제17권3호
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• pp.217-222
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• 2012

This study was carried out to investigate the isolation and characterization of lactic acid bacteria (LAB) from naturally fermented sauce-type kimchi. Sauce-type kimchi was prepared with fresh, chopped ingredients (Korean cabbage, radish, garlic, ginger, green onion, and red pepper). The two isolated bacteria from sauce-type kimchi were identified as Pediococcus pentosaceus and Lactobacillus brevis by 16S rDNA sequencing and tentatively named Pediococcus sp. IJ-K1 and Lactobacillus sp. IJ-K2, respectively. Pediococcus sp. IJ-K1 was isolated from the early and middle fermentation stages of sauce-type kimchi whereas Lactobacillus sp. IJ-K2 was isolated from the late fermentation stage. The resistance of Pediococcus sp. IJ-K1 and Lactobacillus sp. IJ-K2 to artificial gastric and bile acids led to bacterial survival rates that were 100% and 84.21%, respectively.

• 한국축산식품학회지
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• 제43권1호
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• pp.170-183
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• 2023

This study evaluated the effects of milk fermented with Pediococcus acidilactici strain BE and Pediococcus pentosaceus strain M103 on diabetes in rats (Rattus norvegicus). The bacteria were separately used as starter cultures for milk fermentation, and the products were then fed to diabetic rats for 15 days. Blood glucose levels, immunohistochemical and histological indicators, lipid profiles, and total lactic acid bacterium counts were evaluated before and after treatment. The administration of milk fermented with P. acidilactici strain BE reduced blood glucose levels from 410.27±51.60 to 304.07±9.88 mg/dL (p<0.05), similar to the effects of metformin (from 382.30±13.39 mg/dL to 253.33±40.66 mg/dL, p<0.05). Increased insulin production was observed in diabetic rats fed milk fermented with P. acidilactici strain BE concomitant with an increased number and percentage area of immunoreactive beta-cells. The structure of insulin-producing beta-cells was improved in diabetic rats fed milk fermented with P. acidilactici strain BE or metformin (insulin receptor substrate scores of 5.33±0.94 and 3.5±0.5, respectively). This suggests that the administration of milk fermented with P. acidilactici BE potentially reduces blood glucose levels and improves pancreatic beta-cell function in diabetic rats.

• 한국미생물·생명공학회지
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• 제34권1호
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• pp.74-77
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• 2006

A lactic acid bacterial strain resistant to several antibiotics such as oxytetracycline, tylosin, neomycin and sulfathiazole, which have been often used as a therapeutic agent in livestock, was isolated from the porcine gastrointestinal tract. The isolate YB-55 was identified as belonging to the genus Pediococcus with the highest similarity to P. pentosaceus on the basis of its 16S rRNA sequence and biochemical properties. The isolated strain showed viability of over 85% at pH 3.0 and was resistant to bile salt. The strain produced lactic acid of 12.3 g/L by jar fermentation and maintained its viability in the presence of antibiotics at dosage related therapeutic effect, suggesting P. pentosaceus YB-55 may of ffr potential as a probiotics for livestock.

• Applied Biological Chemistry
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• 제29권2호
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• pp.207-211
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• 1986

김치 발효 경과에 따라 젖산균을 분리하여 E. coli와 Streptococcus faecalis, Lactobacillus bulgaricus에 대해 생육 저해능력이 있는 균주를 screening하였다. 젖산균중 생육 저해균의 비율을 조사한 결과 이들은 2주의 발효기간 중 중기에 가장 많이 나타났으며 대부분 Pediococcus cerevisiae로 동정하였다. 이 P. cerevisiae의 생육억제 작용은 열에 불안정하고 단백분해 효소의 작용을 받지 않는 물질에 의한 것이며 이 물질은 배지의 pH를 중성으로 유지했을 때는 산성인 경우보다 생성이 저하되는 것으로 나타났다.

• 동아시아식생활학회지
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• 제5권3호
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• pp.309-315
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• 1995

In this study we isolate lactic acid bacteria from kimchi, which were identified as pediococcus pentosaceus and Lactobacillus bevis by using API 50 CHL Kit, some morphological and physiological tests, The water extract of green tea(50, 100, 500, 1000ppm) inhibieted the growth of pediococcus pentosaceus and Lactobacillus brevis in broth system. Furthermore, their time were prolonged to about 4hours at 500 to 1000ppm. The leakage amount of intracellular materials and Mg++ in tested bacteria cells increased with increasing concentration of green tea water extract.

• 한국미생물·생명공학회지
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• 제23권3호
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• pp.359-364
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• 1995

Two lactic strains, Leuconostoc mesenteroides Lu5 and Pediococcus pentosaceus P1 isolated from Kimchi, were used to determine the optimum conditions for protoplast formation and regeneration. The maximum protoplast formation rate was obtained with both strains at early exponential growth phase and decreased rapidly during growth phase. For P. pentosaceus P1, 30 $\mu$g/ml of lysozyme treatment was sufficient to obtain over 90% of protoplast formation and 300 $\mu$g/ml for L. mesenteroides Lu5, showing great difference in sensitivity of these strains to lysozyme. For both strains, best results were obtained at pH 7, using 0.5 M sucrose as osmotic stabilizer. For regeneration of protoplast, the highest regeneration rate was obtained after 15 minutes of lysozyme treatment and declined drastically with prolonged digestion.

• 한국미생물·생명공학회지
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• 제37권3호
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• pp.238-242
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• 2009

분자생물학적 방법에 의한 김치유산균 연구의 진행으로 Leuconostoc, Lactobacillus, Weissella 속 유산균의 김치발효 과정 중 생육에 대한 새로운 결과들이 도출되었지만, Pediococcus속의 생육에 대한 새로운 결과는 거의 보고되지 않았다. 본 연구에서는 김치에 존재하는 Pediococcus 속 유산균의 존재에 대한 재조명을 위하여 ampicillin (A)의 첨가로 pediococci의 선발 유효성이 향상된 것으로 보고된 pediococci selective medium(PSM)+A를 이용하여 pediococci 선택적 검출의 유효성을 검토한 결과, 기존에 보고된 결과와는 달리 Pediococcus pentosaceus 외에도 leuconostocs, Lactobacillus casei, Lactobacillus curvatus, Oenococcus oeni, Streptococcus thermophilus에 대한 생육저해가 발생하지 않았다. PSM+A 한천배지를 이용하여 김치에 생육하는 미생물을 검출한 경우, leuconostocs, P. pentosaceus, Weissella koreensis, Lb. curvatus, Lactobacillus brevis, Lactobacillus sakei가 생육하여 김치와 같은 발효침채류의 발효에 관여하는 pediococci의 선택적 선발에는 PSM+A가 유효하지 않은 것으로 확인되었다.