• 상하수도학회지
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• 제32권2호
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• pp.153-158
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• 2018

In this study, effects of nutrient and inorganic carbon on single cell emergence during the cultivation of microalgae were observed using colonial green algae, Pediastrum duplex. The concentration of inorganic carbon had significant effect on single cell emergence and its growth, but nitrogen and phosphorus concentration showed minor effects. According to P. duplex cultivation experiment, single cell started to be emerged around 500~750 mg-C/L of inorganic carbon concentration and it was bloomed dramatically at the higher values. And growth of P. duplex was started to be surpressed at the single cell formation concentration. From the results, it could be said that when we operate the microalgae systems for cultivation/harvesting or wastewater treatment, in order to avoid single cell formation, inorganic carbon should be maintained to the proper level.

• Fisheries and Aquatic Sciences
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• 제13권1호
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• pp.18-25
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• 2010

Interest in plant-derived food additives has increased recently, with efforts to identify natural antioxidant sources to replace synthetic antioxidants. This study evaluated the antioxidant effects of organic solvent fractions of 80% methanol extracts from Pediastrum duplex and Dactylococcopsis fascicularis microalgae. Among the solvent fractions tested, the ethyl acetate and n-hexane fractions from P duplex effectively scavenged 79.8% and 74.5% of DPPH free radicals, respectively. The chloroform fraction from D. fascicularis showed the strongest $H_2O_2$ scavenging ability (49.7%). The greatest scavenging of hydroxyl radicals (73.1%) was exhibited by the 80% methanol extract from P duplex. Aqueous residue and ethyl acetate fraction from P duplex provided the strongest nitric oxide scavenging (57.7%) and metal chelating effect (82.1%), respectively. Chloroform and ethyl acetate fractions from P duplex and n-hexane fraction from D. fascicularis exhibited significantly greater inhibition of lipid peroxidation than the commercial antioxidants. These results suggested that P duplex and D. fascicularis microalgae having potential anti oxidative compounds with various properties could be utilized in the food and pharmaceutical industries.

• ALGAE
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• 제24권3호
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• pp.169-177
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• 2009

In this study, we focused on natural water-soluble antioxidants from fresh water microalgae, Pediastrum duplex and Dactylococcopsis fascicularis from Jeju Island, Korea. They were prepared by enzymatic digestion using five carbohydrases (Viscozyme, Celluclast, AMG, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme and Alcalase), and the potential antioxidant activity of each was assessed. All enzymatic digests from P. duplex showed significant DPPH scavenging effects. Termamyl (60.6%) digest from P. duplex possessed the highest effects on hydrogen peroxide scavenging. Celluclast (58.1%) and Kojizyme digests (56.9%) from D. fascicularis possessed higher effects on superoxide anion radical scavenging. All enzymatic digests exhibited significant effects on both NO· scavenging and metal chelating. Lipid peroxidation was significantly in inhibited Viscozyme, Termamyl and Kojizyme digests from P. duplex and Ultraflo, Protamex, Kojizyme and Alcalase digests from D. fascicularis. These data suggest that enzymatic digests of the fresh water microalgae, P. duplex and D. fascicularis might be valuable sources of antioxidant which can be applied in food and pharmaceutical industry.

• 한국환경과학회지
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• 제4권3호
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• pp.167-176
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• 1995

A study on phytoplankton community was carried out from June, 1992 to May, 1993 at selected stations in Hoedong Reservoir. The phytoplankton are identified as 176 taxa including 5 phylum, 67 genera. The important species in this reservoir were Asterionella formosa, C meneghiniana, Dictyosphaerium plilchellum, Fragilaria crotonensis, Melosira distans, M grantulata, M. granulata var. angustissima, M. granulate var. angntissima f. spirdis, Micractinium pussillum, Microcystis aeruginosa, Pundorina morn, Pediastrum boryanum, P. duplex, Peridinium sp., Scenedesmus quadriauda, Synedra acus, S. rumpens and S. ulna. The causative species of water bloom were identified as Microcystis aeruginsa, Trahellomonas hispida, Ceratium hirwdinella, Peridinium sp., Melosira italica, Staurastrum dorsidentiferum var. onatum in the area. During the study periods standing crcps of phytoplankton were maximum in August, 1992 and minimum in December 1992. The species dominance index and diversity index were ranged 24.7-99.9, 0.001-3.06, respectively. Key Words : phytoplankton community, causative species of water bloom, standing crops. dominance index, species diversity index.

• 생태와환경
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• 제45권1호
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• pp.21-33
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• 2012

동강 수계의 식물플랑크톤 군집 구조를 알아보기 위하여 2008년 5월부터 2009년 4월까지 22개 지점에서 총 4 회 조사를 실시하였다. 동강수계에서 출현한 식물플랑크톤은 총 159종으로서 7강 58속 113종 21변종 25미동정종으로 구성되어 있으며, 규조강이 90종류로 전체 중 56.6%로 가장 높은 점유율을 나타냈고, 그 다음으로 녹조강이 47종류로 29.6%의 점유율을 나타냈으며 남조강이 17종으로 10.7%, 유글레나강이 2종으로 1.3%, 황색편모조강, 갈색편모조강, 와편모조강이 각각 1종 0.6% 순으로 나타났다. 조사기간 모든 시기 20정점 이상에서 출현한 보편종은 Cocconeis placentula, Cymbella minuta, Diatoma vulgare, Melosira varians, Navicula cryptocephala, Scenedesmus acuminatus v. acuminatus 등 6종이었다. 현존량은 86 cells $mL^{-1}$~1,467 cells $mL^{-1}$의 범위로 나타났고, 점유율이 10% 이상인 우점종은 Achnanthes minutissima, Asterionella formosa, Aulacoseira ambigua, Cocconeis placentula, Coelastrum microporum, Cyclotella sp., Cymbella affinis, C. minuta, C. tumida, Diatoma vulgare, Fragilaria capucina, F. construens, F. crotonensis, Gomphonema affine, G. clevei, Melosira varians, Merismopedia elegans, Navicula cryptocephala N. pupula, Nitzschia tryblionella, Oscillatoria anna, O. limosa O. tenuis v. tenuis, Pediastrum duplex v. reticulatum, Phormidium tenue, Scenedesmus acuminatus v. acuminatus, S. acutus v. acutus, S. ecornis v. ecornis, S. quadricauda v. quadricauda, Spirogyra sp., Stigeoclonium sp., Synedra acus, S. ulna, Ulothrix sp. 등 34종이었는데, 가장 중요한 종은 90개 시료 중 52개에서 우점종으로 나타난 Cymbella minuta이었다 종다양성지수는 1.58~3.10, 균등도지수는 0.51~0.95, 우점도지수는 0.22~0.74의 범위로 연구지역이 상당히 안정된 것으로 조사되었다. 본 조사지역의 상류정점의 식물플랑크톤 군집구조는 도암호에서 나오는 방류수에 의해 영향을 받는 것으로 사료된다.

• ALGAE
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• 제18권4호
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• pp.255-262
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• 2003

Ecological characteristics of phytoplankton communities were investigated biweekly at 6 stations from Feb. 2001 to Feb. 2002 at the lower part of the Han River. During the study, a total of 267 taxa which were composed of 114 green algae, 104 diatoms, 22 blue-green algae, 13 chrysophytes and 2 dinoflagellates were observed. The standing crops of phytoplankton communities were ranged from 1.30 ${\times}10^6$ cells ${\cdot}l^{-1}$ to 37.47 ${\times}10^6$ cells ${\cdot}l^{-1}$. The dominant species were 11 taxa including Pediastrum duplex, Scenedesmus quadricauda in green algae, Osillatoria limosa, Microcystis aeruginosa, Merisomorpedia sp., Phormidium sp. in blue-green algae, and Asterionella gracillima, Aulacoseira granulata, Fragilaria crotonensis, Stephanodiscus hantzschii f. tenuis, Synedra acus in diatoms. Asterionella gracillima and Stephanodiscus hantzschii f. tenuis were abundant at every station from winter to spring. Stephanodiscus hantzschii f. tenuis occupied the highest dominant ratio and standing crop. From summer to autumn, dominant species were Aulacoseira granulata at all stations and blue-green algae such as Osillatoria limosa, Microcystis aeruginosa, Merisomorpedia sp. at some stations. These 4 species were mostly present at the lower stations 4, 5, 6 and very few at the upper stations 1, 2, 3.

• ALGAE
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• 제18권2호
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• pp.145-156
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• 2003

Seasonal investigations were conducted to determine the major aquatic environmental factors and the variation of phytoplankton in Pyeongtaek Reservoir in March, June, September, and December 2000. Heavy rainfall mainly occurs from late June to mid-September, and water quality of reservoir was high in the influent zone of stream and riverine zone of reservoir. The biomass of phytoplankton was related to aquatic environmental factors. In particular, its value increased where nutrient concentration was high. Likewise, the increase of turbidity was found to have anthropogenic effects on the varying quantity of phytoplankton. The phytoplankton composition in quantitative survey identified into 43 genera and 71 species. Species numbers of Bacillariophyceae, Cyanophyceae, and Chlorophyceae accounted for 17%, 15%, and 49%, respectively, with the remainder constituting less than 3-7%. The distribution of such phyla also significantly varied according to seasons, accounting for 25%, 37%, 61%, and 14% in March, June, September, and December, respectively. Bacillariophyceae and Chlorophyceae were observed throughout the year, while Cyanophyceae proliferated in June and September. Euglenophyceae and Dinophyceae were prevalent in March and September, while Cryptophyceae occurred in March and December. The succession trend of phytoplankton showed the maximum cell density was followed by Bacillariophyceae (6.8$\times$$10^3$ cells ${\cdot}$ml)$\rightarrow$ Chlorophyceae (3.7$\times$$10^3$ cells ${\cdot}$ml)$\rightarrow$Cyanophyceae (1.3$\times$$10^4$ cells ${\cdot}$ml)$\rightarrow$Cryptophyceae (1.2$\times$$10^3$ cells ${\cdot}$ml). The cell density was the highest in the upstream. Dominant species were composed of Aulacoseira ambigua, Stephanodiscus hantzschii f. tenuis of Bacillariophyceae, Anabaena spiroides var. crassa, Microcystis aeruginosa, Oscillatoria amphibia of Cyanophyceae, Actinastrum hantzschii var. fluviatile, Pediastrum duplex var. reticulatum of Chlorophyceae, Euglena gracilis, Trachelomonas spp. of Euglenophyceae, and Chroomonas spp., Cryptomonas spp. of Cryptophyceae. As a results, seasonal variation of phytoplankton in Pyeongtaek Reservoir was evident in spite of inflow the high concentration of nutrients from watershed streams, because hydrological control and anthropogenic disturbance in reservoir were found to have major effects on the retention time of water.