• Title/Summary/Keyword: PcoA

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Analysis of gut bacterial diversity and exploration of cellulose-degrading bacteria in xylophagous insects (목질섭식곤충의 장내 세균 다양성 분석 및 섬유소 분해균 탐색)

  • Choi, Min-Young;Ahn, Jae-Hyung;Song, Jaekyeong;Kim, Seong-Hyun;Bae, Jin-Woo;Weon, Hang-Yeon
    • Korean Journal of Microbiology
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    • v.51 no.3
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    • pp.209-220
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    • 2015
  • In this study, gut bacterial communities in xylophagous insects were analyzed using the pyrosequencing of 16S rRNA genes for their potential biotechnological applications in lignocelluloses degradation. The result showed that operational taxonomic units (OTUs), species richness and diversity index were higher in the hindgut than in the midgut of all insect samples analyzed. The dominant phyla or classes were Firmicutes (54.0%), Bacteroidetes (14.5%), ${\gamma}-Proteobacteria$ (12.3%) in all xylophagous insects except for Rhinotermitidae. The principal coordinates analysis (PCoA) showed that the bacterial community structure mostly clustered according to phylogeny of hosts rather than their habitats. In our study, the two carboxymethyl cellulose (CMC)-degrading isolates which showed the highest enzyme activity were most closely related to Bacillus toyonensis $BCT-7112^T$ and Lactococcus lactis subsp. hordniae $NCDO\;2181^T$, respectively. Cellulolytic enzyme activity analysis showed that ${\beta}-1,4-glucosidase$, ${\beta}-1,4-endoglucanase$ and ${\beta}-1,4-xylanase$ were higher in the hindgut of Cerambycidae. The results demonstrate that xylophagous insect guts harbor diverse gut bacteria, including valuable cellulolytic bacteria, which could be used for various biotechnological applications.

Influence of metabolizable energy on blood biomarkers in duck under heat stress (대사에너지가 열 스트레스에 노출된 오리의 혈액 생체지표에 미치는 영향)

  • Sim, J.B.;Choi, D.H.;Kim, C.R.;Park, B.S.
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.1
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    • pp.132-141
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    • 2017
  • The objective of this study was to determine the influence of dietary metabolic energy (ME) on blood parameters in duck under heat stress. A total of 240 meat ducks Cherry valley (Anas platyrhynchos) were assigned into four treatment groups with a randomized block design for 42 days. The four treatments were: ME 2900 kcal/kg, ME 3000 kcal/kg, ME 3100 kcal/kg, and ME 3200 kcal/kg. Blood lipid profiles was higher in ME 2900 but lower in ME 3100 and ME 3200 than that of ME 3000 (p < 0.05). Blood aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were higher in ME 3100 and ME 3200 compared those in ME 3000 (p < 0.05). The blood red cell and platelet profiles were increased in ME 3100 and ME 3200, but reduced in ME 2900 compared to those in ME 3000 (p < 0.05). Among blood electrolytes, chloride ($Cl^-$) concentration was decreased in ME 2900 compared to that in ME 3000. Blood gas $PCO_2$ was reduced in ME 2900 compared to that in ME 3000 (p < 0.05). Blood immunoglobulin (IgG) level was reduced in ME 2900 compared to that in ME 3000 (p < 0.05). Level of stress hormone, corticosterone was increased in ME 2900, but decreased in ME 3100 and ME 3200 compared to that in ME 3000 (p < 0.05).

PD-DESYNC: Practical and Deterministic Desynchronization in Wireless Sensor Networks

  • Hyun, Sang-Hyun;Kim, Geon;Yang, Dongmin
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.13 no.8
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    • pp.3880-3899
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    • 2019
  • Distributive desynchronization algorithms based on pulse-coupled oscillator (PCO) models have been proposed for achieving collision-free wireless transmissions. These algorithms do not depend on a global clock or infrastructure overheads. Moreover, they gradually converge to fair time-division multiple access (TDMA) scheduling by broadcasting a periodic pulse signal (called a 'firing') and adjusting the next firing time based on firings from other nodes. The time required to achieve constant spacing between phase neighbors is estimated in a closed form or via stochastic modeling. However, because these algorithms cannot guarantee the completion of desynchronization in a short and bounded timeframe, they are not practical. Motivated by the limitations of these methods, we propose a practical solution called PD-DESYNC that provides a short and deterministic convergence time using a flag firing to indicate the beginning of a cycle. We demonstrate that the proposed method guarantees the completion of desynchronization within three cycles, regardless of the number of nodes. Through extensive simulations and experiments, we confirm that PD-DESYNC not only outperforms other algorithms in terms of convergence time but also is a practical solution.

Change of End-tidal PCS During Cardiopulmonary Bypass (체외순환시 호기말 이산화탄소압의 변화)

  • 오중환
    • Journal of Chest Surgery
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    • v.25 no.12
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    • pp.1399-1403
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    • 1992
  • The evaluation of the effectivess of ongoing cardiopulmonary resucitation efforts is dependent on the commonly used methods, such as the presence of femoral or carotid artery pulsations, arterial blood gas determinations, peripheral arterial pressure and intracardiac pressure monitoring. But recent studies suggest that end-tidal carbon dioxide tension serves as a non-invasive measurement of pulmonary blood flow and therefore cardiac output under constant ventilation. A prospective clinical study was done to determine whether end-tidal carbon dioxide monitoring in open heart surgery under cardiopulmonary bypass could be used as a prognostic indicator of bypass weaning. We monitored end-tidal PCO2 values continuously during cardiopulmonary bypass in 30 patients. "Ohmeda 5210 CO-2 monitor" under infrared absorption method were incorperated into the ventilator circuit by means of a side point adaptor between endotracheal tube and ventilator tubing. 18 patients[Group I ] were res-ucitated from partial bypass followed by aorta cross clamp off and 12 patients[Group II ] from aorta cross clamp off followed by partial bypass. But there was no difference between two groups[p>0.05]. The value of end-tidal carbon dioxide tension during ventricular fibrillation or nearly arrest state was 6.6$\pm$2.9 mmHg, and at the time of spontaneous beating was 19.3$\pm$5.6 mmHg[Mean$\pm$Standard deviation], In conclusion end-tidal carbon dioxide tension monitoring provides clinically useful, continous, noninvasive and supplementary prognostic indicator during cardiopulmonary bypass weaning procedures.rocedures.

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State of Health Estimation for Lithium-Ion Batteries Using Long-term Recurrent Convolutional Network (LRCN을 이용한 리튬 이온 배터리의 건강 상태 추정)

  • Hong, Seon-Ri;Kang, Moses;Jeong, Hak-Geun;Baek, Jong-Bok;Kim, Jong-Hoon
    • The Transactions of the Korean Institute of Power Electronics
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    • v.26 no.3
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    • pp.183-191
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    • 2021
  • A battery management system (BMS) provides some functions for ensuring safety and reliability that includes algorithms estimating battery states. Given the changes caused by various operating conditions, the state-of-health (SOH), which represents a figure of merit of the battery's ability to store and deliver energy, becomes challenging to estimate. Machine learning methods can be applied to perform accurate SOH estimation. In this study, we propose a Long-Term Recurrent Convolutional Network (LRCN) that combines the Convolutional Neural Network (CNN) and Long Short-term Memory (LSTM) to extract aging characteristics and learn temporal mechanisms. The dataset collected by the battery aging experiments of NASA PCoE is used to train models. The input dataset used part of the charging profile. The accuracy of the proposed model is compared with the CNN and LSTM models using the k-fold cross-validation technique. The proposed model achieves a low RMSE of 2.21%, which shows higher accuracy than others in SOH estimation.

Assessment of Genetic Diversity and Population Structure on Kenyan Sunflower (Helianthus annus L.) Breeding Lines by SSR Markers

  • Mwangi, Esther W.;Marzougui, Salem;Sung, Jung Suk;Bwalya, Ernest C.;Choi, Yu-Mi;Lee, Myung-Chul
    • Korean Journal of Plant Resources
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    • v.32 no.3
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    • pp.244-253
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    • 2019
  • In crop breeding program, information about genetic dissimilarity on breeding resources is very important to corroborate genealogical relationships and to predict the most heterozygotic hybrid combinations and inbred breeding. This study aimed to evaluate the genetic variation in Kenyan sunflower breeding lines based on simple sequence repeat (SSR). A total of 83 alleles were detected at 32 SSR loci. The allele number per locus ranged from 2 to 7 with an average of 2.7 alleles per locus detected from the 24 sunflower accessions and the average value of polymorphic information contents (PIC) were 0.384. A cluster analysis based on the genetic similarity coefficients was conducted and the 24 sunflower breeding resources were classified into three groups. The principal coordinates (PCoA) revealed 34% and 13.38% respectively, and 47.38% of total variation. It was found that the genetic diversity within the Kenyan sunflower breeding resources was narrower than that in other sunflower germplasm resources, suggesting the importance and feasibility of introducing elite genotypes from different origins for selection of breeding lines with broader genetic base in Kenyan sunflower breeding program.

Analysis of Genetic Diversity and Relationships of Korean Native Black Goat using Microsatellite Markers (초위성체 마커를 이용한 한국 재래 흑염소의 유전적 다양성 및 유연관계 분석)

  • Park, Byeong Kyu;Kim, Yi Seul;Seong, Jiyeon;Kong, Hong Sik
    • Journal of Animal Reproduction and Biotechnology
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    • v.34 no.3
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    • pp.183-189
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    • 2019
  • The aim of this study was to assess the levels of genetic diversity and relationships of Korean native black goat (n = 58), compared with the exotic breed, Boer (n = 97). For the analysis of genetic characterization 11 microsatellite markers (MAF065, INRA063, CSRD247, OarFCB20, SRCRSP5, INRA006, ILSTS008, ILSTS011, INRA005, ILSTS087, SRCRSP8) were genotyped. The number of alleles was observed 3 (INRA005) to 10 (SRCRSP8) each markers. The mean expected and observed heterozygosity (Hexp and Hobs) and polymorphism information content (PIC) for the Korean native black goat breed varied from 0.551 to 0.860, 0.517 to 0.948 and 0.464 to 0.835, respectively. Principal Components Analysis (PCoA) and FCA results showed that Korean native black goat breed was confirmed to be clearly separated from bore breed. These results were scientific evidence that Korean native black goat represents a unique and valuable animal genetic resource.

Studies on the Effects of Haengsoyeum (杏蘇飮) Water Extract on the Plasma Cortisol Concentration in the Rabbit and the Contraction of Isolated Guinea Pig Tracheal Smooth Muscle (행소음전탕액(杏蘇飮煎湯液)이 가토혈장(家兎血漿) COTISOL농도(濃度) 및 $PCO_2,\;CO_2$와 GUINEA PIG의 기관지(氣管支) 평활근(平滑筋)에 미치는 영향(影響))

  • Lee, Sueng-Ho
    • The Journal of Internal Korean Medicine
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    • v.13 no.1
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    • pp.143-155
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    • 1992
  • The following results were obtained from the observation on the change of plasma cortisol concentration in the experiment of intravenous administration of Haengsoyeum Water Extract in the rabbit. And the effects of Haengsoyeum extract on the contractile force of the isolated guinea pig trachea smooth muscle. 1. In intravenous administration the plasma cortisol concentration increased significantly about 1 hours after with a does of 0.2 ml/kg. 2. In intravenous administration the plasma cortisol concentration increased significantly about from 1 to 3 hours after with a does of 0.4 ml/kg. 3. The contractile response of the trachea smooth muscle of isolated guinea pig to histamine $10^{-4}\;M$ was significantly inhibited by Haengsoyeum extract. 4. The contractile response of the trachea smooth muscle of isolated guinea pig to acetylcholine $10^{-4}\;M$ was considerably inhibited by Haengsoyeum extract. 5. The contractile response of the trachea smooth muscle of isolated guinea pig to 5-hydroxytryptamine $10^{-4}\;M$ was inhibited by Haengsoyeum extract.

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Characteristics of Bubble-driven Flow with Varying Flow Rates by Using Time-resolved PIV and POD Technique (Time-resolved PIV와 POD기법을 이용한 유량에 따른 단일노즐 버블링 유동 특성에 관한 연구)

  • Yi, Seung-Jae;Kim, Jong-Wook;Kim, Hyun-Dong;Kim, Kyung-Chun
    • Journal of the Korean Society of Visualization
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    • v.6 no.2
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    • pp.14-19
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    • 2008
  • In this paper, the recirculation flow motion and mixing characteristics driven by air bubble flow in a rectangular water tank is studied. The Time-resolved PIV technique is adopted for the quantitative visualization and analysis. 532 nm Diode CW laser is used for illumination and orange fluorescent particle images are acquired by a PCO 10bit high-speed camera. To obtain clean particle images, 545 nm long pass optical filter and an image intensifier are employed and the flow rates of compressed air is changed from 2 l/min to 4 l/min at 0.5 MPa. The recirculation and mixing flow field is further investigated by the POD analysis technique. It is observed that the large scale counterclockwise rotation and main vortex is generated in the upper half depth from the free surface and one quarter width from the sidewall. When the flow rates are increased, the main vortex core is moved to the side and bottom wall direction.

Characteristics of Bubble-driven Flow by Using Time-resolved PIV and POD Technique (Time-resolved PIV와 POD기법을 이용한 단일노즐 버블링 유동 특성에 관한 연구)

  • Yi, Seung-Jae;Kim, Jong-Wook;Kim, Hyun-Dong;Kim, Kyung-Chun
    • Journal of the Korean Society of Visualization
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    • v.6 no.1
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    • pp.41-46
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    • 2008
  • In this paper, the recirculation flow motion and mixing characteristics driven by air bubble stream in a rectangular water tank is studied. The time-resolved PIV technique is adopted for the quantitative visualization and analysis. 488 nm Ar-ion CW laser is used for illumination and orange fluorescent ($\lambda_{ex}=540nm,\;\lambda_{em}=560nm$) particle images are acquired by a PCO 10bit high-speed CCD camera (1280$\times$1024). To obtain clean particle images, 545 nm long pass optical filter and an image intensifier are employed and the flow rates of compressed air is 3 l/min at 0.5 MPa. The recirculation and mixing flow field is further investigated by time-resolved POD analysis technique. It is observed that the large scale recirculation resulting from the interaction between rising bubble stream and side wall is the most dominant flow structure and there are small scale vortex structures moving along with large scale recirculation flow. It is also verified that the sum of 20 modes of velocity field has about 67.4% of total turbulent energy.