• Applied Microscopy
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• v.25 no.4
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• pp.104-114
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• 1995

The epidermis of scales in the abdominal parts from freshwater- and seawater-adapted guppies(Poecilia reticulatus) were studied respectively by scanning- and transmission- electronmicroscope. In the seawater adapted group, the surface area of pavement cells is increased nearly twice as much as that of freshwater-adapted group, and the pavement cells are relatively flattened. Adaptatoin to seawater causes the increment of the density of glycocalyx in the surface of the microridge of pavement cells, as well as the well developed intercellular junctional complex(desmosome) between neighbouring filament-containing cells. Also, intercellular space between adjacent filament-containing cells is more frequently observed in the epidermis of seawater adapted guppy.

• Applied Microscopy
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• v.39 no.4
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• pp.319-324
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• 2009

Morphology of the gill in Lateolabrax japonicus was investigated after staining the gill, as a result, we found the gill is composed of gill raker, gill arch, gill filament and gill lamellae. The number of gill raker was 7~10 in the upper and 13~18 in the lower. Ultrastructure of the gill in Lateolabrax japonicus were examined by means of the light and transmission electron microscopes. The gill have primary filament and secondary filament (lamellae). The following cells are identified and described: pavement cell, pillar cell, blood cell, mucose cell and chloride cell etc. Simple epithelial layer consists of squamous epithelium contained large nucleus, intracellular organelles etc. and the surface is covered with some of microridges. The lamella pillar structures are characterized by the axial microtubules and lateral membrane interdigitations. The mucous cells were globular in shape, and had almost the mucous granules of same size with various electron density. Chloride cells contain a lot of mitochondria and specifically developed tubular systems.

• International Journal of Highway Engineering
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• v.16 no.4
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• pp.63-74
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• 2014

PURPOSES: This research is to evaluate the mechanical performance of different types of Hot Mix Asphalt (HMA) pavement cells prepared for MN/Road field testing section through an extensive experimental analysis of air voids and simple statistical evaluation tools (i.e. hypothesis test). METHODS: An extensive experimental work was performed to measure air voids in 82 asphalt mixture cores (238 samples in total) obtained from nine different types of road cell located in MN/Road testing field. In order to numerically and quantitatively address the differences in air voids among the different test Cells built in MN/Road, a simple statistical test method (i.e. t-test) with 5% significance was used. RESULTS: Similar trends in air voids content were found among the mixtures including conventional HMA, Reclaimed Asphalt Pavement (RAP) and Warm Mix Asphalt (WMA) combined with taconite aggregate this provides support to the use of RAP and WMA technology in the constructions of asphalt pavement. However, in case of acid modified HMA mixtures, significant differences in air void content were observed between on the wheel path and between wheel path location, which implies negative performances in rutting and thermal cracking resistances. Conclusions : It can be concluded that use of RAP and WMA technology in the construction of conventional asphalt pavement and the use of PPA (Poly Phosphoric Acid) in combinations with SBS (Styrene Butadiene Styrene) in asphalt binder production provide satisfactory performance and, therefore, are highly recommended.

• Geomechanics and Engineering
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• v.11 no.5
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• pp.625-638
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• 2016

This paper presents field study and numerical modeling results for a single-cell low-fill concrete box culvert under a flexible pavement subjected to traffic loading. The culvert in the field test was instrumented with displacement transducers to capture the deformations resulting from different combinations of static and traffic loads. A low-boy truck with a known axle configuration and loads was used to apply seven static load combinations and traffic loads at different speeds. Deflections under the culvert roof were measured during loading. Soil and pavement samples were obtained by drilling operation on the test site. The properties of the soil and pavement layers were determined in the laboratory. A 3-D numerical model of the culvert was developed using a finite difference program FLAC3D. Linear elastic models were used for the pavement layers and soil. The numerical results with the material properties determined in the laboratory were compared with the field test results. The observed deflections in the field test were generally smaller under moving loads than static loads. The maximum deflections measured during the static and traffic loads were 0.6 mm and 0.41 mm respectively. The deflections computed by the numerical method were in good agreement with those observed in the field test. The deflection profiles obtained from the field test and the numerical simulation suggest that the traffic load acted more like a concentrated load distributed over a limited area on the culvert. Elastic models for culverts, pavement layers, and surrounding soil are appropriate for numerical modeling of box culverts under loading for load rating purposes.

• Journal of Korean Society on Water Environment
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• v.36 no.3
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• pp.206-219
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• 2020

As urbanization and impermeable areas have increased, stormwater and non-point pollutants entering the stream have increased. Additionally, in the case of the old town comprising a combined sewer pipe system, there is a problem of stream water pollution caused by the combined sewer overflow. To resolve this problem, many cities globally are pursuing an environmentally friendly low impact development strategy that can infiltrate, evaporate, and store rainwater. This study analyzed the expected effects and efficiency when the LID facility was installed as a measure to improve hydrologic cycle and water quality in the Oncheon stream in Busan. The EPA-SWMM, previously calibrated for hydrological and water quality parameters, was used, and standard parameters of the LID facilities supported by the EPA-SWMM were set. Benchmarking the green infrastructure plan in New York City, USA, has created various installation scenarios for the LID facilities in the Oncheon stream drainage area. The installation and maintenance cost of the LID facility for scenarios were estimated, and the effect of each LID facility was analyzed through a long-term EPA-SWMM simulation. Among the applied LID facilities, the infiltration trench showed the best effect, and the bio-retention cell and permeable pavement system followed. Conversely, in terms of cost-efficiency, the permeable pavement systems showed the best efficiency, followed by the infiltration trenches and bio-retention cells.

• Molecules and Cells
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• v.28 no.1
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• pp.57-65
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• 2009

CDC48 is a member of the AAA ATPase superfamily. Yeast CDC48 and its mammalian homolog p97 are implicated in diverse cellular processes, including mitosis, membrane fusion, and ubiquitin-dependent protein degradation. However, the cellular functions of plant CDC48 proteins are largely unknown. In the present study, we performed virus-induced gene silencing (VIGS) screening and found that silencing of a gene encoding a tobacco CDC48 homolog, NgCDC48, resulted in severe abnormalities in leaf and shoot development in tobacco. Furthermore, transgenic tobacco plants (35S:anti-NgCDC48), in which the NgCDC48 gene was suppressed using the antisense RNA method, exhibited severely aberrant development of both vegetative and reproductive organs, resulting in arrested shoot and leaf growth and sterile flowers. Approximately 57-83% of 35S:anti-NgCDC48 plants failed to develop mature organs and died at early stage of development. Scanning electron microscopy showed that both adaxial and abaxial epidermal pavement cells in antisense transgenic leaves were significantly smaller and more numerous than those in wild type leaves. These results indicate that NgCDC48 is critically involved in cell growth and development of tobacco plants. An in vivo targeting experiment revealed that NgCDC48 resides in the endoplasmic reticulum (ER) in tobacco protoplasts. We consider the tantalizing possibility that CDC48-mediated degradation of an as-yet unidentified protein(s) in the ER might be a critical step for cell growth and expansion in tobacco leaves.

• Journal of the Korean GEO-environmental Society
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• v.14 no.7
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• pp.51-56
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• 2013

Recently, a top record of hourly-based rainfall has been changed annually and flood damages of road have increased. To solve this problem, pavements for drainage were developed and practically constructed but there was no considerations on sub-base. In this research, we proposed standard for distribution of particle size of sub-base to consider strength characteristic and drainage property. We focused to compare coefficients strength and permeability by laboratory tests. Prior to tests, 4 samples were selected under the consideration on the international or domestic design guideline. In the tests, strength characteristics were compared with resilient modulus. Also, permeability characteristics were compared with coefficient of upward and downward permeability. Resilient modulus was determined with MR test using cyclic triaxial testing system. Two permeability tests were carried out. One is variable head permeability test for downward drainage and the other is Rowe Cell test for upward drainage. In the case of Rowe Cell test, middle-sized sampler with 150mm diameter was used for this study. Consequentially, we tried to find the optimum distribution of particle size to satisfy both of strength and permeability characteristics for sub-base.

• Journal of Life Science
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• v.26 no.2
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• pp.164-173
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• 2016

Isotocin (IT), a nonapeptide homolog of oxytocin in mammals, has been suggested to be involved in physiological processes including social behaviors, stress responses, and osmoregulation in teleost fish. To study its structure and function, the gene encoding the IT precursor was cloned from the genomic DNA and brain cDNA of the cinnamon clownfish, Amphiprion melanopus. The IT precursor gene consists of three exons separated by two introns, and encodes an open reading frame of 156 amino acid (aa) residues, comprising a putative signal peptide of 19 aa, a mature IT protein of 9 aa, a proteolytic processing site of 3 aa, and 125 aa of neurophysin. Tissue-specific analysis of the IT precursor transcript indicated its expression in the brain and gonads of A. melanopus. To examine its osmoregulatory effects, the salinity of the seawater (34 ppt) used for rearing A. melanopus was lowered to 15 ppt. Histological analysis of the gills indicated the apparent disappearance of an apical crypt on the surface of the gill lamella of A. melanopus, as pavement cells covered the surface upon acclimation to the lower salinity. The level of Na⁺/K^+-ATPase activity in the gills was increased during the initial stage of acclimation, followed by a decrease to its normal level, suggesting its involvement in osmoregulation and homeostasis. The only slight increase in the level of IT precursor transcript in the A. melanopus brain upon low-salinity acclimation suggested that IT played a minor role, if any, in the process of osmoregulation.