• 대한한방부인과학회지
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• 제31권2호
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• pp.31-48
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• 2018

Objectives: This study was performed to observe the correlations between the results of ABR-2000 and DSOM / 3D-MAC to evaluate the feasibility of ABR-2000 as a oriental medical diagnostic criteria. Methods: We studied 547 women visiting ${\bigcirc}{\bigcirc}$ hospital from December 2012 to June 2015. The subjects were categorized in two groups, 'Hypotonia' and 'Non-Hypotonia' by the result of ABR-2000 and assessed the result of DSOM, 3D-MAC for each group. The differences of pulse wave factors by group also studied. Results: 1. There was no significant difference between two groups about the output frequency of pathogenic factors in DSOM while the result showed the higher correlation in Hypotonia group in terms of the companion tendency of pathogenic factors and syndromes formed by the combination of pathogenic factors. 2. The pulse waves of Hypotonia group were mostly slow, weak, tense and stiff than Non-Hypotonia group. Conclusions: 1. In Hypotonia group, yin deficiency (陰虛) factor was frequently accompanied and consumption (虛損) of various organs based on the yin deficiency (陰虛) was observed. It means chronic and severe condition of exhaustion syndrome (虛勞). 2. The result of 3D-MAC also means pathological feature of yin syndrome (陰 證) and consumption (虛損). Besides, lower scores of Body Surface Area (BSA), body weight, and Body Mass Index (BMI) were associated with body weakness (體瘦), a symptom of exhaustion syndrome (虛勞).

• Tuberculosis and Respiratory Diseases
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• 제43권2호
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• pp.285-290
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• 1996

저자들은 특징적인 임상 증상과 함께 전형적인 흉부 x-선 및 고해상 흉부 전산화 단층 촬영을 보이는 미만성 법세기관지염 환자에서 흉강경을 이용한 폐생검으로 확진하여 erythromycin 소량 투여로 임상적 효과를 얻은 1예를 문헌 고찰과 함께 보고는 바이다.

• Clinical and Experimental Reproductive Medicine
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• 제47권2호
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• pp.108-113
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• 2020

Objective: Endometrial fibrosis, the primary pathological feature of intrauterine adhesion, may lead to disruption of endometrial tissue structure, menstrual abnormalities, infertility, and recurrent pregnancy loss. At present, no ideal therapeutic strategy exists for this fibrotic disease. Eupatilin, a major pharmacologically active flavone from Artemisia, has been previously reported to act as a potent inducer of dedifferentiation of fibrotic tissue in the liver and lung. However, the effects of eupatilin on endometrial fibrosis have not yet been investigated. In this study, we present the first report on the impact of eupatilin treatment on transforming growth factor beta (TGF-β)-induced endometrial fibrosis. Methods: The efficacy of eupatilin on TGF-β-induced endometrial fibrosis was assessed by examining changes in morphology and the expression levels of fibrosis markers using immunofluorescence staining and quantitative real-time reverse-transcription polymerase chain reaction. Results: Eupatilin treatment significantly reduced the fibrotic activity of TGF-β-induced endometrial fibrosis in Ishikawa cells, which displayed more circular shapes and formed more colonies. Additionally, the effects of eupatilin on fibrotic markers including alpha-smooth muscle actin, hypoxia-inducible factor 1 alpha, collagen type I alpha 1 chain, and matrix metalloproteinase-2, were evaluated in TGF-β-induced endometrial fibrosis. The expression of these markers was highly upregulated by TGF-β pretreatment and recovered to the levels of control cells in response to eupatilin treatment. Conclusion: Our findings suggest that suppression of TGF-β-induced signaling by eupatilin might be an effective therapeutic strategy for the treatment of endometrial fibrosis.

• Molecules and Cells
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• 제37권11호
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• pp.767-776
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• 2014

Alzheimer's disease (AD) is clinically characterized with progressive memory loss and cognitive decline. Synaptic dysfunction is an early pathological feature that occurs prior to neurodegeneration and memory dysfunction. Mounting evidence suggests that aggregation of amyloid-${\alpha}$ ($A{\alpha}$) and hyperphosphorylated tau leads to synaptic deficits and neurodegeneration, thereby to memory loss. Among the established genetic risk factors for AD, the ${\varepsilon}4$ allele of apolipoprotein E (APOE) is the strongest genetic risk factor. We and others previously demonstrated that apoE regulates $A{\alpha}$ aggregation and clearance in an isoform-dependent manner. While the effect of apoE on $A{\alpha}$ may explain how apoE isoforms differentially affect AD pathogenesis, there are also other underexplored pathogenic mechanisms. They include differential effects of apoE on cerebral energy metabolism, neuroinflammation, neurovascular function, neurogenesis, and synaptic plasticity. ApoE is a major carrier of cholesterols that are required for neuronal activity and injury repair in the brain. Although there are a few conflicting findings and the underlying mechanism is still unclear, several lines of studies demonstrated that apoE4 leads to synaptic deficits and impairment in long-term potentiation, memory and cognition. In this review, we summarize current understanding of apoE function in the brain, with a particular emphasis on its role in synaptic plasticity and the underlying cellular and molecular mechanisms, involving low-density lipoprotein receptor-related protein 1 (LRP1), syndecan, and LRP8/ApoER2.

• Archives of Plastic Surgery
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• 제37권6호
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• pp.815-818
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• 2010

Purpose: Soft tissue chondroma is a rare benign tumor, found mainly on the palm and sole and grows slowly. Typically, mature hyaline cartilage is the dominant pathological feature. There are reports that assert soft tissue chondromas to be a cause of median nerve entrapment syndrome. However, this is the first case report showing soft tissue chondroma to be a cause of simultaneous median and ulnar neuropathy. Methods: A 62 year-old woman presented with chief complaints of numbness and hypoesthesia of her right palm for 4 to 5 years, and a palpable mass on her right palm that had been increasing in size slowly for 3 years. Physical examination revealed a firm, mobile, non-tender and about $3{\times}3\;cm^2$ sized mass in the center of the right palm. Electromyography showed entrapment neuropathy of the median and ulnar nerve. Ultrasonography showed an approximately $5.7\;cm^2$ mass below the flexor tendon of ring finger. Upon surgical excision, a $3{\times}3\;cm^2$ mass attached to the flexor digitorum profundus of ring finger and redness and hypertrophy of both the median and ulnar nerve were discovered. Mass excision was performed gently and the specimen was referred for histopathologic study. Mass excision resulted in median and ulnar nerve release. Results: The pathology report confirmed the mass to be a soft tissue chondroma with mature hyaline cartilage. The patient exhibited post-operative improvement of her symptoms and did not show any complications. Conclusion: This is the first case report showing soft tissue chondroma to be a cause of simultaneous median and ulnar neuropathy.

• Biomolecules & Therapeutics
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• 제29권2호
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• pp.127-134
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• 2021

Neuroinflammation―a common pathological feature of neurodegenerative disorders such as Alzheimer's disease―is mediated by microglial activation. Thus, inhibiting microglial activation is vital for treating various neurological disorders. 7,3',4'-Trihydroxyisoflavone (THIF)―a secondary metabolite of the soybean compound daidzein―possesses antioxidant and anticancer properties. However, the effects of 7,3',4'-THIF on microglial activation have not been explored. In this study, antineuroinflammatory effects of 7,3',4'-THIF in lipopolysaccharide (LPS)-stimulated BV2 microglial cells were examined. 7,3',4'-THIF significantly suppressed the production of the proinflammatory mediators nitric oxide (NO), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) as well as of the proinflammatory cytokine interleukin-6 (IL-6) in LPS-stimulated BV2 microglial cells. Moreover, 7,3',4'-THIF markedly inhibited reactive oxygen species (ROS) generation. Western blotting revealed that 7,3',4'-THIF diminished LPS-induced phosphorylation of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), glycogen synthase kinase-3β (GSK-3β), and nuclear factor kappa B (NF-κB). Overall, 7,3',4'-THIF exerts antineuroinflammatory effects against LPS-induced microglial activation by suppressing mitogen-activated protein kinase (MAPK) and NF-κB signaling, ultimately reducing proinflammatory responses. Therefore, these antineuroinflammatory effects of 7,3',4'-THIF suggest its potential as a therapeutic agent for neurodegenerative disorders.

• Journal of Gastric Cancer
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• 제4권4호
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• pp.207-212
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• 2004

목적: 고형암이 주위조직으로의 침윤과 타장기로의 전이에는 단백분해효소의 활동이 필요하다. Urokinase type plasminogen activator (uPA)는 serine proteinase의 하나로 세포주의 단백분해와 혈관형성에 중요한 물질로서 고형암의 침윤과 전이에 중요한 역할을 한다. uPA는 유방암, 폐암, 방광암, 위암, 직장결장암, 난소암, 및 연조직 육종에서 중요한 예후지표로 알려져 있다. 본 연구에서 저자들은 위암 조직과 정상 점막에서 uPAR유전자의 발현양상에 대해서 조사하고 기존에 알려진 예후인자와의 연관성을 비교 분석하여 향후 중요한 분자학적 치료 target으로의 역할로서의 의의를 살펴보고자 한다. 대상 및 방법: 1997년 5월 이후 위암으로 진단되어 근치적 혹은 고식적 위장절제술을 시행한 위암 환자 35명을 대상으로 하였다. Northern blot analysis와 RT-PCR을 통해 uPAR mRNA의 발현을 확인하였으며, RNA추출을 위한 재료는 위장절제술 직후 종양조직과 동결절편 생검상 종양세포가 없는 것으로 확인된 부위의 정상 위암 조직을 동일 환자로부터 추출하였다. 이들 환자의 육안적 소견, 조직학적 소견, 생존율 등은 내시경과 수술보고서 병리보고서, 병록지 등을 참고하였다. 결과: uPAR mRNA의 expression과 환자의 예후와의 관계를 평가하기 위해서, gene expression과 이미 확립된 clinicopathologic prognostic와의 관계를 비교하였다. 이러한 factor를 중에서 uPAR mRNA expression은 림프절 전이 여부 (P=0.03), TNM stage (P=0.01)와는 일정한 연관성이 있음을 보였으며, 나이나 성별, 종양크기, histologic type, Lauren classification, Ming classification, serosa invasion, vascular invasion, lymphatic vessel invasion, Neural invasion, omental invasion, macroscopic type와는 상관관계를 보이지 않았다. 결론: 종양 세포에서 uPAR를 평가하는 것은 위암의 예후 뿐만 아니라 질병의 재발을 예측하는데 사용될 수 있을 것으로 생각된다.

• 한국컴퓨터그래픽스학회논문지
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• 제22권3호
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• pp.21-29
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• 2016

본 논문은 명시야 (bright-field) 현미경 영상를 위한 데이터 기반 세포 분할 알고리즘을 제시한다. 제시된 알고리즘은 일반적인 사전 학습 기법과 다르게 동시에 두 개의 사전과 관련된 희소 코드 (sparse code)를 통해 정의된 에너지 함수의 최소화를 진행하게 된다. 두 개의 사전 중 하나는 명시야 영상에 대해 학습된 사전이고 다른 하나는 사람에 의해 수작업으로 세포 분할된 영상에 대해 학습된 것이다. 학습된 두 개의 사전을 세포 분할 될 새로운 입력 영상에 대해 적용하여 이와 관련된 희소 코드를 획득한 후 픽셀 단위의 분할을 진행하게 된다. 효과적인 에너지 최소화를 위해 합성곱 희소 코드 (Convolutional Sparse Coding)와 Alternating Direction of Multiplier Method(ADMM)이 사용되었고 GPU를 사용하여 빠른 분산 연산이 가능하다. 본 연구는 이전에 사용된 가변형 모델 (deformable model)을 이용한 세포 분할 방식과는 다르게 제시된 알고리즘은 세포 분할을 위해 사전 지식이 필요없이 데이터 기반의 학습을 통해서 쉽고 효율적으로 세포 분할을 진행할 수 있다.

• 전자공학회논문지
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• 제53권11호
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• pp.114-122
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• 2016

EIS (Electro interstitial scan, 전기체간스캔법)는 전극을 이용해 미세전류를 인체에 인가하고 그에 따른 전기적 반응을 분석하여 생리적인 정보를 얻는 방법으로, 비침습적이고 간단한 검사가 가능하다는 장점이 있다. 특히 당뇨병 진단을 위한 스크린용으로 적합하다는 연구들이 진행되어 왔으나 대부분 진단 원리에 대한 구체적인 논의가 이루어지지 않았다. 본 연구에서는 EIS 방법이 당뇨병 스크리닝 및 임상에 유용하게 활용될 수 있을지 분석해 보기위해 당뇨병 환자와 정상인을 대상으로 EIS 장비의 원 신호인 전압 변동 데이터를 특정경로에서 측정하였다. 전압 신호의 특징점을 추출하고 두 그룹 사이의 AUC (Area under the curve)를 계산한 결과 7개의 변수들이 60% 이상의 분류 정확도를 보였다. 또한 이 변수들을 k-NN 분류기로 학습한 결과, 왼쪽 손에서의 전압 변동 크기를 기준으로 분석했을 때 분류 정확도를 76.2%까지 높일 수 있었다. EIS 기반의 전압신호 분석법으로 비침습적인 당뇨병 스크리닝의 가능성을 보였다.

• Journal of Chest Surgery
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• 제12권4호
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• pp.324-335
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• 1979

During the period of 4 years from August 1975 to August 1979 one hundred and forty seven cases of lung cancer were seen at Paik Foundation Hospital in Seoul, Korea. Among these 147 cases, 104 patients had primary carcinoma of the lung and the remainder was metastatic carcinoma to the lung. Among these 104 primary carcinoma patients, 73 cases were proven histologically as primary carcinoma of the lung. There were three cases of alveolar cell carcinoma [Table 1 ]. This clinical observation is based on those 73 cases including three case reports of the alveolar cell carcinoma. 1. Peak incidence was observed in the 5th decade of life. Male to female ratio was 2 to 1 [Fig. 1]. 2. Pathological classifications were as follows: epidermoid carcinoma, 24 cases [32.9%]; undifferentiated carcinoma, 20 cases [27.4%]; adenocarcinoma, 15 cases [20.5%]; bronchioloalveolar carcinoma [5.5%] and positive cytology, 10 cases [13.7%] [Fig. 2]. 3. Evidence of inoperability was observed in 55 patients [75% of the 73 cases] [Table 3]. 4. Among those 73 cases, operability was evaluated in 18 patients or 25%. One patient refused operation and 17 patients [23.6%] were explored. In 11 [15%] out of 17 patients, thoracotomies were performed. Six cases were pneumonectomies and 5 cases were lobectomies or bilobectomies [Fig. 3]. 5. First case of alveolar cell carcinoma was a 46 year-old housewife complaining of cough and hemoptysis for one year. The plain chest X-ray and bronchogram showed characteristic pictures as Figures 4 and 5. A pneumonectomy was carried out. Histologically, a beautiful alveolar carcinoma consisted of the characteristic tall columnar epithelial cells, which were lining the alveolar spaces as seen in Figures 6, 7, 8, and 20. 6. In the second case of 41 year old male, predominant clinical feature was single, well defined mass in the right lower lobe [Fig. 10 and 11] on chest X-ray. Bilobectomized specimen showed fragile, soft and hard tissue containing mucoid secretions and focal yellowish necrosis with pigmentation on cut surface [Fig. 12]. Slides showed tumor cells lined up along the alveolar septa with papillary projections [Fig. 13 and 14]. 7. Third case of alveolar cell carcinoma was a 50-year-old housewife with hemoptysis. An outstanding clinical picture was a round to lobulated mass in the right upper lobe [Fig. 16]. She is living now, 2 years and 1 month post-operatively, but has arrived at terminal stage with military nodular disseminations to the contralateral lung [Fig. 19].