• 설비공학논문집
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• 제21권3호
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• pp.149-156
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• 2009

A small-scale thermoelectric cooling system was built in an effort to enhance the performance of the refrigeration system by utilizing the water-cooled jacket which was attached to the hot side of the thermoelectric module. Considered design parameters for the water-cooled jacket were the geometry of the flow passage inside the jacket and the flow rate of cooling water. The higher flow rate of cooling water in the jacket resulted in a better performance of the refrigeration system. The increase in the number of channels for water flow passage inside the cooling jacket also showed significant improvement on the performance of the thermoelectric cooling system such as the cooling capacity and the COP of the refrigeration system.

• Fisheries and Aquatic Sciences
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• 제18권1호
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• pp.81-88
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• 2015

We investigated factors affecting primary cultures of Pacific abalone Haliotis discus hannai ovary-dissociated cells to identify general aspects of their early-phase culture. Ninety-seven cell populations derived from 30 individuals were cultured in different media with varying compositions of medium supplements, and initial attachment, subculture, and survival for ${\geq}10$ weeks were assessed according to medium composition and individual. We also examined the time required for subculture and the rate of cell death according to both culturing period and passage number within 10 weeks. A lack of fetal bovine serum (FBS) and hemolymph significantly inhibited the growth of cultured cells, while we detected no significant effect of medium composition on initial cell attachment. Through data reallocation, with the omission of data from cell populations cultured in FBS-free and hemolymph-free media, we showed that growth inhibition was also affected by individual differences among the abalones used. During the culture, we observed four different types of cell morphology. Moreover, considerable time was required for subculture-18.4 and 19.5 days for first and second subcultures, respectively-and cell death did not occur within 30 days or for passage 0. Our results will provide valuable information for developing universal cell culturing guidelines in abalone species and suggest the feasibility of culturing abalone ovary-dissociated cells.

• Ocean Science Journal
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• 제43권1호
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• pp.61-65
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• 2008

Feeding apparatus, mechanism and passage of ingested prey were described for Nemopilema nomurai (Scyphozoa: Rhizostomeae). N. nomurai medusae without central mouths have developed complicated canal systems connecting the tip of the tentacle and oral arm to the gut cavity. The number of junctions in the canal system increases with the bell diameter. The prey is gathered by paralyzing nematocyst at the tentacles and by adhering cirri at the oral arms and scapulets. They are engulfed into the terminal pore located at the oral arms and scapulets, and entered into the gut cavity via the canal system. The estimated digestion time is 1 hour and 20 min. The diameter of terminal pore is always about 1 mm, implying that they could not eat prey larger than that pore size. On the other hand, ephyrae have central mouths and could swallow prey as large as adults could. Exploitation of the same size of food by adult and ephyra implies that N. nomurai can affect seriously the whole food web, massively ingesting micro- and mesozooplankton and cutting the energy transfer toward the higher level of carnivores.

• 유체기계공업학회:학술대회논문집
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• 유체기계공업학회 2005년도 연구개발 발표회 논문집
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• pp.178-184
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• 2005

The present study investigates the effects of bleed flow on heat/mass transfer and pressure drop in a rotating channel with transverse rib turbulators. The hydraulic diameter ($D_h$) of the square channel is 40.0 mm. The bleed holes are located between the rib turburators on leading surface and the hole diameter (d) is 4.5 mm. The square rib turbulators are installed on both leading and trailing surfaces. The rib-to-rib pitch is 10.0 times of the rib height (e) and the rib height-to-hydraulic diameter ratio ($e/D_h$) is 0.055. The tests were conducted at various rotation numbers (0, 0.2, 0.4), while the Reynolds number and the rate of bleed flow to main flow were fixed at 10,000 and 10%, respectively. The results suggest that the heat/mass transfer characteristics in the internal cooling passage are influenced by rib turbulators, bleed flow and the Cariolis force induced by rotation. For the rotating ribbed passage with bleed flow, the heat/mass transfer on the leading surface is hardly affected by bleed flow, but that on the trailing surface decreases due to the diminution of main flow. The results also show that the friction factor decreases with the bleed flow.

• Plant Biotechnology Reports
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• 제2권2호
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• pp.153-161
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• 2008

The factors influencing somatic embryo maturation, high frequency somatic embryo germination, and plantlet formation were studied in Terminalia chebula Retz. Maturation of somatic embryo were influenced by a number of factors such as in vitro culture passage, concentrations of sucrose, levels of abscisic acid (ABA), basal media and media additive combinations. Maximum frequency of somatic embryo maturation ($57.22{\pm}2.02$), was obtained on MS medium supplemented with 50 g/l sucrose. Different factors such as strengths of MS nutrients, plant growth regulators, media additives and their combinations controlling somatic embryo germination and plantlet formation were studied. High frequency of germination and plantlet formation ($58.80{\pm}1.47$) were achieved by subsequent subculture of mature somatic embryos on MS medium containing 30 g/l sucrose and 0.5 mg/l benzyl-adenine (BA). However, although duration of in vitro passage of the callus tissue was critical, contribution of the combinations of plant growth regulators and media additives showed nugatory effect on somatic embryo maturation and germination as evident from variable responses.

• 대한두개안면성형외과학회지
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• 제15권2호
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• pp.63-69
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• 2014

Background: A number of studies have reported complication after reduction of nasal bone fractures. Among complicated cases, some showed improvement in shape of the nose with passage of time. Therefore, we examined these changes using computed tomography (CT) images taken over intervals. Methods: CT scans of 50 patients with new nasal bone fractures were reviewed, and the images were compared amongst preoperative, immediately postoperative, and one month scans. Changes in nasal bone shape, were evaluated based on the angle of nasal bone arch between the nasal bone and frontal process of maxilla, overall shape of arch, mal-alignment of fracture segments involving bony irregularity or bony displacement. These evaluations were used to separate postoperative outcomes into 5 groups: excellent, good, fair, poor, and very poor. Results: Immediate postoperative nasal shape was excellent in 10 cases, good in 31 cases, fair in 8 cases, and poor results in a single case. Postoperative shape at one month was excellent in 37 cases, good in 12 cases, fair in a single case. Conclusion: The overall shape of nasal bone after fracture reduction tended to improve with passage of time.

• Structural Engineering and Mechanics
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• 제33권4호
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• pp.407-428
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• 2009

The effects of the uniform and spatially varying ground motions on the stochastic response of fluid-structure interaction system during an earthquake are investigated by using the displacement based fluid finite elements in this paper. For this purpose, variable-number-nodes two-dimensional fluid finite elements based on the Lagrangian approach is programmed in FORTRAN language and incorporated into a general-purpose computer program SVEM, which is used for stochastic dynamic analysis of solid systems under spatially varying earthquake ground motion. The spatially varying earthquake ground motion model includes wave-passage, incoherence and site-response effects. The effect of the wave-passage is considered by using various wave velocities. The incoherence effect is examined by considering the Harichandran-Vanmarcke and Luco-Wong coherency models. Homogeneous medium and firm soil types are selected for considering the site-response effect where the foundation supports are constructed. A concrete gravity dam is selected for numerical example. The S16E component recorded at Pacoima dam during the San Fernando Earthquake in 1971 is used as a ground motion. Three different analysis cases are considered for spatially varying ground motion. Displacements, stresses and hydrodynamic pressures occurring on the upstream face of the dam are calculated for each case and compare with those of uniform ground motion. It is concluded that spatially varying earthquake ground motions have important effects on the stochastic response of fluid-structure interaction systems.

• 한국항공우주학회지
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• 제37권6호
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• pp.527-536
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• 2009

캐스케이드 실험장치에 한 개의 익형을 설치하여 캐스케이드 실험이 가능한 장치벽면의 설계를 수행하였다. 장치의 폭은 피치의 160% 넓이이다. 이 경우에 실험장치 내에 다수개의 블레이드를 설치하는 경우에 비하여 소형의 장치라도 실험의 정확성이 향상되는 장점이 있지만, 피치방향으로 주기조건을 맞추기가 어렵다. 본 연구에서는 주기조건이 얻어지도록 벽면의 형상설계를 내부유동장의 결과를 바탕으로 기울기기반과 유전자알고리즘의 방식을 사용하여 벽면을 설계하였다. 이를 위하여 목적함수는 캐스케이드 익형의 표면에서 얻어진 마하수를 적용하였으며, 실험장치의 형상 조정이 가능한 14개의 설계변수를 적용하였다. 유전자알고리즘에 의한 최적화 설계방식이 향상된 결과를 보여주었다.

• The Korean Journal of Physiology and Pharmacology
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• 제10권6호
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• pp.349-354
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• 2006

The aromatic hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates many of the biological and toxicological effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, dioxin) and related chemicals. The application of recombinant reporter plasmid such as the firefly luciferase gene has proven to be a very effective method to detect these chemicals. The bioassay system, CALUX, is sensitive in directly detecting AhR-agonists from a variety of environmental and biologic materials. However, responses of the AhR-dependent bioassays are dependent on the cell types used. Thus, we developed a sensitive bioassay using the recombinant mouse hepatoma cell (Hepa1c1c7) for the determination of dioxins. The recombinant cell line was stably transfected with firefly luciferase reporter gene (pGudLuc1.1). The transfected cells showed the highest induction of luciferase activity at 4.5 hr and a decrease beyond this time point. The system showed the highest sensitivity of detection ever reported. Upon TCDD exposure cells showed 2 fold increase at 10 pM and 7 fold increase at 100 pM, respectively. The passage number after the transfection played an important role in the sensitivity. The increase of passage number tended to increase the sensitivity of the cells up to 15. The media without phenol red showed a higher induction rate than with phenol red, suggesting the preferable use of phenol red-free media for the bioassay. Since each of the assays has unique characteristics that make them suitable for some screening applications and not others, development of sensitive bioanalytical methods based on a variety of cellular systems in a key to the successful determination of dioxins. The bioassay system developed in this study will contribute to further development of successful screening the AhR agonists among the environmental mixture. In addition, the rapid and sensitive nature of this cellular system can be applied as a valuable tool to screen the dioxin-like moieties among the prodrugs at the initial stage, thereby expediting the new drug discovery.

• 한국식품조리과학회지
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• 제12권2호
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• pp.129-137
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• 1996

The purpose of this study was that to estimate the microbial and sensory quality by the method and time of holding in the step of holding of Cow's Knee Broth, Stir-Fry Pork and Cooked Cuttlefish that were provide at the industry foodservice establishment, then to suggest safer feeding by the reasonable holding method. The results are as follows: Cow's Knee Broth: holding at room temperature was shown that the number of mi- crobiology by passage of the holding time was higer than the case of holding at steam table, but it was lower after reheating it. As a result of sensory test, it was exellent to holding at steam table and to reheat after holding room temperature within four hours and six hours each. Stir-Fry Pork: as a result of microbial analysis, in the case of holding at the steam table microbes increased safer two hours, but after four and six hours they little reduced. In the case of holding at room temperature the number of microbes increased according to the passage of time. As a result of sensory analysis, its taste of room temperature holding was fine until four and six hours each. Cooked Cuttlefish: as a microbial analysis, it was shown that the amount of total plate counts of room temperature holding ten times as high as the latter after six hours. As the result of sensory analysis, the cold holding was better until six hours and the room temperature holding was fine just until two hours. As a result of food poisoning bacteria, it was negative in every test in sample against Salmonella, Staphylococcus aureus, Vibrio parahaemolyticus.