• Title/Summary/Keyword: Passage number

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Comparison on the Time series of Housing Viewpoint of University Student (대학생 주거관의 시계열적 비교)

Comparison of Neural Cell Differentiation of Human Adipose Mesenchymal Stem Cells Derived from Young and Old Ages (연령별 지방 중간엽 유래 줄기세포의 신경세포로의 분화 능력 비교)

  • Jo, Jung-Youn;Kang, Sung-Keun;Choi, In-Su;Ra, Jeong-Chan
    • Development and Reproduction
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    • v.13 no.4
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    • pp.227-237
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    • 2009
  • Recently, adipose mesenchymal stem cells (AdMSC) that are similar to bone marrow MSC and blood derived MSC are thought to be another source for stem cell therapy. However, the diseases that can be applied for stem cells therapy are age-dependent degenerative diseases. Accordingly, the present study investigated the growth and differentiation potential to neural cells of human AdMSC (hAdMSC) obtained from aged thirty, forty and fifty. The growth of cells and cell viability were measured by passage and neural differentiation of hAdMSC was induced in neural differentiation condition for 10 days. Our results demonstrated that cell number, viability and morphology were not different from hAdMSC by age and passage. Immunofluorescence analysis of neural cell marker (TuJ1, NSE, Sox2, GFAP or MAP2) demonstrated no significant differences in neural cell differentiation by age and passage. As the number of passage was increased, the mRNA level of MAP2 and Sox2 was decreased in hAdMSC from age of 50 compared to hAdMSC from age of 30. In conclusion, the present study demonstrated that ability of neural cell differentiation of hAdMSC was maintained with ages, suggesting that autologous stem cells from aged people can be applied for stem cell therapy with age-dependent neural disease with the same stem cell quality and ability as stem cell derived from young age.

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Development of Somatic Cell Nuclear Transfer Embryos Following Donor Cell Type and Cell Treatment in Cattle (Donor 세포의 종류 및 세포처리에 따른 소 체세포 핵이식란의 체외발육)

  • 손준규;박정준;박춘근;양부근;김정익;정희태
    • Reproductive and Developmental Biology
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    • v.28 no.1
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    • pp.1-6
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    • 2004
  • This study was conducted to investigate the effects of donor cell type, individual, passage number and trypsinization time on the in vitro development of bovine somatic cell nuclear transfer embryos. Three cell types (skin, muscle and cumulus cells) and cells from 3 individuals were used for nuclear transfer. Cell were passaged by 5, 15 or 30 times, and cell were trypsinized for 1 or 3 min before injection. Nuclear transfer were performed by conventional fusion method. Development rates to the blastocyst stage were not significantly different among three cell types (16.5∼23.9%) and individuals (16.4∼19.5%). Blastocyst formation rate of cloned embryos reconstituted with cells at passage 30 (5.8%) was significantly lower than those of embryos reconstituted with 5- and 15-passaged cells (25.3 and 23.5%, respectively, P<0.05). The rate of embryos developed to the blastocyst stage was higher in embryos reconstituted with cells trypsinized for 1 min (30.7%) compared to embryos reconstituted with cells trypsinized for 3 min (P<0.05). The result of the present study indicates that different donor cell types and individuals used in this study did not affect the development of cloned bovine embryos. However, passage number and trypsinization time of donor cells affect the in vitro development of cloned bovine embryos.

A Numerical Study on Heat Transfer and Flow Characteristics of a Finned Downhole Coaxial Heat Exchanger (외부유로 내벽에 설치된 핀 형상에 따른 이중관 열교환기의 열전달 및 유동에 대한 수치해석적 연구)

  • Park, Chun Dong;Lee, Dong Hyun;Park, Byung-Sik;Choi, Jaejoon
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.41 no.2
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    • pp.79-86
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    • 2017
  • In this study, the flow and heat transfer characteristics of the finned annular passage were investigated numerically. The annular passage simulates co-axial geothermal heat exchanger, and fins are installed on its inner wall to reduce heat loss from the production passage (annulus) to injection passage (inner pipe). A commercial CFD program, Ansys Fluent, was used with SST $k-{\omega}$ turbulence model. The effects of the geometric parameters of the fin on the inner tube were analyzed under the periodic boundary condition. The result indicated that most parameters had a tendency to increase with an increase in the height and angle of the fin. However, it was confirmed that the Nusselt number of the inner tube on the coaxial 15, 5, 0.3 was lower than that of the smooth tube. Additionally, the Nusselt number of the inner tube exhibited a tendency of decreasing with a decrease in the spacing in Coaxial 15, $S_f$, 0.3.

A Numerical Study on Aerodynamic Performance by the Blade Mach Number of the Centrifugal Compressor (원심 압축기의 임펠러 마하수에 따른 공력성능 특성에 관한 수치해석적 연구)

  • Heo, Won-Seok;Kang, Shin-Hyoung
    • The KSFM Journal of Fluid Machinery
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    • v.18 no.4
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    • pp.56-61
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    • 2015
  • It is important requirement to properly evaluate the aerodynamic performance and characteristics during preliminary design of a centrifugal compressor. In this study the centrifugal compressor was calculated for variations of mass flow and blade Mach number by means of single passage steady state. A lot of quantitative performance values were obtained and through the obtained values the aerodynamic performance characteristics of designed impeller and vaned diffuser were investigated. The results were classified by blade Mach number to analyze characteristics and the aerodynamic performance was examined at choke of impeller, diffuser and separation of diffuser.

A Study on the Effects of Fumigation on Excavated Costumes (출토복식유물에 대한 훈증소독의 영향에 관한 연구)

  • 채옥자;박성실;안춘순
    • Journal of the Korean Society of Clothing and Textiles
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    • v.28 no.5
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    • pp.668-677
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    • 2004
  • This study examines the effects and influences of fumigation on the antimicrobial treatment of excavated dresses in two different museum settings using chemical composite of Methyl Bromide and Ethylene Oxide. The microbial test indicated that the prewashed samples contained Micrococcus, Bacillus, Staphylococcus, and some unidentified fungi and that the total number of microbes decreased after washing. While it was observed that most germs were removed immediately after fumigation, Bacillus was still found and its number even increased. After 5 months, the number of microbes found in Museum A was smaller than Museum B, which shows that Museum A equipped with better environment control facility was superior to Museum. B in terms of exhibition and preservation of dresses against organic damages. In addition, the result indicates that fumigation is an effective measure against the growth and reproduction of microorganisms, considering that the number of microorganisms decreased after fumigation. The color of test sample showed immediate change after fumigation but color difference became stabilized and decreased with the passage of time. The test samples from both museums showed similar patterns.

Sexing and Cell Cycle Induction Hanwoo Fetal Fibroblast Cells (한우 섬유아세포의 성 판별 및 세포주기 유도 분석)

  • 김현주;강회성;최화식;이성호;박창식;진동일
    • Korean Journal of Animal Reproduction
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    • v.27 no.1
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    • pp.53-59
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    • 2003
  • For somatic cell nuclear transfer in Hanwoo, fetal fibroblast cell lines were established from 35, 50, 70 and 90-day fetuses of Korean native cattle. The sex of these fetal fibroblast cells were analyzed by PCR using Y-specific primers and confirmed that two cell lines were female and the other two cell lines were male. Karyotyping of these cell lines indicates that the chromosome numbers of fetal fibroblast cells were not affected by passage number and more than 80% of fetal fibroblast cells have normal chromosome number. To evaluate Go stage in cell cycle of fetal fibroblast cells, Western blotting was performed to detect the expression level of PCNA which is known to be expressed in all cell cycle stages except G$_{0}$ stage. Following serum starvation or confluent culture for 7 days, fetal fibroblast cells were effectively reached to G$_{0}$ stage. The cell cycle was resumed after culture of these Go stage-fetal fibroblast cells with normal medium. These results indicates that fetal fibroblast cells originated from Hanwoo were successfully isolated and culture system and induction of cell cycle of these cells were established for somatic cell nuclear transfer in Hanwoo.woo.

Choked Surge in a Cavitating Turbopump Inducer

  • Watanabe, Toshifumi;Kang, Dong-Hyuk;Cervone, Angelo;Kawata, Yutaka;Tsujimoto, Yoshinobu
    • International Journal of Fluid Machinery and Systems
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    • v.1 no.1
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    • pp.64-75
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    • 2008
  • During an experimental investigation on a 3-bladed and a 4-bladed axial inducer, a severe surge instability was observed in a range of cavitation number where the blade passage is choked and the inducer head is decreased from noncavitating value. The surge was stronger for the 4-bladed inducer as compared with a 3-bladed inducer with the same inlet and outlet blade angles. For the 4-bladed inducer, the head decreases suddenly as the cavitation number is decreased. The surge was observed after the sudden drop of head. This head drop was found to be associated with a rapid extension of tip cavity into the blade passage. The cause of surge is attributed to the decrease of the negative slope of the head-flow rate performance curve due to choke. Assuming that the difference between the 3 and 4-bladed inducers is caused by the difference of the blockage effects of the blade, a test was carried out by thickening the blades of the 3-bladed inducer. However, opposite to the expectations, the head drop became smoother and the instability disappeared on the thickened blade inducer. Examination of the pressure distribution on both inducers could not explain the difference. It was pointed out that two-dimensional cavitating flow analyses predict smaller breakdown cavitation number at higher flow rates, if the incidence angle is smaller than half of the blade angle. This causes the positive slope of the performance curve and suggests that the choked surge as observed in the present study might occur in more general cases.

Changes in Microstructures of AA3103 sheets deformed by ECAR (ECAR 가공한 AA3103 판재의 미세조직 변화)

  • Lee J. P.;Huh M. Y.;Chung Y. H.;Park J. W.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2004.05a
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    • pp.228-230
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    • 2004
  • Samples of the aluminum alloy 3103 sheets were repeatedly deformed by ECAR up to twelve passes. Shear textures developed after the first passage of ECAR. However, the intensity of shear texture components decreased with increasing number of ECAR passages. Observations by TEM and EBSD revealed that the degree of misorientations within the deformed grains increased with increasing number of ECAR passes. Changes in textures and microstructures were hardly observed during recrystallization anneal. Upon subsequent annealing, the samples deformed by a large number of ECAR passes displayed a continuous grain growth. A higher deformation by ECAR resulted in a slower softening, which reflects the stability of ultra-fine grains against the grain growth.

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Solar Cell Design for Large Area Multi Busbar Module Power Loss Reduction (대면적 Multi busbar 모듈 전력 손실 저감을 위한 태양전지 설계)

  • Juhwi Kim;Jaehyeong Lee
    • Current Photovoltaic Research
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    • v.11 no.1
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    • pp.34-37
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    • 2023
  • Solar energy had become the main energy industry of renewable energy along with hydroelectric power generation. One of the technologies that contributed to the popularization of photovoltaic power and the decrease in the unit price of photovoltaic modules was the large-area solar cell. However, as the area increased, the light receiving area increased and the current value increased accordingly. Since power loss occurs when the current value was large, the number of busbar was increased to increase the current collection rate, and a technology to lower the current value through half-cutting was developed. The bus bar of the solar cell served as a passage through which the generated current was transmitted. This was because when the number of busbar decreases, the moving distance of electrons increased, so the amount of power generation decreases and when it increases, shadows occured. An important aspect of the electrode design was the optimal balance of these busbars and number of fingers. Therefore, in this study, the characteristics of the solar cell according to the number of front bus bars of the large-area solar cell were simulated using Griddler 2,5 pro. After selecting the number of busbar with the best characteristics, the difference was compared by varying the number of fingers and a better direction for the number of cutting was presented.