• 제목/요약/키워드: Parentage verification

검색결과 25건 처리시간 0.032초

Microsatellite DNA Typing Using 16 Markers for Parentage Verification of the Korean Native Horse

  • Cho, G.J.;Cho, B.W.
    • Asian-Australasian Journal of Animal Sciences
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    • 제17권6호
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    • pp.750-754
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    • 2004
  • This study was performed for parentage verification of the Korean native horse (KNH). 103 random KNH samples (including 19 foals for parentage testing) were genotyped by using 16 microsatellite markers. The number of alleles per locus varied from 5 to 13 with an average value of 8.56 in the KNH. The observed heterozygosity and the expected heterozygosity ranged 0.398-0.893 (the average value was 0.683) and 0.368-0.871 (the average value was 0.727) in the KNH, respectively. The PIC value and the exclusion probability ranged 0.347-0.853 (the average value was 0.692) and 0.208-0.736, respectively, and the total exclusion probability of 16 microsatellite loci was 0.9999. Of the 16 markers, AHT4, AHT5, ASB2, ASB17, HMS2, HMS3, HTG10, LEX33, TKY321 and VHL20 loci have a relatively high PIC value (>0.7) in the KNH. Of the 19 foals, 5 foals were disqualified by an incompatibility of 4-7 markers according to a Mendelian fashion in the present DNA typing for parentage testing. These results present basic information for developing a system for parentage verification and individual identification in the KNH.

Microsatellite DNA형 분석을 이용한 더러브렛 말의 친자감정 (Parentage Testing for Thoroughbred Horse by Microsatellite DNA Typing)

  • 조길재
    • Journal of Animal Science and Technology
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    • 제46권2호
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    • pp.129-136
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    • 2004
  • The objective of present study was to ascertain parentage of Thoroughbred(TB) horses in Korea. A total of 2,029 TB horse samples including 993 foal samples for parentage testing were genotyped for nine international minimum standard markers(AHT4, 5, ASB2, HMS3, 6, 7, HTG4, 10, and VHL20). This method consisted of multiplexing PCR procedure, and showed reasonable amplification of all PCR products. Genotyping was performed with an ABI 310 genetic analyzer. The number of alleles per locus varied from 5 to 11 with a mean value of 7.33 in TB. Expected heterozygosity was ranged from 0.544 to 0.837(mean 0.709) and the total exclusion probability of 9 microsatellites loci was 0.9978. Of the 9 markers, ASB2, HMS7 and HTG10 loci have relatively high PIC value(>0.7). All of the 993 foals were qualified by compatibility according to Mendelian fashion in the present DNA typing for parentage testing. These results suggest that the present DNA typing has high potential for parentage verification of TB horses.

Validation of 17 Microsatellite Markers for Parentage Verification and Identity Test in Chinese Holstein Cattle

  • Zhang, Yi;Wang, Yachun;Sun, Dongxiao;Yu, Ying;Zhang, Yuan
    • Asian-Australasian Journal of Animal Sciences
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    • 제23권4호
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    • pp.425-429
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    • 2010
  • To develop an efficient DNA typing system for Chinese Holstein cattle, 17 microsatellites, which were amplified in four fluorescent multiplex reactions and genotyped by two capillary electrophoresis injections, were evaluated for parentage verification and identity test. These markers were highly polymorphic with a mean of 8.35 alleles per locus and an average expected heterozygosity of 0.711 in 371 individuals. Parentage exclusion probability with only one sampled parent was approximately 0.999. Parentage exclusion probability when another parent' genotype was known was over 0.99999. Overall probability of identity, i.e. the probability that two animals share a common genotype by chance, was $1.52{\times}10^{-16}$. In a test case of parentage assignment, the 17 loci assigned 31 out of 33 cows to the pedigree sires with 95% confidence, while 2 cows were excluded from the paternity relationship with candidate sires. The results demonstrated the high efficacy of the 17 markers in parentage analysis and individual identification for Chinese Holstein cattle.

Miniature 말의 성(sex) 결정과 친자감정 (Sex Determination and Parentage Testing In Miniature Horses)

  • 조길재;조병욱
    • 생명과학회지
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    • 제15권1호
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    • pp.45-48
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    • 2005
  • 서울대공원에서 사육중인 Miniature horse 10두의 혈통정립을 목적으로 PCR에 의한 성별 판정 및 16개 microsatellite marker를 사용하여 친자감정을 실시하였다. 성별 판정에서 430 bp의 SRY band가 관찰된 7두는 숫말로 판정되었고, 친자감정에서는 멘델의 유전양식에 부합된 3두가 친자관계가 확인되었다. 향후 Miniature horse의 혈통보존 및 관리에 유용한 자료가 될 것으로 사료된다.

Microsatellite DNA형에 의한 개의 친자감정예 (A Case of Parentage Testing in Dog by Microsatellite DNA Typing)

  • 조길재;조병욱;이길왕;김선구;김용균
    • 생명과학회지
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    • 제13권4호
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    • pp.416-420
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    • 2003
  • Microsatellite DNA형에 의한 개의 친자감정을 실시한 결과 다음과 같은 결론을 얻었다. Labrador Retriever Pup I과 Pup II는 12개 marker 모두에서 멘델의 유전법칙에 따라 친자관계가 성립되었으나 풍산개인 Pup III은 PEZ1 (106bp/118bp), PEZ10 (276bp/300bp), FHC2010 (228bp/232bp) 등 3개 marker에서 유전법칙에 어긋나 친자관계가 성립되지 않았다.

Evaluation of recent changes in genetic variability in Thoroughbred horses based on microsatellite markers parentage panel in Korea

  • Park, Chul Song;Lee, Sun Young;Cho, Gil Jae
    • Animal Bioscience
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    • 제35권4호
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    • pp.527-532
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    • 2022
  • Objective: In this study, we aimed to investigate the recent changes such as allele frequencies and total probability of exclusion (PE) in Thoroughbred horses in Korea using short tandem repeat (STR) parentage panels between 2006 and 2016. Methods: The genotype was provided for 5,988 horse samples with 15 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG10, LEX3 and VHL20). Results: In our study, the observed number of alleles per locus ranged from 3 (HMS1) to 9 (ASB17) in 2006 and 4 (HMS1) to 9 (ASB2) in 2016, with a mean value of 6.28 and 6.40, respectively. Of the 15 markers, HMS2, HTG4, and CA425 loci had relatively low polymorphism information content (<0.5000) in the Thoroughbred population. Mean levels of genetic variation in 2006 and 2016 were observed heterozygosity (HO) = 0.708, and expected heterozygosity (HE) = 0.685, as well as and HO = 0.699 and HE = 0.682, respectively. The PE was calculated for each group based on the allele frequencies of 14 or 15 STRs. The 2006 survey analyzed that PE was 0.9998, but it increased to 0.9999 in 2016 after the HMS2 marker was added in 2011. The current STR panel is still a powerful tool for parentage verification that contributes to the maintenance of integrity in the Thoroughbred population. Conclusion: The current STR panel is still a powerful tool for parentage verification that contributes to the maintenance of integrity in the Thoroughbred horses. However, continuous monitoring genetic variability is necessary.

재래흑염소 개체식별과 친자확인을 위한 Microsatellite Marker Set 개발 (Development of a Microsatellite Marker Set for the Individual Identification and Parentage Verification of Korean Native Black Goats)

  • 이상훈;강호찬;이성수;이진욱;김은호;명철현;김관우;임현태
    • 생명과학회지
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    • 제30권10호
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    • pp.912-918
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    • 2020
  • 본 연구는 재래흑염소와 교잡종 염소 총 304두를 대상으로 Microsatellite (MS) marker의 대립유전자형 분석을 통해 염소의 개체식별과 친자확인을 목적으로 실시하였다. 각 MS marker 별 대립유전자형의 다형성을 토대로 11종의 MS marker를 선발하였다. 선발된 MS marker를 사용할 경우 동일한 유전자형을 가진 개체가 출현할 확률이 무작위, 반형매 교배집단에서 각각 5.58×10-10, 1.15×10-7으로 분석되었다. 또한 친자감정 확률은 부모의 정보가 있을 경우 0.999996, 부모의 정보가 없을 경우 0.999833으로 분석되어 국내에서 사육하고 있는 염소들의 개체식별 및 친자확인이 가능할 것으로 사료된다. 또한 국내 재래흑염소 4 계통과 교잡종 염소들 간의 혈연관계 분석을 통해 국내 재래흑염소의 유전적 특성을 확인하였다. 본 연구의 결과는 염소의 개량 기반 구축에 필요한 개체관리와 친자감별 및 향후 염소고기의 생산 이력 구축에 유용하게 활용 할 수 있을 것으로 판단된다.

Microsatellite DNA형에 의한 더러브렛 말의 친자감정예 (A case of parentage testing in the Thoroughbred horse by microsatellite DNA typing)

  • 조길재;양영진;김봉환
    • 대한수의학회지
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    • 제43권1호
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    • pp.25-29
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    • 2003
  • This study was carried out to investigate a usefulness of the microsatellite DNA markers for parentage verification of Thoroughbred (TB) horses. 9 TB horses samples were genotyped for nine international minimum standard markers (AHT4, 5, ASB2, HMS3, 6, 7, HTG4, 10, and VHL20), and the additional panel of four markers, ASB17, CA425, LEX33, and TKY321. This methods consisted of multiplexing PCR procedures, and it showed reasonable amplification of all PCR products. Genotyping was performed with an ABI 310 genetic analyzer. Foal I was excluded according to principles of Mendelian genetics in AHT4 (H/K), ASB2 (Q/Q), HMS3 (I/P), HTG4 (M/O), HTG1O (K/R), VHL20 (M/P), ASB17 (F/N), LEX33 (M/O), and TKY321 (G/I) markets. Foal II was excluded with markers AHT5 (K/M), ASB2 (M/N), HMS7 (N/N), HTG1O (K/K), VHL20 (I/I), ASB17 (F/F) and TKY321 (G/I). Foal III was excluded with markers AHT4 (O/O), AHT5 (K/K), ASB2 (M/R), HMS6 (M/P), HMS7 (O/O), HTG10 (R/S), VHL20 (L/M), and ASB17 (N/O). These results suggest that the present DNA typing is so useful for parentage verification of TB horses.

Accuracy of Imputation of Microsatellite Markers from BovineSNP50 and BovineHD BeadChip in Hanwoo Population of Korea

  • Sharma, Aditi;Park, Jong-Eun;Park, Byungho;Park, Mi-Na;Roh, Seung-Hee;Jung, Woo-Young;Lee, Seung-Hwan;Chai, Han-Ha;Chang, Gul-Won;Cho, Yong-Min;Lim, Dajeong
    • Genomics & Informatics
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    • 제16권1호
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    • pp.10-13
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    • 2018
  • Until now microsatellite (MS) have been a popular choice of markers for parentage verification. Recently many countries have moved or are in process of moving from MS markers to single nucleotide polymorphism (SNP) markers for parentage testing. FAO-ISAG has also come up with a panel of 200 SNPs to replace the use of MS markers in parentage verification. However, in many countries most of the animals were genotyped by MS markers till now and the sudden shift to SNP markers will render the data of those animals useless. As National Institute of Animal Science in South Korea plans to move from standard ISAG recommended MS markers to SNPs, it faces the dilemma of exclusion of old animals that were genotyped by MS markers. Thus to facilitate this shift from MS to SNPs, such that the existing animals with MS data could still be used for parentage verification, this study was performed. In the current study we performed imputation of MS markers from the SNPs in the 500-kb region of the MS marker on either side. This method will provide an easy option for the labs to combine the data from the old and the current set of animals. It will be a cost efficient replacement of genotyping with the additional markers. We used 1,480 Hanwoo animals with both the MS data and SNP data to impute in the validation animals. We also compared the imputation accuracy between BovineSNP50 and BovineHD BeadChip. In our study the genotype concordance of 40% and 43% was observed in the BovineSNP50 and BovineHD BeadChip respectively.

소의 친자감정을 위한 Microsatellite markers의 유전적 다양성 분석 (Analysis of genetic diversity for cattle parentage testing using microsatellite markers)

  • 조길재;양영진;이길왕
    • 대한수의학회지
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    • 제44권2호
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    • pp.287-292
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    • 2004
  • The objective of present study was to ascertain genetic diversity for cattle parentage testing. A total of 59 random cattle samples(29 Korean native cattle and 30 dairy cows) were genotyped by using 11 microsatellite loci(BM1824, BM2113, ETH10, ETH225, EH3, INRA23, SPS115, TGLA122, TGLA227, TGLA53, and TGLA126). This method consisted of multiplexing PCR procedure and showed reasonable amplification of all PCR products. Genotyping was performed with an ABI 310 genetic analyzer. The number of alleles per locus varied from 5 to 11 with a mean value of 6.73 in the Korean native cattle(KNC), 4 to 9 with a mean of 5.91 in dairy cows(DC). Expected heterozygosity was ranged 0.534~0.855(mean 0.732), 0.370~0.866(mean 0.692) in the KNC and DC, respectively. PIC value was ranged 0.485~0.821(mean 0.684), 0.336~0.834(mean 0.640) in the KNC and DC, respectively. Of the 11 markers, 7 markers(ETH10, EH3, INRA23, SPS115, TGLA122, TGLA227, TGLA53) and 3 markers(INRA23, TGLA227, TGLA53) have relatively high PIC value (>0.7) in the KNC and DC, respectively. The total exclusion probability of 11 microsatellite loci was 0.9997 and 0.9991 in the KNC and DC, respectively. These results present basic information for developing a system for parentage verification and individual identification in the KNC and DC.