Browse > Article
http://dx.doi.org/10.5352/JLS.2003.13.4.416

A Case of Parentage Testing in Dog by Microsatellite DNA Typing  

조길재 (한국마사회 유전자검사실)
조병욱 (밀양대학교 동물자원학과)
이길왕 (밀양대학교 동물자원학과)
김선구 (밀양대학교 동물자원학과)
김용균 (밀양대학교 생물공학과)
Publication Information
Journal of Life Science / v.13, no.4, 2003 , pp. 416-420 More about this Journal
Abstract
We performed this study to determine the parentage verification of putative dogs. A total of 7 samples (4 Labrador Retriever dog and 3 Poongsan dog) were genotyped by using 12 international markers (PEZ1, PEZ5, PEZ8, PEZ10, PEZ11, PEZ12, PEZ17, PEZ20, PEZ21, FHC2010, FHC2054, FHC2079). This methods consisted of multiplexing PCR procedures, and it showed reasonable amplification of all PCR products. Genotyping was performed with an ABI 310 genetic analyzer. Labrador Retriever Pup I and Pup II were included according to principles of Mendelian genetics in all loci, while Poongsan Pup III was excluded with markers PEZ1 (106/118), PEZ10 (276/300), and FHC2010 (228/232). These results suggest that the present DNA typing is so useful for parentage verification of these two breeds.
Keywords
dog; microsatellite; parentage verification;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kim, K. S. and C. B. Choi. 2002. Genetic structure of Korean native pig using microsatellite markers. Korean J. Genetics 24, 1-7.   과학기술학회마을
2 Jeffreys A. J., V. Wilson and S. L. Thein. 1985. Hypervariable minisatellite regions in human DNA. Nature 314, 67-73.   DOI   ScienceOn
3 Glowatzki-Mullis, M. L., C. Gaillard, G. Wigger and R. Feies. 1995. Microsatellite-based parentage control in cattle. Animal Genetics 26, 7-12.   DOI   ScienceOn
4 Litt, M. and J. A. Luty. 1989. A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. American J. Human Genetics 44, 397-401.
5 Han, S. K., H. D. Byun and E. Y. Chung. 2000. Genetic diversity analysis and parentage control in Korean native cattle using Microsatellite. Proc. 27th ISAG Conference on Animal Genetics. pp. 88.
6 Cho, G. J., Y. J. Yang, H. S. Kang and B. W. Cho. 2002. Genetic diversity and validation of microsatellite markers for Jeju native horse parentage testing. Korean J. Genetics 24, 359-365.   과학기술학회마을
7 Tozaki, T., H. Kakoi, S. Mashima, K. I. Hirota, T. Hasegawa, N. Ishida, N. Miura, N. H. Choi-Miura and M. Tomita. 2001. Population study and validation of paternity testing for thoroughbred horses by 15 microsatellite loci. J. Veterinary Medicine Science 63, 1191-1197.   DOI   ScienceOn
8 Cho, G. J. 2002. Microsatellite DNA polymorphism of thoroughbred horses in Korea. Korean J. Genetics 24, 177-182.   과학기술학회마을
9 Halverson, J. L. and J. W. Edwards. 2000. Microsatellite polymorphism in dog breeds-the AKC Parent Club study. Proc. 27th ISAG Conference on Animal Genetics. pp. 19.
10 Fredholm, M. and A. K. Wintero. 1995. Efficient resolution of parentage in dogs by amplification of microsatellites. Animal Genetics 27, 19-23.