To construct useful yeast strain for bioethanol production, we improved yeast harboring various phenotypes by using yeast protoplast fusion method. In this study, S. cerevisiae BYK-F11 strain which have ethanol tolerance, thermotolerance and ${\beta}-glucanase$ activity and P. $stipitis{\Delta}ura$ strain which has xylose metabolism pathway were fused by genome shuffling. P. $stipitis{\Delta}ura$ strain was constructed for protoplast fusion by URA3 gene disruption, resulting in uracil auxotroph. By protoplast fusion, several fused cells were selected and BYKPS-F8 strain (fused cell) showing both karyotypes from two parent strains (S. cerevisiae BYK-F11 and P. $stipitis{\Delta}ura$ strain) among 22 fused cells was finally selected. Sequentially, various phenotypes such as ${\beta}-glucanase$ activity, xylose utility, ethanol tolerance, thermotolerance and ethanol productivity were analyzed. The BYKPS-F8 strain obtained ${\beta}-glucanase$ activity from BYK-F11 strain and 1.2 fold increased xylose utility from P. $stipitis{\Delta}ura$ strain. Also, the BYKPS-F8 strain showed thermotolerance at $40^{\circ}C$ and increased ethanol tolerance in medium containing 8% ethanol. In this fused cell, 7.5 g/l ethanol from 20 g/l xylose was produced and the multiple phenotypes were stably remained during long term cultivation (260 hr). It was proved that novel biological system (yeast strains) is easily and efficiently bred by protoplast fusion among yeasts having different genus.
Asia-Pacific Journal of Business Venturing and Entrepreneurship
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v.13
no.6
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pp.27-38
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2018
The purpose of this study is to find out common investment decision factors for CVC's invested technology-based startups and analyze them. We examined 17 CVCs that invested in technology startups for three years and six months from 2015 to June 2018. As a result, the final 9 CVCs that can be used in this study were confirmed and 188 companies were analyzed. This study was conducted as a case study to propose and demonstrate CVC investment objectives and investment decision factors analysis model. The results of this study are as follows. First, CVC focused on strengthening investment. Second, In 2015, Invested in an average of 19 months of technology-based startups. In recent years, we invested in 36 months of proven technology-based startups. Thirdly, ICT service was the main business type of the invested startups. Fourth, the investors were concentrated on the stage of Series A~B. It is observed that CVC investment determinants have a significant impact on product or service and parent company relations. In addition, it was found that factors such as innovation, business planning competency, enterprising, strategic competency, leadership, and opportunity recognition competency were influential factors for the startups of invested companies and it was found that these factors are important for CVC investment decision. Understanding of CVC investment determinants presented in this study is based on the establishment of the investment process of the investee, entrepreneurship and management education program. The results of this study can be applied to the selection of excellent startups, entrepreneurship education programs, mentoring, development of coaching guidelines, and establishment of investment process of other investment institutions when investing in CVC.
This study was conducted to identify a relationship between Helicopter Parenting, Critical thinking disposition, and Learning Competences of Nursing students. This was a descriptive analyzed study, data were collected from April 3 to 14, 2017, study subjects were 208 nursing students in nursing colleges located C and I city, using self-reported questionnaires. The results of the study showed the mean scores of Helicopter parenting and critical thinking were $73.94{\pm}13.88$, $113.30{\pm}12.33$. And learning motivation, cognition, behavior were consisted in learning competence, learning cognition (F=3.62, p=.014) and learning behavior (F=4.07, p=.008) were statistically significant differences in school score. Helicopter parenting and critical thinking were a significant negative relationship (r=-.14, p=.043), according to critical thinking of learning cognition (r=.43, p<.001) and behavior (r=.42, p<.001) were a significant positive relationship. Based on the results, they were taken a baseline data for developing a Parent-Parenting Education Program to enhance on critical thinking and learning competence of nursing students and a Clinical Nursing Empowerment Program.
Objective : The objective of this study was to evaluatelong-term radiologic prognosis and characteristics of in-stent stenosis (ISS) after stent assisted coiling (SAC) for cerebral aneurysm and analyze its risk factors. Methods : Radiological records of 362 cases of SAC during 10 years were retrospectively reviewed. Patients were included in this study if they had follow-up angiogram using catheter selected angiography at least twice. All subjected were followed up from 12 months to over 30 months. Of 120 patients, 123 aneurysms were enrolled. Patient data including age, sex, aneurysm size, neck size, procedural complication, kinds of stent, ISS associated symptom, ruptured state, location of ISS, degree of ISS, radiologic prognosis of ISS, follow-up period of time, and medical comorbidities such as hypertension, diabetes mellitus (DM), dyslipidemia, and smoking were collected.Statistical comparisons of group clinical characteristics were conducted for the total population. Results : Among 123 casesof aneurysm, 22 cases (17.9%) of ISS were revealed on follow-up angiography. Multiple stenting was performed in three cases and intra-procedural rupture occurred in two cases. Most cases were asymptomatic and symptomatic stenosis was identified in only one case. Sixteen cases were ruptured aneurysm. Mild stenosis was observed in 11 cases. Moderate stenosis was found in eight cases and severe stenosis was identified in three cases. Mean timing of identification of ISS was 8.90 months. The most common type was proximal type. Most cases were improved or not changed on follow-up angiography. Only one case was aggravated from mild stenosis to occlusion of parent artery. Mean follow-up period was 44.3 months. We compared risk factors and characteristic between ISS group and non-ISS group using univariate analysis. Multiple stenting was performed for three cases (13.6%) of the ISS group and four cases (4.0%) of the non-ISS group, showing no statistical difference between the two groups (p=0.108). Additionally, the proportion of patients who had more than two risk factors among four medical risk factors (hypertension, DM, dyslipidemia, and smoking) was higher in the ISS group than that in the non-ISS group, the difference between the two was not statistically significant either (31.8% vs. 12.9%, p=0.05). Conclusion : Clinical course and long-term prognosis of ISS might be benign. Most cases of ISS could be improved or not aggravated. Control of medical co-morbidity might be important. To the best of our knowledge, our study had more cases with longer follow-up period of time than other reports.
Journal of the Korea Academia-Industrial cooperation Society
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v.20
no.1
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pp.259-267
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2019
The purpose of this study is to examine the inner side of those who raise the children, that is, the mothers, through the satisfaction with the parental roles and the child-rearing stress of them, with a view to emphasize the importance of an alternative for the mothers to cope with child-rearing stress and enhancing their satisfaction level with their role as a parent as well as the importance of social support in order to enhance the quality of rearing. For this purpose, the researcher conducted a survey with 312 mothers with preschool children of age 4 to 7 in Seoul and Gyeonggi Area from October 1, 2018 to October 15, 2018. To analyze the data, the researcher conducted frequency analysis, exploratory factor analysis, confirmatory factor analysis, confidence analysis, correlation analysis, structural equation model, and Sobet-test, using SPSS 22.0 and AMOS 22.0. The results of the analyses showed that social support and satisfaction with the parental role were significantly influenced by child-rearing stress, while social support influenced the satisfaction with the parental role significantly. Also, it turned out to have a mediating effect in the relationship between the mother's child-rearing stress and the satisfaction with the parental role. Based on these findings, the study highlights that the social network that is connected with the mothers may function to reduce the negative influence of child-rearing stress on the satisfaction with the parental role when the social network channels appropriate support, tangible assistance, appealing of difficulties, and encouragement.
The purpose of this study is to analyze the characteristics and stigma of children and adolescents, and the message types for them in NPO's fundraising advertisement videos in Korea. The subjects are 152 children and adolescents in 147 videos from seven major non-profit organizations. The analysis tools were constructed based on prior studies, and it was carried out after preliminary study. The results are as follows. First, the characteristics of children and adolescents indicated that the ratio of children and infants, main-role, poverty situation, one-parent family, and ward-role appeared the most. Second, 'general' was the highest in the stigma format, and 'hazard' and 'mark' was high in the challenge format. Third, the stigma message was the highest in message type. Finally, as of 2014, the ratio of stigma message and value-exclusion message increased, and the ratio of challenge message and mixed message decreased. The research suggests that a paradigm shift in the format of NPO's typical fundraising advertisement videos is necessary, as the videos may not protect the human rights of children and adolescents and may have negative effects such as stigma.
Journal of the Korea Academia-Industrial cooperation Society
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v.20
no.4
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pp.189-196
/
2019
The purpose of this study was to analyze the perception of child abuse by parents and early childhood teachers. For this purpose, a questionnaire survey was conducted with parents and early childhood teachers belonging to kindergarten in G district. The frequency, percentage, mean and standard deviation were calculated using the SPSS 21.0 program, and the t-test and the ${\chi}^2$ test were used to obtain data on child abuse(physical, language, emotion and thinking, neglect, preventive measure, improvement plan). The results of this study are as follows: First, the difference of perception about child abuse was higher in early childhood teachers than parents. Recognition of subordinate content is neglected in both parents and early childhood teachers, body, emotion and thinking, and language. Second, parent awareness of child abuse prevention education and participatory seminar education was more perceived than subcontent of preventive measures. The awareness and satisfaction of child abuse preventive education, the awareness of the legal role and function of the child protection agency, the mandatory reporting of child abuse, and the perception of the obligor were more perceived by early childhood teachers. Third, the subcontracting of the child abuse improvement measures, the provision of child abuse preventive education and provision of data, the actual situation of child abuse through mass media and promotion of measures, expansion of child abuse prevention education using mass media, Teachers were more aware of education. These results are expected to provide basic data on understanding and recognition of the prevention of child abuse by parents and early childhood teachers.
Although not many older Korean parents who take care of children with intellectual disabilities have been reported depression due to care-giving related stresses, little is known about how they could be protected from this. This study examines whether satisfaction with family relationships moderate the relationships between time demands, caregiving stress, and depression of older parents who take care of children with intellectual disabilities. The analyses were conducted based on data from the Korean National Survey on Individuals with Developmental Disabilities and their Families of 2011, and only a total of 276 parents, aged over 60 were examined. Multiple regression analysis shows that older parents with higher level of satisfaction with family relationships were less likely to be influenced by time demands of care-giving. This indicates that satisfaction with family relationships could buffer the relationships between time demands of care-giving, and depression. This finding suggests that satisfaction with family relationships is a protective factor, buffering the negative effects of time demands of care-giving and depression. This supports 'socio-emotional selectivity theory' which family relationships are important to older people. Therefore, it is highly recommended to develop practical intervention that can improve the level of satisfaction of family relationship of the older parents, and to make policy and institutional supplementation.
This study aims to examine how children's life satisfaction is interrelated with parents' life satisfaction longitudinally. Autoregressive cross-lagged model was employed to analyze the interrelationship between children's and parents' life satisfaction. This study used 6 waves (Elementary school 4th year to Middle school 3rd year) from the Korean Children and Youth Panel Study (KCYPS). The sample size was 1,963 families with child and parent. Key findings are as follows: First, children's and parents' life satisfaction, respectively, was found to influence each other during the study period. Second, parents' life satisfaction were found to have positive and significant cross-lagged impacts on children's life satisfaction. Third, similarly, children's life satisfaction was positively related to parents' life satisfaction longitudinally. The findings suggest that children's and parents' life satisfaction are interrelated in an autoregressive cross-lagged model. This study concludes with implications for enhancing children's and parents' life satisfaction.
Journal of the Korea Academia-Industrial cooperation Society
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v.20
no.9
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pp.362-371
/
2019
The purpose of this study is to recognize kindergarten teachers' experiences of child violence for identifying the site's needs for the implementation of preventive education in early childhood and to provide basic data on child violence. To this end, nine teachers were interviewed in-depth. From the study results, first, child violence-related child behavior, which usually occurs in kindergartens, includes physical assault, aggression, verbal violence, threats and threats, and bullying. Second, teachers usually use direct intervention laws in cases of child violence, but were finding it difficult to intervene properly with many tasks or high teacher-to-child ratios. Teachers recognized the need for lower teacher-to-child ratios and placement of each class burden. Third, teachers were aware of the need for prevention education for child violence targeting infants, and instigated the following appropriate interactions immediately after problem behavior occurred: large group activities, specific multimedia education data and parent education. Fourth, teachers are concerned about the possibility of problem behavior being learned and imitated through education in the implementation of prevention education for children's violence. Therefore, this study proposed the need to develop various teaching methods that could be applied to infant education sites, focusing on the types of child violence-related problem behaviors that occur in kindergartens.
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