• Parasites, Hosts and Diseases
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• 제26권4호
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• pp.275-284
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• 1988

1988년 2월 및 5월에 전라남도 여천군 남면 소재 연도 주민 1,011명 (남자 507명, 여자 504명)에 대하여 formalin-ether 침전법으로 장내 기생충 감염상환을 조사하고, 장내 윤충란 양성자 의 경우 감염강도를 E.P.G.로 측정한 바 다음과 같은 결과를 얻었다. 주민 1,011명중 398명 (39.4%)이 장내 기생충 양성자이었고 윤충 감염이 372명 (36.8%), 원충 감 염이 56명(5.5%)이었다. 검출된 기생충은 모두 10종으로서 편충란 양성자가 278명(27.5%)으로 가 장 많았고 그 다음이 회충 176명(17.4U, 불수정란 양성자 31명 포함), Taenia sp. 59명(5.8%)이었으며, 대장아메바 3.3%, 람블편모충 1.5%, 왜소아메바 0.8%, 왜소조충 0.4%이었고, 구충, 동 양모양선충 및 이질아메바가 각각 0.2%의 양성률을 보였다. 성별 감염률은 여자가 44.0%로 전 연령군에서 남자 34.7%보다 높았다. 연령별로는 40대가 46.0%로 가장 높았고 10세 미만이 33.1%로 가장 낮았다. 회충란 양성자중 149명의 E.P.G.는 평균 1,876(범위 200∼17,800), 편충 E.P.G. 는 검사자 242명에서 평균 327(범위 200∼1,600)이었다. 장내 기생 윤충의 양성자 572명중 단일 감염은 236명 (63.4%), 2종 감염은 129명 (34.7%), 3종 감염은 7명 (1.9%)이었고, 원충시스트 양성자 56명 중 단일 감염은 53명 (94.6%), 2종 감염은 3명 (5.4%)이었다. 이상의 결과로 보아 낙도 주민의 장내 기생충 감염률은 높음을 알 수 있으며 이의 퇴치를 위하여 생환 주변 환경의 개선, 집단 치료, 보건 교육 등 적극적인 관리가 시급히 수행되어야 할 것으로 생각된다.

• 한국수산과학회지
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• 제13권4호
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• pp.173-178
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• 1980

1980년 1월부터 동반 10월까지 군산 근교 미면 내초도에서 채집한 백합Meretrix lusoria, 바지락 Tapes japonica, 맞조개 Seten strictus을 조사하여 흡충류의 유충인 Bacciger harengulae의 cercaria의 높은 기생율을 보았다. 이 cercaria는 S자형으로 활발히 운동한다. 꼬리에는 양쪽에 27쌍의 강모(seta)을 가지며, 1개의 강모(setae)는 6개의 가락(조)으로 되어 있다. 체내에는 구흡반, 맹장, 흡복반과 2개의 정소가 뚜렷히 보이고, 큰 V자형의 관상 배설낭내에는 작고 큰 과립이 충만되고, 염세포식은 2[(3+3)+(3+3)]=24이다. 현재까지 보고된 유사한 ceraria를 비교하니 Cercaria pectinata와 같았다. 제2중간숙주를 거쳐 결숙주에 성충을 얻었으므로, Bacciger harengulae의 cercaria라 동정하였다. 내초도에서 채집한 패류중 맛조개, 바지락, 백합의 순으로 이 기생법의 기생률이 높았으나, 이들 조개류는 모두 2-3년생인큰 것이 많이 기생되어 있었다.

• 한국어병학회지
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• 제30권2호
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• pp.97-105
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• 2017

Parasitic leeches could directly (through causing poor growth, anemia and wound in the fish) and indirectly (by predisposition of the fish to secondary bacterial and fungal infections) affects their hosts. In the present study, fishes that were attacked by leeches in natural and experimental environment were studied. Pathologic samples were obtained from damages at the site of leech bite, as well as kidney and liver of the fish. Histopathological examination revealed numerous lesions at the site of leech bite including tissue demolition, detachment at the site of leech bite in the epidermis of epithelial tissue in the skin, destructed nucleus in epithelial cells of the skin plus necrosis in the damaged skin and weak inflammatory penetration to acute necrotic damages along with piercing dermis layer. Pathologic lesions in the kidney included some changes such as proliferation by increasing glomerular cells and membrane cells in capillary vein of the kidney, blood cell necrosis in kidney with infiltration of white blood cells mainly mononuclear and less polymorphonuclear which are the symptoms of anemia due to blood feeding and sucking by leeches. There was also a chronic kidney infection probably originated from another part of body such as skin. Moreover, leeches caused hemorrhagic anemia due to blood consumption of the hosts, which led to observation of immature red blood cells. Also results showed that diseases induced by leeched in fish could be acute or chronic, which depends on size of fish, species of leech and severity of infection.

• Parasites, Hosts and Diseases
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• 제50권4호
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• pp.295-299
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• 2012

The present study was performed to determine the infection status of anisakid larvae in marine fish collected from 3 sea areas of the Republic of Korea. Total 86 marine fish (8 species) collected from the East Sea (Goseong-gun, Gangwon-do), 171 fish (10 species) from the South Sea (Sacheon-si, Gyeongsangnam-do), and 92 fish (7 species) from the Yellow Sea (Incheon Metropolitan City) were examined by both naked eyes and artificial digestion method. Among the total of 349 fish examined, 213 (61.0%) were infected with 8 species of anisakid larvae, i.e., Anisakis simplex, 6 types of Contracaecum spp., and Raphidascaris sp., and the mean larval density was 13.8 per infected fish. Anisakid larvae were detected in 45 fish (52.3%) from the East Sea, 131 fish (76.6%) from the South Sea, and 37 fish (40.2%) from the Yellow Sea. The average numbers of larvae detected were 4.0, 16.6, and 15.9, respectively. Anisakis simplex larvae were detected in 149 fish (42.7%), and the mean larval density was 9.0 per infected fish. They were found in 26 fish (30.2%) collected from the East Sea, 96 fish (56.1%) from the South Sea, and 27 fish (29.3%) from the Yellow Sea. The average numbers of larvae detected were 2.9, 10.3, and 10.5, respectively. Conclusively, the present study suggests that the infection rate and density of anisakid larvae are more or less higher in the fish from the South Sea than those from the East Sea or the Yellow Sea.

• 한국동물위생학회지
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• 제35권2호
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• pp.111-117
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• 2012

This study investigated the occurrence of honeybee diseases in Incheon area, at the point of great widespread of sacbrood disease in the country. Sixteen resident beekeeping apiaries; 3 native honeybee and 13 European honeybee apiaries were selected for this research. Over 20 adult bees were evenly collected from the most colonies of each apiary three times (March, June, November) within a year. In this work, 13 honeybee diseases including 7 viral diseases, 2 bacterial diseases, 2 fungal diseases, and 2 parasitic diseases were detected by preliminary inspections and PCR. As a result, viral infections were confirmed at 34 among 48 apiaries (70.8%) over the entire examination period. Parasitic diseases showed the highest detection rate of 45.8%, which are detected in 44 among 96 cases. In the seasonal prevalence, 30 cases (15.6%) of 7 pathogens were detected from 14 apiaries in March, 50 cases (24.0%) of 9 pathogens and 56 cases (26.9%) of 9 pathogens were detected from all apiaries in June and November, respectively. Nosema was shown to be the most prevalent pathogen from March to November, followed by sacbrood virus (SBV) and stonebrood. The spread of SBV infection in Incheon would be under-estimated by the increasing of detection rate over the time. Especially, Chinese sacbrood virus was detected from 4 European honybee apiaries, but clinical symptoms were not found. No chalkbrood, acute bee paralysis virus, and chronic bee paralysis virus were detected in this study. The effective therapy and preventive measures should be prepared for beekeeping industry.

• Parasites, Hosts and Diseases
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• 제49권3호
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• pp.207-212
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• 2011

Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T. gondii antigens (SAG1, SAG2, GRA3, and GRA6) were produced and tested for the development of rapid diagnostic test (RDT). The proteins were expressed in Escherichia coli, affinity-purified, and applied onto the nitrocellulose membrane of the test strip. The recombinant SAG1 (rSAG1) showed the strongest antigenic activity and highest specificity among them. We also performed clinical evaluation of the rSAG1-loaded RDT in 182 cat sera (55 household and 127 stray cats). The kit showed 0.88 of kappa value comparing with a commercialized ELISA kit, which indicated a significant correlation between rSAG1-loaded RDT and the ELISA kit. The overall sensitivity and specificity of the RDT were 100% (23/23) and 99.4% (158/159), respectively. The rSAG1-loaded RDT is rapid, easy to use, and highly accurate. Thus, it would be a suitable diagnostic tool for rapid detection of antibodies in T. gondii-infected cats under field conditions.

• Parasites, Hosts and Diseases
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• 제57권6호
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• pp.587-593
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• 2019

Excavation (2008-2014) carried out under the Uffizi Gallery (Florence, Italy) led to the discovery of 75 individuals, mostly buried in multiple graves. Based on Roman minted coins, the graves were preliminarily dated between the second half of the 4th and the beginning of the 5th centuries CE. Taphonomy showed that this was an emergency burial site associated with a catastrophic event, possibly an epidemic of unknown etiology with high mortality rates. In this perspective, paleoparasitological investigations were performed on 18 individuals exhumed from 9 multiple graves to assess the burden of gastrointestinal parasitism. Five out of eighteen individuals (27.7%) tested positive for ascarid-type remains; these are considered as "decorticated" Ascaris eggs, which have lost their outer mammillated coat. Roundworms (genus Ascaris) commonly infest human populations under dire sanitary conditions. Archaeological and historical evidence indicates that Florentia suffered a period of economic crisis between the end of 4th and the beginning of the 5th centuries CE, and that the aqueduct was severely damaged at the beginning of the 4th century CE, possibly during the siege of the Goths (406 CE). It is more than plausible that the epidemic, possibly coupled with the disruption of the aqueduct, deeply affected the living conditions of these individuals. A 27.7% frequency suggests that ascariasis was widespread in this population. This investigation exemplifies how paleoparasitological information can be retrieved from the analysis of sediments sampled in cemeteries, thus allowing a better assessment of the varying frequency of parasitic infections among ancient populations.

• 한국어병학회지
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• 제37권1호
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• pp.97-110
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• 2024

The Serranidae is high-quality fish species with good meat quality and is traded at high price, and is attracting attention in South Korea as a cultured species that creates high added value. However, the high-density fish farming for mass production increases the risk of mass mortality due to infectious diseases, leading to enormous economic losses. Therefore, in order to safely prevent and protect farmed fish from serious infectious diseases, it is necessary to conduct disease monitoring on a regular basis. In this study, Hyporthodus septemfasciatus, Epinephelus moara, and the hybrid longtooth grouper (E. moara ♀×E. lanceolatus ♂) were collected once a month from fish farm of Garorim and Aquabiotech Co., Ltd for a total of six months, from July to December 2023. We investigated infections of five species of bacterial diseases, including Flavobacterium columnare, six species of viral diseases, including LCDV (lymphocystis disease virus), and parasitic pathogens in grouper farms. As the result, Vibrio vulnificus and V. harveyi were detected in H. septemfasciatus in August, in the case of viral diseases, NNV was detected in H. septemfasciatus from July to August using RT-PCR or PCR. Finally, In the case of parasitic diseases, Tricodina sp. was detected in E. moara and the hybrid longtooth grouper from August to December.

• 한국해양학회지:바다
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• 제7권3호
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• pp.181-194
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• 2002

기생은 공생하는 두 생물사이에 한쪽(기생생물)은 이득을 얻는 반면에 다른 한쪽(숙주)은 해를 입는 관계를 말한다. 이러한 기생관계가 생물학적으로 적조를 제어하기 위한 하나의 방법으로서 응용될 수 있으며, 특히 적조생물을 숙주로 하는 기생성 와편모류인 Amoebophrya spp.는 그 잠재력이 큰 것으로 오랫동안 주목받아 왔다. Amoebophrya spp.는 유독성 및 유해성 적조를 일으키는 종을 포함하여 24속 40여종 이상의 와편모류들을 감염시킬 수 있는 것으로 알려져 있다 기생성 와편모류 Amoebophrya는 포자(dinospore)의 분산 및 감염 단계,감염 후 숙주 세포내에서 성장단계 (trophont), 그리고 숙주 세포 밖의 생식단계(vermiform) 등의 간단한 생활사를 가지고 있다. 최근의 연구결과에 의하면, 분산 및 감염을 담당하는 dinospore는 각 숙주-기생생물 관계에 따라 감염능력, 생존능력 및 감염 성공률 등 서로 다른 생물학적 차별성을 보인다. 각 숙주기생생물 관계 기원 dinospores의 서로 다른 생물학적 특성, 교차 감염실험 및 분자생물학적 연구결과 등으로 보아 Amoebophrya ceratil가 숙주 특이성이 없는 단일종이라는 이전의 주장보다는 아마도 숙주 특이성을 갖고 있는 여러 분류군으로 구성된 종 복합체 (species complex)라는 가설이 타당하다. 숙주 와편모류들은 기생성 와편모류 Amoebophrya에 의해 일단 감염되고 나면 더 이상 성장하지 못하고 생식능력을 잃고 모두 사망하게 되는데, 이 감염 과정동안 해당 플랑크톤 생태계에 대한 영향은 다양하게 나타난다. 독립영양성 와편모류들이 감염될 경우 그들의 광합성 능력이 크게 영향을 받으며, 감염위치(핵 또는 세포질)에 따라서 영향받는 속도가 다르다. 이 기생성 와편모류에 의한 감염은 광주성 및 유영속도 등에 영향을 미쳐서 숙주 와편모류의 일주 수직이동 양상과 같은 동태에도 커다란 영향을 끼칠 수 있다. 기생성 와편모류의 감염으로 인한 숙주의 사망은 자연 사망할 때보다 시간적으로 더 빨리 숙주로부터 다량의 유기물이 방출되게 할 수 있을 뿐만 아니라, 기생생물에 의한 감염은 감염기간 동안에도 숙주로부터 상당한 양의 용존 유기물이 인위적으로 방출되게 할 가능성이 있다. 기생생물에 감염시에는 상대적으로 크기가 큰 숙주 와편모류의 생체량이 사망과 동시에 크기가 작은 수많은 포자들로 생물학적 전환이 일어나고, 이어서 이들 포자들은 섬모류 등의 포식자들에게 먹이로서 이용i소화될 수 있으므로, 숙주 와편모류가 지녔던 물질과 에너지가 미소생물고리내에서 보다 오랜기간 동안 머무를 수 있는 가능성이 더욱 커질 것으로 생각된다 다양한 숙주-기생생물 관계에 대하여 다양한 물리, 화학적 환경요인과 여타의 생물학적 요인들이 어떠한 영향을 미치는지에 대한 보다 심도있는 연구는 플랑크톤 생태학과 적조역학에서 기생성 와편모류의 역할을 보다 잘 이해하도록 하는데 기여할 것으로 기대된다.

• Parasites, Hosts and Diseases
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• 제48권1호
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• pp.75-78
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• 2010

The present study investigated characteristics of 24 parasite infection cases detected during colonoscopy in a regional hospital from January 2001 to December 2008. Sixteen patients were confirmed with Trichuris trichiura infection, 6 patients were with Ascaris lumbricoides infection, 1 patient with Enterobius vermicularis infection, and 1 patient with Anisakis infection. Among them, 7 patients (43.8%) were asymptomatic. Colonoscopy findings were normal in 18 patients (75.0%). Among the patients with T. trichiura infection, colonoscopy showed several erosions in 2 patients (8.3%) and non-specific inflammation of the affected segment of the colon in 3 patients (12.5%). In 1 patient with anisakiasis, colonoscopy revealed a markedly swollen colonic wall. Stool examinations were performed before treatment in 7 patients (29.2%) and were all negative for parasite eggs or worms. These results suggest that colonoscopy is a useful diagnostic approach for parasitic infections even for asymptomatic patients and for patients with negative stool examinations.