• Parasites, Hosts and Diseases
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• 제41권2호
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• pp.89-96
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• 2003

Among the panel of monoclonal antibodies (mAb) against Toxoplasma gondii, mAb of Tg621 (Tg621) clone blotted 38 kDa protein which localized in the cytoplasm of tachyzoites by immunofluorescence microscopy The protein was not released into the parasitophorous vacuole during or after invasion. The cDNA fragment encoding the protein was obtained by screening a T. gondii cDNA expression library with Tg621. The full length cDNA sequence was completed with 5’-RACE as 1,592 bp, which contained open reading frame of 942 bp. The deduced amino acid sequence of Tg621 consisted of a polypeptide of 313 amino acids, with significant homology to ribosomal P proteins (RPP) of other organisms especially high to those of apicomplexan species. The expressed and purified TgRPP was assayed in western blot with the sera of toxoplasmosis patients and normal sera, which resulted in the 74.0% of positive reactions in toxoplasmosis patients whereas 8.3% in normal group. Therefore, the antibody formation against TgRPP in toxoplasmosis patients was regarded as specific for T. gondii infection and suggested a potential autoantibody.

• 한국수산과학회지
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• 제13권4호
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• pp.173-178
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• 1980

1980년 1월부터 동반 10월까지 군산 근교 미면 내초도에서 채집한 백합Meretrix lusoria, 바지락 Tapes japonica, 맞조개 Seten strictus을 조사하여 흡충류의 유충인 Bacciger harengulae의 cercaria의 높은 기생율을 보았다. 이 cercaria는 S자형으로 활발히 운동한다. 꼬리에는 양쪽에 27쌍의 강모(seta)을 가지며, 1개의 강모(setae)는 6개의 가락(조)으로 되어 있다. 체내에는 구흡반, 맹장, 흡복반과 2개의 정소가 뚜렷히 보이고, 큰 V자형의 관상 배설낭내에는 작고 큰 과립이 충만되고, 염세포식은 2[(3+3)+(3+3)]=24이다. 현재까지 보고된 유사한 ceraria를 비교하니 Cercaria pectinata와 같았다. 제2중간숙주를 거쳐 결숙주에 성충을 얻었으므로, Bacciger harengulae의 cercaria라 동정하였다. 내초도에서 채집한 패류중 맛조개, 바지락, 백합의 순으로 이 기생법의 기생률이 높았으나, 이들 조개류는 모두 2-3년생인큰 것이 많이 기생되어 있었다.

• Parasites, Hosts and Diseases
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• 제51권2호
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• pp.207-212
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• 2013

Chronic diarrhea with a 35 kg weight loss (75 kg to 40 kg) occurred during 2 years in an alcoholic patient was diagnosed with Isospora belli infection in the Republic of Korea. The patient, a 70-year old Korean male, had been a heavy drinker for more than 30 years. He was admitted to the Seoul National University Hospital because of long-standing diarrhea and severe weight loss. He had an increased white blood cell (WBC) count with high peripheral blood eosinophilia (36.8-39.9%) and lowered protein and albumin levels but without any evidence of immunosuppression. A parasitic infection was suspected and fecal examination was repeated 3 times with negative results. Peroral endoscopy with mural biopsy was performed in the upper jejunum. The biopsy specimens revealed villous atrophy with loss of villi together with various life cycle stages of I. belli, including trophozoites, schizonts, merozoites, macrogamonts, and microgamonts. The patient was treated successfully with oral doses of trimethoprim 160-320 mg and sulfamethoxazole 800-1,600 mg daily for 4 weeks. A follow-up evaluation at 2.5 years later revealed marked improvement of body weight (68 kg), increased protein and albumin levels, and normal WBC count with low eosinophils (3.1%). This is the first clinical case of isoporiasis with demonstration of various parasitic stages in the Republic of Korea.

• Journal of Preventive Medicine and Public Health
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• 제10권1호
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• pp.150-154
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• 1977

In the diagnosis of parasitic helminthic diseases, the value of examining and identifying the parasitic eggs and/or adult parasites from patient's urine or stool are well appreciated. However, these methods have a limited value in the diagnosis of tissue or intracellular parasitism, and we have to rely on supplementary methods such as immune-serological test. The author tested the value of gel precipitation reactions as a diagnostic method of clonorchiasis by observing the appearance of bands in rats experimentally infected with Clonorchis sinensis, And the therapeutic effect of CIBA 35'058-Ba was evaluated by this serological method. The antigen was prepared from the adult worms infected in rabbits by Tsuji method. Rats infected with 40 metacercariae each were bled on 7,14,21,26,28,39,42(43),49(53) days after infection to observe the appearance of precipitin bands by both Oucterlony method and immunoelectrophoresis. Fifteen rats were separately infected and treated with CIBA 35'058-Ba in dose of 15mg/kg of body weight. The following results were obtained: 1. It was observed that there exist individual variations in the appearance of the first precipitin band with the range of 2-4 weeks after infection. 2. The number of precipitin bands was increased until 6-7 weeks after infection. In all cases, 3 precipitin bands were appeared by Oucterlony method and 6-7 bands were appeared by immunoelectrophoresis after 6-7 weeks of infecion. 3. It was hardly possible to notice any change in the number of bands after the administration of CIBA 35'058-Ba. This result suggested that the drug has no effect on clonorchiasis which was confirmed by the autopsy of the experimental rats.

• Parasites, Hosts and Diseases
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• 제56권6호
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• pp.633-637
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• 2018

Present study was performed to survey on infection status of Isthmiophora hortensis (formerly Echinostoma hortense) metacercariae (IhMc) in dark sleepers, Odontobutis spp., from some water systems of the Republic of Korea. A total of 237 Odontobutis spp. was collected in the water systems of 5 rivers, i.e., Mangyeong-gang (gang means river), Geum-gang, Tamjin-gang, Seomjin-gang, and Nakdong-gang. They were all examined with artificial digestion method for 5 years (2013-2017). A total of 137 (57.8%) Odontobutis spp. were infected with 14.8 IhMc in average. The prevalence was the highest in Nakdong-gang areas (62.9%) and followed by in Mangyeong-gang (57.1%), Geum-gang (56.3%), Tamjin-gang (54.8%), and Seomjin-gang (53.9%) areas. Metacercarial densities were 28.1 (Geum-gang), 13.9 (Mangyeong-gang), 13.3 (Nakdong-gang), 13.1 (Tamjin-gang), and 2.3 (Seomjin-gang) per infected fish. Especially, in case of Yugucheon (cheon means stream), a branch of Geum-gang, IhMc were detected in all fish (100%) examined and their density was about 48 per fish. By the present study, it was confirmed that the infection status of IhMc is more or less different by the surveyed areas and the dark sleepers, Odontobutis spp., are suitable fish hosts of I. hortensis.

• Parasites, Hosts and Diseases
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• 제59권3호
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• pp.265-271
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• 2021

We surveyed on the infection status of zoonotic trematode metacercariae (ZTM) in freshwater fishes from Soyang-cheon (a branch stream of Mangyeong-gang) in Wanju-gun, Jeollabuk-do, the Republic of Korea. A total of 927 fishes were individually examined with the artificial digestion method during 2013-2015 (462 fish in 15 spp.) and 2018-2019 (465 fish in 25 spp.). Clonorchis sinensis metacercariae were detected in 207 (31.4%) out of 659 fishes in 14 positive fish species (PFS), and their mean intensity was 114 per fish infected (PFI). Metagonimus spp. metacercariae were found in 302 (37.4%) out of 808 fishes in 21 PFS, and their mean intensity was 12 PFI. Centrocestus armatus metacercariae were detected in 222 (59.0%) out of 376 fishes in 12 PFS, and their mean intensity was 383 PFI. Echinostoma spp. metacercariae were found in 139 (22.1%) out of 628 fishes in 10 PFS, and their mean intensity was 7 PFI. Clinostomum complanatum metacercariae were detected in 14 (6.5%) out of 214 fishes in 4 PFS, and their mean intensity was 2.4 PFI. Metorchis orientalis metacercariae were detected in 36 (13.5%) out of 267 fishes in 5 PFS, and their mean intensity was 4.3 PFI. Conclusively, the prevalence and infection intensity of ZTM is generally not so high in fishes from Soyang-cheon. However, those of C. sinensis metacercariae are more or less higher in 2 fish species, Pungtungia herzi and Sarcocheilichthys variegatus wakiyae.

• 한국동물위생학회지
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• 제21권4호
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• pp.431-437
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• 1998

Argulus spp, a small group of parasitic curstaceans called Branchiura, is particularly important in freshwater acquaculture. We investigated the infection of Argulus to several different species of fishes and therapy effect of masoten. Argulus japonicus was collected from Okjeong lake, Chonbuk province and experlmen-tally infected to freshwater fishes from July to August 1998. The results were as follows ; 1. The shape of a Agulus is an oval and dorsoventrally flattened, with first maxillae and second maxillae, and four pairs of legs. 2. The size of the adult stage of A japonicus was 3.2-5.0$\times$2.3-2.9mm in female and 1.5-3.1$\times$1.6-2.9mm in male. 3. In the trial of experimental infection with A japonicus to non-scaled fishes, Parasilurus asotus and Ictalurus punctalus, were infected and some of them died after severe infection. However, A japonica was not infected at all. 4. Masoten treatment for A japonicus was effective at 0.2 ppm once a day for 3 days and parasites on the surface of fishes disappeared after five days.

• 한국임상수의학회지
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• 제20권3호
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• pp.413-418
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• 2003

This survey of endoparasite infections among stray cats in Korea has been carried out during November 1995 to October 1997. A total of 215 stray cats were collected from SeoulㆍGyeonggi (54), Gangwon (38), Chungchong (34), Jeonla (38), and Gyeoungsang (51) areas. The positive rate of endoparasites was 184 (85.6%) cats among 215. Of isolated parasites, nematodes were the most often detected parasites especially Toxocara cati (102 cats), Ancylostoma tubaeformae (9 cats). The next most often detected parasites were cestodes (60/215; 27.9%) sepecially Spirometra erinaecei (34 cats), Taenia taeniaformis (26 cats). Opisthorchis tenuicollis was found in bile duct (9 cats) and Isospora spp. were identified in 41 cats (19.1 %). In mixed parasite infection, the rate of infections of nematodes, trematodes and protozoas was 3.3%, nematodes and cestodes with 8.8%, nematodes and protozoa with 4.2%, cestodes and protozoa with 1.9%. In parasitic infection by a considering age category, age from 2 to 4 years had the highest infection (92.3%), 1-2 years were 87.6%, over 4 years was 80.0%. 79.6 percents of the stray cats in SeoulㆍKyounggi areas were ranked the 1st in parasite infection. The next was Kyoungsang 76.5%, Kangwon 65.8%, Cholla 63.2% and Chungchong 61.8% in order. Considering age categories from birth to greater than 4 years, parasite infections were most prevalent in cats 2 to 4 years old; other ages seemed to have no effect on prevalence of parasitism. Also sex seemed to have no effect on prevalence of parasitism. In endoparasite infection, nematode and cestode infections were most frequently found, trematode infections were low, coccidiosis was showed very often.

• 한국동물위생학회지
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• 제17권1호
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• pp.25-31
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• 1994

In order to investigate the internal parasitic infection, fecal samples were collected from weaning pig(n=123), porker(n=418) and sow(n=121) in 49 sawdust fermentative pigsty of Chonbuk district. The prevalence and identification of internal parasites were determined by the fecal examination using the floatation and /or sedimentation methods and microscopical examination, respectively. The results were obtained as follows ; 1. The detection rate of parasite - eggs from 662 fecal samples was 86.6%. 2. The infection rate of parasite-egg 96.4% in porker, 76.9% in sow, 62.6% in weaning pig, in order. 3. In the concern of mired infection such as single, double triple and quadraple, the rate was 42.3%, 28.7%, 12.2% and 3.3%, respectively. 4. Ten kinds of the detected eggs were isolated from 662 fecal samples. They were classified as Balantidium coli (63.6%), Trichuris suis(24.8%), isospora spp.(23.5%), Oesoohangostomum spp.(17.8%), Ascaris suum(11.8%), Hyostronylus rubiddus (2.8%), strongyloides spp. (1.7%), Gnathostoma spp. (1.5%), Stephanurus dentatus(1.3%) and Metastrongylus spp. (0.7%), in order.

• 한국임상수의학회지
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• 제37권4호
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• pp.223-226
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• 2020

Cytauxzoonosis is caused by Cytauxzoon felis (C. felis) in wild and domestic cats. However, cytauxzoonosis is uncommon in Asia. Additionally, clinical reports of C. felis infection along with associated complications are rare. A seven-year-old neutered male Maine Coon cat was presented with acute dyspnea and lethargy despite the absence of a history of overseas travel. Mild regenerative anemia and autoagglutination were detected in hematological investigations. The parasitic and viral PCR assays revealed infection with C. felis and feline immunodeficiency virus (FIV). Thoracic radiographs showed pleural effusion with secondary bacterial infection. Ultimately, a diagnosis of infection-induced secondary immune-mediated hemolytic anemia (IMHA) and pyothorax was established. The cat was treated with a combination of atovaquone, prednisolone, and cyclosporine over 6 months and the final treatment was completed 8 months after initiation of therapy. This is the first report of its kind demonstrating successful management of feline IMHA and fatal pyothorax induced by FIV and C. felis in South Korea.