• Proceedings of the Korean Society of Toxicology Conference
• /
• 2006.11a
• /
• pp.65-74
• /
• 2006

Modem drug discovery requires rapid pharmacokinetic evaluation of chemically diverse compounds for early candidate selection. This demands the development of analytical methods that offer high-throughput of samples. Naturally, liquid chromatography / tandem mass spectrometry (LC-MS/MS) is choice of the analytical method because of its superior sensitivity and selectivity. As a result of the short analysis time(typically 3-5min) by LC-MS/MS, sample preparation has become the rate- determining step in the whole analytical cycle. Consequently tremendous efforts are being made to speed up and automate this step. In a typical automated 96-well SPE(solid-phase extraction) procedure, plasma samples are transferred to the 96-well SPE plate, internal standard and aqueous buffer solutions are added and then vacuum is applied using the robotic liquid handling system. It takes only 20-90 min to process 96 samples by automated SPE and the analyst is physically occupied for only approximately 10 min. Recently, the ultra-high flow rate liquid chromatography (turbulent-flow chromatography)has sparked a huge interest for rapid and direct quantitation of drugs in plasma. There is no sample preparation except for sample aliquotting, internal standard addition and centrifugation. This type of analysis is achieved by using a small diameter column with a large particle size(30-5O ${\mu}$m) and a high flow rate, typically between 3-5 ml/min. Silica-based monolithic HPLC columns contain a novel chromatographic support in which the traditional particulate packing has been replaced with a single, continuous network (monolith) of pcrous silica. The main advantage of such a network is decreased backpressure due to macropores (2 ${\mu}$m) throughout the network. This allows high flow rates, and hence fast analyses that are unattainable with traditional particulate columns. The reduction of particle diameter in HPLC results in increased column efficiency. use of small particles (<2 urn), however, requires p.essu.es beyond the traditional 6,000 psi of conventional pumping devices. Instrumental development in recent years has resulted in pumping devices capable of handling the requirements of columns packed with small particles. The staggered parallel HPLC system consists of four fully independent binary HPLC pumps, a modified auto sampler, and a series of switching and selector valves all controlled by a single computer program. The system improves sample throughput without sacrificing chromatographic separation or data quality. Sample throughput can be increased nearly four-fold without requiring significant changes in current analytical procedures. The process of Bioanalytical Method Validation is required by the FDA to assess and verify the performance of a chronlatographic method prior to its application in sample analysis. The validation should address the selectivity, linearity, accuracy, precision and stability of the method. This presentation will provide all overview of the work required to accomplish a full validation and show how a chromatographic method is suitable for toxirokinetic sample analysis. A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method developed to quantitate drug levels in dog plasma will be used as an example of tile process.

• Proceedings of the Korean Society of Propulsion Engineers Conference
• /
• 2003.05a
• /
• pp.32-33
• /
• 2003

Mechanism of a periodic oscillation of shock-induced combustion over a two- dimensional wedges and axi-symmetric cones were investigated through a series of numerical simulations at off-attaching condition of oblique detonation waves(ODW). A same computational domain over 40 degree half-angle was considered for two-dimensional and axi-symmetric shock-induced combustion phenomena. For two-dimensional shock-induced combustion, a 2H2+02+17N2 mixture was considered at Mach number was 5.85with initial temperature 292 K and initial pressureof 12 KPa. The Rankine-Hugoniot relation has solution of attached waves at this condition. For axi-symmetric shock-induced combustion, a H2+2O2+2Ar mixture was considered at Mach number was 5.0 with initial temperature 288 K and initial pressure of 200 mmHg. The flow conditions were based on the conditions of similar experiments and numerical studies.[1, 3]Numerical simulation was carried out with a compressible fluid dynamics code with a detailed hydrogen-oxygen combustion mechanism.[4, 5] A series of calculations were carried out by changing the fluid dynamic time scale. The length wedge is varied as a simplest way of changing the fluid dynamic time scale. Result reveals that there is a chemical kinetic limit of the detached overdriven detonation wave, in addition to the theoretical limit predicted by Rankine-Hugoniot theory with equilibrium chemistry. At the off-attaching condition of ODW the shock and reaction waves still attach at a wedge as a periodically oscillating oblique shock-induced combustion, if the Rankine-Hugoniot limit of detachment isbut the chemical kinetic limit is not.Mechanism of the periodic oscillation is considered as interactions between shock and reaction waves coupled with chemical kinetic effects. There were various regimes of the periodicmotion depending on the fluid dynamic time scales. The difference between the two-dimensional and axi-symmetric simulations were distinct because the flow path is parallel and uniform behind the oblique shock waves, but is not behind the conical shock waves. The shock-induced combustion behind the conical shockwaves showed much more violent and irregular characteristics.From the investigation of characteristic chemical time, condition of the periodic instability is identified as follows; at the detaching condition of Rankine-Hugoniot theory, (1) flow residence time is smaller than the chemical characteristic time, behind the detached shock wave with heat addition, (2) flow residence time should be greater than the chemical characteristic time, behind an oblique shock wave without heat addition.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.26 no.2
• /
• pp.63-81
• /
• 2021

In this study, we investigated the tide-induced flow patterns near the ocean outfall of the Jeju and Bomok Wastewater Treatment Plants (WTP) in Jeju Island by using measurements of Acoustic Doppler Current Meter (ADCP) and a numerical experiment with inserting passive tracer into a regional ocean model. In late spring of 2018, the ADCP measurements showed that tidal currents dominate the flow patterns as compared to the non-tidal components in the outfall regions. According to harmonic analysis, the dominant type of tides is mixed of diurnal and semi-diurnal but predominantly semidiurnal, showing stronger oscillations in the Jeju WTP than those in the Bomok WTP. The tidal currents oscillate parallel to the isobath in both regions, but the rotating direction is different each other: an anti-clockwise direction in the Jeju WTP and a clockwise in the Bomok WTP. Of particular interest is the finding that the residual current mainly flows toward the coastline across the isobath, especially at the outfall of the Bomok WTP. Our model successfully captures the features of tidal currents observed near the outfall in both regions and indicates possibly high persistent pollutant accumulation along the coasts of Bomok.

• Proceedings of the KSME Conference
• /
• 2007.05b
• /
• pp.3418-3422
• /
• 2007

The neutron imaging technique was used to investigate the water discharge characteristics at PEMFC. Prior to investigation of water discharge characteristics, the linear attenuation coefficient for water at Neutron Radiography Facility (NRF) was calibrated. The feasibility test apparatus was consisted of pressurized air and water in order to simulate the actual operating PEMFC. The feasibility tests have been performed at 1-parallel serpentine type with 100 $cm^2$ active area and different air flow rate (1, 2, and 4 lpm). The total water volume variations at each condition were calculated from the neutron images. The water at channel is well discharged as soon as supplying the pressurized air into the PEMFC. However, because the water at MEA isn't removed the total water volume is constant after 150. Therefore more effective method is needed in order to discharge water at MEA, and the neutron imaging technique is helpful for it.

• Proceedings of the KSME Conference
• /
• 2007.05b
• /
• pp.1974-1979
• /
• 2007

In this study, Ice slurry generator heat transfer characteristics are experimentally investigated for the ice slurry generating system with scraper which is pneumatically operated. The ice slurry generator has the same shape as the vertical double tube type heat exchanger. Refrigerant is flowing in the outside tube and ethylene glycol solution in the inside tube. Refrigerant and solution water are parallel flow type which entering bottom of generator and leaving top of generator. The experimentations are conducted under the various test conditions such as compressor speed and cooling water temperature. For the above experimental conditions, heat transfer characteristics of the ice slurry generating system are evaluated in terms of the overall heat transfer coefficient and the heat transfer rate. And the experimental results show that the heat transfer rate of the system is increased as the compressor speed increases and the cooling water temperature decreases.

• Proceedings of the KSR Conference
• /
• 2011.05a
• /
• pp.7-13
• /
• 2011

Simulation study were performed for ventilation capability effect on the smoke spread in the deeply-underground subway station(DUSS). Singeumho station(The line # 5, Depth: 46m) was modeled and were analyzed for smoke-spread speed difference between the originally-designed-ventilation-capacity and the measured-ventilation-capacity. Field test data for actual fan in DUSS was applied as a boundary condition of a simulation. The whole station was covered in this analysis and total of 4 million grids were generated for this simulation. The fire-driven flow was analyzed case by case to compare the smoke-spread effects. In order to enhance the efficiency of calculation, parallel processing by MPI was employed and large eddy simulation method in FDS code was adopted.

• Proceedings of the KSR Conference
• /
• 2011.10a
• /
• pp.2885-2890
• /
• 2011

Simulation study were performed for fire location effect on the smoke spread in the deeply-underground subway station(DUSS). In this research, Shingumho station (The line # 5, Depth: 46m) has been selected as case-study for the analysis of smoke-spread effect with the different fire location. Field test data measured for actual fan in DUSS was applied as a condition of a simulation. The whole station was covered in this analysis and 4 million grids were generated for this simulation. The fire driven flow was analyzed case by case to compare the smoke-spread effect according to the fire location. In order to enhance the efficiency of calculation, parallel processing by MPI was employed and LES(large eddy simulation) method in FDS code was adopted.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.27 no.4
• /
• pp.195-200
• /
• 2015

The thermal performance in the channels with two-wall rectangular convergent/divergent cross-sectional areas along the axial distance was investigated experimentally. The ribbed rectangular convergent/divergent channels were manufactured with a fixed rib height (e) = 10 mm and the ratio of rib spacing (p) to height (e) = 10. Three different parallel angled ribs (a = $30^{\circ}$, $45^{\circ}$, and $60^{\circ}$) were each placed on the channel's one sided wall only. The convergent channel of $D_{ho}/D_{hi}=0.67$ and the divergent channel of $D_{ho}/D_{hi}=1.49$ were considered. The ribbed divergent channel produced better thermal performance than the ribbed convergent channel in three different restrictions; identical flow rate, identical pumping power, and identical pressure loss.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.28 no.6
• /
• pp.774-782
• /
• 2004

This article presents a numerical and experimental investigation for the single-phase forced laminar convective heat transfer through arrays of micro-channels in micro heat exchangers to be used for cooling power-intensive semiconductor packages, especially the stacked multi-chip modules. In the numerical analysis, a parametric study was carried out for the parameters affecting the efficiency of heat transfer in the flow of coolants through parallel rectangular micro-channels. In the experimental study, the cooling performance of the micro heat exchanger was tested on prototypes of stacked multi-chip modules with difference channel dimensions. The simulation results and the experiment data were acceptably accordant within a wide range of design variations, suggesting the numerical procedure as a useful method for designing the cooling mechanism in stacked multi-chip packages and similar electronic applications.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.30 no.2 s.245
• /
• pp.136-143
• /
• 2006

A particle image velocimetry (PlV) technique has been applied to measure the quantitative flow field characteristics behind a road vehicle with/without an air spoiler attached on its trunk and to estimate its effect on the wake. A vehicle model scaled in the ratio of 1/43 is set up in the mid-section of a closed-loop water tunnel. The Reynolds number based on the vehicle length is $10^5$. To investigate the three-dimensional structure of the recirculation zone and vortices, measurements are carried out on the planes both parallel and perpendicular to the free stream, respectively. The results show significant differences in the recirculation region and the vorticity distributions according to the existence of the air spoiler. The focus and the saddle point, appearing just behind the air spoiler, are disposed differently along the spanwise direction. Regarding the streamwise vortices, the air spoiler produces large wing tip vortices. They have opposite rotational directions to C-pillar vortices which are commonly observed in case that the air spoiler is absent. The wing tip vortices generate the down-force and as a result, they can make the vehicle more stable in driving.