• Mycobiology
• /
• v.37 no.4
• /
• pp.272-276
• /
• 2009

In this study, eight soil samples were collected from organic and conventional farms in a central area of South Korea. Spore communities of arbuscular mycorrhizal fungi (AMF) and glomalin, a glycoprotein produced by AMF, were analyzed. Spores of Glomus clarum, G. etunicatum, G. mosseae, G. sp., Acaulospora longula, A. spinosa, Gigaspora margarita, and Paraglomus occultum were identified at the study sites, based on morphological and molecular characteristics. While Acaulospora longula was the most dominant species in soils at organic farms, Paraglomus occultum was the most dominant species in soils at conventional farms. Species diversity and species number in AMF communities found in soils from organic farms were significantly higher than in soils from conventional farms. Glomalin was also extracted from soil samples collected at organic and conventional farms and was analyzed using both Bradford and enzyme-linked immunosorbent assays. The glomalin content in soils from organic farms was significantly higher than in soils from conventional farms. These results indicate that agricultural practices significantly affect AMF abundance and community structure.

• The Korean Journal of Mycology
• /
• v.39 no.1
• /
• pp.1-6
• /
• 2011

This study was conducted to reveal the diversity of arbuscular mycorrhizal fungi (AMF) in Mt. Munan, based on the morphological and molecular characters. Soil and root samples were collected from the rhizosphere of host plants including Lindera obtusiloba, Stephanandra incisa, Styrax obassis and Symplocos sawafutagi and AMF were trap-cultured with Sorghum bicolor as a host plant in a greenhouse. The spores were extracted from the cultured soils and five species were identified using morphological and molecular characteristics; Acaulospora longula, A. mellea, Ambispora leptoticha, Gigaspora margarita and Paraglomus occultum. The distribution of AMF showed different trends according to host plants. The dominant AMF species were A. longula in L. obtusiloba, A. leptoticha in S. incisa, S. obassis and S. sawafutagi.

• The Korean Journal of Mycology
• /
• v.40 no.1
• /
• pp.1-6
• /
• 2012

In this study, soil samples were collected from cultivated fields of 1-5 year old Korean ginseng in Geumsan, Korea. Spores of arbuscular mycorrhizal fungi were extracted from soils and identified using morphological characteristics and 18s rDNA sequences of the spores. Total 10 species of AMF were identified: Acaulospora longula, Archaeospora trappei, Glomus caledonium, Glomus etunicatum, Glomus intraradices, Glomus mosseae, Glomus sp., Paraglomus occultum, Paraglomus brasilianum, and Scutellospora heterogama. Relative abundance of spores of A. trappei were increased with increase of cultivation period of the ginseng. However, relative abundance of other species of AMF and Shannon diversity (H') of AMF were significantly decreased with the increase of cultivation periods of the ginseng.

• The Korean Journal of Mycology
• /
• v.45 no.4
• /
• pp.284-291
• /
• 2017

In this study, arbuscular mycorrhizal fungi (AMF) were isolated from rhizosphere soils of Dokdo Island. Based on their morphological characteristics and 18S rDNA sequence analysis, eight species belonging to seven genera were identified: Acaulospora longula, A. mellea, Claroideoglomus claroideum, Diversispora aurantia, Funneliformis mosseae, Gigaspora margarita, Paraglomus occultum, and Septoglomus constrictum. No differences were noted between the AMF isolated from Dongdo and Seodo in Dokdo Island, and all of these AMF have been reported previously in Korea. These results could be useful for diversity and functional analyses of AMF in Korea.

• Journal of Ginseng Research
• /
• v.33 no.4
• /
• pp.256-259
• /
• 2009

The effects of benomyl treatment on ginsenoside and arbuscular mycorrhizal (AM) symbiosis in the roots of Panax ginseng that were collected from two sites in Korea were investigated. The ginseng roots that were treated with benomyl showed different species compositions of AM fungi colonizing the ginseng roots, compared to untreated roots. In the analysis of ginsenoside, Rc was significantly higher in benomyl untreated roots than in benomyl treated roots. The results suggest that AM fungal species composition and ginsenosides in ginseng root could be influenced by the benomyl treatment.

• Mycobiology
• /
• v.42 no.4
• /
• pp.416-421
• /
• 2014

In this study, we examined arbuscular mycorrhizal fungal (AMF) community structure colonizing field-cultivated ginseng roots according of different ages, such as 1- to 5-year-old plant, collected from Geumsan-gun, Korea. A total of seven AMF species namely, Funnelliformis caledonium, F. moseae, Gigaspora margarita, Paraglomus laccatum, P. occultum, Rhizophagus irregularis, and Scutellospora heterogama were identified from the roots using cloning, PCR-restriction fragment length polymorphism and sequence analysis of the large subunit region in rDNA. AMF species diversity in the ginseng roots decreased with the increase in root age because of the decreased species evenness. In addition, the community structures of AMF in the roots became more uniform. These results suggest that the age of ginseng affects mycorrhizal colonization and its community structure.