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Diversity of Arbuscular Mycorrhizal Fungi Isolated from Dokdo Island

독도의 식물 근권에 분포하는 수지상균근균의 다양성

  • Eo, Ju-Kyeong (Bureau of Ecological Research, National Institute of Ecology) ;
  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Choi, Seung-Se (Bureau of Ecological Research, National Institute of Ecology) ;
  • Shin, Hyun-Chul (Bureau of Ecological Research, National Institute of Ecology) ;
  • Song, Se-Kyu (Bureau of Ecological Research, National Institute of Ecology) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 어주경 (국립생태원 생태연구본부) ;
  • 박혁 (한국교원대학교 생물교육과) ;
  • 최승세 (국립생태원 생태연구본부) ;
  • 신현철 (국립생태원 생태연구본부) ;
  • 송세규 (국립생태원 생태연구본부) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2017.11.13
  • Accepted : 2017.11.16
  • Published : 2017.12.01

Abstract

In this study, arbuscular mycorrhizal fungi (AMF) were isolated from rhizosphere soils of Dokdo Island. Based on their morphological characteristics and 18S rDNA sequence analysis, eight species belonging to seven genera were identified: Acaulospora longula, A. mellea, Claroideoglomus claroideum, Diversispora aurantia, Funneliformis mosseae, Gigaspora margarita, Paraglomus occultum, and Septoglomus constrictum. No differences were noted between the AMF isolated from Dongdo and Seodo in Dokdo Island, and all of these AMF have been reported previously in Korea. These results could be useful for diversity and functional analyses of AMF in Korea.

본 연구에서는 독도의 근권에 분포하는 수지상균근균을 분리 배양하였다. 형태적 특징과 18s rDNA 염기서열에 의거하여 총 7속 8종의 수지상균근균을 동정하였다; Acaulospora longula, Acaulospora mellea, Claroideoglomus claroideum, Diversispora aurantia, Funneliformis mosseae, Gigaspora margarita, Paraglomus occultum, Septoglomus constrictum. 동도와 서도의 종 다양성에는 차이가 없었으며, 한반도에서 발견한 것과 동일한 분류군들을 확인하였다. 본 연구는 독도의 수지상균근균 다양성 연구와 추후 수지상균근균의 기능규명에 도움을 줄 것으로 판단된다.

Keywords

References

  1. Redecker D, Morton JB, Bruns TD. Ancestral lineages of arbuscular mycorrhizal fungi (Glomales). Mol Phylogenet Evol 2000;14:276-84. https://doi.org/10.1006/mpev.1999.0713
  2. Finlay RD. Ecological aspects of mycorrhizal symbiosis: with special emphasis on the functional diversity of interactions involving the extraradical mycelium. J Exp Bot 2008;59:1115-26. https://doi.org/10.1093/jxb/ern059
  3. Eo JK, Eom AH. The effect of benomyl treatments on ginsenosides and arbuscular mycorrhizal symbiosis in roots of Panax ginseng. J Ginseng Res 2009;33:256-9. https://doi.org/10.5142/JGR.2009.33.4.256
  4. Smith SE, Smith FA. Structure and function of the interfaces in biotrophic symbioses as they relate to nutrient transport. New Phytol 1990;114:1-38. https://doi.org/10.1111/j.1469-8137.1990.tb00370.x
  5. Huh S, Park CH, Yoo HS, Han SJ. Geological structure and depositional environments in the Dokdo Island, East Sea. Geophys Geophys Explor 2005;8:131-5.
  6. Lee DH, Cho SH, Pak JH. The analysis of vascular plant species composition in Dok-do Island. Korean J Plant Taxon 2007;37:545-63. https://doi.org/10.11110/kjpt.2007.37.4.545
  7. Park HS, Park RS, Myoung JG. Vertical distribution of mega-invertebrate and calculation to the stock assessment of commercial species inhibiting shallow hard-bottom in Dokdo, Korea. Ocean Polar Res 2002;24:457-64. https://doi.org/10.4217/OPR.2002.24.4.457
  8. Myoung JG. The fish fauna around Dokdo in the East Sea, Korea. Ocean Polar Res 2002;24:449-55. https://doi.org/10.4217/OPR.2002.24.4.449
  9. Yoon JH, Kang SJ, Lee SY, Lee MH, Oh TK. Virgibacillus dokdonensis sp. nov., isolated from a Korean island, Dokdo, located at the edge of the East Sea in Korea. Int J Syst Evol Microbiol 2005;55:1833-7. https://doi.org/10.1099/ijs.0.63613-0
  10. You YH, Yoon HJ, Lee GS, Woo JR, Rim SO, Shin JH, Lee IJ, Choo YS, Kim JG. Diversity and plant growth-promotion of endophytic fungi isolated from the roots of plants in Dokdo Islands. J Life Sci 2011;21:992-6. https://doi.org/10.5352/JLS.2011.21.7.992
  11. Schenck NC. Methods and principles of mycorrhizal research. St. Paul: APS Press; 1982.
  12. Schussler A, Walker C. The Glomeromycota: a species list with new families and new genera. Richimond: Royal Botanic Gardens, Kew; 2010.
  13. van Tuinen D, Jacquot E, Zhao B, Gollotte A, Gianinazzi-Pearson V. Characterization of root colonization profiles by a microcosm community of arbuscular mycorrhizal fungi using 25S rDNA-targeted nested PCR. Mol Ecol 1998;7:879-87. https://doi.org/10.1046/j.1365-294x.1998.00410.x
  14. Jacquot E, van Tuinen D, Gianinazzi S, Gianinazzi-Pearson V. Monitoring species of arbuscular mycorrhizal fungi in planta and in soil by nested PCR: application to the study of the impact of sewage sludge. Plant Soil 2000;226:179-88. https://doi.org/10.1023/A:1026475925703
  15. Lee J, Lee S, Young JP. Improved PCR primers for the detection and identification of arbuscular mycorrhizal fungi. FEMS Microbiol Ecol 2008;65:339-49. https://doi.org/10.1111/j.1574-6941.2008.00531.x
  16. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for Bigger Datasets. Mol Biol Evol 2016;33:1870-4. https://doi.org/10.1093/molbev/msw054
  17. Park H, Lee EH, Ka KH, Eom AH. Community structures of arbuscular mycorrhizal fungi in soils and plant roots inhabiting abandoned mines of Korea. Mycobiology 2016;44:277-82. https://doi.org/10.5941/MYCO.2016.44.4.277
  18. Park SH, Eo JK, Ka KH, Eom AH. Diversity of arbuscular mycorrhizal fungi of woody plants in Mt. Munan. Kor J Mycol 2011;39:1-6. https://doi.org/10.4489/KJM.2011.39.1.001
  19. Maia LC, Trufem SF. Vesicular-arbuscular mycorrhizal fungi in cultivated soils in Pernambuco State, Brazil. Rev Bras Bot 1990;13:89-96.
  20. Lee EH, Lee JY, Eo JK, Ka KH, Eom AH. Notes on some unrecorded species of arbuscular mycorrhizal fungi collected from rhizospheres of plants in Korea. Kor J Mycol 2014;42:306-11. https://doi.org/10.4489/KJM.2014.42.4.306
  21. Eom AH, Lee SS. Endomycorrhizal fungi identified on the soils in forest and coast areas. Kor J Mycol 1989;17:14-20.
  22. Bentivenga SP, Morton JB. A monograph of the genus Gigaspora, incorporating developmental patterns of morphological characters. Mycologia 1995;87:719-31. https://doi.org/10.2307/3760818
  23. Eom AH, Lee SS. Endomycorrhizal fungi found from the soils of the communities of Persicaria thunbergii H. Gross. Kor J Mycol 1990;18:26-41.
  24. Wu QS, Zou YN, He XH. Contributions of arbuscular mycorrhizal fungi to growth, photosynthesis, root morphology and ionic balance of citrus seedlings under salt stress. Acta Physiol Plant 2010;32:297-304. https://doi.org/10.1007/s11738-009-0407-z
  25. Koh SD, Lee HH. Studies of species and distribution of vesicular-arbuscular mycorrhizal fungi in relation to salt-marsh plants. Kor J Mycol 1984;12:175-82.
  26. Lee SS, Eom AH, Lee OH, Kim MK, Kim SI. Descriptions of some arbuscular mycorrhizal fungi produced under artificial conditions and collected in Korea. Kor J Mycol 1993;21:85-93.
  27. Lee EH, Ka KH, Eom AH. Diversity of arbuscular mycorrhizal fungi in rhizospheres of Camellia japonica and neighboring plants inhabiting Wando of Korea. Kor J Mycol 2014;42:34-9. https://doi.org/10.4489/KJM.2014.42.1.34
  28. Kim MH, Oh YJ, Kim CS, Han MS, Lee JT, Na YE. The flora and vegetation distribution in Dokdo. Korean J Environ Agric 2007;26:85-93. https://doi.org/10.5338/KJEA.2007.26.1.085
  29. Jeong SJ, Eo JK, Eom AH. Identification of spores of arbuscular mycorrhizal fungi isolated from soils of rhizosphere of Ulleung-do. J Nat Sci 2016;6:11-6.