• Journal of Veterinary Clinics
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• v.36 no.3
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• pp.133-138
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• 2019

The present study was performed to identify the nematodes recovered from the Eurasian jay, Garrulus glandarius brandtii, from Daejeon Metropolitan City, the Republic of Korea. Total five nematode worms were detected in the body cavities of two out of the twenty birds necropsied, and they were identified using morphological features, light and scanning electron microscope (SEM), and molecular (18S rRNA analysis) methods. The nematodes were all female Diplotriaena manipoli and had numerous eggs at different developmental stages in the uterus. The nematodes were long and slender measuring about 123-145 mm. The eight submedian cephalic papillae were arranged into four large, outer papillae and four small, inner-circle papillae. A pair of amphidal pores were located at the lateral portion of the mouth. The manubrium apex of trident was rounded and three branches of trident were bluntly rounded at the posterior ends. Using 18S rRNA partial sequence arrangements, DNA analysis of nematode worms was also carried out, and they were identified to be close to the Serratospiculum tendo based on a phylogenic tree analysis. To our knowledge, this is the first report on the molecular characterization and SEM study of D. manipoli.

• Journal of Animal Science and Technology
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• v.54 no.5
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• pp.355-361
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• 2012

The present study was conducted to investigate the effects of concentrate feeding on rumen papillae development in suckling Hanwoo calves before weaning (60 days of age). Twenty-four Hanwoo calves (12 heifers and 12 bulls) at six days of age were randomly assigned to one of three dietary treatments [hay and two levels (low and high) of concentrate feeding] and given each diet with free access to their dams for suckling until 60 days of age. At 60 days of age, two calves from each treatment were sacrificed and used for post-mortem examination of rumen papillae development. Feed intake between 31 and 60 days of age was significantly higher (P<0.05) for the high concentrate treatment (380.2 g/d) than for other treatments (58.3 and 76.9 g/d for hay and low concentrate treatments, respectively). Although feed intake showed a large difference between the low and high concentrate treatments, body weights at birth and 60 days of age were similar between the two treatments, suggesting that calves in the low concentrate feeding suckled more milk from their dams than those in the high concentrate feeding. The ratio of reticulo-rumen weight to body weight at 60 days of age was significantly increased (P<0.05) in the high concentrate (1.39%), compared with those in the hay (0.85%) and low concentrate (1.06%) treatments. Furthermore, the high concentrate feeding significantly increased both rumen papillae length and width, compared with the hay and low concentrate feeding group. This was also detected clearly by visual observation. The blood concentration of ${\beta}$-hydroxybutyrate was significantly higher for the high concentrate (176.4 ${\mu}mol/L$) than for other treatments (58.9 and 59.2 ${\mu}mol/L$ for the hay and the low concentrate group, respectively). In conclusion, the results of the present study showed that, to achieve a large development of rumen papillae before weaning (60 days of age) in suckling Hanwoo calves, the amount of concentrate intake should be important. And also the results implied that hay might not be included in the diet for suckling Hanwoo calves before weaning (60 days of age).

• Korean Journal of Plant Taxonomy
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• v.42 no.4
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• pp.247-252
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• 2012

Seed morphology in the subtribe Agrimoniinae (Rosaceae) was examined using scanning electron microscopy to identify distinct characters and evaluate their evolution in a phylogenetic framework for five genera in the subtribe: Agrimonia L., Aremonia Neck. ex Nestl., Hagenia J.F. Gmel., Leucosidea Eckl. & Zeyh., and Spenceria Trimen. All genera have one or two mature achenes in a fruiting hypanthium. In the seed coats, the cell shape, size, wall features, and sculpturing vary across genera. Of most significance is the presence of papillae structures in both Agrimonia and Aremonia. Through the mapping of papillae features onto phylogenetic trees, either one or two changes in seed coats are hypothesized. The phylogenetic tree inferred from four nuclear and six chloroplast regions of sequence data suggests that at least two steps of papillae sculpturing on seed coats are required. On the other hand, in the phylogenetic tree of a low-copy nuclear gene, one independent evolutionary step is postulated to explain the current character states. In the latter hypothesis, the seed coat sculpturing also supports a monophyletic relationship for cosmopolitan Agrimonia and European endemic Aremonia. The seed coat sculpturing provides valuable information for inferring phylogenetic relationships at the generic level in the subtribe Agrimoniinae.

• Journal of Periodontal and Implant Science
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• v.40 no.3
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• pp.139-143
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• 2010

Purpose: Pink gingival esthetic especially on the anterior teeth has been an important success criterion in implant-supported restoration. Inter-implant papillae are a critical factor for implant esthetics, and various techniques for inter-implant papilla reconstruction have been introduced. The aim of this study is to suggest and evaluate a surgical technique for reconstructing inter-implant papillae. Methods: A 28-year-old man had an implant placed on the #13 and #14 area. Four months after implant placement, a second stage surgery was planned for inter-implant papilla reconstruction. At the time of the abutment connection, I-type incisions were performed on the #13i & #14i area followed by full-thickness flap elevation and connection of a healing abutment on underlying fixtures without suture. Results: Two weeks after the second stage implant surgery, soft tissue augmentation between the two implants was achieved. Conclusions: I-shaped incisions for papilla reconstruction performed during the second stage implant surgery were useful for inter-implant papilla reconstruction and showed a good esthetic result.

• Parasites, Hosts and Diseases
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• v.26 no.2
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• pp.113-116
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• 1988

The pattern of sensory papillae, chaetotaxy, of the cercaria of Clenorchis sinensis was observed, The chaetotaxy was as follows; 5~6 Ci 1, 4~5 Ci 2, 5, ~6 Ci3 at 1st row, 4 Cii 1, 2 Cii2, 4 Cii3, 5~6 Cii4 at 2nd row, 3~4 Ciii 1, 2~3 Ciii 2 at 3rd row, and 2 Civl, 2~3civ2, 2~3 Civ 3, at 4th row, in cephalic region; 2 AiV, 1 AiD, 2 AiiV, 1 AiiD, 2 Aiiiv, 2 AiiiD, 1 AivV, 1 AivD, 1 PiiD, 1 PiiiD, in ventral(V) and dorsal(D) portions of body. Caudal region revealed 2-2-2-2 formula.

• Korean Journal of Veterinary Research
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• v.36 no.2
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• pp.265-276
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• 1996

This study was performed to identify the localization of several neuropeptides; calcitonin gene-related peptide(CGRP), substance P(SP), vasoactive intestinal polypeptide(VIP), neuropeptide Y(NPY), serotonin(5-HT) and neurotensin in the tongue of Korean native goat(Capra hircus) by immunohistochemical method. The results were summarized as follows: CGRP- and SP-immunoreactive fibers were observed as moderate immunoreactivity at the subepithelial plexus and subgemmal fibers in lamina propria of lingual papillae, but not seen in intragemmal, intergemmal, perigemmal fibers as well as in the supporting, basal and taste cells. Fibers around the acinus of the von Ebner's gland and blood vessels showed weak immunoreactivities against CGRP and SP. In the intrinsic ganglion cells, CGRP- and SP-immunoreactivities were not observed. The distribution patterns of VIP- and NPY-immunoreactive fibers were similar to CGRP-or SP-immunoreactive fibers, but their immunoreactivities were stronger than those of CGRP- or SP immunoreactive fibers. The immunoreactivities to VIP or NPY were seen in the intrinsic ganglion. Only a few serotonin immunoreactive fibers were seen in some filiform or fungiform papillae. Neurotensin immunoreactivity was not observed in the tongue of Korean native goat.

• Parasites, Hosts and Diseases
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• v.50 no.2
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• pp.177-180
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• 2012

Cercaria caribbea LVIII Cable, 1963 (Digenea: Cyathocotylidae) was detected from a brackish water gastropod species (Cerithideopsilla cingulata) in a coatal area of Shinan-gun, Jeollanam-do (Province), the Republic of Korea, and its surface ultrastructure was studied using a scanning electron microscope. The cercariae were found freely swimming or enveloped within daughter sporocysts when the snail host was mechanically broken. They were morphologically characterized by a linguiform and ventrally concave body, a long and bifurcated tail, and the presence of a holdfast (=tribocytic) organ posterior to the ventral sucker. On the whole ventral and dorsal surfaces, peg-like tegumental spines were densely distributed. Around the oral sucker, several sensory papillae, each with a short cilium, were distributed, and on the tail, sensory papillae, each with an extensively long cilium, were observed. This is the first record describing a cyathocotylid cercaria from a brackish water gastropod in the Republic of Korea.

• Korean Journal of Veterinary Research
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• v.45 no.1
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• pp.99-103
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• 2005

We report septicemic colibacillosis accompanied by white-spotted kidney in a 30-day old female Holstein-Friesian dairy calf. Grossly, there were numerous white spots sized average 0.5cm in diameter in both kidneys. When sectioned sagittally, there were radially oriented gray streaks extending outward and reaching the renal cortex. The renal papillae were ulcerated, white to gray in color and very friable. Histologically, there was extensive purulent inflammation characterized by severe neutrophilic cellular infiltrations in the tubular lumens and interstitia. In addition, massive coagulative necrosis were found in the apices of papillae. Numerous Gram-negative bacterial colonies were detected in both of the renal and lung tissues. Beta-hemolytic Escherichia coli (E. coli) was purely isolated from the renal parenchyma, peritoneal surface and pleural surface. Based on the above results, we suggest that the generalized septicemia with pyelonephritis may be oriented from the lower urinary tract infection with E. coli in this case.

• Journal of Species Research
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• v.8 no.3
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• pp.259-267
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• 2019

Five free-living nematode species belonging to the order of Rhabditida are described for the first time in Korea. Two unrecorded species of Rhabditidae, Cruznema tripartitum (Linston, 1906) Zullini, 1982, and Poikilolaimus oxycerca de Man, 1895, and one species of Peloderidae, Pelodera strongyloides Schneider, 1860, were collected in Korea. C. tripartitum has nine pairs of genital papillae arranged in 2+2+1+4 differently from similar species of C. scarabaeum which has ten genital papillae arranged in 2+1+4+3. Poikilolaimus oxycerca has shorter tail (shorter than anal body diameter) compared to similar species of P. regenfussi which has longer tail(longer than anal body diameter). Pelodera strongyloides has shorter tail(1-1.5 times anal body diameters long) than P. punctata (4 anal body diameters long). Two unrecorded species of Panagrolaimidae, Panagrolaimus apicatus Schuurmans Stekhoven & Teunissen, 1938 and Panagrolaimus rigidus (Schneider, 1866) Thorne, 1937 were collected in Korea. P. apicatus has rounded and lower lip compared to similar species, P. margaretae Massey, 1964 which has finely pointed lip. P. rigidus has slender tail and lips rounded and flat barely separated when compared to similar species, P. subelongatus(Cobb, 1914) Thorne, 1937 which has plumper tail and lips are well separated.

• Parasites, Hosts and Diseases
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• v.60 no.1
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• pp.45-49
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• 2022

The Japanese amberjack Seriolae quinqueradiata is one of the most consumed fish species among the Koreans. However, information regarding parasitic infection in Japanese amberjack is scarce. This study described the morphological and molecular characteristics of a species of philometrid nematode, Philometroides seriolae, which was recovered from Japanese amberjack. This fish was caught in the sea of Goseong-gun, Gangwon-do, Republic of Korea (Korea). Six P. seriolae (Nematoda: Philometridae) were recovered from 2 Japanese amberjacks. These parasites were subgravid female which were 325-420 mm long and 2.95-3.27 mm wide. Furthermore, they had typical papillae distributed on their body surface with 14 papillae at the apical view. Sequence analysis of the small subunits of ribosomal RNA (SSU rRNA) showed high sequence identity (99.8%, 1,607/1,611-bp) with that of P. seriolae (GenBank accession no. FJ155811). This nematode species has been newly added to the Korean nematode fauna.