• Title/Summary/Keyword: Paper-based ELISA

Search Result 13, Processing Time 0.018 seconds

Simplified elastic design checks for torsionally balanced and unbalanced low-medium rise buildings in lower seismicity regions

  • Lam, Nelson T.K.;Wilson, John L.;Lumantarna, Elisa
    • Earthquakes and Structures
    • /
    • v.11 no.5
    • /
    • pp.741-777
    • /
    • 2016
  • A simplified approach of assessing torsionally balanced (TB) and torsionally unbalanced (TU) low-medium rise buildings of up to 30 m in height is presented in this paper for regions of low-to-moderate seismicity. The Generalised Force Method of Analysis for TB buildings which is illustrated in the early part of the paper involves calculation of the deflection profile of the building in a 2D analysis in order that a capacity diagram can be constructed to intercept with the acceleration-displacement response spectrum diagram representing seismic actions. This approach of calculation on the planar model of a building which involves applying lateral forces to the building (waiving away the need of a dynamic analysis and yet obtaining similar results) has been adapted for determining the deflection behaviour of a TU building in the later part of the paper. Another key original contribution to knowledge is taking into account the strong dependence of the torsional response behaviour of the building on the periodic properties of the applied excitations in relation to the natural periods of vibration of the building. Many of the trends presented are not reflected in provisions of major codes of practices for the seismic design of buildings. The deflection behaviour of the building in response to displacement controlled (DC) excitations is in stark contrast to behaviour in acceleration controlled (AC), or velocity controlled (VC), conditions, and is much easier to generalise. Although DC conditions are rare with buildings not exceeding 30 m in height displacement estimates based on such conditions can be taken as upper bound estimates in order that a conservative prediction of the displacement profile at the edge of a TU building can be obtained conveniently by the use of a constant amplification factor to scale results from planar analysis.

Current Status and Developments of Comparative Education at Universities in Korea and World Wide (세계적 동향에 비추어 본 우리나라 대학의 비교교육학 교육현황과 과제)

  • Hyun, Kyung S.;Park, Elisa L.
    • Korean Journal of Comparative Education
    • /
    • v.18 no.4
    • /
    • pp.1-26
    • /
    • 2008
  • The purpose of this study is to present the overall landscape of Comparative Education study in teacher training program at universities in Korea. The Comparative Education in Korea is relatively young without a lengthy tail on its name and still on the way of upgrading itself, however various efforts are gathering for the development of Comparative Education. With this understanding, this paper diagnoses the current status of Comparative Education in Korea by analyzing 1) the number of institutions which provide Comparative Education lectures for their teacher training program, 2) aims of Comparative Education teaching, 3) curricular and course titles, 4) teaching methods and textbooks, and 5) current issues and problems currently laid on Comparative Education in Korea designated by actual lecturers and professors who are in charge of Comparative Education lectures. The historical development of Comparative Education in Korea will be briefly presented at the beginning part of the chapter. To gauge the comparative position of Korean Comparative Education, the second half of this paper introduces the cases of Comparative Education at universities worldwide, presenting various countries' status of Comparative Education along with their developmental status. As the first research which pays an attention on the current figure of Comparative Education in Korea, it will form an impetus on why we should teach college students about comparative education and on what next step should be carried for further development of Comparative Education at universities based on the comparative understanding of other countries. Since it is in the transitional stage in terms of its development, it would be critical to have a self-analysis of the current status, which will help to lay the comprehensive understanding background for designating the future direction of its development.

Isolation and Characterization of Pepper mottle virus Infecting Tomato in Korea

  • Kim, Mi-Kyeong;Kwak, Hae-Ryun;Han, Jung-Heon;Ko, Sug-Ju;Lee, Su-Heon;Park, Jin-Woo;Jonson, Miranda Gilda;Kim, Kook-Hyung;Kim, Jeong-Soo;Choi, Hong-Soo;Cha, Byeong-Jin
    • The Plant Pathology Journal
    • /
    • v.24 no.2
    • /
    • pp.152-158
    • /
    • 2008
  • A peculiar virus-like disease of tomato showing yellow mosaic and necrotic spots on leaves and necrosis on veins, petioles and stems was observed at the Tomato Experimental Station (TES), Buyeo, Chungcheongnamdo, Korea. The disease incidence at TES fields ranged from 21 to 35% infecting different tomato cultivars. For this reason, to identify the virus infecting tomato and to characterize the virus based on biology, serology, cytology and at molecular level. Here, leaf samples were randomly collected from different infected tomato cultivars at TES fields and greenhouses and tested by ELISA using Pepper mottle virus (PePMoV) and Tomato mosaic virus (ToMV) antisera. Infected saps were mechanically inoculated in different host plants to test for pathogenicity, symptomatology and host ranges. Infected tissues and ultrathin sections were examined by electron microscopy. Finally, putative coat protein and 3'-untranslated region (CP/3'-UTR) fragment was amplified and cloned for sequence determination and analyzed its genetic relationship to existing PepMoV and PVY sequences at the Genbank. Results showed 69% of the samples were positive with PepMoV, 13% with ToMV and 19 % were doubly infected with PepMoV and ToMV. Symptoms greatly varied from different host plants inoculated with tomato leaf sap infected with PepMoV alone and discussed in detailed in this paper. Electron microscopy from infected tissues showed filamentous particles of 720-750nm in length, a typical morphology and size of PepMoV. In addition, cylindrical inclusion bodies, pinwheels, scrolls and laminates with masses of fibrillar inclusions were also found in ultrathin sections. Alignment of the sequences of the CP/3'-UTR revealed >96% sequence identity with PepMoV and only <61% with PVY. Taken together, all these evidences presented clearly indicated that the causal agent infecting tomato at TES was PepMoV and we designated this PepMoV infecting tomato as Tom-sd2 strain in this study.